分类：lonza

Product Overview

Glucose Stimulated Insulin Release Testing for Pancreatic Islets

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Endothelial cells are the primary barrier cells that line blood vessels and sinusoids of the liver. Human Liver-derived Endothelial Cells (HLECs) are isolated and enriched from liver tissue, then cryopreserved for use in a wide range of research models.

HLECs are characterized for markers of endothelial cells, including CD31, vWF, and LYVE-1 as shown by flow cytometry and maintain appropriate endothelial morphology over limited serial passaging.  Human liver-derived endothelial cells are derived from whole livers leading to a mixed population of vascular endothelial cells and sinusoidal endothelial cells.  

The recommended medium for HLECs is EGM^TM 2 which is optimized for endothelial cell health and growth.

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Leukapheresed CD19+/IgD+ B cells are negatively selected from mononucleated cells using immunomagnetic isolation system to deplete non-naive B cells. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Manufactured by AllCells®

Instructions

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Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Product Overview

Endothelial cells are the primary barrier cells that line blood vessels and sinusoids of the liver. Human Liver-derived Endothelial Cells (HLECs) are isolated and enriched from liver tissue, then cryopreserved for use in a wide range of research models. A unique subtype of LECs, liver sinusoidal endothelial cells, are defined as a subset of cells found at the interface between the liver sinusoids and hepatocytes.

HLECs derived from whole livers lead to a mixed population of vascular endothelial cells and sinusoidal endothelial cells. HLECs characterized for markers of endothelial cells, including CD31 and vWF maintain appropriate endothelial morphology over limited serial passaging. Measurement of uptake of acetylated LDL to indicate percentage of the sinusoidal endothelial cell population. The recommended medium for HLECs is EGMTM 2 which is optimized for endothelial cell health and growth.

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Product Overview

Kupffer cells are the resident macrophages in the liver and are part of the first line of defense against bacteria and toxins in the gut. Kupffer cells exhibit typical macrophage morphology and can be activated to produce inflammatory cytokines, growth factors and reactive oxygen species.

In vivo, prolonged activation of Kupffer cells are associated with many diseases of the liver including Non-alcoholic Fatty Liver Disease (NAFLD), Non Alcoholic Steato Hepatitis (NASH), and liver damage associated with certain bioactivated chemicals. Human Cryopreserved Kupffer cells are isolated and enriched from dissociated human liver tissue and then cryopreserved as passage 0. Characterization of isolated Kupffer cells includes assessing morphology in plated format and evaluating functional responses to LPS stimulation.

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Product Overview

Lonza cord blood CD34+ cells (2C-101X) contain hematopoietic stem and progenitor cells, are isolated from cord blood mononuclear cells (MNCs) via positive immunomagnetic separation. They are ideal for studies relating to transplantation and stem cells and humanized mouse model development. The number of cells typically obtained from cord blood can be limiting and pose a challenge. Cord blood derived CD34+ (2C-101X) consists of ≥2 million cells hematopoietic stem cells from the same donor. Cells are ethically sourced and are isolated using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. Large inventory supports stable supply from a single vendor.

Guarantee

Lonza guarantees the performance of Cells only if appropriate Lonza recommended Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Instructions

1  of 

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.