2022 3月 26 | BETHYL抗体金畔生物官网

lonza 3W-602,原代细胞和干细胞,新鲜人幼稚 CD19+/IgD+ B 细胞

Catalog #: 3W-602

Human Naïve CD19+/IgD+ B cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 1 million cells, fresh

Product Overview

使用免疫磁性分离系统从 MNC 中阴性选择白细胞分离的 CD19+/IgD+ B 细胞以消耗非初始 B 细胞。细胞在离心管中运输。使用 FACS 测定法确定细胞纯度。使用 AO/PI 确定计数和活力。按照 IRB 协议从健康供体收集细胞。由 AllCells® 制造新鲜的人源产品需支付额外运费

Applications

Generating antibody libraries Co-culture with
T helper cells or cytotoxic lymphocytes (CTL)
B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
Antigen presenting cell (APC) signal transduction and antigen presentation

Content & Storage

Content

1 vial containing ≥ 1 million fresh cells

lonza,原代细胞和干细胞,Human Naïve B Cells – CD19+/IgD+

Catalog #: 4W-602

Human CD19+/IgD+ B cells isolated from peripheral blood using negative immunomagnetic selection, cryopreserved, ≥1 million cells

Overview

Product Overview

Leukapheresed CD19+/IgD+ B cells are negatively selected from mononucleated cells using immunomagnetic isolation system to deplete non-naive B cells. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Manufactured by AllCells®

Applications

Generating antibody libraries
Co-culture with T helper cells or cytotoxic lymphocytes (CTL)
B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
Antigen presenting cell (APC) signal transduction and antigen presentation

Content & Storage

Content

1 vial containing ≥ 1 million cryopreserved cells

Instructions, SDS, CoA

Instructions

1 of

Safety Data Sheets (SDS)

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SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Educational Material

Brochures, White Papers etc.

1 of

FAQ & Citations

lonza 00227730,原代细胞和干细胞,Por-CASMC猪冠状动脉平滑肌细胞

Catalog #: 00227730

限量版猪冠状动脉平滑肌细胞

Product Overview

Porcine Coronary Artery Smooth Muscle cells are isolated, expanded, and cryopreserved in a manner similar to Lonza’s Human Coronary Artery Smooth Muscle Cells.

Catalog no. 00227730 (Lot no. 18TL113331)
Species: Porcine
Culture Media: Lonza SmGM^TM-2 Smooth Muscle Cell Growth Medium -2 BulletKit^TM (Lonza Cat. No. CC-3182)
Culture Conditions: Culture following Lonza’s Standard Protocol for culturing Human Smooth Muscle Cells
Quality Control Testing: Cell Count (476,900 cells/vial), Viability (95%), Mycoplasma (not detected), Sterility (negative)

该产品被归类为限量版细胞类型。 Lonza CellBio Services 有时会从定制分离物中获得多余或额外的细胞，这些细胞可作为单独的冷冻保存小瓶提供。这些限量版细胞在有限的库存中可用，并且在库存耗尽后可能不再可用。

Content & Storage

Content

1 x Cryopreserved Ampule with 476,900 cells/vial

Store at -180 C

lonza BEBP12-934Q,原代细胞和干细胞,TheraPEAK™ MSCBM™ 基础培养基 – 1 L

Catalog #: BEBP12-934Q

TheraPEAK^TM MSCGM^TM Mesenchymal Stem Cell Basal Medium is a GMP-produced, serum-free cell medium without gentamicin and phenol red, 1 L.

Product Overview

干细胞具有作为未分化细胞类型增殖的独特能力，然后在适当刺激时分化成多个细胞谱系。 Lonza 的 TheraPEAKTM MSCGMTM 间充质干细胞培养基是一种无血清培养基，旨在在未分化状态下增殖人骨髓来源的间充质细胞。该培养基经过专门设计和测试，以确保该培养基既不促进细胞的自发分化，也不抑制细胞向成骨、软骨或脂肪谱系的分化（当用适当的分化培养基适当刺激时）。无血清培养基，例如 TheraPEAKTM MSCGMTM 间充质干细胞培养基，旨在支持细胞生长而无需补充血清。 Lonza 的 TheraPEAKTM MSCGMTM 间充质干细胞培养基是那些寻求最大限度控制研究和制造变量的人的主要兴趣所在。 TheraPEAKTM MSCGMTM 间充质干细胞培养基不含酚红或抗生素。

使用 TheraPEAKTM MSCGMTM 间充质干细胞培养基增殖的部分细胞列表*：

人骨髓来源的干细胞
人牙周膜来源干细胞
人体脂肪干细胞
TheraPEAKTM MSCGMTM 间充质干细胞培养基以 BulletKitTM 培养基（目录号 BEBP18-936）的形式提供，其中包括基础培养基和人骨髓来源间充质干细胞增殖所需的补充剂。基础培养基（MSCBM 间充质基础培养基；目录号 BEBP12-934Q）和必要的补充剂（MSCGMTM SingleQuotsTM 试剂盒；目录号 BEBP13-935Z5）也可以单独购买。

