Dojindo,谷氨酸检测试剂盒-WST/100/G269,Glutamate Assay-Kit-WST

谷氨酸是一种重要的神经递质,有助于蛋白质和谷胱甘肽的生物合成。 过量的谷氨酸被认为是导致神经退行性疾病的原因,包括阿尔茨海默病。 最近的一项研究表明,抑制负责胱氨酸摄取和谷氨酸释放的胱氨酸/谷氨酸逆向转运蛋白 (xCT) 会诱导一种称为“铁死亡”的铁依赖性细胞死亡。 因此,近年来也开展了针对 xCT 的癌症研究。我们的谷氨酸检测试剂盒-WST 旨在量化谷氨酸作为代谢物。 该试剂盒允许您通过 WST 还原反应对培养基中的谷氨酸或细胞内谷氨酸进行定量。 可量化的最低谷氨酸浓度为 5 μmol/l。 该试剂盒可与 96 孔微孔板一起使用,从而可以分析多个样本。

PrincipleThe kit is used to detect Glutamate in cell culture medium by measuring the absorbance of WST formazan produced according to quantities of glutamate. The kit includes a “Glutamate Standard” which can be used to quantify the concentration of lactate found within samples by creating standard curve.


Simple Assay ProcedureProcedures are so easy that you simply incubate the plate after the addition of culture supernatant or tissue/cell lysates prior to adding a reagent.


Standard CurveStandard curve can be prepared by using Glutamate Standard included in the kit. The concentration of Glutamate can be measured. The concentration of lactate can be evaluated by diluting the samples if the concentration is over 0.5 mmol/l.


Example of Measurements of Glutamine/Glutamate level


Example of Measurements of Glutamine/Glutamate level in Ferroptosis researchIt is known that an iron-dependent cell death called “ferroptosis” is induced when xCT is inhibited by elastin treatment. The amounts of glutamate release and intracellular glutathione were measured by using the elastin-treated A549 cells. As a result, the amount of glutamate release was decreased in the elastin-treated A549 cells, and the amount of intracellular glutathione was decreased by inhibiting cystine uptake.

How many samples can I measure?
The number of samples that can be recorded when the standard curve and sample is measured in triplicates.100 TestsSample 24 samples*(measured in triplicate – See picture below)

A plate layout for Glutamate standard solution and sample

Can I quantify D-Glutamate levels?
No, the kit is used for the quantification of L-Glutamate levels.
Can I measure samples that contain a reducing agent?
You can’t measure an accurate Glutamine level because WST dye interferes with the reducing agent. If you add test material which possesses reducing properties, please prepare a few wells that only contain the medium without cells and only test material for background control.
Our samples did not change in color, are there any reasons for this?
Glutamine level contained in the sample may be lower than the detection limit (5 µmol/l) that can be measured using this kit. If the lactate level is below 5 µmol/l.If you dilute the sample, lactate level contained in the diluted sample may be lower than 5 µmol/l.Please lower the dilution ratio and adjust the concentration of the sample over the detection limit that was measured using this kit.