lonza 2C-101A,人脐带血 CD34+ 祖细胞,2M-101,1M-105

Catalog #: 2C-101A

Cryopreserved ampule of Human Cord Blood CD34+ containing ≥500,000 cells

Product Overview

脐带血(通常缩写为 HUCB)CD34+ 细胞已知含有造血干细胞和祖细胞,是与移植和干细胞相关的研究的理想选择,因为脐带血来源的细胞往往比其他组织来源的类似细胞更幼稚。

High Purity and Viability

Lonza 脐带血 CD34+ 细胞通过阳性免疫磁性分离从脐带血单核细胞 (MNC) 中分离出来,并保证具有 ≥90% 的纯度,如 CD34+ 表达细胞所示。 它们保证含有≥500,000 个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1 和乙型肝炎。 为购买的每个细胞批次提供分析证书。

由优化的媒体系统支持

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能(根据应用,可能需要额外的细胞因子)。 含有血清的替代培养基也是可以接受的。 有关含血清培养基的建议,请联系科学支持。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

Benefits

  • More naive than equivalent bone marrow-derived CD34+ cells
  • Wide range of donors available
  • High purity with > 90% CD34+ cells
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Applications

  • humanized mice
  • autoimmune disease research
  • immunology

Content & Storage

Content

1 x Cryopreserved ampule of Cord Blood CD34+ cells containing ≥500,000 cells

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Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 100,000 2M-101

人骨髓 CD34+ 祖细胞,冷冻保存,100,000 2M-101

Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells
Human Bone Marrow Stromal Cells, 5 Million 2M-302
Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells
Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

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Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells
hMSC – Human Mesenchymal Stem Cells PT-2501

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Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
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lonza R-ASM-580,lonza CC-5034,大鼠主动脉平滑肌细胞 (rAoSMC)

Catalog #: R-ASM-580
Cryopreserved ampule of Rat Aortic Smooth Muscle Cells containing ≥ 500,000 cells

Product Overview

 

冷冻保存的大鼠 AoSMC 从成年 Sprague Dawley 大鼠中分离。 这些细胞的特征是平滑肌肌动蛋白测试≥95% 阳性。

使用这些细胞进行血管生成、动脉粥样硬化和心血管研究。 细胞也可用于交叉比较您的研究与 CloneticsTM 原代人主动脉平滑肌细胞。

推荐介质: Lonza’s classical DMEM/F12

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Content & Storage

Content

≥ 500,000 cells in a 0.5 mL cell suspension

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ReagentPack™ Subculture Reagents, 100 mL CC-5034

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Gentamicin sulfate-Amphotericin (GA-1000) , 5 mL CC-4083

硫酸庆大霉素-两性霉素 (GA-1000),5 mL CC-4083

Rat Aortic Smooth Muscle Cells (rAoSMC)
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

Rat Aortic Smooth Muscle Cells (rAoSMC)
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
Rat Aortic Smooth Muscle Cells (rAoSMC)
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032
Rat Aortic Smooth Muscle Cells (rAoSMC)
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

Rat Aortic Smooth Muscle Cells (rAoSMC)
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960
Rat Aortic Smooth Muscle Cells (rAoSMC)
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1002

Rat Aortic Smooth Muscle Cells (rAoSMC)
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010
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lonza PT-5005,人内脏前脂肪细胞,P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

Catalog #: PT-5005
Preadipocytess, visceral, ≥1 million cells

Product Overview

PoieticsTM 正常人前脂肪细胞 (HprAD) 是从正常(非糖尿病)供体的皮下或内脏脂肪中分离出来的。来自糖尿病供体的前脂肪细胞可作为单独的产品获得。相关的额外捐赠者信息,包括体重指数 (BMI) 可通过联系科学支持获得。冷冻保存的 PoieticsTM 正常人前脂肪细胞 (HprAD) 保证每安瓿含有≥100 万或≥400 万(仅皮下)细胞,并且脂肪分化检测呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。推荐培养基:PGMTM -2 Preadipocyte Growth Medium-2 BulletKitTM 培养基(提供增殖培养基和分化培养基,保证诱导分化为成熟、功能活跃的脂肪细胞;每瓶 PGM-2TM BulletKitTM 培养基都含有足够的分化培养基以完全分化1000 万个前脂肪细胞 [10 个完整的 96 孔板] 变成成熟的、功能活跃的脂肪细胞)。 PGMTM-2 培养基以 BulletKitTM 培养基(目录号 PT-8002)的形式提供,其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。基础培养基(PGMTM-2 基础培养基;目录号 PT-8202)和必要的补充剂(PGMTM-2 SingleQuotTM 试剂盒;目录号 PT-9502)也可以单独购买。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

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P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

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Human Visceral Preadipocyte Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
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P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

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Human Visceral Preadipocyte Cells
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096
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P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

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lonza PT-3925,hMSC人间充质干细胞成软骨分化基础培养基

Catalog #: PT-3925
hMSC Chondrogenic Differentiation Basal Medium, 185 mL

Product Overview

干细胞具有分化成包括软骨细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 软骨分化 BulletKitTM 培养基是一种含有血清的培养基,旨在通过添加 TGF-β3 诱导人骨髓来源的间充质干细胞在短短 14 天内向成熟、功能活跃的软骨细胞分化。然后可以通过多种方法验证分化,包括用爱新蓝或番红染色。用 hMSC 软骨分化 BulletKitTM 培养基*:人骨髓来源的干细胞 人脐带血来源的干细胞 人脐带基质来源的干细胞 C57BL/6 小鼠骨髓来源的干细胞 人脂肪来源的干细胞 人腘绳肌腱衍生干细胞 hMSC 软骨分化 BulletKitTM 培养基,添加了 TGF-β3(单独出售),提供了一种分化培养基,可保证诱导人骨髓来源的间充质干细胞软骨分化为成熟的功能活跃的软骨细胞;每瓶 hMSC 软骨分化 BulletKitTM 培养基都含有足够的分化培养基,可以将至少 200 万个人骨髓来源的间充质干细胞 [大约 4 个完整的 24 孔板] 完全分化为成熟的功能活跃的软骨细胞。 hMSC 软骨分化培养基以 BulletKitTM 培养基(目录号 PT-3003)的形式提供,其中包括基础培养基和必要的补充剂(添加 TGF-β3 [单独出售,目录号 PT-4124]),用于人骨髓间充质干细胞的成软骨分化。基础培养基(Differentiation Basal Medium – Chondrogenic;目录号 PT-3925)和必要的补充剂(HMSC SQ 试剂盒 – 软骨;目录号 PT-4121 和用于 HMSC Chondro diff 的 TGF Beta 3;目录号 PT-4124)也可以单独购买。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助,请联系 Lonza Scientific Support。 *从客户数据生成的单元格列表。 Lonza 通常只将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用,因此只能保证这种培养基与这些细胞一起成功使用。

lonza 2M-101D,人骨髓 CD34+ 祖细胞,人骨髓基质细胞 2M-302

Catalog #: 2M-101D

Cryopreserved ampule of Bone Marrow CD34+ Progenitor Cells containing ≥2 million cells. Mononuclear cells are isolated using positive immunomagnetic selection.

Product Overview

骨髓 CD34+ 细胞含有造血干细胞和祖细胞,并且已知可分化成所有不同的血细胞类型。

您可以信赖的纯度和活力

Lonza骨髓CD34+细胞通过阳性免疫磁分离从骨髓单个核细胞(MNC)中分离出来,保证纯度≥90%。根据订购的产品,它们保证每个安瓿含有≥100,000、≥300,000、≥500,000、≥100 万或 ≥200 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、丙型肝炎和乙型肝炎。为购买的每个细胞批次提供分析证书。提供各种大小的新鲜骨髓 CD34+ 细胞,每个供体至少需要两瓶。有关新鲜细胞的尺寸和价格信息,请联系客户服务。

由优化的培养系统支持

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能(根据应用可能需要额外的细胞因子) 也可以接受含有血清的替代培养基。有关含血清培养基的建议,请联系科学支持。

细胞类型和组织来源的选择

复杂的研究通常需要灵活性。这就是为什么我们提供分离和纯化的骨髓 CD34+ 细胞以及未加工的骨髓,以防您需要分离自己的细胞。骨髓 CD34+ 细胞非常适用于需要来自单个供体的大量细胞的研究或调查不同年龄、性别或种族供体的细胞差异的研究。但是,如果您的研究需要更多幼稚细胞,脐带血 CD34+ 细胞可能是更好的选择。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

Benefits

  • Ready to use
  • Wide donor variety
  • High purity with > 90% CD34+ cells
  • Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34+ cells)

Content & Storage

Content

1 x Cryopreserved ampule of Human Bone Marrow CD34+ containing ≥2 million cells

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lonza 4W-350,原代细胞和干细胞,人外周血 CD3+ Pan T 细胞

Catalog #: 4W-350
Human CD3+ T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 25 million cells, cryopreserved

Product Overview

Leukapheresed Pan T 细胞 (CD3+) 是使用间接免疫磁性标记系统从单核细胞中阴性选择的。 细胞在冷冻管中的 Lonza Cryo 培养基中冷冻保存。 解冻后使用 FACS 测定确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。

由 AllCells® 制造

Applications

– CAR-T research

– Cell Therapy process development

– Immunology

– Immuno-oncology

Content & Storage

Content

1 vial containing ≥ 25 million cryopreserved cells

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lonza R-HI-501,大鼠脑海马神经元,PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Catalog #: R-HI-501
Cryopreserved ampule of Rat Brain Hippocampus Neurons containing ≥ 1 million cells

Product Overview

即用型 CloneticsTM 大鼠脑海马神经元来自大鼠 (E18, 19) 脑,是通过标准化方法制备的高质量原代胚胎脑神经元细胞(包括神经胶质细胞)的细胞悬液,可立即进行培养。

每瓶细胞含有大约 100 万个活细胞。 这将使用推荐的电镀密度和培养基播种到 24 孔板的 5 个或更多孔和 96 孔板的 25 个或更多孔中。 电镀后最初几天会发生细胞死亡,并观察到碎片。 这很正常。 培养约 4 天后,细胞将形成神经突网络,到第 7 天,碎片将最少。

推荐媒体:PNGMTM BulletKitTM (CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥1,000,000 cryopreserved cells per ampule

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P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
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P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

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Rat Brain Hippocampus Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
Rat Brain Hippocampus Neurons
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

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Rat Brain Hippocampus Neurons
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
Rat Brain Hippocampus Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

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lonza,原代细胞和干细胞,lonza L7™ hPSC Matrix

Catalog #: FP-5020

Recombinant, xeno free and defined L7TM hPSC Matrix, 1 mg for up to 1000 cm2

Product Overview

重组、无异源和明确的 hPSC 基质,以支持许多 hPSC 系的高效附着、保持多能性和稳定的核型。

Benefits

  • Recombinant, xeno-free and defined hPSC Matrix
  • Supports efficient hPSC attachment
  • Maintains pluripotency and stable karyotype

Content & Storage

Content

1 x L7TM hPSC Matrix, 1 mg for up to 1000 cm2

 

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lonza 3P-105B,新鲜人类 Leukopak,人外周血单个核细胞hPBMC-CC-2702

Catalog #: 3P-105B
从正常外周血中采集的新鲜人白细胞分离产品,含有≥50亿个细胞

Product Overview

Leukopak 是一种高度富集的白细胞分离衍生产品,从 IRB 批准的收集设施获得。与标准静脉穿刺采集方法或血沉棕黄层产品相比,Leukopaks 含有更高浓度的单核细胞、B 细胞、T 细胞、干细胞/祖细胞、树突细胞和其他细胞类型。

根据 IRB 批准的协议,使用 Spectra Optia® 单采系统与健康、同意的供体进行白细胞分离术。使用 ACD-A 作为抗凝剂,从 2-2.5 个供体血液体积中将富含 MNC 的细胞连续收集到无菌袋中。在白细胞分离后,大约 120 mL 的自体血浆被自动收集到细胞池中。收集后,使用 AO/PI 确定总有核细胞计数。将确定数量的 50 亿个 apheresed 细胞(包括目标溢出)转移到无菌容器中。提供的细胞的总体积和浓度会有所不同。基于此计数,该产品在释放时被填充以提供不少于 50 亿个细胞。

Leukopaks 通常含有高达 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞和 10% 的 NK 细胞、3% 的粒细胞和 3% 的血细胞比容。实际百分比会因捐赠者而异。

由 AllCells® 制造

新鲜的人源产品需支付额外的运费

Benefits

  • guaranteed minimum quantity of MNCs from a single donor
  • higher concentration of leukocytes compared to buffy coats
  • IRB-approved donor management and collection process

Content & Storage

Content

1 bag containing ≥ 5 billion fresh cells

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Fresh Human Leukopak (5 Billion Cells)
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702
人外周血单个核细胞 (hPBMC),冷冻保存,5000 万个 CC-2702

lonza PT-4503,ADSC – Apidose 衍生干细胞生长培养基 SingleQuots™ 补充剂和生长因子

Catalog #: PT-4503
ADSC Growth Medium SingleQuotsTM Kit

Product Overview

干细胞具有作为未分化细胞类型增殖的独特能力,然后在适当刺激时分化成多个细胞谱系。 Lonza 的 ADSC 人脂肪来源的干细胞生长 BulletKitTM 培养基是一种含有血清的培养基,旨在增殖处于未分化状态的人脂肪来源的间充质干细胞(正常和患病)。 Lonza 的 ADSC 人脂肪衍生干细胞生长 BulletKitTM 培养基中使用的血清经过专门筛选,以确保血清既不促进细胞自发分化,也不抑制细胞分化为成骨、软骨或脂肪谱系(当用适当的分化培养基适当刺激时) . ADSC 人脂肪源性干细胞生长 BulletKitTM 培养基以 BulletKitTM 培养基(目录号 PT-4505)的形式提供,其中包括基础培养基和人脂肪源性间充质干细胞增殖所需的补充剂。基础培养基(ADSC 基础培养基;目录号 PT-3273)和必要的补充剂(ADSC-GM SQ 试剂盒;目录号 PT-44503)也可以单独购买。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

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ADSC – Apidose-Derived Stem Cells Growth Medium SingleQuots™ Supplements and Growth Factors
hADSC Human Adipose-Derived Stem Cells PT-5006
hADSC 人体脂肪干细胞 PT-5006

lonza AC-2509,牛脑微血管内皮细胞,lonza CC-2565

Catalog #: AC-2509

Cryopreserved ampule of bMVEC-B, Bovine Brain Microvascular Endothelial Cells containing ≥ 750,000 cells.

 

Product Overview

牛脑微血管内皮细胞系统 (bMVEC-B) 是血脑屏障的模型。 该系统旨在显着提高研究人员研究药物通过血脑屏障的主动和被动转运、研究脑内皮细胞紧密连接以及研究脑微血管内皮细胞基础生物学的能力。

bMVEC 系统由来自多个供体大脑库的冷冻保存的原代牛脑微血管内皮细胞组成。 内皮细胞表达细胞紧密连接(ZO-1 和 Claudin-1 蛋白阳性)和血脑屏障中发现的转运特征(P-糖蛋白功能)。 因此,该系统可用于早期 ADME-Tox 筛选试验,以证明测试化合物是否可能穿过血脑屏障。

推荐培养基:EBM-2 基础培养基,500 mL (CC-3156)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models
  • Neurotransmitter Function
  • Signaling Pathways

 

Content & Storage

Content

Cryopreserved ampule with ≥ 750,000 cells/vial

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Bovine Brain Microvascular Endothelial Cells
RAFT™ 24-Well Small Transwell Kit, 3D Reagents and Transwell Absorbers for 24-Well Inserts – 12 Reactions 016-1R18

RAFT™ 24 孔小 Transwell 试剂盒、3D 试剂和用于 24 孔插入物的 Transwell 吸收器 – 12 种反应 016-1R18

Bovine Brain Microvascular Endothelial Cells
NHA – Human Astrocytes CC-2565
Bovine Brain Microvascular Endothelial Cells
RAFT™ 24-Well Small Kit, 3D Reagents & 24-Well Plate Absorbers – 12 Reactions 016-1R16
RAFT™ 24 孔小试剂盒、3D 试剂和 24 孔板吸收器 – 12 种反应 016-1R16

lonza CC-2504,原代细胞和干细胞,NHEM-Neo-人类表皮黑色素细胞新生儿

Catalog #: CC-2504
NHEM-Neo, Epidermal Melanocytes, Neonatal

Product Overview

成人和新生儿人类表皮黑色素细胞 (NHEM) 是位于所有哺乳动物皮肤表皮层的色素生成细胞。黑色素细胞的功能是产生黑色素,黑色素是赋予皮肤颜色的色素。黑色素保护皮肤免受紫外线伤害。皮肤由2个主要层组成,表皮和真皮。表皮由多个角质形成细胞层组成。黑素细胞位于表皮的基底层。星状或树突状黑素细胞形态允许黑素细胞细胞体驻留在表皮的基底层,而树突与表皮上层的角质形成细胞结合。这种关联允许黑色素细胞将黑色素从细胞体通过树突运输到角质形成细胞中,从而导致皮肤着色。

黑素细胞研究有两个主要领域——化妆品和医学。化妆品研究中心专注于改变皮肤颜色的能力。医学研究通常集中在黑色素瘤以及各种皮肤病或相关疾病,如白化病和白癜风、激素治疗、甲状腺疾病、牛皮癣、烧伤和疤痕。