*从客户数据生成的单元格列表。龙沙仅常规将此培养基与龙沙的人骨髓间充质干细胞一起使用，因此只能保证此培养基与这些细胞的成功使用。所有 TheraPEAKTM 培养基均为 GMP 产品，旨在支持进一步的生产，并按照 GMP 原材料标准生产。这些媒体产品符合医疗设备的 cGMP 制造合规预期，包括 21 CFR Part 820 和 ISO 13485。TheraPEAKTM 媒体产品在 FDA 注册的生产基地生产，具有 ISO 13485 认证的质量管理体系。所有仅用于研究的配方均根据 ISO:9001 生产。

lonza BEBP18-936,原代细胞和干细胞,TheraPEAKTM MSCGMTM 间充质干细胞生长培养基 BulletKitTM

Catalog #: BEBP18-936

TheraPEAK^TM MSCGM^TM Mesenchymal Stem Cell Growth Medium BulletKit^TM including the basal medium and supplements, which is GMP-produced and serum-free, 1 L.

Product Overview

干细胞具有作为未分化细胞类型增殖的独特能力，然后在适当刺激时分化成多个细胞谱系。 Lonza 的 TheraPEAKTM MSCGMTM 间充质干细胞生长培养基是一种无血清培养基，旨在增殖处于未分化状态的人骨髓来源的间充质细胞。该培养基经过专门设计和测试，以确保该培养基既不促进细胞的自发分化，也不抑制细胞向成骨、软骨或脂肪谱系的分化（当用适当的分化培养基适当刺激时）。无血清培养基，例如 TheraPEAKTM MSCGMTM 间充质干细胞生长培养基，旨在支持细胞生长而无需补充血清。 Lonza 的 TheraPEAKTM MSCGMTM 间充质干细胞生长培养基是那些寻求最大限度控制研究和制造变量的人的主要兴趣所在。 TheraPEAKTM MSCGMTM 间充质干细胞培养基不含酚红或抗生素。

使用 TheraPEAKTM MSCGMTM 间充质干细胞生长培养基增殖的部分细胞列表*：

人骨髓来源的干细胞
人牙周膜来源干细胞
人体脂肪干细胞
TheraPEAKTM MSCGMTM 间充质干细胞生长培养基以 BulletKitTM 培养基（目录号 BEBP18-936）的形式提供，其中包括基础培养基和人骨髓来源间充质干细胞增殖所需的补充剂。

*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。所有 TheraPEAKTM 培养基都是 GMP 产品，旨在支持进一步的生产，并按照 GMP 原材料标准生产。这些媒体产品符合医疗设备的 cGMP 制造合规预期，包括 21 CFR Part 820 和 ISO 13485。TheraPEAKTM 媒体产品在 FDA 注册的生产基地生产，具有 ISO 13485 认证的质量管理体系。所有仅用于研究的配方均根据 ISO:9001 生产。

Benefits

Serum-free and regulatory friendly
No need for attachment matrix to plate cells
Optimized for multiple expansion of all types of hMSCs
Supports multi-lineage differentiation
Provided in BulletKit^TM Format, includes basal medium and supplement
Custom packaging available to meet scale up needs
Can be used in small or large scale therapeutic applications

lonza,原代细胞和干细胞,lonza 00225974,hVIC – 人瓣膜间质细胞

Catalog #: 00225974

Custom, made-to-order batch of Human Valvular Interstitial Cells containing 20 vials containing ≥500,000 cells per vial.

Request Information or Quote or contact Lonza CellBio Services to discuss standard or custom production requests or to obtain more information.

Overview

Product Overview

The aortic valve is a valve in the human heart between the left ventricle and the aorta. The valve undergoes exceptional forces due to blood flow during each cardiac cycle. The two primary cell types in the aortic valve are the aortic valve endothelial cells (HAoVEC) which provide the barrier function as the valve opens and closes and the valvular interstitial Cells (hVIC) which provide the structural integrity of the valve.

Lonza’s Human Valvular Interstitial Cells (hVIC) are provided as a made to order product by Lonza’s CellBio Services custom cell isolation team.

Cryopreserved Human Valvular Interstitial Cells (hVIC) are tested for expression levels of Factor VIII and Alpha Smooth Muscle Actin, viability, plating efficiency, and doubling time. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

This product can be provided on its own or as a matched set with Human Aortic Valve Endothelial Cells (HAoVEC; part number 00225975). This product is also customizable to allow for larger batch sizes, additional testing specifications, or specific donor requirements.

