Cryopreserved ampule of Human Uterine Microvascular Endothelial Cells (UtMVEC-Myo) containing ≥500,000 cells
Product Overview
各种 NucleofectorTM 试剂盒和相应的 AmaxaTM 优化方案可用于使用不同的 NucleofectionTM 平台转染 NCTC 克隆 929 细胞。
- For transfection of NCTC clone 929 in the 4D-NucleofectorTM System and 96-well ShuttleTM System we recommend using our Cell Line Optimization Kits and the respective optimization protocols.
- Optimal kits for the NucleofectorTM II Device are the Cell Line NucleofectorTM Kits V used in combination with a cell-type specific protocol.
NucleofectionTM Performance
- Transfection efficiency up to 67%
- Viability up to 91%
Example for NucleofectionTM of NCTC clone 929 cells
使用 pmaxGFPTM 载体通过 NucleofectionTM 转染 NCTC 克隆 929 细胞。 在 NucleofectionTM 后培养细胞 10 分钟。 NucleofectionTM 后 48 小时,通过光 (A) 和荧光显微镜 (B) 分析细胞。
对于 4D-NucleofectorTM 试剂盒,“L”和“S”表示 NucleocuvetteTM Vessel 格式。 L = 100 µl 单 NucleocuvetteTM,S = 16 孔 NucleocuevtteTM Strip。