-Bacstain- Bacterial Viability Detection Kit – CFDA/PI uses esterase activity of bacteria and bacterial membrane damage as an index. CFDA is hydrolyzed to carboxyfluorescein―which is fluorescent―by intracellular esterase. PI is a parallel intercalator into the DNA double helix that stains nucleic acids; it passes only through damaged bacterial membranes. Thus, this kit can be used to measure the ratio of bacteria with esterase activity to membrane-damaged bacteria on the analysis of the fluorescent images from each stain.
Simple Procedure
Double-staining of Staphylococcus aureus (a Gram-positive bacterium)
Quantitative analysis
-Bacstain- Bacterial Viability Detection Kit – DAPI/PI (BS08) and –CTC/DAPI (BS09) were used to evaluate the effect of benzalkonium chloride on S. aureus (Gram-positive bacteria) by fluorescent imaging. The images were quantified by ImageJ and show the correlation with drug concentration.The results showed that the effects of benzalkonium chloride on the respiratory activity and membrane damage of S. aureus varied greatly depending on the indicator detected.The evaluation of multiple indicators will contribute to improving the reliability of drug efficacy measurements by providing a multidimensional view of the activity of the bacterium that would be missed by a single indicator.
Related Product Information
| Product | Size | Product Code |
| -Bacstain- CFDA solution | 100 assays | BS03 |
| -Bacstain- DAPI solution | 100 assays | BS04 |
| -Bacstain- AO solution | 100 assays | BS05 |
| -Bacstain- PI solution | 100 assays | BS07 |
| -Bacstain- CTC Rapid Staining Kit (for Flow cytometry) | 100 assays | BS01 |
| -Bacstain- CTC Rapid Staining Kit (for Microscopy) | 100 assays | BS02 |