我们冷冻保存的正常人表皮黑色素细胞通过 8 次群体倍增保证,表达 Mel-5 和 L-Dopa 转化为黑色素。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

注意:黑色素细胞对过度胰蛋白酶化极为敏感。使用 Lonza 的胰蛋白酶/EDTA 溶液 (CC-5012) 并在使用前用 Versene®(17-711E) 进行 1:1 稀释。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

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NHEM-Neo – Human Epidermal Melanocytes, Neonatal
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled 00192906

NHEK-Neo – 人表皮角质形成细胞,新生儿,汇集 00192906

NHEM-Neo – Human Epidermal Melanocytes, Neonatal
NHDF-Neo – Human Dermal Fibroblasts, Neonatal CC-2509
NHEM-Neo – Human Epidermal Melanocytes, Neonatal
NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor 00192907

NHEK-Neo – 人类表皮角质形成细胞 – 新生儿,单一供体 00192907

NHEM-Neo – Human Epidermal Melanocytes, Neonatal
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor CC-2505

HMVEC-dNeo – 人真皮微血管内皮细胞,新生儿,单供体 CC-2505

NHEM-Neo – Human Epidermal Melanocytes, Neonatal
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Pooled CC-2516
HMVEC-dNeo – 人真皮微血管内皮细胞,新生儿,混合 CC-2516

lonza CC-4092,原代细胞和干细胞,牛脑提取物 (BBE)

Catalog #: CC-4092
Bovine Brain Extract (3 mg/ml, 2 ml) contains endothelial cell proliferation growth factors

Product Overview

牛脑提取物的浓度为 3 mg/ml,单独出售或作为与内皮细胞培养基相关的 Lonza 的 SingleQuotsTM 试剂盒中的一个成分出售。

Content & Storage

Content

1 x Bottle containing 3 mg/mL BBE, 2 mL

Related Products
Bovine Brain Extract (BBE), 2 ml
EBM™ Endothelial Cell Growth Basal Medium, 500 mL CC-3121

EBM™ 内皮细胞生长基础培养基,500 mL CC-3121

Bovine Brain Extract (BBE), 2 ml
Bovine Brain Extract (BBE), 2 ml CC-4092
 牛脑提取物 (BBE),2 ml CC-4092

lonza 2W-400B,原代细胞和干细胞,人外周血 CD14+ 单核细胞

Catalog #: 2W-400B
人外周血 CD14+ 单核细胞冻存安瓿,阳性选择,含有≥2000 万个细胞

Product Overview

单核细胞存在于循环外周血中,占总单核细胞群的 10-20%。单核细胞的特征在于它们的 CD14 表型表达,因此,通常称为 CD14+ 单核细胞。它们作为循环单核细胞和分化成组织巨噬细胞在宿主防御中发挥重要作用,并且可以分化成具有强大抗原呈递能力的树突状细胞。

您可以信赖的细胞

Lonza Human Peripheral Blood CD14+ 单核细胞是从经过筛选的、健康的、未经治疗的(未动员的外周血)供体的外周血中分离出来的。简而言之,单核细胞 (MNC) 是从单采术衍生的外周血中分离出来的。然后通过针对 CD14 的阳性免疫磁性选择进一步纯化 MNC 群体。冷冻保存的 CD14+ 单核细胞保证表达 CD14,并且根据订购的产品,每个安瓿将包含 ≥10、20 或 4000 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

细胞类型和组织来源的选择

造血和免疫研究需要多种供体和细胞类型。这就是为什么 Lonza 提供来自各种供体的多种不同外周血细胞类型,包括 PBMC 和树突状细胞等。此外,如果您有兴趣探索不同成熟状态或来自不同组织的单核细胞之间的差异,我们提供骨髓单核细胞。

Supported by Lonza Media

The recommended medium varies by application (see below). Please contact Scientific Support for additional culturing recommendations. Media recommendations for macrophage and dendritic cell differentiation are recommendations only.

  • Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
  • Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
  • Dendritic Cells: LGM-3TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza CD14+ 单核细胞没有针对巨噬细胞或树突状细胞分化进行常规质量控制测试,并且细胞保修不保证分化。 产生分化可能需要其他因素,包括细胞外基质或培养条件。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 20 million cells

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Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC),冷冻保存,5000 万个 CC-2702

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒(25 次测试)LT07-218

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million

 Human CD14+ Monocytes

Human CD14+ monocytes differentiating to macrophages

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 20 million

lonza 00194911,D-HBE-As-患病的人支气管/气管上皮细胞-哮喘

Catalog #: 00194911
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells from donors having Asthma

Product Overview

Lonza 的哮喘气道细胞包括肺成纤维细胞、支气管上皮细胞和支气管平滑肌细胞。支气管上皮细胞从肺分叉上方的气道上皮衬里分离出来。

哮喘是一种气道炎症性疾病,可导致患者出现喘息、气短、胸闷和咳嗽。严重程度因个体患者而异,对药物治疗的反应也是如此。嗜酸性粒细胞与支气管上皮细胞的粘附以及气道平滑肌和上皮下成纤维细胞的相互作用在哮喘病理学中起关键作用。在哮喘发作期间,细支气管平滑肌组织可以收缩和减少气道中的空气流动。炎症或粘液分泌过多可进一步减少气流量。

来自哮喘供体的冷冻保存的患病支气管上皮细胞的支原体、细菌、酵母和真菌检测呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。我们的目录号 00194911S 适用于气液界面研究,并保证促进气道上皮的完全分化,通过形成具有良好屏障功能(跨上皮阻力)、分泌表型(粘蛋白分泌)和纤毛发生的极化上皮表达。分化通常会在培养的第 22 天发生。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Applications

  • Gene expression pathways
  • Phenotypic changes to compounds
  • Transfect diseased cells
  • Co-culture with immune cells
  • Airway remodeling studies
  • Cellular interactions

Content & Storage

Content

1 x Cryopreserved ampule of D-HBE-As containing ≥500,000 cells

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D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S

NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S

D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma 00194850

D-BSMC-As – 患病的人支气管平滑肌细胞 – 哮喘 00194850

D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170

BEGM™ 支气管上皮细胞生长培养基 BulletKit™ CC-3170

D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-3225,原代细胞和干细胞,CDM™ 软骨细胞分化培养基 BulletKit™

Catalog #: CC-3225
CDMTM Chondrocyte Differentiation BulletKitTM

Product Overview

  • CDMTM Chondrocyte Differentiation Medium is specially designed for the differentiation of chondrocytes. The BulletKitTM is conveniently packaged with basal medium and supplements included for the differentiation of chondrocytes.
  • CGMTM Chondrocyte Growth Medium is also available and sold separately for expansion of human chondrocytes

Content & Storage

Content

1 x Bottle Basal Medium 250 mL
1 x Yellow Cap Vial with R3-IGF 0.50 mL
1 x Blue Cap Vial with TGF-beta 1.25 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Red Cap Vial with GA-1000 0.25 mL
1 Bottle FBS 12.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL

Related Products
CDM™ Chondrocyte Differentiation Medium BulletKit™
NHAC-kn – Human Knee Articular Chondrocytes CC-2550
CDM™ Chondrocyte Differentiation Medium BulletKit™
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216

CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

CDM™ Chondrocyte Differentiation Medium BulletKit™
CDM™ Chondrocyte Differentiation Basal Medium CC-3226

CDM™ 软骨细胞分化基础培养基 CC-3226

CDM™ Chondrocyte Differentiation Medium BulletKit™
CDM™ Chondrocyte Differentiation Medium SingleQuots™ Supplements and Growth Factors CC-4408
CDM™ 软骨细胞分化培养基 SingleQuots™ 补充剂和生长因子 CC-4408

lonza CC-4194,OGM™ 成骨细胞生长培养基 BulletKit™ CC-3207,OGM™ 成骨细胞分化培养基 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4194
OGMTM Osteoblasts Differentiation SingleQuotsTM Kit

Product Overview

OGMTM 分化 SingleQuotTM 试剂盒是单独出售的组件,不包括基础培养基。 这些是 OGMTM 成骨细胞生长培养基 BulletKitTM(目录号 CC-3207)的一部分。 完整的 OGMTM BulletKitTM 由基础培养基和用于生长和分化的补充剂组成,可以使用目录号 CC-3207 一起购买。

CloneticsTM OGMTM 成骨细胞生长培养基专为人类成骨细胞的生长和扩增而配制。 该试剂盒含有 10% 的血清。 OGMTM 分化补充剂也包括在内,建议用于分化测定。 该试剂盒包装方便,包括含有生长和分化补充剂的基础培养基,用于成骨细胞的完全培养

Benefits

Applications:

  • In vitro bone models
  • Mineralization
  • Osteoporosis
  • Gene expression
  • Hormone treatments
  • Osteopenia
  • Paget’s Disease

Content & Storage

Content

1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Bottle B-Glycerophosphate 5.00 mL

Related Products
OGM™ Osteoblast Differentiation Medium SingleQuots™ Supplements and Growth Factors
NHOst – Human Osteoblasts CC-2538

NHOst – 人类成骨细胞 CC-2538

OGM™ Osteoblast Differentiation Medium SingleQuots™ Supplements and Growth Factors
OGM™ Osteoblast Growth Medium BulletKit™ CC-3207
 OGM™ 成骨细胞生长培养基 BulletKit™ CC-3207

lonza CC-2579,原代细胞和干细胞,USMC-人脐动脉平滑肌细胞

Catalog #: CC-2579
UASMC, Umbilical Artery Smooth Muscle Cells

Product Overview

人血管平滑肌细胞来自多种组织类型,包括主动脉 (AoSMC)、冠状动脉 (CASMC)、肺动脉 (PASMC) 和脐动脉 (UASMC)。 冷冻保存的细胞在第 3 代运输。

CloneticsTM 人主动脉平滑肌细胞保证 15 次群体倍增,分化后 α 平滑肌肌动蛋白染色呈阳性,von Willebrand 因子 VIII 染色呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Applications

  • Stroke
  • Diabetes
  • Vascular research
  • Artherosclerosis
  • Hypertension

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
UASMC – Human Umbilical Artery Smooth Muscle Cells
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2 C2517A

HUVEC – EGM™-2 C2517A 中的人脐静脉内皮细胞,单一供体

UASMC – Human Umbilical Artery Smooth Muscle Cells
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517

HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞,单一供体

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960

UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002
UASMC – Human Umbilical Artery Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

lonza PT-8202,原代细胞和干细胞,PBM-2 前脂肪细胞基础培养基-2

Catalog #: PT-8202
PGMTM-2 Basal Medium, 500 mL

Product Overview

PGMTM -2 前脂肪细胞生长培养基-2 BulletKitTM 培养基提供增殖培养基和分化培养基,保证诱导分化成成熟的、功能活跃的脂肪细胞; 每瓶 PGM-2TM BulletKitTM 培养基都含有足够的分化培养基,可将 1000 万个前脂肪细胞 [10 个完整的 96 孔板] 完全分化为成熟的功能活跃的脂肪细胞。 PGMTM-2 培养基以 BulletKitTM 培养基(目录号 PT-8002)的形式提供,其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。 基础培养基(PGMTM-2 基础培养基;目录号 PT-8202)和必要的补充剂(PGMTM-2 SingleQuotTM 试剂盒;目录号 PT-9502)也可以单独购买。

lonza PT-3273,ADSC 基础培养基,hADSC 人体脂肪干细胞 PT-5006

Catalog #: PT-3273
Basal Medium for Adipose Derived Stem Cells

Product Overview

干细胞具有作为未分化细胞类型增殖的独特能力,然后在适当刺激时分化成多个细胞谱系。 Lonza 的 ADSC 人脂肪来源的干细胞生长 BulletKitTM 培养基是一种含有血清的培养基,旨在增殖处于未分化状态的人脂肪来源的间充质干细胞(正常和患病)。 Lonza 的 ADSC 人脂肪衍生干细胞生长 BulletKitTM 培养基中使用的血清经过专门筛选,以确保血清既不促进细胞自发分化,也不抑制细胞分化为成骨、软骨或脂肪谱系(当用适当的分化培养基适当刺激时) .

ADSC 人脂肪源性干细胞生长 BulletKitTM 培养基以 BulletKitTM 培养基(目录号 PT-4505)的形式提供,其中包括基础培养基和人脂肪源性间充质干细胞增殖所需的补充剂。基础培养基(ADSC 基础培养基;目录号 PT-3273)和必要的补充剂(ADSC-GM SQ 试剂盒;目录号 PT-44503)也可以单独购买。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

Content & Storage

Content

  •  ADSC Basal Medium (1 bottle)
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lonza FP-5013,原代细胞和干细胞,lonza L7™ hPSC 传代解决方案

Catalog #: FP-5013
L7TM hPSC Passaging Solution, 100 mL

Product Overview

Non-enzymatic cell detachment formulation that gently dislodges multicellular colony fragments without the need for mechanical manipulation.  This user friendly method simplifies routine maintenance of your valuable hESC and iPSC lines.

无需机械操作即可温和去除多细胞集落碎片的非酶细胞分离配方。 这种用户友好的方法简化了您宝贵的 hESC 和 iPSC 生产线的日常维护。

lonza CC-4510,原代细胞和干细胞,内皮素 3 生长补充剂 (ET-3)

Catalog #: CC-4510
Endothelin-3 (ET-3), 130 µg

Product Overview

ET-3 生长补充剂是成人黑色素细胞生长所需的额外生长因子。 它单独出售以补充 MGMTM-4 BulletKitTM(目录号 CC-3249),以获得专门为生长成人黑色素细胞而设计的完整配方。 除了限制培养中的其他细胞类型污染外,ET-3 还提供增强的生长和铺板优势。

Content & Storage

Content

1 x Lyophilized Vial Containing 130 ug

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lonza PT-4121,hMSC 人间充质干细胞成软骨分化培养基 SingleQuotsTM 添加剂和生长因子

Catalog #: PT-4121
hMSC Chondrogenic Differentiation SingleQuotsTM Kit

Product Overview

干细胞具有分化成包括软骨细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 软骨分化 BulletKitTM 培养基是一种含有血清的培养基,旨在通过添加 TGF-β3 诱导人骨髓来源的间充质干细胞在短短 14 天内向成熟、功能活跃的软骨细胞分化。然后可以通过多种方法验证分化,包括用爱新蓝或番红染色。用 hMSC 软骨分化 BulletKitTM 培养基*:人骨髓来源的干细胞 人脐带血来源的干细胞 人脐带基质来源的干细胞 C57BL/6 小鼠骨髓来源的干细胞 人脂肪来源的干细胞 人腘绳肌腱衍生干细胞 hMSC 软骨分化 BulletKitTM 培养基,添加了 TGF-β3(单独出售),提供了一种分化培养基,可保证诱导人骨髓来源的间充质干细胞软骨分化为成熟的功能活跃的软骨细胞;每瓶 hMSC 软骨分化 BulletKitTM 培养基都含有足够的分化培养基,可以将至少 200 万个人骨髓来源的间充质干细胞 [大约 4 个完整的 24 孔板] 完全分化为成熟的功能活跃的软骨细胞。 hMSC 软骨分化培养基以 BulletKitTM 培养基(目录号 PT-3003)的形式提供,其中包括基础培养基和必要的补充剂(添加 TGF-β3 [单独出售,目录号 PT-4124]),用于人骨髓间充质干细胞的成软骨分化。基础培养基(Differentiation Basal Medium – Chondrogenic;目录号 PT-3925)和必要的补充剂(HMSC SQ 试剂盒 – 软骨;目录号 PT-4121 和用于 HMSC Chondro diff 的 TGF Beta 3;目录号 PT-4124)也可以单独购买。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助,请联系 Lonza Scientific Support。 *从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用,因此只能保证这种培养基与这些细胞一起成功使用

lonza M-ASM-330,原代细胞和干细胞,小鼠 C57 脑混合星形胶质细胞

Catalog #: M-ASM-330

Cryopreserved ampule of Mouse C57 Mixed Astrocytes containing ≥ 1 million cells

Product Overview

即用型 CloneticsTM 小鼠脑混合星形胶质细胞 (M-ASM-330) 从 C57 黑小鼠脑中获得,传代一次并制备成细胞悬浮液以供运输。

每个小瓶包含大约 100 万个细胞。 星形胶质细胞可以很容易地解冻和培养。 星形胶质细胞可以收获和重新铺板。 细胞在干冰上运输过夜。

推荐介质:AGMTM BulletKitTM (CC-3186)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥ 1,000,000 cryopreserved cells per ampule

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AGM™ Astrocyte Growth Medium BulletKit™ CC-3186

AGM™ 星形胶质细胞生长培养基 BulletKit™ CC-3186

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Mouse Neuron Nucleofector™ Kit VPG-1001

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Mouse Neuron Nucleofector™ Kit VAPG-1001
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大鼠脑 Cx-Hi-Cp 混合星形胶质细胞 R-ASM-530

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P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
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P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

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P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

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P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Mouse C57 Brain Mixed Astrocytes
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-4131,KGM™ 角质形成细胞生长培养基 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4131

Contains supplements and growth factors required for culturing Human Keratinocytes (basal media not included).

Product Overview

KGMTM SingleQuotTM 试剂盒是单独出售的组件,不包括基础培养基。 这些是 KGMTM 角质形成细胞生长培养基 BulletKitTM(目录号 CC-3111)的一部分,它由包装在一起的基础培养基和补充剂组成。

lonza,lonza 2M-101,LT07-218,PT-2501,原代细胞和干细胞,人骨髓 CD34+ 祖细胞,100,000

Catalog #: 2M-101

Cryopreserved ampule of Human Bone Marrow CD34+ containing ≥100,000 cells. Mononuclear cells are isolated using positive immunomagnetic selection.

