lonza,CC-4461,R-CB-503,CC-3256,PNGM™ 原代神经元生长培养基 BulletKit™

Catalog #: CC-4461

含有 PNBMTM 基础培养基 (CC-3256) 和 PNGMTM SinglequotsTM 生长补充剂 (CC-4462) 的培养系统,是啮齿动物原代神经元生长所需的

Product Overview

CloneticsTM PNGMTM Primary Neuron Growth Medium Kit (PNGMTM) was specifically developed for culturing embryonic primary rat and mouse neurons. The media kit is recommended for use with all of our primary rat and mouse neurons.

CloneticsTM PNGMTM 原代神经元生长培养基试剂盒 (PNGMTM) 专为培养胚胎原代大鼠和小鼠神经元而开发。 建议将媒体套件与我们所有的主要大鼠和小鼠神经元一起使用。

Benefits

  • Serum-free medium
  • Allows for long-term maintenance and growth of neuronal cells
  • Promotes development of high quality neuronal networks without the need of an astrocyte feeder layer

Content & Storage

Content

1 x PNBMTM Basal Medium (CC-3256), 200 mL

1 x PNGMTM SinglequotsTM Growth Supplement Pack (CC-4462)

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ViaLightPlus Assay in PNGM™ Media of Rat Cortical Neurons

Comparison of survival of Clonetics™ Primary Rat Cortical Neurons cultured in PNGM™ Medium vs. Company A’s medium. Cryopreserved cells were thawed and plated in laminin-coated, 96 well plates at ~1000 cells per square mm (40% of the recommended seeding density) using either PNGM™ Medium or Company A’s medium. Cells were cultured without feeding for six days, at which time cell viability was assessed using the ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay Kit. Results represent the average of 8 experiments.

PNGM™ Primary Neuron Growth Medium BulletKit™
在 PNGM™ 培养基与 A 公司培养基中培养的 Clonetics™ 原代大鼠皮质神经元的存活率比较。 使用 PNGM™ 培养基或 A 公司的培养基,将冷冻保存的细胞解冻并接种在层粘连蛋白涂层的 96 孔板中,每平方毫米约 1000 个细胞(推荐接种密度的 40%)。 细胞在不喂食的情况下培养六天,此时使用 ViaLight™ Plus 细胞增殖和细胞毒性生物测定试剂盒评估细胞活力。 结果代表 8 次实验的平均值。

lonza,R-DRG-505,CC-4461,R-EDRG-515,大鼠新生儿背根神经节 (DRG) 神经元

Catalog #: R-DRG-505

Cryopreserved ampule of Rat Neonatal DRG Neurons containing ≥ 200,000 cells

Product Overview

即用型 CloneticsTM 大鼠背根神经节神经元是通过标准化方法制备的高质量感觉神经元的悬浮液,可立即进行培养。

每瓶背根神经节细胞在 0.25 ml 悬浮液中含有大约 200,000 个细胞。电镀后最初几天会发生细胞死亡,并观察到碎片。这很正常。培养约 4 天后,细胞将形成神经突网络,到第 7 天,碎片将最少。

在没有有丝分裂抑制剂的情况下,神经元倾向于聚集(神经节化)并从底物上脱离。神经节是形成神经中枢的一组神经细胞,尤其是位于大脑或脊髓外的神经细胞。

背根神经节,也称为脊神经节,是每个脊神经节段神经后根的神经节,包含单极初级感觉神经元的细胞体。背根神经节细胞是假单极细胞。假单极细胞有 2 个轴突,而不是一个轴突和树突。一个轴突向脊髓中央延伸;另一个轴突向皮肤或肌肉延伸。

推荐介质:PNGMTM BulletKitTM(CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥200,000 cryopreserved cells per ampule

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