MCE抑制剂∣Abcam CST Santa抗体∣蛋白、酶、磁珠
货号：45152105050250描述：Sera-Mag SpeedBead 羧基修饰快速磁珠 (亲水性), 15 mL
Sera-Mag SpeedBeads和SeSera-Mag Carboxylate-Modified Magnetic Particles磁珠不仅具有快速的磁响应时间，而且通过大的表面积带来更高的载量，同时具有高灵敏度，稳定性，物理完整性和快速反应动力学特性。 生物分子如蛋白质和核酸与表面羧基的共价偶联很容易用我们的标准偶联技术完成。 典型应用包括样品制备、蛋白质组学、核酸分离和免疫分析应用。
颗粒的核心由苯乙烯和酸单体的自由基乳液聚合制成。然后将一个（Sera-Mag）或两个（Sera-Mag SpeedBeads）磁铁包被到该芯上，其表面经过修饰可以降低蛋白质的非特异性结合。
Reagents > Intercept® (PBS) Blocking Buffer
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system.
For larger pack sizes, please contact your local sales office.
Intercept (PBS) 封闭缓冲液经过配制，可为可复制的定量蛋白质印迹和其他免疫测定提供高效封闭和低背景变异性。 当使用磷酸盐缓冲盐水 (PBS) 系统时，Intercept (PBS) 封闭缓冲液是近红外荧光应用的首选封闭缓冲液。
不含哺乳动物蛋白，与哺乳动物抗体的交叉反应性最低。
生物素-链霉亲和素检测的绝佳选择。
适用于广泛的应用，包括蛋白质印迹、In-Cell Western™ 检测和蛋白质阵列。
如需更大的包装尺寸，请联系您当地的销售办事处。
Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.
Intercept (PBS) 封闭缓冲液是 PBS 中的即用型封闭剂配方。 它不含叠氮化钠，因此适用于近红外或化学发光检测。 它在 4 °C 下储存，也可用于包含 Immobilon®-FL PVDF 和硝酸纤维素膜的试剂盒中。
Shake well before each use.
Intercept (PBS) Blocking Buffer can be used for many immunoassays and applications, including:
货号：65152105050250描述：Sera-Mag SpeedBead 羧基修饰快速磁珠(疏水性), 15 mL
Sera-Mag SpeedBeads和SeSera-Mag Carboxylate-Modified Magnetic Particles磁珠不仅具有快速的磁响应时间，而且通过大的表面积带来更高的载量，同时具有高灵敏度，稳定性，物理完整性和快速反应动力学特性。 生物分子如蛋白质和核酸与表面羧基的共价偶联很容易用我们的标准偶联技术完成。 典型应用包括样品制备、蛋白质组学、核酸分离和免疫分析应用。
颗粒的核心由苯乙烯和酸单体的自由基乳液聚合制成。然后将一个（Sera-Mag）或两个（Sera-Mag SpeedBeads）磁铁包被到该芯上，其表面经过修饰可以降低蛋白质的非特异性结合。
Reagents > Intercept® (TBS) Blocking Buffer
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS).
For larger pack sizes, please contact your local sales office.
Intercept (TBS) 封闭缓冲液经过配制，可为可复制的定量蛋白质印迹和其他免疫测定提供高效封闭和低背景变异性。 Intercept (TBS) 封闭缓冲液是一种在 Tris 缓冲盐水 (TBS) 中的即用型封闭剂配方。
不含哺乳动物蛋白，与哺乳动物抗体的交叉反应性最低。
可用于最广泛的蛋白质印迹检测目标。
非常适合针对基于 Tris 的缓冲系统进行优化的一抗。
可用于检测磷酸化目标。
具有卓越的碱性磷酸酶检测性能。
生物素-链霉亲和素检测的绝佳选择。
如需更大的包装尺寸，请联系您当地的销售办事处。
Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in TBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.
Intercept (TBS) 封闭缓冲液是 TBS 中的即用型封闭剂配方。 它不含叠氮化钠，因此适用于近红外或化学发光检测。 它在 4 °C 下储存，也可用于包含 Immobilon®-FL PVDF 和硝酸纤维素膜的试剂盒中。
Shake well before each use.
Intercept (TBS) Blocking Buffer can be used for many immunoassays and applications, including:
Reagents > Intercept® (PBS) Protein-Free Blocking Buffer
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited.
For larger pack sizes, please contact your local sales office.
Intercept (PBS) 无蛋白封闭缓冲液经过配制，可为可复制的定量蛋白质印迹和其他免疫测定提供高效封闭和低背景变异性。 该缓冲液不含蛋白质，用于禁止使用动物产品的分析。
生物素-链霉亲和素检测的绝佳选择。
适用于广泛的应用，包括蛋白质印迹、In-Cell Western™ 检测和蛋白质阵列。
如需更大的包装尺寸，请联系您当地的销售办事处。
Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Intercept (PBS) 无蛋白封闭缓冲液是一种在磷酸盐缓冲盐水中的即用型封闭剂配方，可在 4 °C 下储存。 它不含叠氮化钠，因此适用于近红外或化学发光检测。
Shake well before each use.
Intercept (PBS) Protein-Free Blocking Buffer can be used for many immunoassays and applications, including:
Intercept® T20 (TBS) Antibody Diluent
Intercept® T20 (PBS) Antibody Diluent
Intercept® T20 (TBS) Protein-Free Antibody Diluent
Intercept® T20 (PBS) Protein-Free Antibody Diluent
Intercept® (TBS) Blocking Buffer and Diluent Kit
Intercept® (PBS) Blocking Buffer and Diluent Kit
Intercept T20 Antibody Diluents for use with Odyssey® Imaging Systems, are optimized for use with IRDye® Infrared Dye Reagents and other near-infrared fluorophores. All Intercept T20 Antibody Diluents provide excellent performance for quantitative and chemiluminescent Western blots, as well as other immunoassays.
用于 Odyssey® 成像系统的 Intercept T20 抗体稀释剂经过优化，可用于 IRDye® 红外染料试剂和其他近红外荧光团。 所有 Intercept T20 抗体稀释剂都为定量和化学发光蛋白质印迹以及其他免疫测定提供了出色的性能。
Antibody diluents are used for the dilution of primary antibodies and secondary antibodies to the working concentration required for your experiments. Antibody diluents improve the specificity of primary and secondary antibodies, reducing off-target effects. This reduces background due to non-specific binding.
Intercept T20 Antibody Diluents are preformulated in-house with Tween® 20, a non-ionic detergent. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation.
抗体稀释剂用于将一抗和二抗稀释至实验所需的工作浓度。 抗体稀释剂可提高一抗和二抗的特异性，减少脱靶效应。 由于非特异性结合，这减少了背景。
Intercept T20 抗体稀释剂在内部使用非离子去污剂 Tween® 20 预先配制。 无需自己混合稀释剂，这样可以节省您的时间并减少潜在的变化。
Intercept T20 Antibody Diluents can be used in many applications, including:
货号：44152105050250描述：Sera-Mag 羧基修饰磁珠 (亲水性), 15 mL
Sera-Mag SpeedBeads和SeSera-Mag Carboxylate-Modified Magnetic Particles磁珠不仅具有快速的磁响应时间，而且通过大的表面积带来更高的载量，同时具有高灵敏度，稳定性，物理完整性和快速反应动力学特性。 生物分子如蛋白质和核酸与表面羧基的共价偶联很容易用我们的标准偶联技术完成。 典型应用包括样品制备、蛋白质组学、核酸分离和免疫分析应用。
颗粒的核心由苯乙烯和酸单体的自由基乳液聚合制成。然后将一个（Sera-Mag）或两个（Sera-Mag SpeedBeads）磁铁包被到该芯上，其表面经过修饰可以降低蛋白质的非特异性结合。
Reagents > Intercept® (TBS) Protein-Free Blocking Buffer
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS).
Intercept (TBS) 无蛋白封闭缓冲液经过配制，可为可复制的定量蛋白质印迹和其他禁止使用动物产品的免疫分析提供高效封闭和低背景变异性。 Intercept (TBS) 无蛋白封闭缓冲液是一种在 Tris 缓冲盐水 (TBS) 中的即用型封闭剂配方。
For larger pack sizes, please contact your local sales office.
Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Intercept (TBS) Protein-Free Blocking Buffer 是 TBS 中的即用型封闭剂配方，可在 4 °C 下储存。 它不含叠氮化钠，因此适用于近红外或化学发光检测。
Shake well before each use.