主动脉瓣是人心脏中左心室和主动脉之间的瓣膜。由于每个心动周期中的血流，瓣膜会受到异常的作用力。主动脉瓣中的两种主要细胞类型是在瓣膜打开和关闭时提供屏障功能的主动脉瓣内皮细胞 (HAoVEC) 和提供瓣膜结构完整性的瓣膜间质细胞 (hVIC)。

Lonza 的人瓣膜间质细胞 (hVIC) 作为定制产品由 Lonza 的 CellBio Services 定制细胞分离团队提供。

检测冷冻保存的人瓣膜间质细胞 (hVIC) 的因子 VIII 和 Alpha 平滑肌肌动蛋白的表达水平、活力、铺板效率和倍增时间。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

该产品可以单独提供，也可以与人主动脉瓣内皮细胞（HAoVEC；部件号 00225975）配套提供。该产品还可以定制，以允许更大的批量、额外的测试规范或特定的捐助者要求。

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate of Analysis is provided for each cell lot purchased

Content & Storage

Content

1 batch containing 20 vials containing ≥500,000 cells per vial

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

hVIC - Human Valvular Interstitial Cells

AoAF – Human Aortic Adventitial Fibroblasts CC-7014

NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904

NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903

hAoVEC – Human Aortic Valve Endothelial Cells 00225975

MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001

lonza,原代细胞和干细胞,lonza 260260.01,冷冻人白细胞(5.0 Billion Cells)

Catalog #: 260260.01

Cryopreserved human leukapheresis product collected from normal peripheral blood, containing ≥5.0 billion cells.

从正常外周血中采集的冷冻保存的人白细胞分离产品，含有≥50亿个细胞。

Overview

Product Overview

A leukopak is a highly-enriched leukapheresis-derived product obtained from an IRB-approved collection facility. Leukopaks contain higher concentrations of mononuclear cells, B cells, T cells, stem/progenitor cells, dendritic cells, and other cell types as compared to standard venipuncture collection methods or buffy coat products.

The total volume and concentration of the supplied cells will vary. Based on this count, the product is filled to deliver not less than 5.0 billion cells upon thaw.

Leukopaks typically contain up to 50% T cells, 20% monocytes, 10% B cells, and 10% NK cells, 3% granulocytes, and 3 % hematocrit. Actual percentages will vary from donor to donor.

Cyropreserved leukopaks are frozen quickly after collection using a controlled rate method to maximize viability.

Additional shipping charges apply for cryopreserved leukopaks.

leukopak 是从 IRB 批准的收集设施中获得的高度富集的白细胞分离衍生产品。与标准静脉穿刺采集方法或血沉棕黄层产品相比，Leukopaks 含有更高浓度的单核细胞、B 细胞、T 细胞、干细胞/祖细胞、树突细胞和其他细胞类型。

提供的细胞的总体积和浓度会有所不同。基于此计数，该产品在解冻后被填充以提供不少于 50 亿个细胞。

Leukopaks 通常含有高达 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞和 10% 的 NK 细胞、3% 的粒细胞和 3% 的血细胞比容。实际百分比会因捐赠者而异。

冷冻保存的白细胞在收集后使用受控速率方法快速冷冻，以最大限度地提高生存能力。

冷冻保存的白细胞需支付额外的运费。

Benefits

Post thaw viability >95%
guaranteed minimum quantity of MNCs from a single donor
higher concentration of leukocytes compared to buffy coats
IRB-approved donor management and collection process
解冻后存活率 >95%
保证来自单一捐助者的跨国公司的最低数量
与血沉棕黄层相比，白细胞浓度更高
IRB 批准的捐赠者管理和收集流程

Content & Storage

Content

1 collection bag containing ≥5.0 billion cryopreserved cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Related Products

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

Fresh Human Leukopak (5 Billion Cells) 3P-105B

lonza,原代细胞和干细胞,Human Liver-Derived Endothelial Cells P1

Catalog #: HLECP1

Low passage endothelial cells dervied from human liver tissue

Overview

Product Overview

Endothelial cells are the primary barrier cells that line blood vessels and sinusoids of the liver. Human Liver-derived Endothelial Cells (HLECs) are isolated and enriched from liver tissue, then cryopreserved for use in a wide range of research models. A unique subtype of LECs, liver sinusoidal endothelial cells, are defined as a subset of cells found at the interface between the liver sinusoids and hepatocytes.

HLECs derived from whole livers lead to a mixed population of vascular endothelial cells and sinusoidal endothelial cells. HLECs characterized for markers of endothelial cells, including CD31 and vWF maintain appropriate endothelial morphology over limited serial passaging. Measurement of uptake of acetylated LDL to indicate percentage of the sinusoidal endothelial cell population. The recommended medium for HLECs is EGMTM 2 which is optimized for endothelial cell health and growth.

Benefits

Low passage
Verified for large molecule uptake

Content & Storage

Content

Cryopreserved Cells, >1 Million viable cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

FAQ & Citations

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