冷冻保存的人骨髓 CD34+ 安瓿,含有 ≥100,000 个细胞。 使用阳性免疫磁性选择分离单核细胞。

Product Overview

Bone marrow CD34+ cells contain hematopoietic stem and progenitor cells and are known to differentiate into all the various blood cell types.

Purity and Viability You Can Count On

Lonza bone marrow CD34+ cells are isolated from bone marrow mononuclear cells (MNC) by positive immunomagnetic separation and are guaranteed to have a purity of ≥90%. They are guaranteed to contain either ≥100,000, ≥300,000, ≥500,000, ≥1 million, or ≥2 million viable cells per ampoule depending on the product ordered. HIV-1, hepatitis C, and hepatitis B are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Fresh bone marrow CD34+ cells are offered in various packaging sizes and require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.

Lonza骨髓CD34+细胞通过阳性免疫磁分离从骨髓单个核细胞(MNC)中分离出来,保证纯度≥90%。 根据订购的产品,它们保证每个安瓿含有≥100,000、≥300,000、≥500,000、≥100 万或 ≥200 万个活细胞。 并非所有供体和/或细胞批次都检测到 HIV-1、丙型肝炎和乙型肝炎。 为购买的每个细胞批次提供分析证书。 新鲜骨髓 CD34+ 细胞有多种包装尺寸,每个供体至少需要订购两瓶。 有关新鲜细胞的尺寸和价格信息,请联系客户服务。

Supported by an Optimized Culture System

HPGMTM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application) Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能(根据应用可能需要额外的细胞因子) 也可以接受含有血清的替代培养基。 有关含血清培养基的建议,请联系科学支持。

Choices in Cell Type and Tissue Source

Complex research often calls for flexibility. That’s why we offer isolated and purified bone marrow CD34+ cells as well as unprocessed bone marrow in case you need to isolate your own cells. Bone marrow CD34+ cells are ideal for studies requiring a large number of cells from a single donor or studies investigating differences in cells of donors of various ages, genders, or ethnicities. If however, your studies demand more naïve cells, cord blood CD34+ cells may be the better choice.

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

复杂的研究通常需要灵活性。 这就是为什么我们提供分离和纯化的骨髓 CD34+ 细胞以及未加工的骨髓,以防您需要分离自己的细胞。 骨髓 CD34+ 细胞非常适用于需要来自单个供体的大量细胞的研究或调查不同年龄、性别或种族供体的细胞差异的研究。 但是,如果您的研究需要更多幼稚细胞,脐带血 CD34+ 细胞可能是更好的选择。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助,请联系 Lonza Scientific Support。

Benefits

  • Ready to use
  • High purity with > 90% CD34+ cells
  • Wide donor variety
  • Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34+/- cells)

Content & Storage

Content

1 x Cryopreserved ampule Human Bone Marrow CD34+ containing ≥100,000 cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English
SDS – Danish

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

 

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Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞,500 万个 2M-302

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hMSC – Human Mesenchymal Stem Cells PT-2501

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MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
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lonza,lonza CC-3233,软骨细胞 ReagentPack™ 继代培养试剂

货号: CC-3233
Chondrocyte ReagentPackTM Subculture Reagent
软骨细胞 ReagentPackTM 继代培养试剂

Product Overview

Chondrocyte ReagentPackTM Subculture Kits have been designed specifically for the passaging of primary chondrocytes.

Each kit contains 100 ml each of:

  • Trypsin/EDTA
  • Trypsin Neutralizing Solution
  • HEPES Buffered Saline

The Chondrocyte ReagentPackTM Subculture Kit is recommended for use with Lonza’s primary chondrocytes for effective trypsinization and cell passaging process.

软骨细胞 ReagentPackTM 传代培养试剂盒专为原代软骨细胞的传代而设计。

每个套件包含 100 毫升:

胰蛋白酶/EDTA
胰蛋白酶中和液
HEPES 缓冲盐水
建议将 Chondrocyte ReagentPackTM Subculture Kit 与 Lonza 的原代软骨细胞一起使用,以实现有效的胰蛋白酶消化和细胞传代过程。

Content & Storage

Content

1 x Bottle Trypsin/EDTA 100 mL
1 x Bottle Trypsin Neutralizing Solution 100 mL
1x Bottle HEPES Buffered Saline Solution 100 mL

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lonza 3W-270,原代细胞和干细胞,lonza 新鲜人外周血单个核细胞 (MNcs)

Catalog #: 3W-270
Mononuclear cells from human peripheral blood, fresh, 100 million cells

Product Overview

使用 Spectra Optia™ 单采机收集单核细胞 (MNC)。 白细胞被耗尽RBC并洗涤以去除血小板。 我们典型的 MNC 产品包含大约 50% 的 T 细胞、20 个单核细胞、10 个 B 细胞、10% 的 NK 细胞,并且可能含有高达 5% 的粒细胞。 按照 IRB 协议从健康供体的离心管中收集细胞。 由 AllCells® 制造 新鲜的人源产品需支付额外运费

Content & Storage

Content

1 vial containing ≥ 20 million fresh cells

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lonza HUCS20P,原代细胞和干细胞,lonza DonorPlexTM 混合供体人肝细胞

Catalog #: HUCS20P
具有多种 CYP450、UGT、SULT 和转运蛋白活性特征的混合供体人肝细胞

Product Overview

对于 DMPK 研究而言,一致且可靠的汇集供体人肝细胞来源非常重要。 通过我们新的专有算法,我们能够预测 DonorPlexTM 人肝细胞的产量、活力和功能表型,这反过来将支持您的 DMPK 中更一致的结果。 每批汇集的 20 位供体、混合性别的人类肝细胞的特征在于活产率,以及 CYP1A2、CYP2A6、CYP2B6、CYP2C9、CYP2C19、CYP2E1、CYP3A4、UGT、SULT、OAT1B1/3、OCT 和 NTCP 活性。

凭借我们大量的单供体人类肝细胞库存,并使用我们的专有算法来预测表型,我们还可以定制生产具有表型的混合供体肝细胞批次,以满足您的需求。

DonorPlex™ 肝细胞 – 悬浮合格肝细胞非常适合短期(<4 小时)体外药物清除试验。 悬浮合格的肝细胞不适用于需要在粘附的单层中培养肝细胞的应用。

Benefits

  • Targeted pooling method for predictable metabolism
  • Precharacterized for 9 CYPs and 4 drug transporters
  • High viability and yields
  • Lot sizes < 2000 are possible

Applications

  • Suspension metabolism studies

Content & Storage

Content

1 x Cryopreserved ampule of Pooled, Suspension-Qualified Hepatocytes containing >5 million viable cells

Related Products
DonorPlexTM Pooled Donor Human Hepatocytes, 20 Donor, Mixed Gender
Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞,冷冻保存,可电镀 HUCPG

DonorPlexTM Pooled Donor Human Hepatocytes, 20 Donor, Mixed Gender
Human Hepatocytes, Cryopreserved Single Donor , Suspension HUCSD
人肝细胞,冷冻保存的单一供体,悬浮 HUCSD

lonza CC-3153,MEBM™ 乳腺上皮细胞生长基础培养基,HMEC人乳腺上皮细胞 CC-2551

Catalog #: CC-3153

MEBMTM Mammary Epithelial Basal Medium, Phenol Red Free, for culture of human mammary epithelial cells, 500 mL.

Product Overview

MEBMTM 是一种不含酚红的乳腺上皮细胞基础培养基,单独出售。 这可以与 1 套 CC-4136(乳腺上皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子)一起使用。

乳腺上皮细胞生长培养基已针对乳腺上皮细胞在无血清环境中的生长进行了优化。 该媒体系统已被 130 多种主要关注乳腺癌研究的独特出版物引用。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products
MEBM™ Mammary Epithelial Cell Growth Basal Medium, Phenol Red Free
MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ CC-3150

MEGM™ 乳腺上皮细胞生长培养基 BulletKit™ CC-3150

MEBM™ Mammary Epithelial Cell Growth Basal Medium, Phenol Red Free
HMEC – Human Mammary Epithelial Cells CC-2551
HMEC – 人乳腺上皮细胞 CC-2551

lonza 2S-101D,原代细胞和干细胞,人骨髓,未加工的人骨髓10 mL 1M-105

Catalog #: 2S-101D

含有≥500万个细胞的人骨髓单个核细胞的冷冻保存安瓿

Product Overview

骨髓单核细胞 (BMNC) 是单核细胞的混合群体,包括单核细胞、淋巴细胞以及造血干细胞和祖细胞。

通过密度梯度分离从全骨髓中分离冷冻保存的龙沙骨髓单核细胞。分离单核细胞消除红细胞和粒细胞,产生更稳定的细胞产物。

Lonza BMNCs 可从大量多样的供体库中获得新鲜*或冷冻。根据购买的产品,它们保证每个安瓿含有≥5、25、100、200 和 3 亿个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

细胞类型和组织来源的选择

Lonza 提供细胞类型和组织来源的选择,因此您可以选择适合您的研究和预算需求的产品。根据您选择的最终细胞,您可以选择未加工的骨髓、骨髓 MNC、骨髓 CD34+ 细胞、骨髓基质细胞(用作饲养细胞)或骨髓间充质干细胞。此外,如果骨髓不是您首选的组织来源,我们还提供脐带血来源的细胞。

Recommended Media and Reagents

  • HPGMTM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application). Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。 对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。 如果您在选择合适的培养基、试剂或方案方面需要帮助,请联系 Lonza Scientific Support。

*新鲜细胞悬浮在含有 5 mM EDTA 和 0.5% BSA 的 HBSS 中,每瓶包装尺寸有多种细胞可供选择。 有关新鲜细胞的尺寸和价格信息,请联系客户服务。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule BMNCs containing ≥5 million cells

Related Products

Human Bone Marrow MNCs, Cryopreserved, 5 Million
Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

未加工的人骨髓,新鲜,10 mL 1M-105

Human Bone Marrow MNCs, Cryopreserved, 5 Million
Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC),冷冻保存,5000 万个 CC-2702

Human Bone Marrow MNCs, Cryopreserved, 5 Million
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒(25 次测试)LT07-218

lonza R-G-535,大鼠小胶质细胞,大鼠脑 Cx-Hi-Cp 混合星形胶质细胞 R-ASM-530

Catalog #: R-G-535

Cryopreserved ampule of Rat Microglia containing ≥ 2 million cells

Product Overview

CloneticsTM 大鼠小胶质细胞是通过标准化方法制备的冷冻保存、即用型高品质细胞,可立即在补充的 DMEM 中培养。 每个小瓶在 0.25 ml 悬浮液中含有大约 200 万个细胞。

小胶质细胞是中枢神经系统 (CNS) 中的一种胶质细胞类型。 它们是神经胶质细胞网络的重要组成部分。 当大脑发育过程中发生程序性细胞死亡或中枢神经系统受伤时,小胶质细胞可以充当脑巨噬细胞。

推荐培养基:DMEM (Lonza No.12-604F) 补充有热灭活 FBS、青霉素/链霉素 (Lonza No. 17-602E) 和 D-葡萄糖 (Sigma No. G-8769)。

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥2,000,000 cryopreserved cells per ampule

Related Products
Rat Microglia
Rat Brain Cortex Astrocytes R-CXAS-520

大鼠脑皮质星形胶质细胞 R-CXAS-520

Rat Microglia
Rat Brain Striatum Astrocytes R-CPAS-522

大鼠脑纹状体星形胶质细胞 R-CPAS-522

Rat Microglia
Rat Brain Hippocampus Astrocytes R-HIAS-521

大鼠脑海马星形胶质细胞 R-HIAS-521

Rat Microglia
Rat Brain Cx-Hi-Cp Mixed Astrocytes R-ASM-530

大鼠脑 Cx-Hi-Cp 混合星形胶质细胞 R-ASM-530

Rat Microglia
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461
PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

lonza CC-4435,MGM™- 4 黑色素细胞生长培养基-4 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4435
MGMTM-4 Melanocyte SingleQuotsTM Kit

Product Overview

MGMTM -4 SingleQuotTM Kit are components sold separately and does not include the basal medium. These are part of the MGMTM Melanocyte Cell Growth Medium BulletKitTM (Catalog Number CC-3249). The full MGMTM BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number CC-3249.

The MGMTM-4 Melanocyte Growth Medium-4 BulletKitTM  is recommended media for melanocyte optimal growth and survival in culture. This medium is offered in our traditional BulletKitTM format, providing flexibility to add or omit components as research needs dictate.

ET-3 Growth Supplement, an additional growth factor required for adult melanocyte growth, is sold separately to supplement the MGMTM-4 BulletKitTM for a complete formulation specifically designed for growing adult melanocytes. ET-3 offers enhanced growth and plating advantages in addition to limiting other cell type contamination in culture.

Content & Storage

Content

1 x Natural Cap Vial with CaCl2 1.00 mL
1 x Amber Vial with PMA 0.50 mL
1 x Gray Cap Vial with hFGF-B 1.00 mL
1 x Lilac Cap Vial with Insulin 1.00 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Bottle FBS 2.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL

Related Products
MGM™- 4 Melanocyte Growth Medium-4 SingleQuots™ Supplements and Growth Factors
NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504

NHEM-Neo – 人表皮黑色素细胞,新生儿 CC-2504

MGM™- 4 Melanocyte Growth Medium-4 SingleQuots™ Supplements and Growth Factors
NHEM-Ad – Human Epidermal Melanocytes, Adult CC-2586

NHEM-Ad – 人表皮黑色素细胞,成人 CC-2586

MGM™- 4 Melanocyte Growth Medium-4 SingleQuots™ Supplements and Growth Factors
MGM™-4 Melanocyte Growth Medium-4 BulletKit™ CC-3249
MGM™-4 黑色素细胞生长培养基-4 BulletKit™ CC-3249

lonza HUCS50P,DonorPlex™ 混合供体人肝细胞,DonorPlexTM 混合供体人肝细胞男性 HUCS20PM

Catalog #: HUCS50P
汇集 50 个供体、混合性别的人类肝细胞,具有多种 CYP450、UGT、SULT 和转运蛋白活性。 药物代谢物鉴定、表征和周转动力学的理想选择。

Product Overview

对于 DMPK 研究而言,一致且可靠的汇集供体人肝细胞来源非常重要。通过我们新的专有算法,我们能够预测代表普通人群的产量、生存能力和功能表型。 DonorPlexTM 混合供体人类肝细胞,50 个供体,混合性别,是一个大约 50:50(最大 70:30)的混合性别,50 个供体池,其功能类似于普通人类肝脏。每个批次的特征在于可行产量,以及 CYP1A2、CYP2A6、CYP2B6、CYP2C9、CYP2C19、CYP2E1、CYP3A4、UGT、SULT、OAT1B1/3、OCT 和 NTCP 活性。

凭借我们大量的单供体人类肝细胞库存,并使用我们的专有算法来预测表型,我们还可以定制生产具有表型的混合供体肝细胞批次,以满足您的需求。

DonorPlexTM 混合供体人肝细胞,50 个供体,混合性别肝细胞非常适合在混合性别池中进行短期(<4 小时)体外药物清除试验。这些悬浮合格的肝细胞不适合需要在粘附单层中培养的肝细胞的应用。

Benefits

  • Targeted pooling method for predictable metabolism
  • Precharacterized for 9 CYPs and 4 drug transporters
  • High viability and yields
  • Lot sizes < 2000 are possible
Related Products
DonorPlex™ Pooled Donor Human Hepatocytes, 50 Donor, Mixed Gender
DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donor, Male HUCS10PM

DonorPlexTM 混合供体人肝细胞,10 个供体,男性 HUCS10PM

DonorPlex™ Pooled Donor Human Hepatocytes, 50 Donor, Mixed Gender
DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donor, Female HUCS10PF

DonorPlexTM 混合供体人肝细胞,10 个供体,女性 HUCS10PF

DonorPlex™ Pooled Donor Human Hepatocytes, 50 Donor, Mixed Gender
DonorPlexTM Pooled Donor Human Hepatocytes, 20 Donor, Male HUCS20PM

DonorPlexTM 混合供体人肝细胞,20 个供体,男性 HUCS20PM

DonorPlex™ Pooled Donor Human Hepatocytes, 50 Donor, Mixed Gender
DonorPlexTM Pooled Donor Human Hepatocytes, 20 Donor, Female HUCS20PF
DonorPlexTM 混合供体人肝细胞,20 个供体,女性 HUCS20PF

lonza小鼠脑 CD1 海马神经元 M-HI-401,大鼠脑海马神经元 R-HI-501

Catalog #: M-HI-401

Cryopreserved ampule of Mouse Brain CD1 Hippocampus Neurons containing ≥ 1,000,000 cells

含有 ≥ 1,000,000 个细胞的小鼠脑 CD1 海马神经元冻存安瓿

Product Overview

CloneticsTM 小鼠海马神经元分离自 E16-17、CD-1 小鼠。 海马体是人类和其他哺乳动物大脑的主要组成部分。 它属于边缘系统,在长期记忆和空间导航中起着重要作用。

CloneticsTM 小鼠海马神经元在初次传代时冷冻保存。 它们对 PGP 和 Tuj-1 染色呈阳性,对不育和支原体检测呈阴性。 使用 CloneticsTM PNGMTM 培养基时,细胞得到保证,每瓶含有 ≥1M 细胞。

推荐介质:PNGMTM BulletKitTM (CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥ 1,000,000 cryopreserved cells per ampule