Intercept (TBS) Protein-Free Blocking Buffer can be used for many immunoassays and applications, including:
货号：24152105050250描述：Sera-Mag 羧基修饰磁珠 (亲水性), 15 mL
Sera-Mag SpeedBeads和Sera-Mag Carboxylate-Modified Magnetic Particles磁珠不仅具有快速的磁响应时间，而且通过大的表面积带了跟高的载量，同时具有高灵敏度，稳定性，物理完整性和快速反应动力学特性。 生物分子如蛋白质和核酸与表面羧基的共价偶联很容易用我们的标准偶联技术完成。 典型应用包括样品制备、蛋白质组学、核酸分离和免疫分析应用。
颗粒的核心由苯乙烯和酸单体的自由基乳液聚合制成。然后将一个（Sera-Mag）或两个（Sera-Mag SpeedBeads）磁铁矿层涂覆到该芯上，而表面被改性，以最小化蛋白质的非特异性结合。
Reagents > Intercept® T20 (PBS) Protein-Free Antibody Diluent
Intercept T20 (PBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Protein-Free Blocking Buffer preformulated with Tween® 20 for use in a phosphate-buffered saline (PBS) system. This diluent can be used where animal-based products are prohibited.
Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation.
Intercept T20 (PBS) 无蛋白抗体稀释剂可提高一抗和二抗的特异性，减少脱靶效应。 稀释剂包含用 Tween® 20 预先配制的 Intercept (PBS) 无蛋白封闭缓冲液，用于磷酸盐缓冲盐水 (PBS) 系统。 这种稀释剂可用于禁止动物性产品的地方。
由于无需自己混合稀释剂，因此可以节省您的时间并减少潜在的变化。
Intercept T20 (PBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Intercept T20 (PBS) Protein-Free Antibody Diluent 含有 0.05% 浓度的 Tween 20。它不含叠氮化钠，并在 4 °C 下储存。
Shake well before each use.
Intercept T20 (PBS) Protein-Free Antibody Diluent can be used for many immunoassays and applications, including:
货号：28985799描述：Streptavidin Mag Sepharose 5*1mL
Streptavidin Mag Sepharose磁珠可以简单高效的通过免疫沉淀富集靶蛋白，同时也可用于生物素化生物分子的纯化。Streptavidin Mag Sepharose，利用生物素和链霉亲和素配体（固定在磁珠上）之间的强相互作用。磁珠简化了小规模样品纯化的处理流程。磁珠有两种包装规格可供选择： 2 ?— 1 ml 10% 悬液 and 5 ?— 1 ml 10% 悬液.一mL10% 介质浆料和100 ??l已沉淀介质相同，按照建议的免疫沉淀反应操作方案，足够用于20次纯化。
Reagents > Intercept® T20 (TBS) Antibody Diluent
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween® 20.
Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation.
Intercept T20 (TBS) Antibody Diluent 提高一抗和二抗的特异性，减少脱靶效应。 稀释剂含有用 Tween® 20 预先配制的 Tris 缓冲盐水 (TBS) 中的拦截 (TBS) 封闭缓冲液。
由于无需自己混合稀释剂，因此可以节省您的时间并减少潜在的变化。
Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Shake well before each use.
Intercept T20 (TBS) Antibody Diluent can be used for many immunoassays and applications, including:
货号：28985738描述：Streptavidin Mag Sepharose 2*1mL
Streptavidin Mag Sepharose磁珠可以简单高效的通过免疫沉淀富集靶蛋白，同时也可用于生物素化生物分子的纯化。Streptavidin Mag Sepharose，利用生物素和链霉亲和素配体（固定在磁珠上）之间的强相互作用。磁珠简化了小规模样品纯化的处理流程。磁珠有两种包装规格可供选择： 2 ?— 1 ml 10% 悬液 and 5 ?— 1 ml 10% 悬液.一mL10% 介质浆料和100 ??l已沉淀介质相同，按照建议的免疫沉淀反应操作方案，足够用于20次纯化。
Intercept® (TBS) Blocking Buffer,Intercept® (TBS) 封闭缓冲液
Intercept® (PBS) Blocking Buffer,Intercept® (PBS) 封闭缓冲液
Intercept® (TBS) Protein-Free Blocking Buffer,Intercept® (TBS) 无蛋白封闭缓冲液
Intercept® (PBS) Protein-Free Blocking Buffer,Intercept® (PBS) 无蛋白封闭缓冲液
Intercept® (TBS) Blocking Buffer and Diluent Kit,Intercept® (TBS) 封闭缓冲液和稀释剂试剂盒
Intercept® (PBS) Blocking Buffer and Diluent Kit,Intercept® (PBS) 封闭缓冲液和稀释剂试剂盒
Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye 800CW Goat anti-Mouse IgG and IRDye 680LT Goat anti-Rabbit IgG, and scanned together on an Odyssey CLx Imaging System.
图 1：Western 印迹协议，展示了 Intercept Blocking Buffer 与 Odyssey Blocking Buffer 的比较。 用连续稀释的小鼠心脏组织提取物检测 Stat3 Mouse mAb 和 p38 MAPK Rabbit Ab，用 IRDye 800CW 山羊抗小鼠 IgG 和 IRDye 680LT 山羊抗兔 IgG 检测，并在 Odyssey CLx 成像系统上一起扫描。
Reagents > Intercept® T20 (TBS) Protein-Free Antibody Diluent
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween® 20. This diluent can be used where animal-based products are prohibited.
Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation.
Intercept T20 (TBS) 无蛋白抗体稀释剂可提高一抗和二抗的特异性，减少脱靶效应。 稀释剂含有在 Tris 缓冲盐水 (TBS) 中预先配制有 Tween® 20 的 Intercept (TBS) 无蛋白封闭缓冲液。这种稀释剂可用于禁止使用动物产品的地方。
由于无需自己混合稀释剂，因此可以节省您的时间并减少潜在的变化。
Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Intercept T20 (TBS) Protein-Free Antibody Diluent 含有浓度为 0.05% 的 Tween 20。它不含叠氮化钠，并在 4 °C 下储存。
Shake well before each use.
Intercept T20 (TBS) Protein-Free Antibody Diluent can be used for many immunoassays and applications, including:
货号：28951377描述：TiO2 Mag Sepharose 4*500ul
TiOa?? Mag Sepharose磁珠是以二氧化钛（TiOa??）层析为基础，旨在简化磷酸肽的捕获和富集。TiOa?? 对磷酸肽有很高的亲和性，是一种从复合样品中富集磷酸肽的有效方法。
TiOa?? Mag Sepharose提高了磷酸肽制备过程中的易用性。磁珠具有小规模实验的优良特性。磁珠的高密度允许磁性装置件快速捕获，而磁珠的可见性保证了所有结合的目标肽的可靠收集。
MagRack 6是一个分离工具，可与6个离心管联用，实现最多可并行处理六个样品。
CellVue® Burgundy Fluorescent Cell Labeling Kit,CellVue® 酒红色荧光细胞标记试剂盒
CellVue® NIR815 Fluorescent Cell Labeling Kit,CellVue® NIR815 荧光细胞标记试剂盒
Diluent C for Use with CellVue® Fluorescent Cell Labeling Kits,用于 CellVue® 荧光细胞标记试剂盒的稀释剂 C
CellVue fluorescent imaging kits use proprietary labeling technology to stably incorporate fluorescent dyes containing long aliphatic hydrocarbon tails into lipid membranes1. They are useful for researchers working in all aspects of science and technology where fluorescently-labeled cells and/or tissues are required. CellVue dyes also provide researchers with valuable tools for many in vivo and in vitro cell studies using fluorescent membrane labels.
The CellVue dyes are provided in an easy-to-use kit format containing a solution of the dye in ethanol and a cell labeling diluent. The labeling diluent, Diluent C, provided with the kit is an iso-osmotic aqueous solution that contains no physiologic salts or buffers, detergents, or organic solvents, and is designed to maintain cell viability while maximizing dye solubility and staining efficiency2, 3. The simple membrane labeling protocol provided with the kit is applicable to almost any type of cell or organism.
CellVue 荧光成像试剂盒使用专有的标记技术将含有长脂肪烃尾的荧光染料稳定地结合到脂质膜中1。 它们对于在需要荧光标记的细胞和/或组织的科学和技术的各个方面工作的研究人员非常有用。 CellVue 染料还为研究人员提供了许多使用荧光膜标记进行体内和体外细胞研究的宝贵工具。
CellVue 染料以易于使用的试剂盒形式提供，其中包含染料乙醇溶液和细胞标记稀释剂。 试剂盒随附的标记稀释剂 Diluent C 是一种等渗水溶液，不含生理盐或缓冲液、去污剂或有机溶剂，旨在保持细胞活力，同时最大限度地提高染料溶解度和染色效率 2, 3。 试剂盒提供的简单膜标记方案适用于几乎任何类型的细胞或生物体。
CellVue dyes consist of long aliphatic hydrocarbon tails linked to a polar fluorescent chromophore.
