Purpose and Application
- Extraction of Protein and PolyPeptide
- Sample buffer for SDS-PAGE
Features
- Use of dithiothreitol (DTT) as a reducing reagent
- Reduction reaction more secure than 2-ME
- Less likely to produce false bands than 2-ME
- No smell like 2-ME (2-mercaptoethanol)
- Low running cost and simple preparation procedure
Specifications
| AE-1430 EzApply | |
|---|---|
| Major components | Tris-HCl buffer, 2% SDS, 20% sucrose, BPB, and 100 mM DTT (final concentrations: about halves of the above figures) |
| Package | Solution/30 mL bottle (EzApply solution) Solution/5 mL bottle x 5 bottles (DTT) |
| Preparation | EzApply solution is added to DTT bottles and mixed with sample at a ratio of 1:1 |
| Applicable amount | 5 mL x 5 |
| Storage | 6 months at -20℃ (before dissolved in DTT) 1 week after dissolved in DTT |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332330 | AE-1430 EzApply | 1 pk |
目的和应用
蛋白质和多肽的提取
用于SDS-PAGE的示例缓冲区
特性
使用二硫苏糖醇(DTT)作为还原剂
还原反应比2-ME更安全
比2-ME更不容易产生假带
没有2-ME(2-巯基乙醇)的味道
运行成本低，制备过程简单