标签：BE02-034E

lonza,lonza CC-5034,BE02-034E,CC-5002,ReagentPack™ 继代培养试剂

Catalog #: CC-5034

ReagentPackTM Subculture Reagents for primary cell subculturing

用于原代细胞继代培养的 ReagentPackTM Subculture Reagents

Product Overview

ReagentPackTM Subculture Kits have been designed specifically for the passaging of primary cell types. Each kit contains 100 mL each of: Trypsin/EDTA, Trypsin Neutralizing Solution, HEPES Buffered Saline

The ReagentPackTM Subculture Reagents kit is recommended for use with Lonza’s primary cells to ensure effective trypsinization and a successful cell passaging process.

ReagentPackTM Subculture Kits 专为原代细胞类型的传代而设计。 每个试剂盒均包含 100 mL：胰蛋白酶/EDTA、胰蛋白酶中和溶液、HEPES 缓冲盐水

建议将 ReagentPackTM Subculture Reagents 试剂盒用于 Lonza 的原代细胞，以确保有效的胰蛋白酶消化和成功的细胞传代过程。

Content & Storage

Content

1 x Bottle Trypsin/EDTA 100 mL
1 x Bottle Trypsin Neutralizing Solution 100 mL
1 x Bottle HEPES Buffered Saline Solution 100 mL

Instructions, SDS, CoA

 

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ReagentPack™ Subculture Reagents, 100 mL
TrypZeanTM  recombinant Trypsin EDTA (1x) BE02-034E

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ReagentPack™ Subculture Reagents, 100 mL
Trypsin Neutralizing Solution, 100 mL CC-5002

胰蛋白酶中和溶液，100 mL CC-5002

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Trypsin/EDTA Solution, 100 mL CC-5012

胰蛋白酶/EDTA 溶液，100 mL CC-5012

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HEPES Buffered Saline Solution – 100 ml CC-5022

HEPES 缓冲盐溶液 – 100 ml CC-5022

ReagentPack™ Subculture Reagents, 100 mL
HEPES Buffered Saline Solution, 500 mL CC-5024
HEPES 缓冲盐溶液，500 mL CC-5024

lonza,lonza CC-5012,BE02-034E,CC-5034,胰蛋白酶/EDTA 溶液

Catalog #: CC-5012
Trypsin/EDTA 0.025% solution for use in cell culture

Product Overview

Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension.  It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8.  After treatment, trypsin activity can be halted by adding a serum media mixture or a trypsin inhibitor (from soybean, for example) in serum-free systems.

Trypsin/EDTA is also a component of Lonza’s ReagentPackTM Subculture Reagents kit (CC-5034) which includes Trypsin Neutralizing reagent and HEPES buffer.

胰蛋白酶是一种透明、无色黄色、高活性的牛酶制剂，用于将细胞从培养容器表面去除成悬浮液。 它通常在 0.025% 的工作浓度下使用，在平衡盐溶液中制备，不含钙或镁，pH 为 7.6-7.8。 处理后，可以通过在无血清系统中添加血清培养基混合物或胰蛋白酶抑制剂（例如来自大豆）来停止胰蛋白酶活性。

胰蛋白酶/EDTA 也是 Lonza 的 ReagentPackTM Subculture Reagents 试剂盒 (CC-5034) 的一个组成部分，其中包括胰蛋白酶中和试剂和 HEPES 缓冲液。

Content & Storage

Content

1 x Bottle Trypsin/EDTA 100 mL

Related Products

Trypsin/EDTA Solution, 100 mL
TrypZeanTM  recombinant Trypsin EDTA (1x) BE02-034E

TrypZeanTM 重组胰蛋白酶 EDTA (1x) BE02-034E

Trypsin/EDTA Solution, 100 mL
ReagentPack™ Subculture Reagents, 100 mL CC-5034
ReagentPack™ 继代培养试剂，100 mL CC-5034