标签：00192627

Product Overview

Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult cells. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Normal Human Dermal fibroblasts from matched donors are available for certain lots.

冷冻保存的正常人表皮角质形成细胞通过 18 次成体细胞的群体倍增得到保证。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。 来自匹配供体的正常人真皮成纤维细胞可用于某些批次。

Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 00192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 00192627) and KGM^TM-Gold BulletKit^TM Growth Media (catalog no. 00192060)

References with Lonza’s Keratinocytes:

• Protective barrier: Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M,  Mlitz V,  Gruber F,  Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E.  Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47. 

• Skin model: Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207. 

• Cancer: Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS.  EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71.

• Human Papillomaviruses (HPV) mediated skin cancer response: An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients.  The present study utilized pooled keratinocytes cultured in KGM^TM-Gold Medium from several healthy donors. The data showed that betaPV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165.