Fig. 1 Chemical Reaction with sulfane sulfer
Fig. 2 Fluorescence spectrum (Em: 482nm/Ex: 515nm)
Chemical species containing sulfane sulfurs:
Fig. 3 The formation of sulfane sulfer in vivo changes due to reduction/oxidation and translation
1. W. Chen, C. Liu, B. Peng, Y. Zhao, A. Pacheco, and M. Xian,“New fluorescent probe for sulfane sulfurs and the application in bioimaging“, Chem. Sci., 2013, 4, 2892.2. T. Ida, T. Sawa, H. Ihara. Y. Tsuchiya, Y. Watanabe, Y. Kumagai, M. Suematsu, H. Motohashi, S. Fujii, T, Matsunaga, M. Yamamoto, K. Ono, N. O. Devarie-Baez, M. Xian, J. M Fukuto, and T. Akaike, “Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling”, Proc Natl Acad Sci U S A., 2014, 111, 7606.3. E. Marutani, M. Sakaguchi, W. Chen, K. Sasakura, J. Liu, M. Xian, K. Hanaoka, T. Nagano, and F. Ichinose, “Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists mediated by intracellular sulfane sulfur”, Med. Chem. Commun., 2014, 5, 1577.4. M. Sakaguchi, E. Marutani, H-S. Shin, W. Chen, K. Hanaoka, M. Xian, and F. Ichinose, “Sodium Thiosulfate Attenuates Acute Lung Injury in Mice”, Anesthesiology. 2014,121, 1248.
Cell Imaging:
Left: Control cellsRight: Treatment of 100 μmol/l Na2S3
Cell: CHO cellExposure time: 1,000 msc