标签：CC-2571

lonza,CC-2571,V5SP-1002,CC-2914,AoSMC-人主动脉平滑肌细胞

Catalog #: CC-2571

Cryopreserved ampule of Human Aortic Smooth Muscle Cells (AoSMC) containing ≥ 500,000 cells

含有 ≥ 500,000 个细胞的人主动脉平滑肌细胞 (AoSMC) 冻存安瓿

Product Overview

Human Vascular Smooth Muscle Cells come from multiple tissue types including Aorta (AoSMC), Coronary Artery (CASMC), Pulmonary Artery (PASMC), and Umbilical Artery (UASMC). Cryopreserved cells are shipped in 3rd passage.

CloneticsTM Human Aortic Smooth Muscle Cells are guaranteed for 15 population doublings and to stain positive for alpha smooth muscle actin and negative for von Willebrand Factor VIII after differentiation. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.

人血管平滑肌细胞来自多种组织类型，包括主动脉 (AoSMC)、冠状动脉 (CASMC)、肺动脉 (PASMC) 和脐动脉 (UASMC)。 冷冻保存的细胞在第 3 代运输。

CloneticsTM 人主动脉平滑肌细胞保证 15 次群体倍增，分化后 α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 染色呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Applications

  • Stroke
  • Diabetes
  • Vascular research
  • Artherosclerosis
  • Hypertension

Content & Storage

Content

1 x Cryopreserved ampule of AoSMC containing ≥ 500,000 cells/vial

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AoSMC – Human Aortic Smooth Muscle Cells

AoSMC – Human Aortic Smooth Muscle Cells

Human aortic smooth muscle cells (CC-2571) were grown in flasks and subsequently sub-cultured into 24-well plates, at 20k cells in Smooth muscle cell growth medium SmGM-2 medium (CC-3182). Cells were fixed and immunostained with 1:1500 dilution of anti-smooth muscle actin antibody (CBL171, Sigma), and nuclei were stained with Hoechst stain.

人主动脉平滑肌细胞 (CC-2571) 在烧瓶中生长，随后在平滑肌细胞生长培养基 SmGM-2 培养基 (CC-3182) 中以 20k 个细胞传代培养到 24 孔板中。 将细胞固定并用 1:1500 稀释的抗平滑肌肌动蛋白抗体 (CBL171, Sigma) 进行免疫染色，细胞核用 Hoechst 染料染色。

AoSMC – Human Aortic Smooth Muscle Cells

AoSMC – Human Aortic Smooth Muscle Cells

AoSMC – Human Aortic Smooth Muscle Cells

Phase contrast images of human aortic smooth muscle cells (CC-2571) cultured in SmGM-2 medium (CC-3182).

AoSMC – Human Aortic Smooth Muscle Cells
在 SmGM-2 培养基 (CC-3182) 中培养的人主动脉平滑肌细胞 (CC-2571) 的相差图像。