Related Products
Mouse Brain CD1 Hippocampus Neurons
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Mouse Brain CD1 Hippocampus Neurons
Rat Brain Hippocampus Neurons R-HI-501

大鼠脑海马神经元 R-HI-501

Mouse Brain CD1 Hippocampus Neurons
Mouse Neuron Nucleofector™ Kit VAPG-1001

小鼠神经元 Nucleofector™ 试剂盒 VAPG-1001

Mouse Brain CD1 Hippocampus Neurons
Mouse Neuron Nucleofector™ Kit VPG-1001

小鼠神经元 Nucleofector™ 试剂盒 VPG-1001

Mouse Brain CD1 Hippocampus Neurons
Mouse Neuron Nucleofector™ Kit VVPG-1001

小鼠神经元 Nucleofector™ 试剂盒 VVPG-1001

Mouse Brain CD1 Hippocampus Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
Mouse Brain CD1 Hippocampus Neurons
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

Mouse Brain CD1 Hippocampus Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

Mouse Brain CD1 Hippocampus Neurons
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
Mouse Brain CD1 Hippocampus Neurons
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
Mouse Brain CD1 Hippocampus Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Mouse Brain CD1 Hippocampus Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-3250,lonza CC-3249,MBMTM-4 黑色素细胞生长基础培养基-4

Catalog #: CC-3250
MBMTM-4 Melanocyte Basal Medium

Product Overview

MBMTM -4 是一种黑色素细胞生长基础培养基,单独出售。 这可以与 1 套 CC-4435(黑色素细胞生长培养基 SingleQuotsTM 补充剂和生长因子)一起使用。 由基础培养基和补充剂组成的完整 MGMTM -4 BulletKitTM 可以使用目录号 CC-3249 一起购买。

MGMTM-4 黑色素细胞生长培养基-4 BulletKitTM 是推荐用于黑色素细胞在培养中最佳生长和存活的培养基。 该介质以我们传统的 BulletKitTM 格式提供,可根据研究需要灵活地添加或省略组件。

ET-3 生长补充剂是成人黑色素细胞生长所需的额外生长因子,单独出售,以补充 MGMTM-4 BulletKitTM 的完整配方,专门设计用于生长成人黑色素细胞。 除了限制培养中的其他细胞类型污染外,ET-3 还提供增强的生长和铺板优势。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products
MBMTM-4 Melanocyte Growth Basal Medium-4
NHEM-Ad – Human Epidermal Melanocytes, Adult CC-2586

NHEM-Ad – 人表皮黑色素细胞,成人 CC-2586

MBMTM-4 Melanocyte Growth Basal Medium-4
MGM™-4 Melanocyte Growth Medium-4 BulletKit™ CC-3249

MGM™-4 黑色素细胞生长培养基-4 BulletKit™ CC-3249

MBMTM-4 Melanocyte Growth Basal Medium-4
NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504
NHEM-Neo – 人表皮黑色素细胞,新生儿 CC-2504

lonza 00195275,D-HBE-COPD患病的人支气管/气管上皮细胞COPD,lonza V4SP-3096

Catalog #: 00195275
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells from donors having COPD

Product Overview

COPD 气道细胞包括肺成纤维细胞、支气管上皮细胞和支气管平滑肌细胞。 来自 COPD 供体的支气管上皮细胞是从肺分叉上方的气道上皮衬里分离出来的。

来自 COPD 供体的冷冻保存的患病人支气管上皮细胞的支原体、细菌、酵母和真菌检测呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。

为购买的每个细胞批次提供分析证书。 我们的目录号 00195275S 适用于气液界面研究,并保证促进气道上皮的完全分化,通过形成具有良好屏障功能(跨上皮阻力)、分泌表型(粘蛋白分泌)和纤毛发生的极化上皮表达。 分化通常会在培养的第 22 天发生。

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

Applications

  • Gene expression pathways
  • Phenotypic changes to compounds
  • Transfect diseased cells
  • Co-culture with immune cells
  • Airway remodeling studies
  • Cellular interactions

Content & Storage

Content

1 x Cryopreserved ampule containing ≥500,000 cells

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D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911

D-HBE-As – 患病的人支气管/气管上皮细胞 – 哮喘 00194911

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis 00196979

D-HBE-CF – 患病的人支气管/气管上皮细胞 – 囊性纤维化 00196979

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170

BEGM™ 支气管上皮细胞生长培养基 BulletKit™ CC-3170

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002
D-HBE-COPD – Diseased Human Bronchial/Tracheal Epithelial Cells – COPD
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza M-CX-300,原代细胞和干细胞,小鼠 C57 脑皮层神经元

Catalog #: M-CX-300
冷冻保存的小鼠 C57 皮层神经元安瓿,含有≥400 万个细胞

Product Overview

即用型 CloneticsTM 小鼠脑 C57 皮层神经元 (M-CX-300) 是从小鼠 C57 黑脑 (E14, 15) 中分离出来的。 这些以标准化方法制备的高质量原代胚胎脑神经元细胞(包括神经胶质细胞)的细胞悬液形式出售,并可立即培养。

每瓶皮层细胞含有大约 400 万个活细胞。 这将使用推荐的电镀密度和培养基播种到 24 孔板的 10 个或更多孔和 96 孔板的 50 个或更多孔中。 培养约 4 天后,细胞将形成神经突网络,到第 7 天,碎片将最少。

推荐介质:PNGMTM BulletKitTM (CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥4,000,000 cryopreserved cells per ampule

Related Products
Mouse C57 Brain Cortex Neurons
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Mouse C57 Brain Cortex Neurons
Rat Brain Cortex Neurons R-CX-500

大鼠大脑皮层神经元 R-CX-500

Mouse C57 Brain Cortex Neurons
Mouse CD1 Brain Cortex Neurons M-CX-400

小鼠 CD1 大脑皮层神经元 M-CX-400

Mouse C57 Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012

Mouse C57 Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
Mouse C57 Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

Mouse C57 Brain Cortex Neurons
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
Mouse C57 Brain Cortex Neurons
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
Mouse C57 Brain Cortex Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002
Mouse C57 Brain Cortex Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza 4Y-101C,原代细胞和干细胞,来自动员外周血的人 CD34+ 祖细胞

Catalog #: 4Y-101C
来自动员外周血的人 CD34+ 祖细胞的冷冻保存安瓿,使用阳性免疫磁性选择,包含 ≥ 100 万个细胞

Product Overview

使用免疫磁性 CD34 微珠标记系统积极选择动员的外周血 CD34+ 造血干细胞和祖细胞。 细胞在冷冻管中的 Lonza Cryo 培养基中冷冻保存。 解冻后使用 FACS 测定确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞

由 AllCells® 制造

Benefits

  • Obtain large quantities of CD34+ from a donor
  • Certificate of analysis (CoA) provided for each lot purchased
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

Applications

  • CAR-T therapy development
  • iPSC generation
  • Autoimmune disease
  • Immunology
  • Cancer research

Content & Storage

Content

1 x Cryopreserved ampule of Human CD34+ Progenitor Cells from Mobilized Peripheral Blood containing ≥ 1 million cells

Related Products
Human CD34+ Progenitor Cells from Mobilized Peripheral Blood, Positive Selection, 1 Million
Human Cord Blood CD34+ Stem/Progenitor Cells, Cryopreserved, 1 Million 2C-101
人脐带血 CD34+ 干/祖细胞,冷冻保存,100 万个 2C-101
Human CD34+ Progenitor Cells from Mobilized Peripheral Blood, Positive Selection, 1 Million
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 1 Million 2M-101C
Human CD34+ Progenitor Cells from Mobilized Peripheral Blood, Positive Selection, 1 Million
Fresh Human Leukopak – Neupogen® Mobilized (Day 5 Collection, Full Bag) 3Y-300
Fresh Human Leukopak – Neupogen® Mobilized(第 5 天系列,全袋)3Y-300

lonza PT-9502,PGM-2 前脂肪细胞生长培养基 SingleQuots™- 补充剂和生长因子

Catalog #: PT-9502
PGMTM-2 SingleQuotsTM Kit
Overview

Product Overview

PGMTM -2 前脂肪细胞生长培养基-2 BulletKitTM 培养基提供增殖培养基和分化培养基,保证诱导分化成成熟的、功能活跃的脂肪细胞; 每瓶 PGM-2TM BulletKitTM 培养基都含有足够的分化培养基,可将 1000 万个前脂肪细胞 [10 个完整的 96 孔板] 完全分化为成熟的功能活跃的脂肪细胞。 PGMTM-2 培养基以 BulletKitTM 培养基(目录号 PT-8002)的形式提供,其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。 基础培养基(PGMTM-2 基础培养基;目录号 PT-8202)和必要的补充剂(PGMTM-2 SingleQuotTM 试剂盒;目录号 PT-9502)也可以单独购买。

lonza CC-3165,原代细胞和干细胞,PrEBM™ 前列腺上皮细胞生长基础培养基

Catalog #: CC-3165
PrEBMTM Prostate Epithelial Basal Medium

Product Overview

PrEBMTM 是一种前列腺上皮细胞生长基础培养基,单独出售。 这可以与 1 套 CC-4177(前列腺上皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子)一起使用。 由基础培养基和补充剂组成的完整 PrEGMTM BulletKitTM 可以使用目录号 CC-3166 一起购买。

PrEGMTM BulletkitTM,前列腺上皮细胞生长培养基专为人前列腺上皮细胞的维持和扩增而配制。 PrEGMTM 培养基不含血清。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products
PrEBM™ Prostate Epithelial Cell Growth Basal Medium
PrEC – Human Prostate Epithelial Cells CC-2555

PrEC – 人前列腺上皮细胞 CC-2555

PrEBM™ Prostate Epithelial Cell Growth Basal Medium
PrEGM™ Prostate Epithelial Cell Growth Medium BulletKit™ CC-3166
PrEGM™ 前列腺上皮细胞生长培养基 BulletKit™ CC-3166

lonza CC-2505,HMVEC-dNeo人真皮微血管内皮细胞,新生儿,单一供体

Catalog #: CC-2505
HMVEC-d Neo, Dermal Microvascular Endothelial, Neonatal, Single donor
Overview

Product Overview

真皮微血管内皮细胞包括从成人或新生儿包皮的皮肤组织内的小血管中分离出来的细胞。培养的细胞纯度≥90%,并专门富集真皮 LEC 和 BEC。来自新生儿或成人的真皮微血管细胞 (HMVEC-D) 是血液 (HMVEC-DB1) 和淋巴 (HMVEC-DLy) 内皮细胞的混合群体。

成体细胞用“广告”表示;新生细胞用“Neo”表示。汇集和单一捐助者都可用。通过联系我们的科学支持团队,可以获得更多的捐赠者特征。 CloneticsTM 内皮细胞和培养基方便易用,使研究人员能够专注于结果。 CloneticsTM 细胞、培养基和试剂一起经过质量测试,保证作为一个完整的细胞系统提供最佳性能。

冷冻保存的细胞在第三代运输。冷冻保存的真皮微血管内皮细胞通过 15 次群体倍增(CC-2543、CC-2505 和 CC-2516)和 12 次群体倍增(CC-2810、CC-2811、CC-2812 和 CC-2813)保证,表达 CD31/105 ,von Williebrand 因子 VIII,对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products

HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543

HMVEC-dAd – 人真皮微血管内皮细胞 – 成人 CC-2543

HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor
NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504
NHEM-Neo – 人表皮黑色素细胞,新生儿 CC-2504

lonza CC-4193,OGM™ 成骨细胞生长培养基 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4193
OGMTM Osteoblasts Growth SingleQuotsTM Kit
lonza CC-4193,OGM™ 成骨细胞生长培养基 SingleQuots™ 补充剂和生长因子

Product Overview

OGMTM SingleQuotTM 试剂盒是单独出售的组件,不包括基础培养基。 这些是 OGMTM 成骨细胞生长培养基 BulletKitTM(目录号 CC-3207)的一部分。 由基础培养基和补充剂组成的完整 OGMTM BulletKitTM 可以使用目录号 CC-3207 一起购买。

CloneticsTM OGMTM 成骨细胞生长培养基专为人类成骨细胞的生长和扩增而配制。 该试剂盒含有 10% 的血清。 OGMTM 分化补充剂也包括在内,建议用于分化测定。 该试剂盒包装方便,包括含有生长和分化补充剂的基础培养基,用于成骨细胞的完全培养

Benefits

Applications:

  • In vitro bone models
  • Mineralization
  • Osteoporosis
  • Gene expression
  • Hormone treatments
  • Osteopenia
  • Paget’s Disease

Content & Storage

Content

1 x Bottle FBS 50.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL

Related Products
OGM™ Osteoblast Growth Medium SingleQuots™ Supplements and Growth Factors
OGM™ Osteoblast Growth Medium BulletKit™ CC-3207

OGM™ 成骨细胞生长培养基 BulletKit™ CC-3207

OGM™ Osteoblast Growth Medium SingleQuots™ Supplements and Growth Factors
NHOst – Human Osteoblasts CC-2538
 NHOst – 人类成骨细胞 CC-2538

lonza R-CXAS-520,原代细胞和干细胞,大鼠脑皮质星形胶质细胞

Catalog #: R-CXAS-520
Cryopreserved ampule of Rat Brain Cortex Astrocytes containing ≥ 1 million cells

Product Overview

即用型 CloneticsTM 大鼠脑皮层星形胶质细胞从大鼠脑中获得,传代一次并制备成细胞悬液,以便在干冰上运输。

每个小瓶包含大约 100 万个细胞。 星形胶质细胞可以很容易地解冻和培养。 星形胶质细胞可以收获并重新铺板或冷冻储存以备后用。 细胞在一夜之间运送。

推荐介质:AGMTM BulletKitTM (CC-3186)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

1 x Cryopreserved ampule of Rat Brain Cortex Astrocytes containing ≥ 1 million cells

Related Products

Rat Brain Cortex Astrocytes
AGM™ Astrocyte Growth Medium BulletKit™ CC-3186

AGM™ 星形胶质细胞生长培养基 BulletKit™ CC-3186

Rat Brain Cortex Astrocytes
Rat Microglia R-G-535
Rat Brain Cortex Astrocytes
Rat Brain Cortex Astrocytes R-CXAS-520

大鼠脑皮质星形胶质细胞 R-CXAS-520

Rat Brain Cortex Astrocytes
Rat Brain Striatum Astrocytes R-CPAS-522
Rat Brain Cortex Astrocytes
Rat Brain Hippocampus Astrocytes R-HIAS-521
Rat Brain Cortex Astrocytes
Rat Brain Cx-Hi-Cp Mixed Astrocytes R-ASM-530

大鼠脑 Cx-Hi-Cp 混合星形胶质细胞 R-ASM-530

Rat Brain Cortex Astrocytes
NHA – Human Astrocytes CC-2565
Rat Brain Cortex Astrocytes
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
Rat Brain Cortex Astrocytes
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
Rat Brain Cortex Astrocytes
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
Rat Brain Cortex Astrocytes
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

Rat Brain Cortex Astrocytes
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

Rat Brain Cortex Astrocytes
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Rat Brain Cortex Astrocytes
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza 00196979,D-HBE-CF-患病的人支气管/气管上皮细胞-囊性纤维化

Catalog #: 00196979
D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells from donors having Cystis Fibrosis

Product Overview

囊性纤维化是一种会导致在肺部、消化道和身体其他部位积聚粘稠粘液的疾病。 它是儿童和年轻人最常见的慢性肺部疾病之一。 这种疾病是影响美国高加索人的最常见、最致命的遗传性疾病。 疾病最终恶化到该人残疾的程度。 如今,能够活到成年的 CF 患者的平均寿命约为 37 岁,在过去的 30 年中显着增加。

来自囊性纤维化供体的冷冻保存的患病支气管上皮细胞的支原体、细菌、酵母和真菌检测呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。

筛选某些批次的特定基因突变。 如需更多信息,请联系我们的科学支持团队。

为购买的每个细胞批次提供分析证书。

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of D-HBE-CF containing ≥500,000 cells

Related Products
D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis
NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S

NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S

D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170
BEGM™ 支气管上皮细胞生长培养基 BulletKit™ CC-3170

lonza 00194911S,D-HBE-As-患病的人支气管/气管上皮细胞-B-ALI™ 培养的哮喘

Catalog #: 00194911S
DHBE-As,来自患有哮喘的供体的支气管上皮细胞,用于 B-ALI™(支气管气液界面)细胞培养

Product Overview

哮喘是一种气道炎症性疾病,可导致患者出现喘息、气短、胸闷和咳嗽。严重程度因个体患者而异,对药物治疗的反应也是如此。嗜酸性粒细胞与支气管上皮细胞的粘附以及气道平滑肌和上皮下成纤维细胞的相互作用在哮喘病理学中起关键作用。在哮喘发作期间,细支气管平滑肌组织可以收缩和减少气道中的空气流动。炎症或粘液分泌过多可进一步减少气流量。

冷冻保存的患病支气管上皮细胞的支原体、细菌、酵母菌和真菌检测呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。我们的目录号 00194911S 适用于气液界面研究,并保证促进气道上皮的完全分化,通过形成具有良好屏障功能(跨上皮阻力)、分泌表型(粘蛋白分泌)和纤毛发生的极化上皮表达。分化通常会在培养的第 22 天发生。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma for B-ALI™ Culture
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma for B-ALI™ Culture
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911
D-HBE-As – 患病的人支气管/气管上皮细胞 – 哮喘 00194911

lonza PT-3102A,原代细胞和干细胞,hMSC 脂肪生成维持培养基

Catalog #: PT-3102A

用于间充质干细胞成脂分化的维持培养基

Product Overview

脂肪生成维持培养基是 hMSC 脂肪生成分化 BulletKitTM 的一个组成部分,可在此处单独购买。

干细胞具有分化成包括脂肪细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 成脂分化 BulletKitTM 培养基是一种含血清的培养基系统,旨在诱导人骨髓来源的间充质干细胞在短短 19 天内成脂分化成成熟的、功能活跃的脂肪细胞。 然后可以通过多种方法验证分化,包括使用 Lonza 的 AdipoRedTM 脂肪生成分析试剂,该试剂提供与脂肪生成分化量相关的定量值。

Partial List of Cells Differentiated with hMSC Adipogenic Differentiation BulletKitTM Medium*:

  • Human bone marrow derived stem cells
  • C57BL/6 mice bone marrow derived stem cells
  • human adipose derived stem cells
  • Human periodontal ligament derived stem cells
  • Human hamstring tendon derived stem cells

hMSC 成脂分化 BulletKitTM 培养基提供诱导培养基和维持培养基,保证诱导人骨髓间充质干细胞成脂分化成成熟的、功能活跃的脂肪细胞;每瓶 hMSC 脂肪分化 BulletKitTM 培养基都含有足够的诱导培养基和维持培养基,可将大约 300 万个人骨髓来源的间充质干细胞 [6 孔板中大约 15 个孔] 完全分化为成熟的功能活跃的脂肪细胞。 hMSC 脂肪分化培养基以 BulletKitTM 培养基(目录号 PT-3004)的形式提供,其中包括用于诱导和维持的基础培养基以及用于脂肪形成诱导和维持人骨髓间充质干细胞的必要补充剂。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助,请联系 Lonza Scientific Support。

*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用,因此只能保证这种培养基与这些细胞一起成功使用。

lonza 2M-101B,原代细胞和干细胞,人骨髓 CD34+ 祖细胞

Catalog #: 2M-101B

来自骨髓的人 CD34+ 祖细胞的冷冻保存安瓿,含有 ≥ 500,000 个细胞。 使用阳性免疫磁性选择从单核细胞中分离细胞。

Product Overview

Bone marrow CD34+ cells contain hematopoietic stem and progenitor cells and are known to differentiate into all the various blood cell types.

