标签：葡萄糖分析试剂盒WST/200/G264

Dojindo,葡萄糖分析试剂盒WST/200/G264

原理本试剂盒可以通过测量有色WST甲臜染料的吸光度来检测细胞培养基中的葡萄糖或细胞内的葡萄糖。 吸光度取决于样品中存在的葡萄糖量。该试剂盒包含可用于创建标准曲线的葡萄糖标准溶液。 这允许对样品中存在的葡萄糖水平进行定量。

程序 程序非常简单，您只需在添加培养上清液或组织/细胞裂解物后，在添加试剂之前孵育板即可。

标准曲线的制备 样品中的葡萄糖水平可以通过使用该试剂盒中包含的葡萄糖标准建立的校准曲线来测量。 如果葡萄糖水平大于或等于 0.5 mmol/L，则必须在测量前稀释样品。

结合 Lactate Assay Kit 测量葡萄糖水平通过使用 Glucose Assay Kit-WST 和 Lactate Assay Kit-WST，我们成功测量了将蛋白转运抑制剂根皮素添加到 Jurkat 细胞中时的代谢活性变化。

Precautions when using this kit.

Cell counts may need to be normalized. When cells are analyzed in a microplate, the results obtained may sometimes differ depending on cell numbers per well.In such cases, normalization of the measured values obtained from cell counting and total protein will be necessary.In this kit, cell numbers can be easily measured by the fluorescence intensity induced by a reagent added to cell culture medium for staining nuclei.

I do not have a 450 nm filter. What other filters can I use?
You can use filters with an absorbance between 450 nm and 490 nm. However, the absorbance value can become lower if the sample is not measured at 450 nm.
Can I quantify L-Glucose levels?
No, the kit is used for the quantification of β-D-Glucose levels.
How many samples can I measure?
* The number of samples that can be recorded when the standard curve and sample is measured in triplicates.*A plate layout for Glucose standard solution and sample