Accessories > MPX™ Blotting System for Multiple-Target Western Blots,用于多靶点蛋白质印迹的 MPX™ 印迹系统
The MPX Blotting System is ideal for any multiple-target Western blot application. This system increases Western blot throughput, maximizes efficiency, and reduces relative overall cost.
MPX 印迹系统是任何多靶点蛋白质印迹应用的理想选择。 该系统提高了蛋白质印迹通量，最大限度地提高了效率，并降低了相对总成本。
Screen Samples or Optimize Blotting Conditions with the MPX Blotting System
Screen multiple samples and multiple targets all on the same blot without cutting, stripping, or cross-contamination.
Use the MPX Blotter for a variety of assays, such as primary and secondary antibody screening, monoclonal antibody screening, blocking buffer optimization as well as multi-target probing of 1 – 4 samples on a single membrane. Most common Western procedures that generate a PVDF or nitrocellulose blot of 7 cm x 8.5 cm can be adapted to the MPX format.
在同一个印迹上筛选多个样品和多个靶标，无需切割、剥离或交叉污染。
使用 MPX Blotter 进行各种检测，例如一抗和二抗筛选、单克隆抗体筛选、封闭缓冲液优化以及单膜上 1-4 个样本的多靶点探测。 产生 7 cm x 8.5 cm 的 PVDF 或硝酸纤维素印迹的最常见的西方程序可以适应 MPX 格式。
Simple, Convenient Workflow
Channel ports are conveniently spaced, staggered, and beveled to create a simple workflow for using both standard and multi-channel pipettes. The low volume channel ports accommodate a maximum volume of 160 µL, thus conserving precious and costly antibody. The nesting dovetail joint design allows multiple MPX units to be linked together for easy-to-handle multiple-blot processing.
Screen Up To 48 Targets on One Blot
Processing is as simple as clamping the blot into the MPX, creating up to 24 independent channels. The range of usable channels per sample is relative to comb-size – from 24 on a single sample to four on a four-sample blot.
Using LI-COR’s selection of IRDye® dye-conjugated secondary antibodies on the Odyssey Imagers provides a maximum of 48-targets on a single membrane— two per channel—and the option for quantitative analysis. Other detection methods, such as standard chemiluminescence and colorimetric, can provide a maximum 24 targets.
MPX Blotting System Specifications
- Overall Size: 15.5 cm x 8.5 cm x 3.5 cm (L x W x H)
- Membrane Size: 7 cm x 8.5 cm (H x L)
- Channel Dimensions: 50 mm x 1.5 mm x 1.5 mm (L x W x H)
- Number of Channels: 24
- Total Volume per Channel Including Port: 160 µL
MPX Western Blot with One Sample and Multiple Targets