Purity and Viability You Can Count On

Lonza骨髓CD34+细胞通过阳性免疫磁分离从骨髓单个核细胞(MNC)中分离出来,保证纯度≥90%。根据订购的产品,它们保证每个安瓿含有≥100,000、≥300,000、≥500,000、≥100 万或 ≥200 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、丙型肝炎和乙型肝炎。为购买的每个细胞批次提供分析证书。提供各种大小的新鲜骨髓 CD34+ 细胞,每个供体至少需要两瓶。有关新鲜细胞的尺寸和价格信息,请联系客户服务。

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能(根据应用可能需要额外的细胞因子) 也可以接受含有血清的替代培养基。有关含血清培养基的建议,请联系科学支持。

复杂的研究通常需要灵活性。这就是为什么我们提供分离和纯化的骨髓 CD34+ 细胞以及未加工的骨髓,以防您需要分离自己的细胞。骨髓 CD34+ 细胞非常适用于需要来自单个供体的大量细胞的研究或调查不同年龄、性别或种族供体的细胞差异的研究。但是,如果您的研究需要更多幼稚细胞,脐带血 CD34+ 细胞可能是更好的选择。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

Benefits

  • Ready to use
  • Wide donor variety
  • Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34+ cells)

Content & Storage

Content

1 x Cryopreserved ampule of Human Bone Marrow CD34+ containing ≥ 500,000 cells

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Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
IMDM with L-Glutamine and HEPES 12-722F

含有 L-谷氨酰胺和 HEPES 12-722F 的 IMDM

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞,500 万个 2M-302

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒(25 次测试)LT07-218

lonza CC-4177,PrEGM™ 前列腺上皮细胞生长培养基 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4177
PrEGMTM Prostate Epithelial SingleQuotsTM Kit

Product Overview

PrEGMTM SingleQuotTM 试剂盒是单独出售的组件,不包括基础培养基。 这些是 PrEGMTM 前列腺上皮细胞生长培养基 BulletKitTM(目录号 CC-3166)的一部分。 由基础培养基和补充剂组成的完整 PrEGMTM BulletKitTM 可以使用目录号 CC-3166 一起购买。

PrEGMTM 前列腺上皮细胞生长培养基专为人前列腺上皮细胞的维持和扩增而配制。 PrEGMTM 培养基不含血清。    

Content & Storage

Content

1 x Orange Cap Vial with BPE 2.00 mL
1 x Amber Vial with Triiodothyronine 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Amber Vial with Retinoic Acid 0.50 mL

Related Products
PrEGM™ Prostate Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors
PrEC – Human Prostate Epithelial Cells CC-2555

PrEC – 人前列腺上皮细胞 CC-2555

PrEGM™ Prostate Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors
PrEGM™ Prostate Epithelial Cell Growth Medium BulletKit™ CC-3166
 PrEGM™ 前列腺上皮细胞生长培养基 BulletKit™ CC-3166

lonza00195275S,D-HBE-COPD,患病的人支气管/气管上皮细胞,B-ALITM 的慢性阻塞性肺病

Catalog #: 00195275S
DHBE-COPD, Bronchial Epithelial Cells from donors with COPD for B-ALI™ (Bronchial Air Liquid Interface) cell culture
DHBE-COPD,来自 COPD 供体的支气管上皮细胞用于 B-ALI™(支气管气液界面)细胞培养

Product Overview

Chronic bronchitis is marked by long-term cough and excess mucous. Emphysema leads to the destruction of the lungs over time. Most people with COPD have a combination of both conditions. Usually COPD is associated with bronchial epithelium changes.

Cryopreserved diseased human bronchial epithelial cells from donors diagnosed with COPD are screened for air liquid interface differentiation. These cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Our catalog number 00195275S are suitable for air-liquid interface studies and are guaranteed to promote full differentiation of the airway epithelium expressed by the formation of a polarized epithelium with good barrier function (transepithelial resistance), secretory phenotype (mucin secretion) and ciliogenesis. Differentiation usually will occur by day 22 in culture.

慢性支气管炎的特点是长期咳嗽和粘液过多。 随着时间的推移,肺气肿会导致肺部受损。 大多数患有慢性阻塞性肺病的人同时存在这两种情况。 通常COPD与支气管上皮变化有关。

对来自诊断为 COPD 的供体的冷冻保存的患病人支气管上皮细胞进行气液界面分化筛选。 这些细胞的支原体、细菌、酵母和真菌检测呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

我们的目录号 00195275S 适用于气液界面研究,并保证促进气道上皮的完全分化,通过形成具有良好屏障功能(跨上皮阻力)、分泌表型(粘蛋白分泌)和纤毛发生的极化上皮表达。 分化通常会在培养的第 22 天发生。

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot
  • 所有细胞的支原体、细菌、酵母和真菌检测均为阴性
    并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
    为每个细胞批次提供分析证书

Content & Storage

Content

1 x Cryopreserved ampule containing ≥ 500,000 cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

D-HBE-COPD &ndash; Diseased Human Bronchial/Tracheal Epithelial Cells &ndash; COPD for B-ALITM
NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture CC-2540S
NHBE – 用于 B-ALITM 培养的人支气管/气管上皮细胞 CC-2540S
D-HBE-COPD &ndash; Diseased Human Bronchial/Tracheal Epithelial Cells &ndash; COPD for B-ALITM
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞
D-HBE-COPD &ndash; Diseased Human Bronchial/Tracheal Epithelial Cells &ndash; COPD for B-ALITM
D-SAEC-COPD – Diseased Small Airway Epithelial Cells – COPD CC-2934
D-SAEC-COPD – 患病小气道上皮细胞 – COPD CC-2934
D-HBE-COPD &ndash; Diseased Human Bronchial/Tracheal Epithelial Cells &ndash; COPD for B-ALITM
D-HBE-As – Diseased Human Bronchial/Tracheal Epithelial Cells – Asthma 00194911
D-HBE-As – 患病的人支气管/气管上皮细胞 – 哮喘 00194911
D-HBE-COPD &ndash; Diseased Human Bronchial/Tracheal Epithelial Cells &ndash; COPD for B-ALITM
D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis 00196979
D-HBE-CF – 患病的人支气管/气管上皮细胞 – 囊性纤维化 00196979

lonza,lonzaPT-3924,原代细胞和干细胞,hMSC – 人间充质干细胞成骨分化基础培养基

Catalog #: PT-3924
hMSC Osteogenic Differentiation Basal Medium, 170 mL
Instructions, SDS, CoA

Safety Data Sheets (SDS)

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SDS – German
SDS – Italian
SDS – Norwegian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza00195130,原代细胞和干细胞,KBM™ Gold 角质形成细胞生长基础培养基,不含钙和酚红

Catalog #: 00195130
KBMTM Gold Keratinocyte Basal Medium, Calcium-free
KBMTM Gold 角质形成细胞基础培养基,无钙

Product Overview

KBMTM Gold is a calcium-free keratinocyte basal medium sold separately. This can be used with 1 kit of 192152 (keratinocyte cell growth medium SingleQuotsTM Supplements and Growth Factors). The full KGMTM Gold Calcium Free BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number 195769.

KGMTM Gold BulleKitTM is a new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures

KBMTM Gold 是一种不含钙的角质形成细胞基础培养基,单独出售。 这可以与 1 套 192152(角质形成细胞生长培养基 SingleQuotsTM 补充剂和生长因子)一起使用。 由基础培养基和补充剂组成的完整 KGMTM Gold Calcium Free BulletKitTM 可以使用目录号 195769 一起购买。

KGMTM Gold BulleKitTM 是一种新的稳健且多功能的无血清培养基配方,适用于高密度和低密度 NHEK-正常人表皮角质形成细胞培养物

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor 00192907
NHEK-Neo – 人类表皮角质形成细胞 – 新生儿,单一供体 00192907
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627
NHEK-Ad – 人表皮角质形成细胞,成人,单一供体 00192627
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled 00192906
NHEK-Neo – 人表皮角质形成细胞,新生儿,汇集 00192906
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II CC-2926
D-HEK-Ad – 患病的人表皮角质形成细胞,成人 – II 型糖尿病 CC-2926
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free
KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free 00195769
KGM™ Gold 角质形成细胞生长培养基 BulletKit™,无钙 00195769
Data
KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free

Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.

The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.

lonza,lonza CC-4540,InMyoFib CC-2902,人肠上皮和肌成纤维细胞组合

货号: CC-4540
Intestinal Epithelial and Myofibroblast Cell Combo

Product Overview

InEpC’s are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures.  In combination with human intestinal myofibroblasts (InMyoFib), InEpC are able to form very tight cell monolayer, representing a unique in vitro system to model human intestinal homeostasis.

Human intestinal epithelial cells (InEpC) are of pivotal importance in the digestion and absorption of nutrients and the protection of the organism from bacteria and toxins found in the intestine. The intestinal epithelium is a highly organized cellular system maintained in a dynamic steady state by cells in proliferation and differentiation, which die and exfoliate into the lumen. Intestinal cells are often difficult to isolate due to contamination issues and usually have to be cultured and used for assays immediately after isolation from tissue.

Cryopreserved InEpCs are sold at P0, express cytokeratins 8/18 at ≥ 90%, contain 0.8M viable cells / vial and have a seeding efficiency ≥ 50% coverage at certain cell density (see instructions).  Their life span is, post plating, that the cells can be used on days 5-7, and they maintain their properties for approximately 5 days after that (using CloneticsTM reagents & protocol).  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

InEpC 是真正的原代细胞,代表绒毛(肠细胞、杯状细胞和肠内分泌细胞)和隐窝结构。与人类肠道肌成纤维细胞 (InMyoFib) 结合,InEpC 能够形成非常紧密的细胞单层,代表了一种独特的体外系统来模拟人类肠道稳态。

人类肠道上皮细胞 (InEpC) 在营养物质的消化和吸收以及保护生物体免受肠道中发现的细菌和毒素的侵害方面至关重要。肠上皮是一种高度组织化的细胞系统,由增殖和分化中的细胞维持在动态稳定状态,这些细胞死亡并脱落进入管腔。由于污染问题,肠细胞通常难以分离,并且通常必须在从组织中分离后立即进行培养和用于检测。

冷冻保存的 InEpC 以 P0 销售,以 ≥ 90% 表达细胞角蛋白 8/18,包含 0.8M 活细胞/小瓶,并且在一定细胞密度下具有 ≥ 50% 的接种效率(见说明)。它们的寿命是,电镀后,细胞可以在第 5-7 天使用,并且在此之后它们保持其特性大约 5 天(使用 CloneticsTM 试剂和协议)。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
CloneticsTM Human Intestinal Myofibroblasts

H-InMyoFib share phenotypic characteristics of both fibroblasts and smooth muscle cells but do not express smooth muscle markers such as smoothelin, caldesmon, or (in the normal colon and small intestine) desmin. They play a fundamental role in growth and development of intestine, immunology, inflammation, fibrosis, water and electrolyte transport, contraction and wound healing. They are a useful tool in general gastrointestinal research.

Cryopreserved InMyoFib are guaranteed through 15 population doublings and stain ≥70% postitive for alpha smooth muscle actin and negative (≤10%) for desmin after thaw. They are cryopreserved at passage 2 with ≥500,000 cells per vial.  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

H-InMyoFib 具有成纤维细胞和平滑肌细胞的表型特征,但不表达平滑肌标志物,如平滑素、caldesmon 或(在正常结肠和小肠中)结蛋白。 它们在肠道的生长和发育、免疫学、炎症、纤维化、水和电解质运输、收缩和伤口愈合中发挥着重要作用。 它们是一般胃肠道研究的有用工具。

冷冻保存的 InMyoFib 通过 15 次群体倍增和 α 平滑肌肌动蛋白染色≥70% 阳性和解冻后结蛋白阴性 (≤10%) 得到保证。 它们在第 2 代冷冻保存,每瓶≥500,000 个细胞。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule of Intestinal Epithelial Cells with ≥800,000 cells/vial
1 x Cryopreserved Ampule of Intestinal Myofibroblasts with ≥500,000 cells/vial

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

Human Intestinal Epithelial and Myofibroblast Cell Combo
InMyoFib – Human Intestinal Myofibroblasts CC-2902
InMyoFib – 人类肠道肌成纤维细胞 CC-2902
Human Intestinal Epithelial and Myofibroblast Cell Combo
InEpC – Human Intestinal Epithelial Cells CC-2931
InEpC – 人肠上皮细胞 CC-2931
Human Intestinal Epithelial and Myofibroblast Cell Combo
RPTEC – Human Renal Proximal Tubule Epithelial Cells CC-2553
RPTEC – 人肾近端小管上皮细胞 CC-2553
Human Intestinal Epithelial and Myofibroblast Cell Combo
NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540
NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

lonza PT-4120,hMSC 人间充质干细胞成骨分化培养基 SingleQuotsTM 添加剂和生长因子

Catalog #: PT-4120
hMSC Osteogenic Differentiation SingleQuotsTM Kit
hMSC 成骨分化 SingleQuotsTM 试剂盒
Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonzaCC-3217,原代细胞和干细胞,CBM™ 软骨细胞生长基础培养基, CGM™ CC-3216

货号: CC-3217
CBMTM Chondrocyte Growth Basal Medium
CBMTM 软骨细胞生长基础培养基

Product Overview

CBMTM is a Chondrocyte cell growth basal medium sold separately. This can be used with 1 kit of CC-4409 (chondrocyte cell growth medium SingleQuotsTM Supplements and Growth Factors). The full CGMTM BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number CC-3216.

  • CGMTM Chondrocyte Growth Medium is specially designed for expansion of chondrocytes. The BulletKitTM is conveniently packaged with basal medium and supplements included for the growth of chondrocytes.
  • CDMTM Chondrocyte Differentiation Medium is also available and sold separately for differentiation of human chondrocytes

CBMTM 是一种软骨细胞生长基础培养基,单独出售。 这可以与 1 套 CC-4409(软骨细胞生长培养基 SingleQuotsTM 补充剂和生长因子)一起使用。 由基础培养基和补充剂组成的完整 CGMTM BulletKitTM 可以使用目录号 CC-3216 一起购买。

CGMTM 软骨细胞生长培养基专为软骨细胞的扩增而设计。 BulletKitTM 方便地与基础培养基和用于软骨细胞生长的补充剂一起包装。
CDMTM 软骨细胞分化培养基也可单独购买和出售,用于分化人软骨细胞

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

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Related Products

CBM™ Chondrocyte Growth Basal Medium
NHAC-kn – Human Knee Articular Chondrocytes CC-2550
NHAC-kn – 人膝关节软骨细胞 CC-2550
CBM™ Chondrocyte Growth Basal Medium
CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225
CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225
CBM™ Chondrocyte Growth Basal Medium
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216
 CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

lonza,lonza MCCP01,人肝细胞,可电镀 HUCPG,小鼠 CD-1 肝细胞

Catalog #: MCCP01
Cryopreserved CD-1 Mouse Hepatocytes, Plateable format
冷冻保存的 CD-1 小鼠肝细胞,平板形式

Product Overview

All our plateable cryopreserved primary animal hepatocytes are specifically pre tested for metabolic activity of general Phase I and Phase II metabolism in suspension. They are tested for healthy morphology lasting 72 hours in monlayer sandwich culture.

