标签：洗脱

产品名称	RNA清洁珠
英文名称	RNA Clean Beads
运输条件	冰袋运输

产品描述

该磁珠适用于RNA片段快速分选和回收。磁珠经过磁性分离和乙醇清洗，低盐洗脱缓冲液或无核酸酶水洗脱的高纯度RNA片段不含有核苷酸、引物、酶和盐等污染物，可被直接用于下游应用，例如：测序、杂交、RT-PCR反应等，并可应用于手动或自动液体操作设备。

产品特点

1、回收率可达80%以上；

2、操作快速简单，20min即可完成整个操作流程，无需离心。

使用建议

1.  RNA回收效率低或者没有？

    1）RNA发生降解。操作过程要严格的确保无RNase的污染。

    2）样本质量低。

    3）洗脱效率低。80%乙醇需现用现配，放置时间过久，可能由于乙醇挥发，造成浓度降低，影响洗脱效果；

    4）洗脱溶液孵育时间过短。磁珠应于洗脱液中按规定时间孵育，常规为5 min；

    5）磁珠过度干燥。切勿室温干燥磁珠大于10 min，过度干燥将降低洗脱效率。

2. 纯化RNA中有磁珠残留？

    1）磁珠分离时间过短或磁力器磁力较弱。增加分离时间或选用磁力强的磁力器，确保磁珠被磁力器全部吸附；

    2）洗脱步骤中，吸取上清速度过快。缓慢吸取上清，注意切勿吸取磁珠。

3. 如何检测回收后RNA的纯度？

    可以通过测定A260/A280的比值来评估。

保存条件

4℃保存。

Product Description

The magnetic beads are suitable for rapid sorting and recovery of RNA fragments. Magnetic beads are magnetically separated and washed with ethanol. The high-purity RNA fragments eluted with low-salt elution buffer or nuclease-free water do not contain contaminants such as nucleotides, primers, enzymes, and salts, and can be directly used in downstream applications. For example: sequencing, hybridization, RT-PCR reaction, etc., and can be applied to manual or automatic liquid handling equipment.

Features

1. The recovery rate can reach more than 80%;

2. The operation is fast and simple, and the entire operation process can be completed in 20 minutes without centrifugation.

Recommendations

1. Inefficient or no RNA recovery?

1) RNA is degraded. The operation process should be strictly ensured that there is no RNase contamination.

2) The sample quality is low.

3) The elution efficiency is low. 80% ethanol needs to be used and prepared immediately. If it is stored for a long time, the concentration may decrease due to the volatilization of ethanol, which will affect the elution effect;

4) The incubation time of the elution solution is too short. The magnetic beads should be incubated in the eluent for a specified time, usually 5 min;

5) Magnetic beads are over-dried. Do not dry the beads at room temperature for more than 10 minutes, as excessive drying will reduce the elution efficiency.

2. Are there magnetic beads remaining in the purified RNA?

1) The separation time of the magnetic beads is too short or the magnetic force of the magnetizer is weak. Increase the separation time or choose a magnetizer with strong magnetic force to ensure that the magnetic beads are all adsorbed by the magnetizer;

2) During the elution step, the supernatant was aspirated too fast. Aspirate the supernatant slowly, taking care not to aspirate the beads.

3. How to check the purity of recovered RNA?

It can be assessed by measuring the ratio of A260/A280.

Storage Conditions

Store at 4°C.

储存温度	2-8°C储存
品牌	金畔生物