lonza,CC-2535,CC-2904,CC-2903,CC-2585,V5SP-5002,HAEC-人主动脉内皮细胞

Catalog #: CC-2535

Cryopreserved ampule of Human Aortic Endothelial Cells (HAEC) containing ≥ 500,000 cells

含有 ≥ 500,000 个细胞的人主动脉内皮细胞 (HAEC) 冻存安瓿

Product Overview

Cardiac Endothelial Cells include cells isolated from the aorta (HAEC), coronary artery (HCAEC), pulmonary artery (HPAEC), iliac artery (HIAEC), and microvascular cardiac cells (HMVEC-C).  Additional donor characteristics including hypertension, or smoking history may be available by contacting Scientific Support. Cardiac fibroblasts from matched donors are available for certain lots.

CloneticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.  Cryopreserved HAECs are shipped in third passage.

Cryopreserved CloneticsTM Human Aortic Endothelial Cells (HAEC) are guaranteed through 15 population doublings, express CD31/105, von Williebrand Factor VIII, and are positive for acetyated low density lipoprotein uptake.  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
心脏内皮细胞包括从主动脉 (HAEC)、冠状动脉 (HCAEC)、肺动脉 (HPAEC)、髂动脉 (HIAEC) 和微血管心脏细胞 (HMVEC-C) 分离的细胞。联系科学支持可以获取其他供体特征,包括高血压或吸烟史。来自匹配供体的心脏成纤维细胞可用于某些批次。

CloneticsTM 细胞、培养基和试剂一起经过质量测试,保证作为一个完整的细胞系统提供最佳性能。冷冻保存的 HAEC 在第三次运输中运输。

冷冻保存的 CloneticsTM 人主动脉内皮细胞 (HAEC) 通过 15 次群体倍增得到保证,表达 CD31/105、von Williebrand 因子 VIII,并且对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Benefits

  • Guaranteed through 15 population doublings
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate of Analysis is provided for each cell lot purchased

 

Content & Storage

Content

1 x Cryopreserved ampule HAEC containing ≥ 500,000 cells

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HAEC – Human Aortic Endothelial Cells

Data
HAEC – Human Aortic Endothelial Cells

HAEC – Human Aortic Endothelial Cells

Human aortic endothelial cells (CC-2535) were immuno histochemically stained with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1094 DyLight 594 (Vector Laboratories). Cells were also satined with CD31/PECAM-1 antibody (R&D Systems, BBA7) used at 1:100 (green). Secondary antibody used to detect was VECTDI2488 DyLight 488 (Vector Laboratories) Hoechst nuclear stain (blue).

用 1:200 使用的血管性血友病因子抗体 (Sigma, F3520) 对人主动脉内皮细胞 (CC-2535) 进行免疫组织化学染色。 使用的二抗是 VECTDI1094 DyLight 594 (Vector Laboratories)。 细胞也用 1:100(绿色)使用的 CD31/PECAM-1 抗体(R&D Systems,BBA7)饱和。 用于检测的二抗是 VECTDI2488 DyLight 488(Vector Laboratories)Hoechst 核染色剂(蓝色)。

HAEC – Human Aortic Endothelial Cells
HAEC – Human Aortic Endothelial Cells

HAEC – Human Aortic Endothelial Cells

Human aortic endothelial cells were immunohistochemically stained with VE Cadherin antibody (Santa Cruz,sc-28644) used at 1:200 dilution (red). Secondary antibody used was VECTDI1094 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst stain at 1:10,000 dilution.

 用 1:200 稀释度(红色)的 VE 钙粘蛋白抗体(Santa Cruz,sc-28644)对人主动脉内皮细胞进行免疫组织化学染色。 使用的二抗是 VECTDI1094 DyLight 594 (Vector Laboratories)。 细胞核以 1:10,000 稀释度用 Hoechst 染料染色。

lonza CC-7030,CC-2535,CC-2585,HMVEC-C-人心脏微血管内皮细胞

Catalog #: CC-7030

Cryopreserved ampule of Human Cardiac Microvascular Endothelial Cells (HMVEC-C) containing ≥ 500,000 cells

含有 ≥ 500,000 个细胞的人心脏微血管内皮细胞 (HMVEC-C) 冻存安瓿

Product Overview

Human Cardiac Microvascular Endothelial Cells (HMVEC-C) are isolated from normal and diabetic donors. Additional donor characteristics including hypertension, or smoking history may be available by contacting our Scientific Support Team.

CloneticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system. Cryopreserved Human Cardiac Microvascular Endothelial Cells are shipped in third passage.

Cryopreserved Human Cardiac Microvascular Endothelial Cells (CC-7030) are guaranteed through 10 population doublings, express CD31/105, von Williebrand Factor VIII, and are positive for acetyated low density lipoprotein uptake. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

人心脏微血管内皮细胞 (HMVEC-C) 是从正常和糖尿病供体中分离出来的。 联系我们的科学支持团队,可以获得其他捐赠者特征,包括高血压或吸烟史。

CloneticsTM 细胞、培养基和试剂一起经过质量测试,保证作为一个完整的细胞系统提供最佳性能。 冷冻保存的人心脏微血管内皮细胞在第三代运输。

冷冻保存的人类心脏微血管内皮细胞 (CC-7030) 保证通过 10 次群体倍增,表达 CD31/105、von Williebrand 因子 VIII,并且对乙酰化低密度脂蛋白摄取呈阳性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Benefits

  • Guaranteed through 15 population doublings
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

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