Cryopreserved ampule of Human Aortic Endothelial Cells (HAEC) containing ≥ 500,000 cells
Product Overview
Cardiac Endothelial Cells include cells isolated from the aorta (HAEC), coronary artery (HCAEC), pulmonary artery (HPAEC), iliac artery (HIAEC), and microvascular cardiac cells (HMVEC-C). Additional donor characteristics including hypertension, or smoking history may be available by contacting Scientific Support. Cardiac fibroblasts from matched donors are available for certain lots.
CloneticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system. Cryopreserved HAECs are shipped in third passage.
Cryopreserved CloneticsTM Human Aortic Endothelial Cells (HAEC) are guaranteed through 15 population doublings, express CD31/105, von Williebrand Factor VIII, and are positive for acetyated low density lipoprotein uptake. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
心脏内皮细胞包括从主动脉 (HAEC)、冠状动脉 (HCAEC)、肺动脉 (HPAEC)、髂动脉 (HIAEC) 和微血管心脏细胞 (HMVEC-C) 分离的细胞。联系科学支持可以获取其他供体特征，包括高血压或吸烟史。来自匹配供体的心脏成纤维细胞可用于某些批次。
CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。冷冻保存的 HAEC 在第三次运输中运输。
冷冻保存的 CloneticsTM 人主动脉内皮细胞 (HAEC) 通过 15 次群体倍增得到保证，表达 CD31/105、von Williebrand 因子 VIII，并且对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
Benefits
- Guaranteed through 15 population doublings
- Test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- A Certificate of Analysis is provided for each cell lot purchased
Content & Storage
Content
1 x Cryopreserved ampule HAEC containing ≥ 500,000 cells
NHCF-A – 人心房心脏成纤维细胞 CC-2903
NHCF-V – 人心室心脏成纤维细胞 CC-2904
AoAF – 人主动脉外膜成纤维细胞 CC-7014
HCAEC – 人冠状动脉内皮细胞 CC-2585
EGM™-2 内皮细胞生长培养基-2 BulletKit™ CC-3162
P5 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-5012
P5 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-5024
P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032
P5 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-5096
P5 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-5960
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5002
HAEC – Human Aortic Endothelial Cells
Human aortic endothelial cells (CC-2535) were immuno histochemically stained with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1094 DyLight 594 (Vector Laboratories). Cells were also satined with CD31/PECAM-1 antibody (R&D Systems, BBA7) used at 1:100 (green). Secondary antibody used to detect was VECTDI2488 DyLight 488 (Vector Laboratories) Hoechst nuclear stain (blue).
用 1:200 使用的血管性血友病因子抗体 (Sigma, F3520) 对人主动脉内皮细胞 (CC-2535) 进行免疫组织化学染色。 使用的二抗是 VECTDI1094 DyLight 594 (Vector Laboratories)。 细胞也用 1:100（绿色）使用的 CD31/PECAM-1 抗体（R&D Systems，BBA7）饱和。 用于检测的二抗是 VECTDI2488 DyLight 488（Vector Laboratories）Hoechst 核染色剂（蓝色）。
HAEC – Human Aortic Endothelial Cells
Human aortic endothelial cells were immunohistochemically stained with VE Cadherin antibody (Santa Cruz,sc-28644) used at 1:200 dilution (red). Secondary antibody used was VECTDI1094 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst stain at 1:10,000 dilution.
