标签：atto Extraction kit

WSE-7430 EzPBS,atto代理,atto Extraction kit

WSE-7430 EzPBS

Purpose and Application

  • EzPBS(-) is Phosphate buffered saline for cellular experiments
  • Available for cellular experiments
  • This is the most famous PBS which is 10x concentrated reagent
  • Available for Western blotting, ELISA, immunohistochemical staining and so on

Features

  • General phosphate buffered saline (Phosphate buffer, NaCl)
  • 10x Dilute with distilled water
  • Sterilized, DNase free
  • NOT include Calcium and Magnesium
  • Economical

Specifications

WSE-7430 EzPBS
Major Components Phosphate buffer pH 7.4, Saline 10X concentrated solution
(Final concentration 1.76 mM KH₂PO₄, 10 mM Na₂HPO₄, 2.7 mM KCl, 137 mM NaCl)
Package Solution 1 L / bottle
Preparation 10X dilute with distilled water
Applicable amount 10 L
Storage 1 year at room temperature

Ordering Information

Code No. Description Unit
2332380 WSE-7430 EzPBS 1 pk

目的和应用

EzPBS(-)是用于细胞实验的磷酸盐缓冲盐水
可用于细胞实验
这是最著名的PBS是10倍浓缩试剂
可用于Western blotting、ELISA、免疫组化染色等
特性

普通磷酸盐缓冲盐水(磷酸盐缓冲液，NaCl)
10x用蒸馏水稀释
消毒,DNase免费
不含钙和镁
经济

WSE-7423 EzBactYeastCrusher,atto代理,atto Extraction kit

WSE-7423 EzBactYeastCrusher

Purpose and Application

  • Protein extracting from Yeast & Bacteria for various experiments
  • Immunoprecipitation
  • Enzyme activity experiments
  • Electrophoresis
  • Western blotting etc.

Features

  • Simple operation
    (Mix reagent with bacterial cell, and then leave it→Centrifuge→Collect the supernatant)
  • Complete to prepare within 30 minutes
  • Glass beads, Homogenizer, Ultrasonic crushing apparatus are unnecessary
  • Absence of enzyme inhibition
  • Attached with DNase, Protease inhibitor
  • NOT include Amine
  • Absence of inhibition of column purification (His, GST etc.)

Specifications

WSE-7423 EzBactYeastCrusher
Package Yeast PreLysis buffer: 100 mL
BactYeastLysis buffer: 100 mL
Protease inhibitor (100X): 1 mL
DNase (100X): 1 mL
Applicable amount 100 samples, 50~100 mg/sample
Storage Yeast PreLysis buffer, BactYeastLysis buffer: 1 year at room temperature
Protease inhibitor, DNase: 1 year at -20℃

Ordering Information

Code No. Description Unit
2332339 WSE-7423 EzBactYeastCrusher 1 pk

目的和应用

从酵母和细菌中提取蛋白质用于各种实验
免疫沉淀反应
酶活性实验
电泳
西方墨点法等。
特性

简单的操作
(试剂与细菌细胞混合离开→离心→收集上清)
在30分钟内完成准备工作
不需要玻璃微珠、均质机、超声波破碎设备
无酶抑制
附DNase，蛋白酶抑制剂
不包括胺
柱纯化(His, GST等)无抑制作用

WSE-7422 EzSubcell Fraction,atto代理,atto Extraction kit

WSE-7422 EzSubcell Fraction

Purpose and Application

  • Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Bioactivity/Protein purification/ Chromatin immunoprecipitation assay/ Enzyme activity/ etc.

Features

  • Sample preparation for electrophoresis, immunoprecipitation, ELISA, chromatin immunoprecipitation assay, enzyme activity & bioactivity experiments, etc.
  • Simple preparation of organella fractionation in high reproducibility and purity.
  • Additionally required material is only cooled centrifuge( to 10,000 x g).
  • Process for all fractionation is completed in 2 hours.
  • Detergent or non-detergent method is available.

