Staining Procedure1.Prepare 10-50 μM AO solution with PBS or an appropriate buffer.a)2.Add AO solution with 1/10 of the volume of cell culture medium to the cell culture.b)3.Incubate the cell at 37ºC for 10-20 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells under a fluorescence microscope with 500 nm excitation and 530 nm emission filters.
a) Since AO may be carcinogenic, extreme care is necessary during handling.b) You may replace the culture medium with 1/10 concentration of AO buffer solution.
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