Reagents > 10X Orange Loading Dye
10X Orange Loading Dye is used as a loading buffer for DNA gel electrophoresis. This loading dye is not visualized when imaged on Odyssey® Imagers. The primary application for 10X Orange Loading Dye is for electrophoretic mobility shift assays (EMSA). When run on a 5% native acrylamide 1X TBE gel, the orange dye migrates with the 20-28 bp DNA fragment.
10X Orange Loading Dye 用作 DNA 凝胶电泳的上样缓冲液。 当在 Odyssey® Imagers 上成像时，这种加载染料不可见。 10X Orange Loading Dye 的主要应用是电泳迁移率变动分析 (EMSA)。 在 5% 天然丙烯酰胺 1X TBE 凝胶上运行时，橙色染料与 20-28 bp DNA 片段一起迁移。
货号：28944010描述：TiO2 Mag Sepharose 1*500ul
TiOa?? Mag Sepharose磁珠是以二氧化钛（TiOa??）层析为基础，旨在简化磷酸肽的捕获和富集。TiOa?? 对磷酸肽有很高的亲和性，是一种从复合样品中富集磷酸肽的有效方法。
TiOa?? Mag Sepharose提高了磷酸肽制备过程中的易用性。磁珠具有小规模实验的优良特性。磁珠的高密度允许磁性装置件快速捕获，而磁珠的可见性保证了所有结合的目标肽的可靠收集。
MagRack 6是一个分离工具，可与6个离心管联用，实现最多可并行处理六个样品。
Accessories > 4-Inch Soft Roller
The roller is used to eliminate air bubbles from membranes on the glass surface of the Odyssey® M or Odyssey DLx Imager.
It can also be used when placing membranes or gels on the Odyssey XF imaging tray (P/N 926-40020).
滚筒用于消除 Odyssey® M 或 Odyssey DLx Imager 玻璃表面膜上的气泡。
它也可用于在 Odyssey XF 成像托盘（P/N 926-40020）上放置膜或凝胶。
IRDye® DBCO Group,IRDye® DBCO 集团
IRDye® Alkyne Group,IRDye® 炔烃基团
IRDye® Azide Group,IRDye® 叠氮化物组
Biomolecule labeling continues to be a cornerstone feature of many in vitro and in vivo biological experiments. Click Chemistry has become convenient and reliable method for labeling a wide variety of molecules for applications ranging from biomarker isolation to assay development.
生物分子标记仍然是许多体外和体内生物实验的基石特征。 Click Chemistry 已成为一种方便且可靠的方法，可用于标记从生物标志物分离到分析开发等各种应用的各种分子。
Click Chemistry reagents from LI-COR offer several benefits:
Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally occurring functional groups such as amines.
Click Chemistry reactions can be categorized into two separate groups, copper-catalyzed or copper-free. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes. Although they initiate and accelerate Click Reactions, copper catalysts are cytotoxic and inappropriate for use in living systems.
To address this limitation, copper-free methods have been developed to allow Click Chemistry with live cells and whole organisms.
Click Chemistry 使用成对的试剂，它们相互之间发生专门的反应，并且对天然存在的官能团（如胺）有效地呈惰性。
Click Chemistry 反应可分为两个独立的组，铜催化的或无铜的。 铜催化点击化学用于引发叠氮化物和炔烃之间的反应。 虽然它们会引发和加速点击反应，但铜催化剂具有细胞毒性，不适合在生命系统中使用。
为了解决这一限制，已开发出无铜方法以允许使用活细胞和整个生物体进行 Click Chemistry。
货号：28944009描述：NHS Mag Sepharose 1*500ul
NHS Mag Sepharose是为pull-down技术设计的磁珠，能够快速捕获和富集选定的蛋白质。将感兴趣的抗体或其它蛋白质通过伯胺共价偶联到基架上，用于捕获目标靶蛋白。
Reagents > 4X Protein Sample Loading Buffer for Western Blots
4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange loading buffer is recommended for use with Odyssey® Imaging Systems as it does not fluoresce in the 700 nm channel the way blue loading buffers do. The buffer is optimized for use with SDS-PAGE and Tris-Glycine-SDS running buffer.
4X 蛋白质上样缓冲液经过优化，可用作蛋白质凝胶电泳的上样缓冲液。 建议将此橙色上样缓冲液与 Odyssey® Imaging Systems 一起使用，因为它不会像蓝色上样缓冲液那样在 700 nm 通道中发出荧光。 该缓冲液经过优化，可与 SDS-PAGE 和 Tris-Glycine-SDS 运行缓冲液一起使用。
Odyssey® EMSA Kit,Odyssey® EMSA 套件
10X Orange Loading Dye,10X 橙色负载染料
IRDye® 700 Dye-Labeled Oligonucleotides,IRDye® 700 染料标记寡核苷酸
Mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.
A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey® Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol.
通过用 IRDye® 红外染料末端标记的寡核苷酸替换现有的 DNA 寡核苷酸，迁移率变化分析方案可以轻松转换为红外荧光分析。 结合和电泳条件与任何其他 EMSA 检测方法相同。
用 IRDye 700 红外染料末端标记的 DNA 寡核苷酸是蛋白质结合的良好底物。 使用 Odyssey® 红外成像系统，IRDye 红外染料标记的 DNA 检测在 9.1 fmol 至 0.18 fmol 的 50 倍稀释范围内是线性的。
Infrared fluorescent assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. If you are not satisfied that the electrophoresis has progressed far enough, you can place the gel back into the electrophoresis unit and run longer.
Other IRDye 700 oligonucleotides as well as IRDye 800 oligonucleotides are available through Integrated DNA Technologies, TriLink BioTechnologies, or Metabion International AG.
其他 IRDye 700 寡核苷酸和 IRDye 800 寡核苷酸可通过 Integrated DNA Technologies、TriLink BioTechnologies 或 Metabion International AG 获得。
| Infrared | Radioisotope |
|---|---|
| Easy access and disposal | Short half-life of the label |
| Dye is stable for a long time | Regulatory procedures Disposal limitations |
| Non-hazardous | Hazardous |
| Gel (glass plates) can be easily imaged on the Odyssey | Lengthy incubations with autoradiographic film |
| Detection of the probe is rapid | Time-consuming and inconvenient |
| Gel can be replaced back and run longer | Not possible |
货号：28951380描述：NHS Mag Sepharose 4*500ul
NHS Mag Sepharose是为pull-down技术设计的磁珠，能够快速捕获和富集选定的蛋白质。将感兴趣的抗体或其它蛋白质通过伯胺共价偶联到基架上，用于捕获目标靶蛋白。
Cytiva磁珠,NHS Mag Sepharose 4x500ul,Cytiva磁珠NHS Mag琼脂糖凝胶
Reagents > 4X Protein Sample Loading Buffer and PVDF Membrane Kit
4X Protein Sample Loading Buffer (P/N 928-40004) is optimized for use as a loading buffer for protein gel electrophoresis. This orange-colored loading buffer has no dyes that may cause background as compare to other loading buffers with a blue dye component that can increase background.
The Immobilon®-FL PVDF membrane included in these kits has been tested to ensure a low-background for near-infrared fluorescent Western blot success.
4X 蛋白质上样缓冲液（P/N 928-40004）经过优化，可用作蛋白质凝胶电泳的上样缓冲液。 与其他含有会增加背景的蓝色染料成分的上样缓冲液相比，这种橙色上样缓冲液不含可能导致背景的染料。
这些试剂盒中包含的 Immobilon®-FL PVDF 膜已经过测试，以确保近红外荧光蛋白质印迹成功的低背景。
CellTag™ 700 Stain,CellTag™ 700 染色剂
GAM CellTag™ 700 Stain ICW Kit I,GAM CellTag™ 700 染色 ICW 试剂盒 I
GAR CellTag™ 700 Stain ICW Kit II,GAR CellTag™ 700 染色 ICW 试剂盒 II
CellTag™ 520 Stain,CellTag™ 520 染色剂
GAM/GAR CellTag™ 520 Stain ICW Kit III,GAM/GAR CellTag™ 520 染色 ICW 试剂盒 III
GAR/GAM CellTag™ 520 Stain ICW Kit IV,GAR/GAM CellTag™ 520 染色 ICW 试剂盒 IV
96-Well Plate for In-Cell Western™ Assays,用于 In-Cell Western™ 检测的 96 孔板
The In-Cell Western Assay is an immunocytochemical assay that uses fluorescence to detect and quantify proteins in cells that have been cultured in microplates. Detecting proteins in their cellular context increases quantification precision.
This process allows for higher throughput compared to Western blotting and eliminates typical Western blotting steps such as cell lysate preparation, electrophoresis, and membrane transfer. Cost per well is reduced to a fraction of the cost of typical screening methods with CellTag™ Stain In-Cell Western Kits.
CellTag Stains are a fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag Stains are applied to the cells during incubation with IRDye® Secondary Antibodies and enable accurate measurement of target protein levels with much higher throughput than Western blotting.
