Structural Formula:
Labeling Procedure1. 将蛋白溶液在偶联缓冲液（10-20 mM MOPS、0.2 M NaCl、2 mM EDTA、5% 甘油、pH 8.0）中于 4ºC 透析过夜。 2. 透析后，将蛋白质浓度调整到 15-30 mM.3。 将 15 μl 20 mM FeBABE DMSO 溶液添加到 1 ml 蛋白质溶液中，并在 37ºC 下孵育 1 小时。 FeBABE 的最终浓度为 0.3 mM（比蛋白质过量 10-20 倍）。4。 在 4ºC 下将反应混合物在蛋白质储存缓冲液（10-20 mM Tris、0.1-0.2 M KCl、10 mM MgCl2、0.1 mM EDTA、50% 甘油、pH 7.6）中透析过夜。
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