Specifications

WSE-7422 EzSubcell Fraction
Components Extraction buffer: HEPES, detergent
Protease Inhibitor (100X): Aprotinin, Pepstatin A, Leupeptin
Applicable amounts 50 samples, up to 2 x 10⁷ cell/sample
Storage 1 year
Package Fraction buffer 1: 50 mL
Fraction buffer 2: 50 mL
RIPA Lysis buffer: 20 mL
Detergent mix (50X): 1 mL
Protease Inhibitor (100X): 0.7 mL

Ordering Information

Code No. Description Unit
2332338 WSE-7422 EzSubcell Fraction 1 pk

目的和应用

电泳/ Western blotting/免疫沉淀/ ELISA/生物活性/蛋白纯化/染色质免疫沉淀测定/酶活性/等
特性

样品制备电泳，免疫沉淀，ELISA，染色质免疫沉淀，酶活性及生物活性实验等。
细胞器分离制备简单，重复性高，纯度高。
此外，所需材料只需冷却离心机(至10,000 x g)。
所有分馏过程在2小时内完成。
可用洗涤剂或非洗涤剂方法。

WSE-7421 EzSubcell Extract,atto代理,atto Extraction kit

WSE-7421 EzSubcell Extract

Purpose and Application

  • Electrophoresis/ Western blotting/ ImmunoprecipitationELISA/ chromatin immunoprecipitation assay/ Enzyme activity/ etc.

Features

  • Sample preparation for electrophoresis, immunoprecipitation, ELISA, chromatin immunoprecipitation assay, enzyme activity experiments, etc.
  • Simple operation by centrifuging after the addition of four extraction buffers step wisely.
  • Additionally required material is only cooled centrifuge( to 10,000 x g).
  • The process for all fractionation is completed in 3 hours. Both Fractionated cells from mammalian tissue and frozen cell are available.

Specifications

WSE-7421 EzSubcell Extract
Components Extraction buffer: HEPES, detergent
Protease Inhibitor (100X): Aprotinin, Pepstatin A, Leupeptin
Dnase I
Applicable amounts 50 samples, up to 2 x 10⁷ cell/sample
Storage 1 year
Package Extraction buffer 1: 50 mL
Extraction buffer 2: 50 mL
Extraction buffer 3: 25 mL
Extraction buffer 4: 25 mL
Protease Inhibitor (100X): 1.25 mL
Dnase I: 0.25 mL

Ordering Information

Code No. Description Unit
2332337 WSE-7421 EzSubcell Extract 1 pk
 目的和应用

电泳/ Western blotting/免疫沉淀elisa /染色质免疫沉淀测定/酶活性等
特性

样品制备电泳、免疫沉淀、ELISA、染色质免疫沉淀试验、酶活性实验等。
操作简单，离心后加入四种提取缓冲液步骤明智。
此外，所需材料只需冷却离心机(至10,000 x g)。
所有的分馏过程在3小时内完成。从哺乳动物组织分离的细胞和冷冻细胞都是可用的。

WSE-7420 EzRIPA Lysis kit,atto代理,atto Extraction kit

WSE-7420 EzRIPA Lysis kit

WSE-7420 EzRIPA Lysis kit,atto代理,atto Extraction kit

目的和应用

全细胞提取准备以下应用
电泳/ Western blotting/免疫沉淀/ ELISA/酶活/等
特性

样品制备电泳、免疫沉淀、ELISA、酶活实验等。
标准组包括蛋白酶抑制剂和磷酸酶抑制剂。
由于EzRIPA裂解试剂盒是基于HEPES的无胺缓冲液，因此制备的样品可以直接进行标记实验。

Purpose and Application

  • Whole cell extract preparation for the following applications
     Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Enzyme activity/ etc.

Features

  • Sample preparation for electrophoresis, immunoprecipitation, ELISA, enzyme activity experiments, etc.
  • Standard set includes Protease inhibitor and Phosphatase inhibitor.
  • Since EzRIPA Lysis kit is amine-free buffer that based on HEPES buffer, labeling experiments of prepared sample can be applied directly.

Specifications

WSE-7420 EzRIPA Lysis kit
Applicable amount 100 samples, up to 2 x 10⁷ cell/sample
Storage 1 year
Package – EzRIPA Lysis buffer: 100 mL
(20 mM HEPES pH 7.5, 1% IGEPAL® CA-630*, 0.1% SDS, 0.5% Deoxycholic acid, 150 mM Sodium Chloride)
– Protease Inhibitor (100x): 1 mL
(Aprotinin, Pepstatin A, Leupeptin)
– Phosphatase Inhibitor (100X): 10 mL
(Sodium fluoride, Sodium orthovanadate (V), β-sodium glycerophosphate)
* IGEPAL® CA-630 is the same as NonidetP-40, and a trade mark registration of RhonePoulenc AG Co.

Ordering Information

Code No. Description Unit
2332336 WSE-7420 EzRIPA Lysis kit 1 pk