In-Cell Western 检测是一种免疫细胞化学检测，它使用荧光检测和量化微孔板中培养的细胞中的蛋白质。在细胞环境中检测蛋白质可提高定量精度。
与蛋白质印迹法相比，该过程允许更高的通量，并消除了典型的蛋白质印迹法步骤，例如细胞裂解物制备、电泳和膜转移。使用 CellTag™ Stain In-Cell Western Kits 将每孔成本降低到典型筛选方法成本的一小部分。
CellTag 染色剂是一种荧光非特异性细胞染色剂，可为 In-Cell Western 应用提供准确的细胞数标准化。该染料在透化细胞的细胞核和细胞质中积累，并在各种细胞类型和细胞数量中提供线性荧光信号。在与 IRDye® 二抗孵育期间将 CellTag Stains 应用于细胞，并能够以比蛋白质印迹高得多的通量准确测量靶蛋白水平。
货号：28967070描述：Protein G Mag Sepharose Xtra 5*1mL
Protein G Mag Sepharose Xtra磁珠结合成熟的亲和层析方法 和磁珠平台，提高样品制备过程中的简易性和易用性。Mag Sepharose磁珠特征包括密度高、黑色和可可视性，操作非常简单。磁珠更低的拖尾效应和聚集沉淀意味着缓冲液不需要使用表面活性剂。
结合MagRack 6，用于微量离心管的分离工具，可以并行处理六份样品。这样，你可以在高通量条件下轻松并行筛选大量样品。Protein G Mag Sepharose Xtra提供两种版本，2 ?— 1 ml包装，可使用20次提纯，5 ?— 1 ml包装，可使用50次提纯。产品将磁包含在琼脂糖中制备而成，配基是天然蛋白质G。蛋白质G有较高亲和力，适合于各种生物IgG抗体的Fc区域。
Protein G Mag Sepharose是Mag Sepharose 平台的另一种产品，可以用于免疫沉淀反应技术，用于从细胞裂解液和生物体液中快速捕获和浓缩蛋白质样品。蛋白质 G Mag Sepharose的配基是经过优化，用来匹配免疫沉淀反应。
IRDye® 800CW Infrared Dyes,IRDye® 800CW 红外染料
IRDye® 680RD Infrared Dyes,IRDye® 680RD 红外染料
IRDye® 680LT Infrared Dyes,IRDye® 680LT 红外染料
IRDye® 750 Infrared Dyes,IRDye® 750 红外染料
IRDye® 800RS NHS Ester,IRDye® 800RS NHS 酯
IRDye® QC-1 NHS Ester,IRDye® QC-1 NHS 酯
IRDye® Phosphoramidites,IRDye® 亚磷酰胺
IRDye Infrared Fluorescent Dyes and near-infrared (NIR) fluorescence imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios.
IRDye Fluorescent Dyes have absorption and emission wavelengths in the NIR spectrum, between 680 and 800 nm. Most of the IRDye reagents are compatible with the Odyssey® DLx, Odyssey XF, Odyssey Sa, Pearl® Imagers, and 4300 DNA Analyzer platforms.
IRDye 红外荧光染料和近红外 (NIR) 荧光成像由于在近红外区域的背景自发荧光低，因此具有更高的信噪比，从而提高了灵敏度。
IRDye 荧光染料在 NIR 光谱中具有 680 至 800 nm 的吸收和发射波长。 大多数 IRDye 试剂与 Odyssey® DLx、Odyssey XF、Odyssey Sa、Pearl® Imagers 和 4300 DNA 分析仪平台兼容。
Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye Infrared Dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amino groups.
IRDye maleimides provide the functionality for labeling molecules that contain free sulfhydryl (-SH) groups with IRDye Infrared Dyes. The reactive group of the IRDye maleimide allows conjugation reactions to be performed very efficiently at physiological pH.
IRDye 马来酰亚胺提供了用 IRDye 红外染料标记含有游离巯基 (-SH) 基团的分子的功能。 IRDye 马来酰亚胺的反应性基团允许偶联反应在生理 pH 值下非常有效地进行。
Assays that use infrared dye conjugates (such as in vivo imaging and cell binding assays) may require a “dye-only” control for potential effects or retention of the dye. The carboxylate dye has no reactive group and cannot be used for labeling.
Carboxylate dye forms can serve as an ideal control for cell-based assays that monitor binding of a dye-labeled agent to validate optical agents for in vivo administration and to evaluate binding specificity. This dye form can also be used to evaluate the behavior and clearance of the dye itself and as a standard to determine the amount of unreacted (“free”) dye after conjugation and purification.
使用红外染料偶联物的测定（例如体内成像和细胞结合测定）可能需要“仅染料”控制以控制染料的潜在影响或保留。 羧酸染料没有反应基团，不能用于标记。
羧酸盐染料形式可以作为基于细胞的测定的理想对照，监测染料标记试剂的结合，以验证用于体内给药的光学试剂并评估结合特异性。 这种染料形式还可用于评估染料本身的行为和清除率，并作为确定结合和纯化后未反应（“游离”）染料量的标准。
IRDye Protein Labeling Kits label antibodies, other proteins, and peptides for applications such as Western blots, In Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. Applications of dye-labeled conjugates vary, depending on the IRDye used for labeling.
IRDye Labeling Kits
Biomolecule labeling continues to be a cornerstone feature of many in vitro and in vivo biological experiments. Click Chemistry has recently emerged as a convenient, versatile, and reliable method for labeling a wide variety of molecules for applications ranging from biomarker isolation to assay development. Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally occurring functional groups such as amines
LI-COR offers DBCO, azide, and alkyne dye forms of IRDye 800CW and IRDye 680RD.
Click Chemistry Dyes
| Dye | Exmax (nm)* | Emmax (nm)* | NHS Ester | Maleimide | Carboxylate | DBCO | Azide | Alkyne | Phosphoramidite | LI-COR Channel |
|---|---|---|---|---|---|---|---|---|---|---|
| IRDye 800CW | 778 | 794 |  |
 |
|
|
|
|
800 nm | |
| IRDye 800RS | 770 | 786 | |
800 nm | ||||||
| IRDye 800 phosphoramidite | 787 | 812 | |
800 nm | ||||||
| IRDye 750 | 766 | 776 | |
|
||||||
| IRDye 700 phosphoramidite | 680 | 697 | |
700 nm | ||||||
| IRDye 680LT** | 680 | 694 | |
|
700 nm | |||||
| IRDye 680RD | 680 | 694 | |
|
|
|
|
|
700 nm |
* Data collected using methanol as the solvent.
** IRDye 680LT dye products should not be used for small animal in vivo imaging or In-Cell Western Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.
LI-COR offers three distinct 700 channel dyes for a variety of applications. Choosing the correct dye is critical when developing an experiment. A summary of each dye and an application table are provided to help you determine which dye is best for your needs.
IRDye 680RD is the near-infrared fluorescent dye of choice for small animal imaging, Western blot, and In-Cell Western Assay applications in the 700nm channel. It has the lowest background compared to LI-COR’s other 700 nm dyes and can be used for the widest variety of applications with little optimization. The NHS ester and maleimide forms of this infrared dye are ideal for labeling proteins, peptides, and antibodies. LI-COR offers a variety of IRDye 680RD dyes, labeling kits and secondary antibodies.
IRDye 680LT is significantly brighter and more photostable than many other 700 nm near-infrared dyes such as Alexa Fluor® 680. It provides superior performance for protein detection applications, including microscopy and Western blot applications. LI-COR offers a variety of IRDye 680LT dyes, labeling kits, and secondary antibodies. When using IRDye 680LT secondary antibodies for Western blot detection, it is critical to follow the recommendations in the pack insert. Dilution optimization may be required to achieve best results.
| Application | IRDye 680RD | IRDye 680LT |
|---|---|---|
| Western Blot | |
|
| In-Cell Western Assay | |
not recommended |
| On-Cell Western Assay | |
not recommended |
| RNAi Screens | |
for Western blots only |
| Protein Array | |
|
| Immunohistochemistry | |
|
| Microscopy | |
|
| 2D Gel Detection | |
|
| Tissue Section Imaging | |
|
| FRET-based Assays | |
|
| Small Animal Imaging | |
not recommended |
IRDye Near-Infrared Dyes are optimized for excitation and detection in the near-infrared spectrum, providing numerous benefits.
LI-COR offers IRDye 800CW that has been manufactured under current good manufacturing practices (cGMP) and is available for conjugation to targeted biomolecules for investigational use in clinical trials. In September of 2007, LI-COR Biosciences announced the successful completion of animal toxicity studies of its IRDye 800CW infrared dye carboxylic acid using a protocol reviewed by the Food and Drug Administration. The completion of the toxicity studies was a key milestone in the development of the IRDye 800CW dye for potential clinical imaging use. The complete report was published in 2010 and is available to the public. In addition, drug master files (DMF) are on record with U.S. and European regulatory authorities.
