Reagents > Histone H3 Rabbit Monoclonal Antibody for Normalization
The Histone H3 primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting target proteins in nuclear extracts.
The expression of Histone H3, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, Histone H3 primary antibodies can be used for the detection of Histone H3 when performing multiplex Western blot detection.
Detect Histone H3 Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
组蛋白 H3 一抗可用作标准化的内部上样对照，在检测核提取物中的靶蛋白时特别有效。
应验证组蛋白 H3 或任何管家蛋白 (HKP) 的表达，以确保其表达在实验条件下不会改变。
经验证后，在进行多重蛋白质印迹检测时，组蛋白 H3 一抗可用于检测组蛋白 H3。
使用 IRDye® 山羊抗兔或 IRDye 驴抗兔二抗检测组蛋白 H3 兔单克隆抗体。
Other options for housekeeping protein normalization
Reactivity and Specificity
Histone H3 antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | Histone H3 Rabbit Monoclonal Antibody (P/N 926-42219) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 17 kDa |
| Isotype | Rabbit IgG |
| Specificity/Sensitivity | Detects endogenous levels of total Histone H3 protein (including isoforms H3.1, H3.2, and H3.3) and Histone H3 variant CENP-A. Does not cross-react with other core histones. May cross-react with bovine, chicken, D. melanogaster, hamster, xenopus, and zebrafish. |
| Immunogen | A synthetic peptide that corresponds to the carboxy terminus of the human histone H3 protein |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)
蛋白质印迹 (WB)、免疫组织化学 (IHC)、免疫荧光 (IF)、流式细胞术 (F) |
RRID
- P/N 926-42219: RRID AB_2814902
Detection of Histone H3 Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and Cos7 Lysates
