描述
特定丝氨酸或苏氨酸残基的磷酸化是调节大多数蛋白质活性的重要翻译后修饰。多种细胞信号通路的刺激会激活介导这些蛋白质修饰的受体和非受体 ser/thr 激酶。可以检测磷酸丝氨酸或磷酸苏氨酸残基的抗体是表征各种磷酸化蛋白质翻译后状态变化的绝佳工具。对感兴趣的蛋白质进行免疫沉淀,然后使用抗磷酸丝氨酸抗体检测磷酸丝氨酸或磷酸苏氨酸,通常用于将磷酸化状态的变化与蛋白质活性的变化相关联。
该抗体被交叉吸附到未磷酸化的肽上,然后使用磷酸丝氨酸和磷酸苏氨酸肽的混合物(无载体)亲和纯化。该抗体通过蛋白质印迹、免疫细胞化学和 ELISA 检测许多丝氨酸或苏氨酸磷酸化蛋白质。
Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C.
Product References:
Xue, J. et al. (2019) J Integr Plant Biol. May 14.12824. (WB: Arabidopsis)
Xiao, N. et al. (2019) FASEB J. 33(1):163-174. (IP: Hek293 cells)
Unger, A. et al. (2017) Acta Neuropathol Commun. 5(1):72. (WB: mouse skeletal muscle)
Liu, J. et al. (2016) Cell. 167(4):10521066.e18. (WB: HEK293 transfectants)
Tsai, C.F. et al. (2015) J Agric Food Chem. 9;63(48):10388 (WB: Huh7 cells)
Kommaddi, R.P. et al. (2015) J Biol Chem. 3;290(14):8888 (WB: COS-7 cells)
Kim, SY et al. (2015) Stem Cells Dev. 24(6):686. (WB: mouse stem cells)
Sroyraya, M. (2013) Microc Res Tech 76(1):102. (WB: Swimming Crab)
Kim, S. et al. (2013) Diabetes. 62(2):471. (WB & IP: mouse adipocytes)
Hummel, S. et al. (2012) Appl Environ Microbiol. 78(4):1140. (WB: human colonic adenocarcinoma epithelial cells T84)
Laluk, K. et al. (2011) Plant Cell 23(8):2831. (WB: Arabidopsis BIK1)