LI-COR 提供的 IRDye 800CW 是根据当前的良好生产规范 (cGMP) 制造的，可用于与靶向生物分子结合，用于临床试验的研究用途。 2007 年 9 月，LI-COR Biosciences 宣布使用食品和药物管理局审查的协议成功完成了 IRDye 800CW 红外染料羧酸的动物毒性研究。 毒性研究的完成是 IRDye 800CW 染料开发用于潜在临床成像的关键里程碑。 完整的报告于 2010 年发布，可供公众查阅。 此外，药物主文件 (DMF) 已在美国和欧洲监管机构备案。
LI-COR IRDye Infrared Fluorescent Dyes are used for protein and cellular assays, microscopy, and in vivo molecular imaging in animals for research purposes. IRDye 800CW is cited in a wide variety of published non-clinical research for labeling nucleic acids, antibodies, proteins, and peptides where there is a need for high signal, low background imaging. With an 800nm emission wavelength, the IRDye 800CW dye is spectrally ideal for animal imaging research.
While the IRDye 800CW dye has successfully completed toxicity studies, it has not been studied for diagnostic or therapeutic use in humans, and has not been approved by the Food and Drug Administration for this use. LI-COR is exploring options for additional studies as next steps in the Food and Drug Administration approval process leading toward clinical use.
LI-COR IRDye 红外荧光染料用于研究目的的动物蛋白质和细胞分析、显微镜检查和体内分子成像。 IRDye 800CW 在各种已发表的非临床研究中被引用，用于标记需要高信号、低背景成像的核酸、抗体、蛋白质和肽。 IRDye 800CW 染料的发射波长为 800nm，是动物成像研究的理想光谱。
虽然 IRDye 800CW 染料已成功完成毒性研究，但尚未研究用于人类的诊断或治疗用途，也未获得美国食品和药物管理局的批准用于此用途。 LI-COR 正在探索其他研究的选择，作为食品和药物管理局批准程序的下一步，以实现临床使用。
IRDye 800CW conjugated probes and agents have propelled more than 20 Phase I and Phase II clinical trials, To learn more about LI-COR infrared dye conjugates being used in clinical trials, visit Probe Development Clinical Applications.
To discuss purchase quantities over 1 gram or commercial development rights using IRDye infrared dyes, please contact Business Development.
| Application | IRDye 680RD | IRDye 680LT |
|---|---|---|
| Western Blot | |
|
| In-Cell Western Assay | |
not recommended |
| On-Cell Western Assay | |
not recommended |
| RNAi Screens | |
for Western blots only |
| Protein Array | |
|
| Immunohistochemistry | |
|
| Microscopy | |
|
| 2D Gel Detection | |
|
| Tissue Section Imaging | |
|
| FRET-based Assays | |
|
| Small Animal Imaging | |
not recommended |
Reagents > 96-Well Plate for In-Cell Western™ Assays
Sterile cell culture plates optimized for use for the In-Cell Western Assay. These plates include a lid and have a clear, polystyrene bottom with black walls.
无菌细胞培养板优化用于 In-Cell Western Assay。 这些盘子包括一个盖子，并有一个透明的聚苯乙烯底部和黑色的墙壁。
货号：28967066描述：Protein G Mag Sepharose Xtra 2*1mL
Protein G Mag Sepharose Xtra磁珠结合成熟的亲和层析方法 和磁珠平台，提高样品制备过程中的简易性和易用性。Mag Sepharose磁珠特征包括密度高、黑色和可可视性，操作非常简单。磁珠更低的拖尾效应和聚集沉淀意味着缓冲液不需要使用表面活性剂。
结合MagRack 6，用于微量离心管的分离工具，可以并行处理六份样品。这样，你可以在高通量条件下轻松并行筛选大量样品。Protein G Mag Sepharose Xtra提供两种版本，2 ?— 1 ml包装，可使用20次提纯，5 ?— 1 ml包装，可使用50次提纯。产品将磁包含在琼脂糖中制备而成，配基是天然蛋白质G。蛋白质G有较高亲和力，适合于各种生物IgG抗体的Fc区域。
Protein G Mag Sepharose是Mag Sepharose 平台的另一种产品，可以用于免疫沉淀反应技术，用于从细胞裂解液和生物体液中快速捕获和浓缩蛋白质样品。蛋白质 G Mag Sepharose的配基是经过优化，用来匹配免疫沉淀反应。
IRDye® 800CW Protein Labeling Kits,IRDye® 800CW 蛋白质标记试剂盒
IRDye® 680RD Protein Labeling Kits,IRDye® 680RD 蛋白质标记试剂盒
IRDye® 680LT Protein Labeling Kits,IRDye® 680LT 蛋白质标记试剂盒
IRDye® 650 Protein Labeling Kits,IRDye® 650 蛋白质标记试剂盒
IRDye® Protein Labeling Kits label antibodies and other proteins for applications such as Western blots, In Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. Applications vary depending on the IRDye infrared dye selected for labeling. Please refer to specific dye page to find out which applications apply to each IRDye NHS ester.
IRDye® 蛋白质标记试剂盒可标记抗体和其他蛋白质，用于蛋白质印迹、In Cell Western™ 检测、体内成像以及整个器官或组织切片检测等应用。 应用因选择用于标记的 IRDye 红外染料而异。 请参阅特定染料页面，了解每种 IRDye NHS 酯的应用。
IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary.
IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water-soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.
For more information, refer to the IRDye Peptide Labeling Application Guide.
Accessories > Alcohol Prep Pad Refills
Alcohol Prep pads are part of the Dye Decontamination Kit (P/N 9300-840). You can purchase additional pads separately.
酒精准备垫是染料去污套件 (P/N 9300-840) 的一部分。 您可以单独购买额外的护垫。
货号：28944008描述：Protein G Mag Sepharose 1*500ul
Protein G Mag Sepharose磁珠适用于通过免疫沉淀或pull-down方法进行目标蛋白的富集。磁珠以琼脂糖凝胶为基架，天然蛋白G作为配体。蛋白G对不同种属的IgG抗体的Fc区具有高度亲和性
有两种可用于蛋白质富集的实验方法：交联方法和经典方法。这些优化的洗脱条件使得电泳和MS分析简单可行。
蛋白A Mag Sepharose将被充分证明的富集方法与磁珠平台相结合，适合于小规模样品制备。MagRack 6是一个分离工具，可与6个离心管联用，实现最多可并行处理六个样品。
IRDye® 800CW Secondary Antibodies,IRDye® 800CW 二抗
IRDye 800CW Rabbit anti-HRP Secondary Antibody,IRDye 800CW 兔抗 HRP 二抗
IRDye® 680RD Secondary Antibodies,IRDye® 680RD 二抗
IRDye® 680LT Secondary Antibodies,IRDye® 680LT 二抗
Accessories > Black Western Blot Incubation Boxes
Black blot boxes are suitable for incubating near-infrared fluorescent and chemiluminescent Western blots or protein gels.
黑色印迹盒适用于孵育近红外荧光和化学发光蛋白质印迹或蛋白质凝胶。
Four sizes are available in single, five- and ten-pack quantities.
| Size | Length | Width | Height |
|---|---|---|---|
| Small | 7.2 cm | 4.9 cm | 3.0 cm |
| Medium | 8.9 cm | 6.5 cm | 2.8 cm |
| Large | 11.5 cm | 8.8 cm | 2.8 cm |
| X-Large | 15.2 cm | 10.1 cm | 3.1 cm |
货号：28951379描述：Protein G Mag Sepharose 4*500ul
Protein G Mag Sepharose磁珠适用于通过免疫沉淀或pull-down方法进行目标蛋白的富集。磁珠以琼脂糖凝胶为基架，天然蛋白G作为配体。蛋白G对不同种属的IgG抗体的Fc区具有高度亲和性
有两种可用于蛋白质富集的实验方法：交联方法和经典方法。这些优化的洗脱条件使得电泳和MS分析简单可行。
蛋白A Mag Sepharose将被充分证明的富集方法与磁珠平台相结合，适合于小规模样品制备。MagRack 6是一个分离工具，可与6个离心管联用，实现最多可并行处理六个样品。
IRDye® 800CW Streptavidin,IRDye® 800CW 链霉亲和素
IRDye® 680RD Streptavidin,IRDye® 680RD 链霉亲和素
IRDye® 680LT Streptavidin,IRDye® 680LT 链霉亲和素
VRDye™ 549 Streptavidin,VRDye™ 549 链霉亲和素
VRDye™ 490 Streptavidin
Streptavidin binds to biotin very strongly and is used to detect biotinylated proteins.