我们所有的可平板冷冻保存的原代动物肝细胞都经过专门的预测试,以了解悬浮液中一般 I 期和 II 期代谢的代谢活性。 它们在单层夹层培养中经过 72 小时的健康形态测试。

Applications

  • Drug metabolism in plated monolayer format
  • Species-species comparisons
  • Genomics

Content & Storage

Content

Cryo vial, > 5 million viable cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Related Products

Mouse CD-1 Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable HUCPG
人肝细胞,冷冻保存,可电镀 HUCPG
Mouse CD-1 Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI

人肝细胞,冷冻保存,可电镀和相互作用合格的 HUCPI

Mouse CD-1 Hepatocytes, Cryopreserved, Plateable

lonza3W-300,lonza2W-300,原代细胞和干细胞,新鲜人 CD8+ 细胞毒性 T 细胞

货号: 3W-300
Human CD8+ cytotoxic T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 10 million cells, fresh
人 CD8+ 细胞毒性 T 细胞,使用负免疫磁性选择从外周血中分离,≥ 1000 万个细胞,新鲜

Product Overview

Leukapheresed CD8+ Cytotoxic T cells are negatively selected from MNC using an indirect immunomagnetic CD8+ T cell labeling system. Cells are shipped in a centrifuge tube. Cell purity is determined using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Manufactured by AllCells® Additional shipping charges apply for fresh, human origin products

使用间接免疫磁性 CD8+ T 细胞标记系统从 MNC 中阴性选择 Leukapheresed CD8+ 细胞毒性 T 细胞。 细胞在离心管中运输。 使用 FACS 测定法确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。 由 AllCells® 制造 新鲜的人源产品需支付额外运费

Content & Storage

Content

1 vial containing ≥ 10 million fresh cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

Related Products

Fresh Human CD8+ Cytotoxic T Cells
Human Peripheral Blood CD8+ T Cells, Cryopreserved, 10 million cells 2W-300
人外周血 CD8+ T 细胞,冷冻保存,1000 万个细胞 2W-300

lonza,lonzaCC-4398,原代细胞和干细胞,抗坏血酸0.5 mL

货号: CC-4398
Ascorbic Acid, 0.5 mL

Product Overview

Ascorbic Acid is an important component in various primary cell culture media kits. It is included in various BulletKits. Ascorbic Acid is also sold separately at a concentration of 70 mM

抗坏血酸是各种原代细胞培养基试剂盒中的重要成分。 它包含在各种 BulletKits 中。 抗坏血酸也单独出售,浓度为 70 mM

Content & Storage

Content

1 x Vial Containing 70 mM Ascorbic Acid 0.5 mL

Instructions, SDS, CoA

Ascorbic Acid, 0.5 mL

Safety Data Sheets (SDS)

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lonza,lonzaCC-2813,HMVEC-dBl-Neo – 人真皮血液微血管内皮细胞 – 新生儿

货号: CC-2813
HMVEC-dBlNeo, Dermal Blood Endothelial Cells, Neonatal
HMVEC-dBlNeo,真皮血液内皮细胞,新生儿

Product Overview

Dermal Microvascular Endothelial Cells include cells isolated from small vessels within skin tissue from either adults or neonatal foreskins.  Cells are cultured to be ≥90% pure and specifically enriched for dermal LECs and BECs. Dermal microvascular cells, from either neonates or adults (HMVEC-D) are a mixed population of both blood (HMVEC-DBl) and lymphatic (HMVEC-DLy) endothelial cells.

Adult cells are designated with an ‘Ad’; neonate cells are designated with ‘Neo’.  Both pooled and single donors are available. Additional donor characteristics may be available by contacting our Scientific Support Team. CloneticsTM Endothelial Cells and Media are convenient and easy to use, allowing the researcher to focus on results. CloneticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system. Cryopreserved cells are shipped in third passage.

Cryopreserved Dermal Microvascular Endothelial Cells are guaranteed through 15 population doublings (CC-2543, CC-2505 & CC-2516) and 12 population doublings (CC-2810, CC-2811, CC-2812 & CC-2813) , express CD31/105, von Williebrand Factor VIII, and are positive for acetyated low density lipoprotein uptake.  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

真皮微血管内皮细胞包括从成人或新生儿包皮的皮肤组织内的小血管中分离出来的细胞。培养的细胞纯度≥90%,并专门富集真皮 LEC 和 BEC。来自新生儿或成人的真皮微血管细胞 (HMVEC-D) 是血液 (HMVEC-DB1) 和淋巴 (HMVEC-DLy) 内皮细胞的混合群体。

成体细胞用“广告”表示;新生细胞用“Neo”表示。汇集和单一捐助者都可用。通过联系我们的科学支持团队,可以获得更多的捐赠者特征。 CloneticsTM 内皮细胞和培养基方便易用,使研究人员能够专注于结果。 CloneticsTM 细胞、培养基和试剂一起经过质量测试,保证作为一个完整的细胞系统提供最佳性能。冷冻保存的细胞在第三代运输。

冷冻保存的真皮微血管内皮细胞保证通过 15 次群体倍增(CC-2543、CC-2505 和 CC-2516)和 12 次群体倍增(CC-2810、CC-2811、CC-2812 和 CC-2813),表达 CD31/105 ,von Williebrand 因子 VIII,对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Related Products

HMVEC-dBl-Neo - Human Dermal Blood Microvascular Endothelial Cells - Neonatal
HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Single Donor CC-2505
HMVEC-dNeo – 人真皮微血管内皮细胞,新生儿,单供体 CC-2505
HMVEC-dBl-Neo - Human Dermal Blood Microvascular Endothelial Cells - Neonatal
NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504
 NHEM-Neo – 人表皮黑色素细胞,新生儿 CC-2504

lonza,lonza3M-103,原代细胞和干细胞,未加工的人骨髓, Fresh, 3 mL

Catalog #: 3M-103
Fresh Unprocessed Human Bone Marrow, 3 mL
新鲜未加工的人骨髓

Product Overview

Bone marrow aspirate is collected from healthy donors and contains 80 IU/mL (nominal) porcine heparin as an anticoagulant. The fresh tissue contains CD34+ hematopoietic stem and progenitor cells (HSPC) and mesenchymal stem/stromal cells (MSC). CD34+ HSPC have the potential to self-renew and differentiate into all the mature blood cell lineages. MSC have the potential to differentiate into several lineages including bone, cartilage, muscle, and fat. Cells are collected in a transport tube.

Manufactured by AllCells®

Additional shipping charges apply for fresh, human origin products

从健康供体收集的骨髓抽吸物含有 80 IU/mL(标称)猪肝素作为抗凝剂。 新鲜组织含有 CD34+ 造血干细胞和祖细胞 (HSPC) 和间充质干/基质细胞 (MSC)。 CD34+ HSPC 具有自我更新和分化成所有成熟血细胞谱系的潜力。 MSC 有可能分化成几个谱系,包括骨骼、软骨、肌肉和脂肪。 将细胞收集在运输管中。

由 AllCells® 制造

新鲜的人源产品需支付额外的运费

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased
  • 支原体、细菌、酵母菌和真菌检测呈阴性
    并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
    为购买的每个细胞批次提供分析证书 (CoA)

Content & Storage

Content

1 x Unprocessed Human Bone Marrow, 3 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza CC-4525,FGM™3 心脏成纤维细胞生长培养基-3 SingleQuots™ 补充剂和生长因子

货号: CC-4525
FGMTM-3 Cardiac Fibroblasts SingleQuotsTM Kit,FGMTM-3 心脏成纤维细胞 SingleQuotsTM 试剂盒
Overview

Product Overview

FGMTM -3 SingleQuotTM Kit are components sold separately and does not include the basal medium. These are part of the FGMTM -3 Fibroblast Growth Medium BulletKitTM (Catalog Number CC-4526) which consists of basal medium and supplements packaged together.

FGMTM-3 Fibroblast Growth Medium-3 was developed specifically for cardiac fibroblasts. FGMTM-3 Medium contains contains 10% serum.
FGMTM -3 SingleQuotTM 套件是单独出售的组件,不包括基础培养基。 这些是 FGMTM -3 成纤维细胞生长培养基 BulletKitTM(目录号 CC-4526)的一部分,它由包装在一起的基础培养基和补充剂组成。

FGMTM-3 Fibroblast Growth Medium-3 专为心脏成纤维细胞开发。 FGMTM-3 培养基含有 10% 血清。

 

Content & Storage

Content

1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 50.00 mL

Instructions, SDS, CoA

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Related Products

FGM™3 Cardiac Fibroblast Growth Medium-3 SingleQuots™ Supplements and Growth Factors
NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903,NHCF-A – 人心房心脏成纤维细胞 CC-2903
FGM™3 Cardiac Fibroblast Growth Medium-3 SingleQuots™ Supplements and Growth Factors
NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904
NHCF-V – 人心室心脏成纤维细胞 CC-2904
FGM™3 Cardiac Fibroblast Growth Medium-3 SingleQuots™ Supplements and Growth Factors
FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™ CC-4526
 FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™ CC-4526

lonza,lonza R-RET-508,原代细胞和干细胞,大鼠新生儿视网膜细胞,BulletKit™ CC-4461

货号: R-RET-508

Cryopreserved ampule of Rat Neonatal Retinal Cells containing ≥200,000 cells

含有≥200,000 个细胞的大鼠新生儿视网膜细胞冻存安瓿

Product Overview

CloneticsTM Neonatal Rat Retinal Cells [P3-4] are ready-to-use, high quality primary cells from Sprague Dawley rats. Cells are prepared by standardized methods and are ready for immediate culture upon thaw. Cells are cryopreserved with ≥ 0.2M viable cells per ampule.

Cells are characterized by immunostaining for neuron specific class III β-tubulin (Tuj-1), specific neuronal protein gene product (PGP 9.5), ganglion cell marker, Thy1.1 and GFAP.

Recommended mediumPNGMTM BulletKitTM

CloneticsTM 新生大鼠视网膜细胞 [P3-4] 是来自 Sprague Dawley 大鼠的即用型高质量原代细胞。 细胞通过标准化方法制备,解冻后即可立即培养。 细胞以每安瓿 ≥ 0.2M 活细胞冷冻保存。

细胞的特征在于对神经元特异性 III 类 β-微管蛋白 (Tuj-1)、特异性神经元蛋白基因产物 (PGP 9.5)、神经节细胞标记物、Thy1.1 和 GFAP 进行免疫染色。

推荐介质:PNGMTM BulletKitTM

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • General ophthalmic research
  • Posterior segment disease
  • Inflammation
  • Degeneration
  • Neoplasms
  • Drug delivery
  • Cell therapies
  • Toxicology and cytotoxicity
  • Gene expression

Content & Storage

Content

≥200,000 cryopreserved cells per ampule

Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Related Products

Rat Neonatal Retinal Cells
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461
PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Rat Neonatal Retinal Cells

lonza,lonza 00195406,原代细胞和干细胞,RtEBM™ 视网膜上皮细胞基础培养基

Catalog #: 00195406

Retinal Epithelial Cell Basal Medium, 200 mL

视网膜上皮细胞基础培养基,200 mL

Product Overview

RtEBMTM is a Retinal Epithelial Cell basal medium sold separately. This can be used with 1 kit of 195407 (retinal epithelial cell growth medium SingleQuotsTM Supplements and Growth Factors). The full RtEGMTM BulletKitTM consisting of basal medium and supplements can be purchased together using the catalog number 195409.

RtEGMTM Retinal Pigment Epithelial Cell Growth Medium is optimized and performance tested for growing Human RPE cells. It contains 2% serum.

RtEBMTM 是一种视网膜上皮细胞基础培养基,单独出售。 这可以与 1 套 195407(视网膜上皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子)一起使用。 可以使用目录号 195409 一起购买由基础培养基和补充剂组成的完整 RtEGMTM BulletKitTM。

RtEGMTM 视网膜色素上皮细胞生长培养基针对生长的人类 RPE 细胞进行了优化和性能测试。 它含有2%的血清。

Benefits

Applications:

  • Age-related macular degeneration
  • Retinitis pigmentosa
  • Diabetic retinopathy
  • Photoreceptor degeneration
  • Gene expression
  • Cellular interactions

Content & Storage

Content

1 x Bottle Basal Medium 200 mL

Instructions, SDS, CoA

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Related Products

RtEBM™ Retinal Epithelial Cell Basal Medium
RtEGM™ Retinal Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors 00195407
RtEGM™ 视网膜上皮细胞生长培养基 SingleQuots™ 添加剂和生长因子 00195407
RtEBM™ Retinal Epithelial Cell Basal Medium
H-RPE – Human Retinal Pigment Epithelial Cells 00194987
H-RPE – 人视网膜色素上皮细胞 00194987
RtEBM™ Retinal Epithelial Cell Basal Medium
RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™ 00195409
 RtEGM™ 视网膜色素上皮细胞生长培养基 BulletKit™ 00195409

lonza,lonza CC-3210,原代细胞和干细胞,NPBM™ 神经祖细胞基础培养基

货号: CC-3210
NPBMTM Medium, 200 mL

Product Overview

PoieticsTM Neural Progenitor Cell System contains Norman Human Neural Progenitor Cells (NHNP) and defined medium optimized for their growth and in vitro differentiation. This system may be used for experimental applications including drug development, neurotoxicity, neurogenesis, electrophysiology, neurotransmitter disorders, and central nervous system function.

The available defined neural progenitor media includes a growth medium BulletKitTM (CC-3209) as well as a differentiaion medium BulletKitTM (CC-3229).

PoieticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.

PoieticsTM 神经祖细胞系统包含诺曼人神经祖细胞 (NHNP) 和针对其生长和体外分化优化的确定培养基。 该系统可用于实验应用,包括药物开发、神经毒性、神经发生、电生理学、神经递质疾病和中枢神经系统功能。

可用的定义神经祖细胞培养基包括生长培养基 BulletKitTM (CC-3209) 以及分化培养基 BulletKitTM (CC-3229)。

PoieticsTM 细胞、培养基和试剂一起经过质量测试,保证作为一个完整的细胞系统提供最佳性能。

Content & Storage

Content

  •  Neural Progenitor Basal Medium (1 bottle)
Instructions, SDS, CoA

Safety Data Sheets (SDS)

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Related Products

NPBM™ Neural Progenitor Basal Medium
NHNP – Human Neural Progenitor Cells PT-2599
NHNP – 人类神经祖细胞 PT-2599

lonza,lonza CC-3229,原代细胞和干细胞,NPDM™ 神经祖细胞分化培养基 BulletKit™

货号: CC-3229
NPDM BulletKitTM
lonza,lonza CC-3229,原代细胞和干细胞,NPDM™ 神经祖细胞分化培养基 BulletKit™

Product Overview

PoieticsTM Neural Progenitor Cell System contains Norman Human Neural Progenitor Cells (NHNP) and defined medium optimized for their growth and in vitro differentiation. This system may be used for experimental applications including drug development, neurotoxicity, neurogenesis, electrophysiology, neurotransmitter disorders, and central nervous system function.

The available defined neural progenitor media includes a growth medium BulletKitTM (CC-3209) as well as a differentiaion medium BulletKitTM (CC-3229).

PoieticsTM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.

Instructions, SDS, CoA

PoieticsTM 神经祖细胞系统包含诺曼人神经祖细胞 (NHNP) 和针对其生长和体外分化优化的确定培养基。 该系统可用于实验应用,包括药物开发、神经毒性、神经发生、电生理学、神经递质疾病和中枢神经系统功能。

可用的定义神经祖细胞培养基包括生长培养基 BulletKitTM (CC-3209) 以及分化培养基 BulletKitTM (CC-3229)。

PoieticsTM 细胞、培养基和试剂一起经过质量测试,保证作为一个完整的细胞系统提供最佳性能。

说明书、SDS、CoA

Safety Data Sheets (SDS)

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Related Products
NPDM™ Neural Progenitor Differentiation Medium BulletKit™
NHNP – Human Neural Progenitor Cells PT-2599
NHNP – 人类神经祖细胞 PT-2599

lonza,lonza RSCP01,原代细胞和干细胞,大鼠 Sprague Dawley 肝细胞

Catalog #: RSCP01

Rat Plateable Cryopreserved Hepatocytes are specifically tested for healthy morphology lasting 72 hours in monolayer sandwich culture.

大鼠可平板冻存肝细胞在单层夹心培养中经过 72 小时的健康形态学专门测试。

Product Overview

Cryopreserved Sprague Dawley Rat Hepatocytes, plateable qualified  are specifically pre-tested for metabolic activity of general Phase I and Phase II metabolism in suspension. They are tested for healthy morphology lasting 72 hours in monolayer sandwich culture.

