Culture system containing BEBMTM Bronchial Epithelial Cell Growth Basal Medium (CC-3171) and BEGMTM Bronchial Epithelial Cell Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4175).
Request Information or Quote or do you need a different format or volume for this media product? Contact Lonza CellBio Services to discuss custom format and formulation options.
包含 BEBMTM 支气管上皮细胞生长基础培养基 (CC-3171) 和 BEGMTM 支气管上皮细胞生长培养基 SingleQuotsTM 添加剂和生长因子 (CC-4175) 的培养系统。
请求信息或报价，或者您是否需要此媒体产品的不同格式或音量？ 联系 Lonza CellBio Services 讨论定制形式和配方选项。
Product Overview
BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.
Published research shows BEGMTM BulletKitTM has expanded applications. Our customers have used BEGMTM BulletKitTM to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the ‘Data’ tab.
BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGMTMBulletKitTM Media may also offer cost-benefit since customers can use the same media to support multiple cell types.
BEGMTM 支气管上皮细胞生长培养基专为支持人原代支气管上皮细胞的生长而设计。 BEGMTM BulletKitTM 不含血清，经过优化，可从正常和患病组织中培养细胞。
已发表的研究表明 BEGMTM BulletKitTM 已经扩展了应用程序。我们的客户已使用 BEGMTM BulletKitTM 培养成熟的细胞系，如 BEAS-2B、本土支气管上皮细胞，并支持与乳腺癌细胞系如 MCF-7 和 MDA-MB-231 的共培养模型。请参阅“数据”选项卡中已发表的文献。
BEGMTM 是一种经过优化且随时可用的培养基套件，可为研究人员提供支持其细胞的系统的信心和便利性。这对于利用细胞系作为原代细胞培养垫脚石的研究人员来说尤其重要。在细胞系和原代 NHBE 之间使用相同的 BEGMTM 培养基可以减少实验中的变异性并简化研究人员的转变。此外，如果将原代细胞用作与正常细胞系或本土支气管上皮细胞并排的对照，BEGMTMBulletKitTM 培养基还可以提供成本效益，因为客户可以使用相同的培养基来支持多种细胞类型。
Here are publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells:
- El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells
- Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza’s HUVEC and NHBE in a 3D environment.
- Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs
- El Najjar 等人。 （2015 年）。 PLoSONE 10(2) 展示了在 BEGMTM 培养基中培养 BEAS-2B 支气管上皮细胞系以检查组织蛋白酶和弗林蛋白酶对细胞中病毒融合蛋白活化的影响
萩原等人。 （2015 年）。 Nature 证明 BEGMTM Media 可以理想地支持 Lonza 的 HUVEC 和 NHBE 在 3D 环境中的共培养。
骑士，等人。 （2015 年）。 PLoS ONE 10(1) 使用 BEGMTM 培养基培养从非移植的正常肺获得的原代人支气管上皮细胞
Purchase our Bronchial Epithelial Cell Culture Kit (catalog#. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog #CC-2540) and BEGMTM BulletKitTM Growth Media (catalog#. CC-3170).
购买我们的支气管上皮细胞培养试剂盒（catalog#. CC-2540B）并获得细胞和培养基的折扣价。 该试剂盒由冷冻保存的支气管上皮细胞安瓿（产品目录号#CC-2540）和 BEGMTM BulletKitTM 生长培养基（目录号#CC-3170）组成。
Content & Storage
Content
1 x BEBMTM Basal Medium (CC-3171), 500 mL
1 x BEGMTM SingleQuotsTM Supplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
Related Products
Bronchial Epithelial Cells in RAFT™ 3D Cell Culture System
Bronchial Epithelial Cells Grown in BEGM™ BulletKit™ and Cultured in RAFT™ 3D Cell Culture System
Co-culture Experiments with Lonza’s Bronchial Epithelial Cells and BEGM™ BulletKit™ Media
Lonza支气管上皮细胞和 BEGM™ BulletKit™ 培养基的共培养实验
Furukawa, et al. demonstrates BEGM™ BulletKit™ is suitable for culturing Lonza’s NHBEs as well as to support co-cultures with lung epithelial cell line, BEAS-2B, and breast cancer cell lines, MCF-7 and MDA-MB-231. The breast cancer cell line showed differences in morphology and proliferation rates when cultured with primary NHBE cells vs. BEAS-2B cell line. The paper highlights the importance of using primary NHBE cells as a side-by-side control when conducting experiments with normal lung epithelial cell line such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines.
古川等人。 演示 BEGM™ BulletKit™ 适用于培养 Lonza 的 NHBE，并支持与肺上皮细胞系 BEAS-2B 和乳腺癌细胞系 MCF-7 和 MDA-MB-231 的共培养。 当与原代 NHBE 细胞与 BEAS-2B 细胞系一起培养时，乳腺癌细胞系在形态和增殖率方面表现出差异。 本文强调了在对正常肺上皮细胞系（如 BEAS-2B）进行实验时使用原代 NHBE 细胞作为并排对照的重要性，因为未转化的原代细胞可以验证或抵消使用细胞系生成的数据。
