ABD-F 具有苯并呋喃部分，可通过与巯基反应产生高荧光化合物。 衍生化合物的激发和发射波长分别为 389 nm 和 513 nm。 ABD-F的反应速度比SBD-F快30倍。 在 50ºC、pH 8 的水性条件下，ABD-F 与硫醇化合物的反应在 5 分钟内完成。但是，ABD-F 在这些条件下不与丙氨酸、脯氨酸或胱氨酸反应。 在 pH 2 时可以观察到其最大荧光强度。在反相 HPLC 分析中，可以单独检测预标记的 ABD-硫醇化合物。 检测限 (S/N=3) 为半胱氨酸每次注射 0.6 pmol，谷胱甘肽每次注射 0.4 pmol，N-乙酰半胱氨酸每次注射 1.9 pmol，半胱胺每次注射 0.5 pmol。
ABD Labeling Protocol1. To prepare sample solution, mix or dissolve a sample with 100 mM borate buffer, pH 8.0 containing 2 mM EDTA.2. Mix 500 μl of the sample solution and 500 μl of 1 mM ABD-F/100 mM borate buffer in a reaction vial.3. Heat the vial at 50ºC for 5 minutes and cool it on an ice bath.4. Add 300 μl of 100 mM HCl aqueous solution to the reaction mixture.5. Use this mixture for HPLC analysis to determine ABD-labeled compounds; excitation: 389 nm, emission: 513 nm.
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