Many batches are talso ested for spheroid formation capacity.  Look for the Verified for SpheroidsTMcharacteristic on each CofA

冷冻保存的 Sprague Dawley 大鼠肝细胞,可电镀合格,专门针对悬浮液中一般 I 期和 II 期代谢的代谢活性进行了预测试。 它们在单层夹层培养中经过 72 小时的健康形态测试。

还评估了许多批次的球体形成能力。 在每个 CofA 上查找 Verified for SpheroidsTMcharacteristic

Benefits

  • Plateable
  • >5Million cells/vial
  • Characterized for general drug metabolism

Applications

  • Drug metabolism in plated monolayer format
  • species-species comparisons
  • genomics

Content & Storage

Content

Cryo vial, > 5 million viable cells

Instructions, SDS, CoA

Related Products

Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞,冷冻保存,可电镀 HUCPG

Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable
Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI

Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable

人肝细胞,冷冻保存,可电镀和相互作用合格的 HUCPI
Rat Sprague Dawley Hepatocytes, Cryopreserved, Plateable

lonza,lonza M-IFB-482,原代细胞和干细胞,辐照小鼠胚胎成纤维细胞 (iMEF)

Catalog #: M-IFB-482

Cryopreserved ampule of Irradiated Mouse Embryonic Fibroblasts containing ≥2 million cells

冷冻保存的辐照小鼠胚胎成纤维细胞安瓿,含有≥200万个细胞

Product Overview

Irradiated Mouse primary embryonic fibroblasts (iMEF) are dissociated from day 14 and 15 CD1 mouse embryos, expanded and then inactivated using gamma radiation.

Each vial contains at least 2 million cells. iMEF are convenient and easy to use “feeder cells” commonly co-cultured with embryonic and pluripotent stem cell cultures.

Recommended Media: Lonza’s DMEM High Glucose w/ L-Gln, 500 mL (Part 12-604F) containing 10% FBS (Sigma-Aldrich Part F6178)

经辐照的小鼠原代胚胎成纤维细胞 (iMEF) 从第 14 天和第 15 天的 CD1 小鼠胚胎中分离出来,扩增然后使用伽马辐射灭活。

每个小瓶包含至少 200 万个细胞。 iMEF 是方便且易于使用的“饲养细胞”,通常与胚胎和多能干细胞培养物共同培养。

推荐培养基:Lonza’s DMEM High Glucose w/ L-Gln,500 mL(第 12-604F 部分),含 10% FBS(Sigma-Aldrich 第 F6178 部分)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Serve as “feeder cell” monolayer in embryonic (ES) and induced pluripotent (iPSC) stem cell cultures
  • Secrete important growth factors into culture medium to support ES and iPSC undifferentiated state
  • Act as cellular matrix to support ES cell growth
  • 在胚胎 (ES) 和诱导多能 (iPSC) 干细胞培养物中充当“饲养细胞”单层
    将重要的生长因子分泌到培养基中以支持 ES 和 iPSC 未分化状态
    作为细胞基质支持 ES 细胞生长

Content & Storage

Content

≥ 2 million cryopreserved, gamma irradiated cells per ampule

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

 

Related Products
Irradiated Mouse Embryonic Fibroblasts (iMEF)
HEPES Buffered Saline Solution – 100 ml CC-5022

HEPES 缓冲盐溶液 – 100 ml CC-5022

lonza,lonza CC-3146,lonza CC-4146,MsGM™ 系膜细胞生长培养基 BulletKit™

货号: CC-3146

***Limited time offer – Get 30% off with promocode PSTOCKUP on your order. Offer valid in US and select countries in Europe only until March 31, 2019.***

Culture system containing MsGMTM Mesangial Basal Medium (CC-3147) and MsGMTM SingleQuotsTM Supplements (CC-4146) required for growth of human mesangial cells.

包含人系膜细胞生长所需的 MsGMTM 系膜基础培养基 (CC-3147) 和 MsGMTM SingleQuotsTM 补充剂 (CC-4146) 的培养系统。

Product Overview

MsGMTM Mesangial Cell Growth Medium has been specially formulated for the maintenance and expansion of renal mesangial cells. MsGMTM contains 5% serum. The BulletKitTM consists of basal medium and supplements packaged together for convenience.

MsGMTM 系膜细胞生长培养基专为肾系膜细胞的维持和扩增而配制。 MsGMTM 含有 5% 的血清。 BulletKitTM 由基础培养基和补充剂组成,方便起见。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 25.00 mL

Instructions, SDS, CoA

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

 

 

Related Products
MsGM™ Mesangial Cell Growth Medium BulletKit™
MsBM™ Mesangial Cell Growth Basal Medium CC-3147
MsBM™ 系膜细胞生长基础培养基 CC-3147
MsGM™ Mesangial Cell Growth Medium BulletKit™
MsGM™ Mesangial Cell Growth Medium SingleQuots™ Supplements and Growth Factors CC-4146

MsGM™ 系膜细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4146

lonza,lonza CC-3103,原代细胞和干细胞,KBM™- 2 角质形成细胞基础培养基-2

Catalog #: CC-3103
KBMTM 2 Keratinocyte Basal Medium-2, 500 mL
KBMTM 2 角质形成细胞基础培养基-2,500 mL

Product Overview

KGM-2 BulletkitTM, also sold as separate basal medium and supplements

KGM-2 BulletkitTM,也作为单独的基础培养基和补充剂出售

Product Overview:

CloneticsTM KGMTM-2 Keratinocyte Growth Medium-2 has been optimized for the proliferation of keratinocytes in a serum-free environment and typically provides higher growth rates than KGMTM Medium.

CloneticsTM Media Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems. Media are supplied in several formats:

  • Basal Medium – contains no growth factors cytokines, or supplements
  • SingleQuotsTM Kit – growth factors, cytokines, and supplements
  • BulletKitTM – basal medium and SingleQuotsTM Kit packaged together

CloneticsTM KGMTM-2 角质形成细胞生长培养基-2 已针对在无血清环境中的角质形成细胞增殖进行了优化,通常提供比 KGMTM 培养基更高的生长速率。

CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。 媒体系统已针对各种细胞系统专门开发和优化。 媒体以多种格式提供:

基础培养基 – 不含生长因子细胞因子或补充剂

SingleQuotsTM 试剂盒——生长因子、细胞因子和补充剂

BulletKitTM – 基础培养基和 SingleQuotsTM 套件包装在一起


Get more information on the discontinuation of certain keratinocytes products.

KBM™- 2 Keratinocyte Basal Medium-2

Safety Data Sheets (SDS)

View SDS

SDS – Dutch
SDS – German
SDS – Italian
SDS – Spanish
SDS – French
SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza 4W-270A,原代细胞和干细胞,人外周血单个核细胞 (hPBMC)

Catalog #: 4W-270A
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells

Product Overview

来自白细胞分离术的富含单核细胞 (MNC) 的细胞耗尽了红细胞和血小板。 MNC 池平均含有 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞、10% 的 NK 细胞,并且可能含有高达 5% 的粒细胞。 细胞在 Lonza 冷冻保存培养基中冷冻保存。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。

由 AllCells® 制造

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

  • ELISPOT
  • Proliferation assays
  • Immunogenicity assays
  • Cell therapy process development
  • Humanized mice
  • Biomarker discovery

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells

lonza PT-3927,人牙髓干细胞基础培养基,DPSC-人牙髓干细胞 PT-5025

Catalog #: PT-3927
Human Dental Pulp Stem Cell Basal Medium, 500 mL

Product Overview

DPSC 牙髓干细胞 BulletKitTM 培养基; DPSC 牙髓干细胞 BulletKitTM 培养基以 BulletKitTM 培养基(目录号 PT-3005)的形式提供,其中包括基础培养基和人牙髓衍生间充质干细胞增殖所需的补充剂。 基础培养基(DPSC 基础培养基;目录号 PT-3927)和必要的补充剂(DPSC SingleQuot 试剂盒;目录号 PT-4516)也可以单独购买。

Related Products
Human Dental Pulp Stem Cell Basal Medium
DPSC – Human Dental Pulp Stem Cells PT-5025
DPSC – 人牙髓干细胞 PT-5025

lonza CC-4205,原代细胞和干细胞,人转铁蛋白0.5 mL

Catalog #: CC-4205
Human Transferrin, 0.5 mL

Product Overview

人转铁蛋白是控制生物体液中游离铁水平的铁结合血浆糖蛋白。 人转铁蛋白由 TF 基因编码。 人转铁蛋白糖蛋白与铁的结合非常紧密,但可逆,是最重要的铁池,具有最高的周转率 (25 mg/24 h)。 转铁蛋白的分子量约为 80 KDa,并包含两个特定的高亲和力 Fe(III) 结合位点。 人转铁蛋白的浓度为 10 mg/ml

Content & Storage

Content

1 x Vial Containing 10mg/mL Human Transferrin 0.5 mL

lonza CC-7014,原代细胞和干细胞,AoAF人主动脉外膜成纤维细胞

Catalog #: CC-7014
AoAF, Aortic Adventitial Fibroblasts

Product Overview

人心脏相关成纤维细胞包括从升主动脉或降主动脉外部的被膜分离的主动脉外膜成纤维细胞 (AoAF) 和从心房或心室分离的心脏成纤维细胞 (NHCF-A 和 -V)。 细胞是从正常的成人心脏组织中分离出来的。 冷冻保存的细胞在第 2 代运送。

CloneticsTM NHCF-V 和 -A 通过 5 次群体倍增得到保证,解冻后表达 ≥90% 的胶原蛋白 I 和 ≤10% 的 Von Willibrand 因子 VIII。 CloneticsTM AoAF 通过 10 次群体倍增得到保证,并且对 α 平滑肌肌动蛋白呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Applications

  • Vascular diseases
  • Neointimal formation
  • Vascular remodeling
  • Atherosclerosis
  • Hypertension
  • Myocardial repair
  • Extracellular maintenance during cardiac cell / tissue growth
  • Cardiac Fibrosis Paracrine signalling
  • Toxicology
  • Gene expression

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
AoAF – Human Aortic Adventitial Fibroblasts
NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904

NHCF-V – 人心室心脏成纤维细胞 CC-2904

AoAF – Human Aortic Adventitial Fibroblasts
NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903

NHCF-A – 人心房心脏成纤维细胞 CC-2903

AoAF – Human Aortic Adventitial Fibroblasts
HAEC – Human Aortic Endothelial Cells CC-2535

HAEC – 人主动脉内皮细胞 CC-2535

AoAF – Human Aortic Adventitial Fibroblasts
AoSMC – Human Aortic Smooth Muscle Cells CC-2571

AoSMC – 人主动脉平滑肌细胞 CC-2571

AoAF – Human Aortic Adventitial Fibroblasts
SCGM™ Stromal Cell Growth Medium BulletKit™ CC-3205
SCGM™ 基质细胞生长培养基 BulletKit™ CC-3205

lonza 4C-400,原代细胞和干细胞,人 CD14+ 单核细胞(脐带血)

Catalog #: 4C-400
人 CD14+ 单核细胞,使用阳性免疫磁性选择从脐带血中分离,≥ 500 万个细胞,冷冻保存

Product Overview

来自单个供体的脐带血用于使用免疫磁性 CD14 微珠从单核细胞中阳性选择 CD14+ 单核细胞。 细胞用 Lonza Cryo Media 冷冻保存在冷冻管中。 解冻后使用 FACS 测定法测定纯度。 使用 AO/PI 确定计数和活力。 由 AllCells® 制造

Applications

  • Generate monocyte-derived M1 and M2 macrophages
  • Generate monocyte-derived DCs
  • Dendritic cell differentiation and maturation
  • Toll-like receptor and pattern recognition receptors (PRR)
  • Phagocytosis
  • Metabolism mechanisms

Content & Storage

Content

1 vial containing ≥ 5 million cryopreserved cells

lonza CC-4242,原代细胞和干细胞,神经祖细胞 SingleQuots™ 补充剂

Catalog #: CC-4242
Neural Progenitor Suppl.

Related Products

Neural Progenitor SingleQuots™ Supplements
NHNP – Human Neural Progenitor Cells PT-2599
 NHNP – 人类神经祖细胞 PT-2599

lonza M-CX-400,原代细胞和干细胞,小鼠 CD1 大脑皮层神经元

Catalog #: M-CX-400
Cryopreserved ampule of Mouse CD1 Cortex Neurons containing ≥ 4 million cells

Product Overview

即用型 CloneticsTM 小鼠大脑 CD1 皮层神经元 (M-CX-400) 从小鼠 CD1 大脑 (E14,15) 中分离出来。 这些以标准化方法制备的高质量原代胚胎脑神经元细胞(包括神经胶质细胞)的细胞悬液形式出售,并可立即培养。

每瓶皮层细胞含有大约 400 万个活细胞。 这将使用推荐的电镀密度和培养基播种到 24 孔板的 10 个或更多孔和 96 孔板的 50 个或更多孔中。 培养约 4 天后,细胞将形成神经突网络,到第 7 天,碎片将最少。

推荐介质:PNGMTM BulletKitTM (CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Content & Storage

Content

≥4,000,000 cryopreserved cells per ampule

Related Products
Mouse CD1 Brain Cortex Neurons
Mouse C57 Brain Cortex Neurons M-CX-300

小鼠 C57 大脑皮层神经元 M-CX-300

Mouse CD1 Brain Cortex Neurons
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Mouse CD1 Brain Cortex Neurons
Rat Brain Cortex Neurons R-CX-500

大鼠大脑皮层神经元 R-CX-500

Mouse CD1 Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012
Mouse CD1 Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
Mouse CD1 Brain Cortex Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
Mouse CD1 Brain Cortex Neurons
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096
Mouse CD1 Brain Cortex Neurons
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960
Mouse CD1 Brain Cortex Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Mouse CD1 Brain Cortex Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-3225,原代细胞和干细胞,CDM™ 软骨细胞分化基础培养基

Catalog #: CC-3226
CDMTM Chondrocyte Differentiation Basal Medium

Product Overview

CDMTM 是一种软骨细胞分化基础培养基,单独出售。 这可以与 1 套 CC-4408(软骨细胞分化培养基 SingleQuotsTM 补充剂和生长因子)一起使用。 由基础培养基和补充剂组成的完整 CDMTM BulletKitTM 可以使用目录号 CC-3225 一起购买。

  • CDMTM Chondrocyte Differentiation Medium is specially designed for the differentiation of chondrocytes. The BulletKitTM is conveniently packaged with basal medium and supplements included for the differentiation of chondrocytes.
  • CGMTM Chondrocyte  Growth Medium   is also available and sold separately for expansion of human chondrocytes.

Content & Storage

Content

1 x Bottle Basal Medium 250 mL

Related Products
CDM™ Chondrocyte Differentiation Basal Medium
CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225

CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225

CDM™ Chondrocyte Differentiation Basal Medium
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216

CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

CDM™ Chondrocyte Differentiation Basal Medium
NHAC-kn – Human Knee Articular Chondrocytes CC-2550
 NHAC-kn – 人膝关节软骨细胞 CC-2550

lonza CC-2933,原代细胞和干细胞,D-SAEC-CF患病的小气道上皮细胞-囊性纤维化

Catalog #: CC-2933
D-SAEC-CF, Small Airway Epithelial Cells, Cystic Fibrosis

Product Overview

囊性纤维化是一种会导致在肺部、消化道和身体其他部位积聚粘稠粘液的疾病。 它是儿童和年轻人最常见的慢性肺部疾病之一。 这种疾病是影响美国高加索人的最常见、最致命的遗传性疾病。 疾病最终恶化到该人残疾的程度。 如今,能够活到成年的 CF 患者的平均寿命约为 37 岁,在过去的 30 年中显着增加。

Lonza 的人小气道上皮细胞 (SAEC) 是从囊性纤维化人肺组织中分离出来的。 冷冻保存的 CloneticsTM SAEC 保证通过 15 次群体倍增和细胞角蛋白 19 染色呈阳性。所有细胞的支原体、细菌、酵母和真菌检测均为阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis
SAEC – Small Airway Epithelial Cells for S-ALITM Small Airway Air-Liquid-Interface Medium CC-2547S

SAEC – 用于 S-ALITM 小气道气液界面培养基 CC-2547S 的小气道上皮细胞

D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis
D-SAEC-COPD – Diseased Small Airway Epithelial Cells – COPD CC-2934

D-SAEC-COPD – 患病小气道上皮细胞 – COPD CC-2934

D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis
HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527

HMVEC-L – 人肺微血管内皮细胞 CC-2527

D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis
D-SAEC-As – Diseased Small Airway Epithelial Cells – Asthma CC-2932

D-SAEC-As – 患病的小气道上皮细胞 – 哮喘 CC-2932

D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis
DHLF-IPF – Diseased Human Lung Fibroblasts, Idiopathic Pulmonary Fibrosis CC-7231

DHLF-IPF – 患病的人肺成纤维细胞,特发性肺纤维化 CC-7231

D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ CC-3170
BEGM™ 支气管上皮细胞生长培养基 BulletKit™ CC-3170

lonza 00194850,原代细胞和干细胞,D-BSMC-As患病的人支气管平滑肌细胞-哮喘

Catalog #: 00194850
DBSMC-As, Bronchial Smooth Muscle Cells, Asthma

Product Overview

Human bronchial smooth muscle cells are isolated from the major bronchia of asthmatic donors.

Asthma is an inflammatory disorder of the airways, which causes attacks of wheezing, shortness of breath, chest tightness, and coughing in patients. Degrees of severity are unique to individual patients as are responses to drug treatments. Adhesion of eosinophils to bronchial epithelial cells, and the interaction of airway smooth muscle and sub-epithelial fibroblasts play a critical role in asthma pathology. During an asthma attack bronchiole smooth muscle tissue can constrict and decrease the flow of air in the airways. The amount of air flow can further be decreased by inflammation or excess mucus secretion.

Cryopreserved Diseased Bronchial Smooth Muscle cells stain positive for alpha smooth muscle actin and negative for von Willebrandfactor VIII. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitisC are not detected for all donors and/or cell lots. A Certificate of Analysisis provided for each cell lot purchased.

