lonza,PT-3003,人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM

Culture system containing hMSC Chondrogenic Basal Medium (PT-3925) and hMSC Chondrogenic SingleQuotsTM Kit Supplement (PT-4121) required for hMSC chondrogenic differentiation
lonza,PT-3003,人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM

Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including chondrocytes. Lonza’s hMSC Chondrogenic Differentiation BulletKitTM Medium is a serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin.

干细胞具有分化成包括软骨细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 软骨分化 BulletKitTM 培养基是一种含有血清的培养基,旨在通过添加 TGF-β3 诱导人骨髓来源的间充质干细胞在短短 14 天内向成熟、功能活跃的软骨细胞分化。 然后可以通过多种方法验证分化,包括用爱新蓝或番红染色。

Partial List of Cells Differentiated with hMSC Chondrogenic Differentiation BulletKitTM Medium*:

  • Human bone marrow derived stem cells
  • Human umbilical cord blood derived stem cells
  • Human umbilical cord matrix derived stem cells
  • C57BL/6 mice bone marrow derived stem cells
  • human adipose derived stem cells
  • Human hamstring tendon derived stem cells

 

添加 TGF-β3(单独出售)的 hMSC 软骨分化 BulletKitTM 培养基提供了一种分化培养基,可保证诱导骨髓来源的 hMSC 分化为成熟的功能活跃的软骨细胞;每瓶 hMSC 软骨分化 BulletKitTM 培养基都含有足够的分化培养基,可将至少 200 万个骨髓来源的 hMSC [大约 4 个完整的 24 孔板] 完全分化为成熟的功能活跃的软骨细胞。

hMSC 软骨分化培养基以 BulletKitTM 培养基(目录号 PT-3003)的形式提供,其中包括基础培养基和必要的补充剂(添加 TGF-β3 [单独出售,目录号 PT-4124]),用于骨髓来源的 hMSCs 的成软骨分化。基础培养基(Differentiation Basal Medium – Chondrogenic;目录号 PT-3925)和必要的补充剂(HMSC SQ 试剂盒 – 软骨;目录号 PT-4121 和用于 HMSC Chondro diff 的 TGF Beta 3;目录号 PT-4124)也可以单独购买。

只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助,请联系 Lonza Scientific Support。

*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用,因此只能保证这种培养基与这些细胞一起成功使用。

Content & Storage

Content

1 x hMSC Chondrogenic Basal Medium (PT-3925)

1 x hMSC Chondrogenic SingleQuot Kit (PT-4121)

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