Fluorescent dye-labeled streptavidins can be used as a secondary detection reagent for microscopy, protein arrays, In-Gel Westerns, and Western blotting.
VRDye™ 490 链霉亲和素
链霉亲和素与生物素的结合非常强烈，可用于检测生物素化的蛋白质。
荧光染料标记的链霉亲和素可用作显微镜检查、蛋白质阵列、In-Gel Westerns 和蛋白质印迹的二级检测试剂。
All streptavidins are supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. For IRDye® Streptavidin, a final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications. For VRDye™ Streptavidin, a concentration of 1:2,000 to 1:5,000 is usually satisfactory for Western blotting. However, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
所有链霉亲和素均以缓冲液形式提供，缓冲液中含有 10 mM 磷酸盐、183 mM NaCl、2.7 nM KCl，pH 7.4，叠氮化钠 0.005% (w/v) 作为防腐剂。
要使用，请在使用前短暂离心以消除可能在溶液中形成的聚集体。 对于 IRDye® Streptavidin，0.2 至 1.0 µg/ml（1:1,000 至 1:5,000）的最终浓度通常对于大多数应用来说是令人满意的。 对于 VRDye™ Streptavidin，1:2,000 至 1:5,000 的浓度通常可以满足蛋白质印迹的要求。 但是，可能需要根据经验确定适当的稀释度。
对于基于膜的应用和 In-Gel Western，建议在检测孵育步骤中添加 SDS（0.02% 至 0.1% 最终浓度）和 Tween® 20（0.1% 至 0.2% 最终浓度）以减少非 特定背景染色。
Reagents > CellTag™ 520 Stain for In-Cell Western™ Assays
CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.
The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.
CellTag 520 Stain is detected in the 520 nm channel. CellTag 520 Stain is applied to the cells during incubation with an IRDye® Secondary Antibodies (e.g., IRDye 680RD or IRDye 800CW), and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
CellTag 520 Stain 是一种可见的荧光、非特异性细胞染料，可为 In-Cell Western™ Assay 应用提供准确的细胞数标准化。
染色剂在透化细胞的细胞核和细胞质中积累，并在广泛的细胞类型和细胞数量中提供线性荧光信号。
在 520 nm 通道中检测到 CellTag 520 Stain。 CellTag 520 Stain 在与 IRDye® 二抗（例如 IRDye 680RD 或 IRDye 800CW）一起孵育期间应用于细胞，能够以比蛋白质印迹高得多的通量准确测量靶蛋白水平。
货号：28967062描述：Protein A Mag Sepharose Xtra 5*1mL
Protein A Mag Sepharose Xtra磁珠设计用于单克隆和多克隆抗体从血清和细胞上清液中的快速、小规模提纯和筛选应用。高载量， >27 mg 人体IgG/ml，允许高效捕获高产率和高度纯化的抗体。每次提纯生产可以获取大约350g 提纯的人体IgG。
稳健、可重复性高的抗体并行筛选
结合MagRack 6，用于微量离心管中磁珠处理的分离工具，可以并行处理六份样品。使用一台机器人装置，你可以在高通量条件下轻松并行筛选大量样品
PBS Blocking Buffer and PVDF Kits,PBS 封闭缓冲液和 PVDF 试剂盒
TBS Blocking Buffer and PVDF Kits,TBS 封闭缓冲液和 PVDF 试剂盒
Odyssey® Nitrocellulose Membranes,Odyssey® 硝酸纤维素膜
4X Protein Sample Loading Buffer and PVDF Membrane Kit,4X 蛋白质上样缓冲液和 PVDF 膜试剂盒
A low-background membrane is essential for infrared Western blot success. Background can be attributed to membrane autofluorescence or to detection of antibody non-specifically binding to the membrane.
低背景膜对于红外蛋白质印迹的成功至关重要。 背景可归因于膜自发荧光或检测到与膜非特异性结合的抗体。
LI-COR has evaluated many different membranes for Western blotting; examples of membrane performance can be seen in the figure below. There is typically more variability in PVDF membrane performance than for nitrocellulose membranes.
LI-COR offers high-quality, prescreened Immobilon®-FL PVDF, without lot-to-lot variability, packaged in affordable kits with the Intercept™ Blocking Buffer (PBS or TBS), or with 4X Protein Sample Loading Buffer. LI-COR also offers nitrocellulose membranes in both sheets and rolls.
LI-COR 已经评估了许多不同的蛋白质印迹膜。 膜性能示例如下图所示。 PVDF 膜的性能通常比硝酸纤维素膜具有更大的可变性。
LI-COR 提供高质量、预筛选的 Immobilon®-FL PVDF，无批次间差异，采用经济实惠的试剂盒包装，带有 Intercept™ 封闭缓冲液（PBS 或 TBS）或 4X 蛋白质上样缓冲液。 LI-COR 还提供片状和卷状硝酸纤维素膜。
| Product | P/N | What’s Included |
|---|---|---|
| Odyssey Nitrocellulose Membranes | 926-31090 | 0.22 µm; ten 7 cm × 8.5 cm sheets |
| Odyssey Nitrocellulose Membranes | 926-31092 | 0.22 µm; 30 cm × 3 m roll |
| PBS Blocking Buffer (500 mL) and PVDF Kit | 926-32098 | 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (PBS) Blocking Buffer |
| TBS Blocking Buffer (500 mL) and PVDF Kit | 926-32099 | 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (TBS) Blocking Buffer |
| 4X Protein Sample Loading Buffer and PVDF Membrane Kit | 926-31097 | 0.45 µm; 26.5 cm × 3.75 m roll + 15 mL 4X Protein Sample Loading Buffer |
| PBS Blocking Buffer (10 x 500 mL) and PVDF Kit | 829-32080 | 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (PBS) Blocking Buffer |
| TBS Blocking Buffer (10 x 500 mL) and PVDF Kit | 926-32100 | 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (TBS) Blocking Buffer |
Reagents > CellTag™ 700 Stain for In-Cell Western™ Assays
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.
The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.
CellTag 700 Stain is detected in the 700 nm channel of Odyssey® CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
CellTag 700 Stain 是一种近红外荧光非特异性细胞染料，可为 In-Cell Western™ Assay 应用提供准确的细胞数标准化。
染色剂在透化细胞的细胞核和细胞质中积累，并在广泛的细胞类型和细胞数量中提供线性荧光信号。
在 Odyssey® CLx、Classic 和 Sa 成像系统的 700 nm 通道中检测到 CellTag 700 Stain。 CellTag 700 Stain 在与 IRDye® 800CW 二抗孵育期间应用于细胞，可准确测量靶蛋白水平，其通量远高于蛋白质印迹法。
货号：28944006描述：Protein A Mag Sepharose 1*500ul
Protein A Mag Sepharose磁珠适用于通过免疫沉淀或pull-down方法进行目标蛋白的富集。磁珠以琼脂糖凝胶为基架，天然蛋白A作为配体。蛋白A对不同种属的IgG抗体的Fc区具有高度亲和性
有两种可用于蛋白质富集的实验方法：交联方法和经典方法。这些优化的洗脱条件使得电泳和MS分析简单可行。
蛋白A Mag Sepharose将被充分证明的富集方法与磁珠平台相结合，适合于小规模样品制备。MagRack 6是一个分离工具，可与6个离心管联用，实现最多可并行处理六个样品。
Odyssey® 28KD Loading Indicator – 800 nm
Odyssey® 28 KD Loading Indicator – 700 nm
Odyssey Loading Indicators (OLI) provide a simple, convenient method to evaluate the consistency of sample loading volume across gel lanes, as well as the uniformity of Western blot transfer.
It is essential to have consistent sample loading between gel lanes for comparative and quantitative Western blot analysis. By adding equal amounts of an Odyssey Loading Indicator, you can determine if loading was consistent or if a pipetting error occurred.
Odyssey Loading Indicators (OLI) 提供了一种简单方便的方法来评估凝胶通道上样品上样量的一致性，以及蛋白质印迹转移的均匀性。
对于比较和定量的蛋白质印迹分析，凝胶泳道之间必须有一致的上样量。 通过添加等量的 Odyssey 上样指示剂，您可以确定上样是否一致或是否发生移液错误。
Note: Accurate normalization relies on an internal loading control, an endogenous protein used as an indicator of sample concentration. Since the OLI is an exogenous protein, it functions as an external loading control, and cannot be used for normalization. However, you can use OLI to help check if your internal loading control is stably expressed.
Visit our Western blot Normalization page for more information on Western blot normalization strategies.
If you’re using a housekeeping protein to normalize Western blot data, you must validate that its expression is constant across all samples and is unaffected by experimental conditions. As an exogenous protein, the Odyssey Loading Indicator is not subject to up- or down-regulation as a function of experimental sample treatment. A stable signal for an OLI across several lanes indicates that any signal variation for the housekeeping protein was not caused by inconsistent sample loading.
