lonza,CC-2541,NHBE-不含维甲酸的人支气管/气管上皮细胞

Catalog #: CC-2541

Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells without Retinoic Acid containing ≥500,000 cells

Product Overview

Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza’s Normal Human Bronchial Epithelial Cells (NHBE) without retinoic acid are available. Certain lots with Small Airway epithelial cells (CC-2547) or normal human lung fibroblasts (CC-2512)  from matched donors are available.

Cryopreserved human bronchial epithelial cells without retinoic acid are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

正常和患病的支气管上皮(NHBE 和 DHBE)细胞是从肺分叉上方的气道上皮衬里分离出来的。 Lonza 提供不含维甲酸的正常人支气管上皮细胞 (NHBE)。 某些批次的小气道上皮细胞 (CC-2547) 或来自匹配供体的正常人肺成纤维细胞 (CC-2512) 可用。

不含维甲酸的冷冻保存的人支气管上皮细胞通过 15 次群体倍增得到保证。 细胞对支原体、细菌、酵母和真菌检测呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Select Applications with Lonza’s Human Bronchial Epithelial Cells –

  • A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza’s bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
  • Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.
  • Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

Content & Storage

Content

1 x Cryopreserved ampule containing ≥500,000 cells

Related Products
NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S

NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S

NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma for B-ALI™ Culture 00194911S
D-HBE-As – 患病的人支气管/气管上皮细胞 – B-ALI™ 培养的哮喘 00194911S

NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid

Data

NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid

Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media

Data Courtesy of Furukawa, et al. Lonza’s SAEC cells were co-cultured with primary normal mammary epithelial cells, breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza’s SAGM™ BulletKit™ Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of small airway epithelial cells. The image above shows phase contrast imaging with a fluorescent (red) overlay of MCF-7 cell line. MCF-7 cell lines survived as tight clusters in SAGM™ Media. When co-cultured with Lonza’s primary SAECs, MCF-7 cells demonstrated enhanced growth all the while staying clustered.

NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid
NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid

Co-culture Experiments with Lonza’s Bronchial Epithelial Cells and BEGM™ BulletKit™ Media

Furukawa, et al. demonstrates BEGM™ BulletKit™ is suitable for culturing Lonza’s NHBEs as well as to support co-cultures with lung epithelial cell line, BEAS-2B, and breast cancer cell lines, MCF-7 and MDA-MB-231. The breast cancer cell line showed differences in morphology and proliferation rates when cultured with primary NHBE cells vs. BEAS-2B cell line. The paper highlights the importance of using primary NHBE cells as a side-by-side control when conducting experiments with normal lung epithelial cell line such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines.

NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid
古川等人。 演示 BEGM™ BulletKit™ 适用于培养 Lonza 的 NHBE,并支持与肺上皮细胞系 BEAS-2B 和乳腺癌细胞系 MCF-7 和 MDA-MB-231 的共培养。 当与原代 NHBE 细胞与 BEAS-2B 细胞系一起培养时,乳腺癌细胞系在形态和增殖率方面表现出差异。 本文强调了在对正常肺上皮细胞系(如 BEAS-2B)进行实验时使用原代 NHBE 细胞作为并排对照的重要性,因为未转化的原代细胞可以验证或抵消使用细胞系生成的数据。