标签：人外周血单个核细胞 (hPBMC)

Product Overview

Lonza is your ideal partner when you are looking to buy PBMC. Our Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood by apheresis and density gradient separation. They are guaranteed to contain ≥50 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Ability to Screen Different Donors

Sample representation in your experiment matters. We maintain an inventory of different donors in an effort to satisfy your sample representation requirements. You can choose a lot based on donor demographic characteristics, and screen multiple lots to find the lot(s) that work best for you. In addition, we can request repeat donors if you need additional cells from a particular donor.

Rich Source of Immune Cells

Peripheral blood mononuclear cells (PBMC) are a mixed population of single nucleus cells. They can be further purified to isolate individual cell types such as natural killer cells, T cells, and B cells. In addition, PMBCs are a rich source of monocytes, which can be directed to differentiate into either macrophages or dendritic cells through culture with various cytokines. Supported by an Optimized Media System LGM-3^TM Lymphocyte Growth Medium was specifically designed for support and maintenance of lymphocytes and is optimized for serum-free culturing (additional cytokines may be necessary depending on application) Alternative media containing serum may also be acceptable.

Please contact Scientific Support for serum containing media recommendations. Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

当您想购买 PBMC 时，Lonza 是您理想的合作伙伴。我们的外周血单个核细胞 (PBMC) 通过单采和密度梯度分离从外周血中分离出来。它们保证每个安瓿含有≥5000万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

筛选不同捐赠者的能力

实验中的样本表示很重要。我们保留一份不同捐赠者的清单，以满足您的样本代表要求。您可以根据捐赠者的人口统计特征选择批次，并筛选多个批次以找到最适合您的批次。此外，如果您需要来自特定捐赠者的额外细胞，我们可以请求重复捐赠者。

丰富的免疫细胞来源

外周血单个核细胞 (PBMC) 是单核细胞的混合群体。它们可以进一步纯化以分离单个细胞类型，例如自然杀伤细胞、T 细胞和 B 细胞。此外，PMBC 是单核细胞的丰富来源，可通过与各种细胞因子一起培养，定向分化为巨噬细胞或树突状细胞。由优化的培养基系统支持 LGM-3TM 淋巴细胞生长培养基专为支持和维持淋巴细胞而设计，并针对无血清培养进行了优化（根据应用，可能需要额外的细胞因子） 也可以接受含有血清的替代培养基。

有关含血清培养基的建议，请联系科学支持。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。