如果您使用看家蛋白来标准化蛋白质印迹数据，您必须验证其在所有样品中的表达是恒定的，并且不受实验条件的影响。 作为外源性蛋白质，Odyssey Loading Indicator 不受实验样品处理的上调或下调影响。 跨多个泳道的 OLI 信号稳定表明管家蛋白的任何信号变化都不是由不一致的样品加载引起的。
“‘House-keeping’ proteins should not be used for normalization without evidence that experimental manipulations do not affect their expression.”
Figure 2. Assessing stable expression of tubulin and COX IV with OLI. The housekeeping protein signal intensity was plotted, along with signal intensities of OLI and Revert 700 Total Protein Stain. Both (A) tubulin and (B) COX IV signals varied in response to treatment, while Revert 700 Total Protein Stain signals and OLI signals remained constant. These quantitative data indicate the expression of both housekeeping proteins was affected by Etoposide treatment.
图 2. 使用 OLI 评估微管蛋白和 COX IV 的稳定表达。 绘制管家蛋白信号强度以及 OLI 和 Revert 700 总蛋白染色剂的信号强度。 (A) 微管蛋白和 (B) COX IV 信号均随治疗而变化，而 Revert 700 Total Protein Stain 信号和 OLI 信号保持不变。 这些定量数据表明两种看家蛋白的表达都受到依托泊苷处理的影响。
Reagents > CellVue® Burgundy Fluorescent Cell Labeling Kit
The CellVue Burgundy cell labeling kit uses proprietary membrane labeling technology to stably incorporate CellVue Burgundy, a fluorescent dye with long aliphatic tails, into lipid regions of the cell membrane1. The labeling buffer provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The labeling efficiency of staining is dependent upon the cell type being labeled and the membranes of the cells2, 3.
CellVue Burgundy 细胞标记试剂盒使用专有的膜标记技术，将具有长脂肪族尾巴的荧光染料 CellVue Burgundy 稳定地结合到细胞膜的脂质区域1。 试剂盒随附的标记缓冲液（稀释剂 C）是一种等渗水溶液，不含生理盐或缓冲液、去污剂或有机溶剂，旨在保持细胞活力，同时最大限度地提高染料溶解度和染色效率。 染色的标记效率取决于被标记的细胞类型和细胞膜2、3。
CellVue products have been reported to be useful for short-term in vitro cell proliferation studies4, cell tracking in isolated organ preparation applications5, and cell tracking using the Pearl® Small Animal Imaging System or Odyssey® CLx or Odyssey Classic Infrared Imaging System6.
The excitation and emission properties of CellVue Burgundy dye are compatible with a range of commercially available near-infrared fluorescent plate readers, flow cytometers, in vitro and in vivo fluorescent imaging systems, and confocal microscopes.
CellVue is a registered trademark of PTI Research, Inc, used under license. CellVue products are sold under license from PTI Research, Inc.. US Patent # 8,029,767B2.
CellVue 是 PTI Research, Inc 的注册商标，经许可使用。 CellVue 产品在 PTI Research, Inc. 的许可下销售。美国专利 # 8,029,767B2。
货号：28967056描述：Protein A Mag Sepharose Xtra 2*1mL
Protein A Mag Sepharose Xtra磁珠设计用于单克隆和多克隆抗体从血清和细胞上清液中的快速、小规模提纯和筛选应用。高载量， >27 mg 人体IgG/ml，允许高效捕获高产率和高度纯化的抗体。每次提纯生产可以获取大约350 ??g 提纯的人体IgG。
稳健、可重复性高的抗体并行筛选
结合MagRack 6，用于微量离心管中磁珠处理的分离工具，可以并行处理六份样品。使用一台机器人装置，你可以在高通量条件下轻松并行筛选大量样品
IRDye® 800CW 2-DG Optical Probe
IRDye® 800CW EGF Optical Probe
IRDye® 800CW RGD Optical Probe
IRDye® 800CW BoneTag™ Optical Probe,IRDye® 800CW BoneTag™ 光学探头
IRDye® 680RD BoneTag™ Optical Probe
IRDye® 800CW PEG Fluorescent Contrast Agent
Every LI-COR BrightSite IRDye in vivo imaging agent has been carefully validated with cultured cell assays, microscopy, in vivo imaging of animal models, and histology to ensure high affinity and specificity.
BrightSite IRDye Small Animal Imaging Agents are:
IRDye 800CW absorption/emission near 800 nm matches near-infrared absorption minima for bodily fluids and tissues, resulting in excellent tissue penetration, making it ideal for in vivo imaging.
LI-COR also offers the PSVue® 794 Reagent Kit which can be used to detect apoptotic and necrotic cells, bacteria and other anionic membranes.
LI-COR 还提供 PSVue® 794 试剂盒，可用于检测凋亡和坏死细胞、细菌和其他阴离子膜。
CellVue® fluorescent imaging kits use proprietary labeling technology to stably incorporate fluorescent dyes containing long aliphatic hydrocarbon tails into lipid membranes.1 They are useful for researchers working in all aspects of science and technology where fluorescently-labeled cells and/or tissues are required. CellVue dyes also provide researchers with valuable tools for many in vivo and in vitro cell studies using fluorescent membrane labels.
CellVue® 荧光成像试剂盒使用专有的标记技术，将含有长脂肪烃尾的荧光染料稳定地掺入脂质膜中。1 它们适用于在需要荧光标记的细胞和/或组织的科学和技术的各个方面工作的研究人员。 CellVue 染料还为研究人员提供了许多使用荧光膜标记进行体内和体外细胞研究的宝贵工具。
CellVue dyes consist of long aliphatic hydrocarbon tails linked to a polar fluorescent chromophore.
Molar extinction coefficient characteristics of water, hemoglobin and oxygenated hemoglobin (400-1000 nm). IRDye infrared dyes have ideal excitation/emission wavelengths for in vivo imaging.
| Application | IRDye EGF | IRDye RGD | IRDye 2-DG | IRDye PEG | IRDye BoneTag | CellVue | PSVue |
|---|---|---|---|---|---|---|---|
| Tumor Imaging | |
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| Inflammation/Arthritis | |
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| Apoptosis | |
Reagents > CellVue® NIR815 Fluorescent Cell Labeling Kit
The CellVue NIR815 cell labeling kit uses proprietary membrane labeling technology to stably incorporate CellVue NIR815, a fluorescent dye with long aliphatic tails, into lipid regions of the cell membrane1. The labeling buffer provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The labeling efficiency of staining is dependent upon the cell type being labeled and the membranes of the cells2, 3.
CellVue NIR815 细胞标记试剂盒使用专有的膜标记技术将具有长脂肪族尾巴的荧光染料 CellVue NIR815 稳定地结合到细胞膜的脂质区域1。 试剂盒随附的标记缓冲液（稀释剂 C）是一种等渗水溶液，不含生理盐或缓冲液、去污剂或有机溶剂，旨在保持细胞活力，同时最大限度地提高染料溶解度和染色效率。 染色的标记效率取决于被标记的细胞类型和细胞膜2、3。
CellVue NIR815 dye has been reported to be useful for short-term in vitro cell proliferation studies4, cell tracking in isolated organ preparation applications5, and cell tracking using the Pearl® Small Animal Imaging System or Odyssey® CLx Imager or Odyssey Classic Infrared Imaging System6.
The excitation and emission properties of CellVue NIR815 dye are compatible with a range of commercially available near-infrared fluorescent plate readers, flow cytometers, in vitro and in vivo fluorescent imaging systems, and confocal microscopes.
CellVue NIR815 染料的激发和发射特性与一系列市售的近红外荧光读板机、流式细胞仪、体外和体内荧光成像系统以及共聚焦显微镜兼容。
CellVue is a registered trademark of PTI Research, Inc, used under license. CellVue products are sold under license from PTI Research, Inc.. US Patent # 8,029,767B2.
