Culture system containing MEBMTM Basal Medium (CC-3151) and MEGMTM SingleQuotsTM Supplements (CC-4136) required for growth of Mammary Epithelial Cells.
包含乳腺上皮细胞生长所需的 MEBMTM 基础培养基 (CC-3151) 和 MEGMTM SingleQuotsTM 补充剂 (CC-4136) 的培养系统。
Product Overview
MEGMTMBulletkitTM, is also sold as separate basal medium and supplements.
MEGMTMTM Mammary Epithelial Cell Growth Medium has been optimized for the growth of Mammary Epithelial cells in a serum-free environment. This media system has been referenced in over 130 unique publications primarily focusing on breast cancer research.
MEGMTMBulletkitTM 也作为单独的基础培养基和补充剂出售。
MEGMTMTM 乳腺上皮细胞生长培养基针对乳腺上皮细胞在无血清环境中的生长进行了优化。 该媒体系统已被 130 多种主要关注乳腺癌研究的独特出版物引用。
Did you know our customers have used MEGMTMTM BulletKitTM not only for Lonza’s primary HMECs but also to culture well-established normal breast cell lines such as MCF-10A and MCF-12A?
MEGMTMTM BulletKitTM is optimized and readily available media kit giving researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing normal breast cell lines as a stepping stone to primary HMEC cell culture. Working with the same media between cell lines and primary cells can reduce variability within the experiments and simplifies the shift for researchers. In addition, if primary HMECs are utilized as a side-by-side control with normal breast cell lines, the MEGMTMTM BulletKitTM media may also offer cost-benefits since customers can use the same media to support both cell types.
Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGMTMTM BulletKitTM Growth Media (catalog no. CC-3150)
MEGMTMTM BulletKitTM 是经过优化且随时可用的培养基套件，为研究人员提供了支持其细胞的系统的信心和便利性。 这对于使用正常乳腺细胞系作为初级 HMEC 细胞培养的垫脚石的研究人员来说尤其重要。 在细胞系和原代细胞之间使用相同的培养基可以减少实验中的变异性并简化研究人员的转变。 此外，如果将原代 HMEC 用作与正常乳腺细胞系的并排对照，MEGMTMTM BulletKitTM 培养基还可以提供成本效益，因为客户可以使用相同的培养基来支持两种细胞类型。
购买我们的乳腺上皮细胞培养试剂盒（目录号 CC-2551B）并获得细胞和培养基的折扣价。 该试剂盒包括一个冷冻保存的乳腺上皮细胞安瓿（货号 CC-2551）和 MEGMTMTM BulletKitTM 生长培养基（货号 CC-3150）
Content & Storage
Content
1 x MEBMTM Basal Medium (CC-3151), 500 mL
1 x MEGMTM SingleQuotsTM Supplement Pack (CC-4136) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
Safety Data Sheets (SDS)
View SDS
Related Products
MEBM™ 乳腺上皮细胞生长基础培养基 CC-3151
MEGMTM 乳腺上皮细胞生长培养基 SingleQuotsTM 试剂盒 CC-4136
Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems
A Three-Dimensional RAFT™ Co-Culture as Advanced Model for Breast Cancer Drug Discovery
Presented at AACR 2017, we demonstrate here the feasibility of co-culturing human mammary fibroblasts and human mammary epithelial cells in RAFT™ 3D Culture System as a 3D breast cancer model.
Selected publications out of many where our customers have used MEGM™ Growth Media with various normal breast cells.
- Savanur, et al. shows Lonza’s HMECs were cultured in Lonza’s MEGM™ Media alongside breast cancer cell lines in the respective media to understand the inhibitory effects of Lectin on proliferation of breast cancer cells
- Park, et. al. PLoS ONE 10(6) demonstrates how MCF-10A and MCF-12A were cultured in MEGM™™ alongside breast cancer cell lines in their respective media to examine the effects of cold atmospheric plasma on epigenetic alterations
- Kmiecik, et al. 2015. PLoS ONE 10(5) shows MEGM™ Medium’s compatibility with immortalized hTERT-HME1 cells in a study of MT3-overexpression on proliferation, invasiveness, migration,and tumorigenesis of breast cancer cells
- 萨瓦努尔等人。 显示 Lonza 的 HMEC 在 Lonza 的 MEGM™ 培养基中与相应培养基中的乳腺癌细胞系一起培养，以了解凝集素对乳腺癌细胞增殖的抑制作用
公园等 人。 PLoS ONE 10(6) 展示了 MCF-10A 和 MCF-12A 如何在 MEGM™ 中与乳腺癌细胞系一起在各自的培养基中培养，以检查冷大气血浆对表观遗传改变的影响
Kmiecik 等人。 2015. PLoS ONE 10(5) 在 MT3 过表达对乳腺癌细胞增殖、侵袭性、迁移和肿瘤发生的研究中显示了 MEGM™ 培养基与永生化 hTERT-HME1 细胞的相容性