货号：28951378描述：Protein A Mag Sepharose 4*500ul
Protein A Mag Sepharose磁珠适用于通过免疫沉淀或pull-down方法进行目标蛋白的富集。磁珠以琼脂糖凝胶为基架，天然蛋白A作为配体。蛋白A对不同种属的IgG抗体的Fc区具有高度亲和性
有两种可用于蛋白质富集的实验方法：交联方法和经典方法。这些优化的洗脱条件使得电泳和MS分析简单可行。
蛋白A Mag Sepharose将被充分证明的富集方法与磁珠平台相结合，适合于小规模样品制备。MagRack 6是一个分离工具，可与6个离心管联用，实现最多可并行处理六个样品。
β-Actin Rabbit Monoclonal Antibody,β-肌动蛋白兔单克隆抗体
β-Actin Mouse Monoclonal Antibody,β-肌动蛋白小鼠单克隆抗体
COX IV Rabbit Primary Antibody,COX IV 兔一抗
GAPDH Rabbit Monoclonal Antibody,GAPDH 兔单克隆抗体
Histone H3 Mouse Monoclonal Antibody,组蛋白 H3 小鼠单克隆抗体
Histone H3 Rabbit Monoclonal Antibody
α-Tubulin Mouse Monoclonal Antibody
β-Tubulin Rabbit Polyclonal Antibody,β-微管蛋白兔多克隆抗体
Vinculin Rabbit Monoclonal Antibody,Vinculin 兔单克隆抗体
When performing quantitative Western blots, internal loading controls and normalization are essential for reliable, precise comparison of protein expression. LI-COR offers several primary antibodies which can be used for two-color normalization when performing multiplex Western blots or for cell-based assay normalization, such as for In-Cell Western™ Assays.
在进行定量蛋白质印迹时，内部上样对照和标准化对于可靠、精确地比较蛋白质表达至关重要。 LI-COR 提供了多种一抗，可用于在进行多重蛋白质印迹或基于细胞的测定标准化（例如 In-Cell Western™ 测定）时进行双色标准化。
For Western blots, lysate preparation, sample loading, and membrane transfer introduce unavoidable variation. After validation, housekeeping proteins (HKP) can be used for normalization of protein levels.
Read more about using a housekeeping protein as an internal loading control.
When using an HKP as your normalization strategy, it’s important to validate your HKP for each experiment to ensure its expression is stable. Many factors can influence expression including tissue, treatment, and cell density.
“‘House-keeping’ proteins should not be used for normalization without evidence that experimental manipulations do not affect their expression.”
The Housekeeping Protein Validation Protocol can help you validate that your HKP expression is not changing with treatment. The Housekeeping Protein Normalization Protocol will guide you through the right experimental steps and calculations for normalization using an HKP. Validating your HKP is the only way to know that expression is not affected by the conditions of your experiment
管家蛋白质验证方案可以帮助您验证您的 HKP 表达不会随着治疗而改变。 管家蛋白质标准化方案将指导您完成正确的实验步骤和使用 HKP 进行标准化的计算。 验证您的 HKP 是了解表达式不受实验条件影响的唯一方法
For In-Cell Western Assays, a second protein target (such as actin, tubulin, COX IV, or GAPDH) can be used for normalization. Abundance of the normalization target must be unaffected by the cell treatments used.
Learn more about ICW assay normalization methods.
The table below has a few examples of some common housekeeping proteins, their molecular weights, and a few considerations. This list is not complete, and any housekeeping protein you choose should be fully validated for your system under study.
Sign up to review the science, best practices and mechanics of normalization strategies. Housekeeping proteins can be used as a strategy for Western blot normalization with the right experimental design. Find out what publishers need from you when using the housekeeping protein normalization strategy.
Lambda U® online learning portal is an online, on-demand resources can help ensure that you are getting the very best Western blot data.
注册以查看标准化策略的科学、最佳实践和机制。 通过正确的实验设计，管家蛋白可用作蛋白质印迹标准化的策略。 了解出版商在使用管家蛋白质标准化策略时需要您提供什么。
Lambda U® 在线学习门户是一个在线按需资源，可帮助确保您获得最佳的蛋白质印迹数据。
Sign up to review the science, best practices and mechanics of normalization strategies. Housekeeping proteins can be used as a strategy for Western blot normalization with the right experimental design. Find out what publishers need from you when using the housekeeping protein normalization strategy.
Lambda U® online learning portal is an online, on-demand resources can help ensure that you are getting the very best Western blot data.
货号：17371220描述：His Mag Sepharose excel 2*1mL
His Mag Sepharose excel是一种磁性固定化金属离子亲和层析(IMAC)介质，预先带有镍离子，并稳定结合在chelating配体上。该介质可实现小规模捕获、提纯和可重复筛选从真核细胞—比如昆虫细胞或中国仓鼠卵巢(CHO)细胞表达或分泌到细胞培养上清中的组氨酸标记蛋白。Mag Sepharose产品在小规模实验中具有优良的性能。高密度琼脂糖磁珠允许被磁性装置快速捕获，而微球的可见性确保能够在提纯期间，可靠地收集结合的组氨酸标记蛋白。该基质基于包含磁性物质的琼脂糖介质制备。
His Mag Sepharose提供三种不同包装尺寸规格的10%混悬液(2 x 1 ml, 5 x 1 ml, and 10 x 1 ml)，并且可以和不同的试管、磁性架结合使用，比如MagRack 6 – 适用于1.5 ml以内的试管，或MagRack Maxi-适用于50 ml以内的试管。根据推荐的说明书，1 ml混悬液足够用于5次反应。产品交付时装载在试管中，包含1 ml 10% (v/v)混悬液，其中包含100μl的磁珠。
Reagents > Chameleon® 700 Pre-stained Protein Ladder
The Chameleon 700 Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for both visible and 700 channel near-infrared detection.
Chameleon 700 Pre-stained Protein Ladder 为可见光和 700 通道近红外检测提供多色预染色蛋白。
Not sure which protein marker to choose? Visit How to Choose the Right Protein Ladder.
Chameleon® Vue Pre-stained Protein Ladder,Chameleon® Vue 预染蛋白阶梯
Chameleon® NIR Prestained Protein Ladders,Chameleon® NIR 预染蛋白阶梯
Odyssey® One-Color Protein Molecular Weight Marker,Odyssey® 单色蛋白质分子量标记
WesternSure® Pre-stained Protein Ladder
A protein marker (also called a protein molecular weight marker, a protein MW marker, or a protein ladder) is used to estimate the size of proteins resolved by gel electrophoresis.
| Visible By Eye | Detected With Chemiluminescence | Visible In 700 nm Channel | Visible In 800 nm Channel | |
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| WesternSure® Ladder | |
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| Odyssey® Protein MW Marker | |
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| Chameleon® Vue | |
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| Chameleon Duo | |
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| Chameleon Kit | |
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| Chameleon 700 | |
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| Chameleon 800 | |
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* Chameleon Vue can be detected with chemiluminescence when used with the WesternSure Pen, P/N 926-91000
Sign up to review the science, best practices and mechanics of gel preparation for free, including the use of molecular weight markers at Lambda U® online learning portal.
These online, on-demand resources can help ensure that you are getting the very best Western blot data.
Sign Up
Sign up to review the science, best practices and mechanics of gel preparation for free, including the use of molecular weight markers at Lambda U® online learning portal.
These online, on-demand resources can help ensure that you are getting the very best Western blot data.
Reagents > Chameleon® 800 Pre-stained Protein Ladder
The Chameleon 800 Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for both visible and 800 channel near-infrared detection.
Chameleon 800 Pre-stained Protein Ladder 为可见光和 800 通道近红外检测提供多色预染色蛋白。
Not sure which protein marker to choose? Visit How to Choose the Right Protein Ladder.
货号：28967390描述：His Mag Sepharose Ni 5*1mL
His Mag Sepharose Ni磁珠产品用于不同来源组氨酸融合蛋白质的快速、小规模提纯和筛选工艺。Mag Sepharose产品对于在小规模实验方面具有优良性能。磁珠的高密度允许被磁性装置快速捕获，同时磁珠的可视性确保在提纯过程中可靠收集被结合的组氨酸融合蛋白
该产品基于融合有磁性物质和镍固化的IMAC Sepharose介质进行制备
结合MagRack 6，用于微量离心管的分离工具，可以并行处理六份样品
4X Protein Sample Loading Buffer,4X 蛋白质上样缓冲液
4X Protein Sample Loading Buffer and PVDF Membrane Kit,4X 蛋白质上样缓冲液和 PVDF 膜试剂盒
4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange loading buffer is recommended for use with Odyssey® Imaging Systems as it does not fluoresce in the 700nm channel the way blue loading buffers do. The buffer is optimized for use with SDS-PAGE and Tris-Glycine-SDS running buffer.
4X 蛋白质上样缓冲液经过优化，可用作蛋白质凝胶电泳的上样缓冲液。 建议将此橙色上样缓冲液与 Odyssey® Imaging Systems 一起使用，因为它不会像蓝色上样缓冲液那样在 700nm 通道中发出荧光。 该缓冲液经过优化，可与 SDS-PAGE 和 Tris-Glycine-SDS 运行缓冲液一起使用。
The 4X Protein Sample Loading Buffer and PVDF Membrane Kit comes with 1 roll of Immobilon®-FL PVDF Membrane and 1 bottle of 4X Protein Sample Loading Buffer, 15 mL.
4X 蛋白质上样缓冲液和 PVDF 膜试剂盒随附 1 卷 Immobilon®-FL PVDF 膜和 1 瓶 15 mL 4X 蛋白质上样缓冲液。