Benefits

  • Gene expression pathways
  • Phenotypic changes to compounds
  • Transfect diseased cells
  • Co-culture with immune cells
  • Airway remodeling studies
  • Cellular interactions

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012
D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1024

D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032
D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960

D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1002

D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010
P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

lonza CC-2508,PrSC人类前列腺基质细胞,PrEC人前列腺上皮细胞 CC-2555

Catalog #: CC-2508
PrSC, Prostate Stromal Cells

Product Overview

前列腺细胞通过产生在生殖中具有多种功能的液体来提供体内的腺体功能。 Lonza 的人类前列腺细胞可从上皮、平滑肌和基质组织中获得。 每种细胞类型都有优化的生长培养基,以获得最佳培养效果。

前列腺基质细胞 (PrSC) 通过 15 次群体倍增得到保证,波形蛋白染色呈阳性,泛细胞角蛋白染色呈阴性。 冷冻保存的 PrSC 在第三或第四通道运输。

所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Applications

  • Benign prostatic hyperplasia (BPH)
  • Prostitis
  • Prostate cancer
  • Procreation research
  • Drug discovery
  • Male prostate physiology

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
PrSC – Human Prostate Stromal Cells
PrEC – Human Prostate Epithelial Cells CC-2555

PrEC – 人前列腺上皮细胞 CC-2555

PrSC – Human Prostate Stromal Cells
PrSMC – Human Prostate Smooth Muscle Cells CC-2587
PrSMC – 人前列腺平滑肌细胞 CC-2587

lonza CC-2925,D-RPTEC患病的人肾近端小管上皮细胞II 型糖尿病,HRCE人肾皮质上皮细胞 CC-2554

Catalog #: CC-2925

D-RPTEC, Renal Proximal Tubule Epithelial Cells, Diabetes T2

Product Overview

Lonza 的人肾细胞包括从对每种细胞类型特异的各种正常人肾组织中分离出来的细胞。 人肾近端小管细胞从近端小管中分离出来,是药物转运蛋白研究的理想选择。

肾上皮细胞表现出极化形态,这对其功能至关重要。 它们重新吸收肾小球滤液中几乎所有的葡萄糖和氨基酸,同时允许其他没有营养价值的物质从尿液中排出。 渗透调节和排泄等生理过程可以在体外用 HRE 进行研究。 人肾上皮细胞也能够内化细菌,并且位于各种先天性、代谢和炎症性疾病的损伤部位附近。

Select References with Lonza’s Renal Cells and Media

  • Diabetic Nephropathy is a chronic, progressive renal disease/diabetic kidney disease that develops in approximately one third of people with diabetes.  In a study, Lonza’s human proximal tubular epithelial cells were cultured in respective media (REGMTM). The group investigated the role of KLK1 (kallikrein) in tubular pro-inflammatory responses in cultured human RPTEC and examined the role of PAR-4 (Protease-Activated Receptor 4) activation in KLK1-mediated signaling in the development of DN. Yiu WH, Wong DW, Chan LY, Leung JC, Chan KW, Lan HY, Lai KN, Tang SC. Tissue Kallikrein Mediates Pro-Inflammatory Pathways and Activation of Protease-Activated Receptor-4 in Proximal Tubular Epithelial Cells. PLoS ONE (2014); 9(2): e88894
  • In another study, the role of micro RNA 1207-5P in the implication of Diabetic nephropathy was shown, where Lonza’s Mesangial cells and normal human renal proximal tubule epithelial cells were used. M. Lucrecia Alvarez , Mahdieh Khosroheidari, Elena Eddy, Jeff Kiefer. Role of MicroRNA 1207-5P and Its Host Gene, the Long Non-Coding RNA Pvt1, as Mediators of Extracellular Matrix Accumulation in the Kidney: Implications for Diabetic Nephropathy. PLoS ONE (2013); 8(10): e77468

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
D-RPTEC – Diseased Human Renal Proximal Tubule Epithelial Cells – Diabetes Type II
RPTEC – Human Renal Proximal Tubule Epithelial Cells CC-2553

RPTEC – 人肾近端小管上皮细胞 CC-2553

D-RPTEC – Diseased Human Renal Proximal Tubule Epithelial Cells – Diabetes Type II
HRCE – Human Renal Cortical Epithelial Cells CC-2554

HRCE – 人肾皮质上皮细胞 CC-2554

D-RPTEC – Diseased Human Renal Proximal Tubule Epithelial Cells – Diabetes Type II
HRE – Human Renal Epithelial Cells CC-2556

HRE – 人肾上皮细胞 CC-2556

D-RPTEC – Diseased Human Renal Proximal Tubule Epithelial Cells – Diabetes Type II
REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190
REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

lonza CC-3112,KGM™ 角质形成细胞生长培养基 BulletKit™无钙

Catalog #: CC-3112
KGMTM Keratinocyte BulletKitTM, Calcium Free

Product Overview

KGMTM 角质形成细胞生长培养基已针对角质形成细胞在无血清和无钙环境中的增殖进行了优化。 角质形成细胞不能在完全不含钙的系统中存活。 有关角质形成细胞钙需求的信息,请联系科学支持。

您知道我们的客户也使用 KGMTM 培养基培养永生化角膜上皮细胞吗? 看看 Jolly 等。 人。 提供了一些指导方针的出版物。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL

lonza 4W-400,原代细胞和干细胞,人外周血 CD14+ 单核细胞

Catalog #: 4W-400
Cryopreserved ampule of Human Peripheral Blood CD14+ Monocytes, negative selection, containing ≥10 million cells

Product Overview

白细胞分离的 CD14+ 单核细胞是从白细胞分离的外周血 MNC 中阴性选择的,使用免疫磁性标记系统去除不需要的细胞类型,使单核细胞不受影响。 细胞在冷冻管中的 Lonza Cryo 培养基中冷冻保存。 解冻后使用 FACS 测定确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。

由 AllCells® 制造

Benefits

  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

  • Generate monocyte-derived M1 and M2 macrophages
  • Generate monocyte-derived DCs
  • Dendritic cell differentiation and maturation
  • Toll-like receptor and pattern recognition receptors (PRR)
  • Phagocytosis
  • Metabolism mechanisms

Content & Storage

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 10 million cells

Related Products
Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Negative Selection, 10 million
X-VIVOTM 15 Serum-free Hematopoietic Cell Medium BE02-060Q

X-VIVOTM 15 无血清造血细胞培养基 BE02-060Q

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Negative Selection, 10 million
X-VIVOTM 15 Serum-free Hematopoietic Cell Medium BE02-053Q

X-VIVOTM 15 无血清造血细胞培养基 BE02-053Q

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Negative Selection, 10 million
X-VIVOTM 20 Serum-free Hematopoietic Cell Medium BE04-448Q
X-VIVOTM 20 无血清造血细胞培养基 BE04-448Q

lonza PT-4122,hMSC 人间充质干细胞成脂维持培养基 SingleQuotsTM 添加剂和生长因子

Catalog #: PT-4122
hMSC Adipogenic Maintenance SingleQuotsTM Kit
hMSC 脂肪生成维护 SingleQuotsTM 试剂盒
lonza PT-4122,hMSC 人间充质干细胞成脂维持培养基 SingleQuotsTM 添加剂和生长因子

lonza CC-2545,原代细胞和干细胞,HIAEC-人髂动脉内皮细胞

Catalog #: CC-2545
HIAEC, Iliac Artery Endothelial Cells

Product Overview

原代人髂动脉 (HIAEC) 内皮细胞是从正常组织中分离出来的。 其他供体特征,包括例如 可能有心脏病、高血压或吸烟史。 如需更多信息,请联系我们的科学支持团队。

CloneticsTM 细胞、培养基和试剂一起经过质量测试,保证作为一个完整的细胞系统提供最佳性能。 冷冻保存的 HIAEC 在第三通道中运输。

冷冻保存的 HIAEC 可通过 10 次人口倍增得到保证。 HIAEC 表达 CD31/105、von Williebrand Factor VIII,并且对乙酰化低密度脂蛋白摄取呈阳性。 所有细胞的支原体、细菌、酵母和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

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HIAEC – Human Iliac Artery Endothelial Cells
HCAEC – Human Coronary Artery Endothelial Cells CC-2585

HCAEC – 人冠状动脉内皮细胞 CC-2585

HIAEC – Human Iliac Artery Endothelial Cells
HPAEC – Human Pulmonary Artery Endothelial Cells CC-2530
HPAEC – 人肺动脉内皮细胞 CC-2530
HIAEC – Human Iliac Artery Endothelial Cells
EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM CC-3202

EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM CC-3202

HIAEC – Human Iliac Artery Endothelial Cells
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012

P5 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-5012

HIAEC – Human Iliac Artery Endothelial Cells
P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024
HIAEC – Human Iliac Artery Endothelial Cells
P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032

P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032

HIAEC – Human Iliac Artery Endothelial Cells
P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096

P5 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-5096

HIAEC – Human Iliac Artery Endothelial Cells
P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960

P5 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-5960

HIAEC – Human Iliac Artery Endothelial Cells
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002
HIAEC – Human Iliac Artery Endothelial Cells
P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010
P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5010

lonza CC-2926,D-HEK-Ad患病的人表皮角质形成细胞,成人II 型糖尿病

Catalog #: CC-2926

D-HEK-Ad, Epidermal Keratinocytes, Adult, Diabetes T2

Product Overview

患病的人类表皮角质形成细胞 (D-HEK) 是从被诊断患有 II 型糖尿病的人类供体中分离出来的。

可以通过联系 Lonza Scientific Support 获得更多的捐赠者信息,包括药物、BMI 和疾病持续时间。 患病细胞在与正常人角质形成细胞相同的优化培养基系统中生长。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products
D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II
NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627

NHEK-Ad – 人表皮角质形成细胞,成人,单一供体 00192627

D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II
HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543

HMVEC-dAd – 人真皮微血管内皮细胞 – 成人 CC-2543

D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II
NHDF-Ad – Human Dermal Fibroblasts, Adult CC-2511

NHDF-Ad – 人皮肤成纤维细胞,成人 CC-2511

D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II
NHEM-Ad – Human Epidermal Melanocytes, Adult CC-2586
NHEM-Ad – 人表皮黑色素细胞,成人 CC-2586

lonza 2M-101A,原代细胞和干细胞,人骨髓 CD34+ 祖细胞

Catalog #: 2M-101A

来自骨髓的人 CD34+ 祖细胞的冷冻保存安瓿,含有 ≥ 300,000 个细胞。 使用阳性免疫磁性选择从单核细胞中分离细胞。

Product Overview

骨髓 CD34+ 细胞含有造血干细胞和祖细胞,并且已知可分化成所有不同的血细胞类型。

Lonza骨髓CD34+细胞通过阳性免疫磁分离从骨髓单个核细胞(MNC)中分离出来,保证纯度≥90%。根据订购的产品,它们保证每个安瓿含有≥100,000、≥300,000、≥500,000、≥100 万或 ≥200 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、丙型肝炎和乙型肝炎。为购买的每个细胞批次提供分析证书。提供各种大小的新鲜骨髓 CD34+ 细胞,每个供体至少需要两瓶。有关新鲜细胞的尺寸和价格信息,请联系客户服务。

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能(根据应用可能需要额外的细胞因子) 也可以接受含有血清的替代培养基。有关含血清培养基的建议,请联系科学支持。

复杂的研究通常需要灵活性。这就是为什么我们提供分离和纯化的骨髓 CD34+ 细胞以及未加工的骨髓,以防您需要分离自己的细胞。骨髓 CD34+ 细胞非常适用于需要来自单个供体的大量细胞的研究或调查不同年龄、性别或种族供体的细胞差异的研究。但是,如果您的研究需要更多幼稚细胞,脐带血 CD34+ 细胞可能是更好的选择。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

Benefits

  • Ready to use
  • Wide donor variety
  • Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34+ cells)

Content & Storage

Content

1 x Cryopreserved ampule of Human Bone Marrow CD34+ containing ≥ 300,000 cells

Related Products
Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 300,000
Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞,500 万个 2M-302

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 300,000
Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

未加工的人骨髓,新鲜,10 mL 1M-105

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 300,000
MycoAlert® Mycoplasma Detection Kit (25 Tests) LT07-218
MycoAlert® 支原体检测试剂盒(25 次测试)LT07-218

lonza MBCS01,原代细胞和干细胞,小鼠 C57BL/6 肝细胞

Catalog #: MBCS01

Cryopreserved ampule of C57BL/6 Mouse Hepatocytes, in suspension format, containing > 5 million cells

Product Overview

我们所有的悬浮冷冻保存的原代动物肝细胞都经过专门预测试,以了解悬浮液中一般 I 期和 II 期代谢的代谢活性。 它们不适用于需要贴壁细胞的应用。

Benefits

  •  Certificate of Analysis (CoA) provided for every cell lot

Applications

  • Drug metabolism in suspension format
  • Species-species comparisons
  • Genomics

Content & Storage

Content

Cryo vial, > 5 million viable cells

Related Products
Mouse C57BL/6 Hepatocytes, Cryopreserved, Suspension
Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞,冷冻保存,可电镀 HUCPG

Mouse C57BL/6 Hepatocytes, Cryopreserved, Suspension
Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI
人肝细胞,冷冻保存,可电镀和相互作用合格的 HUCPI

lonza PT-4516,原代细胞和干细胞,人牙髓干细胞 SingleQuots™ 补充剂和生长因子

Catalog #: PT-4516
Human Dental Pulp Stem Cell SingleQuotsTM Kit

Product Overview

DPSC 牙髓干细胞 BulletKitTM 培养基; DPSC 牙髓干细胞 BulletKitTM 培养基以 BulletKitTM 培养基(目录号 PT-3005)的形式提供,其中包括基础培养基和人牙髓衍生间充质干细胞增殖所需的补充剂。 基础培养基(DPSC 基础培养基;目录号 PT-3927)和必要的补充剂(DPSC SingleQuot 试剂盒;目录号 PT-4516)也可以单独购买。

Related Products

Human Dental Pulp Stem Cell SingleQuots™ Supplements and Growth Factors
DPSC – Human Dental Pulp Stem Cells PT-5025
DPSC – 人牙髓干细胞 PT-5025

lonza M-CP-302,小鼠 C57 脑纹状体神经元,PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Catalog #: M-CP-302
Cryopreserved ampule of Mouse C57 Striatum Neurons containing ≥ 4 million cells

Product Overview

即用型 CloneticsTM 小鼠 C57 脑纹状体神经元 (M-CP-302) 分离自小鼠 C57 黑脑 (E14, 15)。 这些以标准化方法制备的高质量原代胚胎脑神经元细胞(包括神经胶质细胞)的细胞悬液形式出售,并可立即培养。

每瓶纹状体细胞含有大约 400 万个活细胞。 这将使用推荐的电镀密度和培养基播种到 24 孔板的 6 个或更多孔和 96 孔板的 30 个或更多孔中。 培养约 4 天后,细胞将形成神经突网络,到第 7 天,碎片将最少。

推荐介质:PNGMTM BulletKitTM (CC-4461)

Benefits

  • Ready-To-Use
  • No tissue dissection or animal handling required
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch

Applications

  • Transfection
  • Electrophysiological properties
  • Neurotransmitter function
  • Inhibitory / excitatory ion-channels
  • Receptor signaling
  • Intracellular transport studies
  • Neurotoxicity screening

Content & Storage

Content

≥ 4,000,000 cryopreserved cells per ampule

Related Products
Mouse C57 Brain Striatum Neurons
PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Mouse C57 Brain Striatum Neurons
Mouse CD1 Brain Striatum Neurons M-CP-402
Mouse C57 Brain Striatum Neurons
Rat Brain Striatum Neurons R-CP-502
Mouse C57 Brain Striatum Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012

Mouse C57 Brain Striatum Neurons
P3 Primary Cell 4D-Nucleofector® X Kit L V4XP-3024
Mouse C57 Brain Striatum Neurons
P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032
Mouse C57 Brain Striatum Neurons
P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

Mouse C57 Brain Striatum Neurons
P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

Mouse C57 Brain Striatum Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

Mouse C57 Brain Striatum Neurons
P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010
P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-4202,原代细胞和干细胞,氯化钙

Catalog #: CC-4202
Calcium Chloride, 300 mM, 2 mL

Product Overview

Calcium Chloride is available at a concentration of 300mM solution.

氯化钙的浓度为 300mM 溶液。

This is included for use in select primary cell culture media kits

Content & Storage

Content

1 Vial Containing 2 mL 300 mM Calcium Chloride

lonza 00194843,原代细胞和干细胞,DHLF-CF 患病的人肺成纤维细胞-囊性纤维化

Catalog #: 00194843
DHLF-CF, Lung Fibroblasts, Cystic Fibrosis
lonza 00194843,原代细胞和干细胞,DHLF-CF 患病的人肺成纤维细胞-囊性纤维化

Product Overview

从囊性纤维化供体的成人肺组织中分离出患病的成纤维细胞。肺间质中最丰富的细胞类型是成纤维细胞。它们类似于普通的成纤维细胞,但具有一些显着特征,例如,它们具有长分支过程和间隙连接。它们的主要功能是产生肺泡隔细胞外基质的 III 型胶原蛋白、弹性蛋白和蛋白聚糖。

肺成纤维细胞 (PF) 在损伤后的修复和重塑过程中发挥重要作用。成纤维细胞在炎症部位的受控积累对于损伤后的有效组织修复至关重要。成纤维细胞的不充分或过度积累都可能导致组织功能异常。例如,成纤维细胞的过度增殖导致在缺氧诱导的肺动脉高压发展过程中观察到的外膜增厚。

来自囊性纤维化供体的冷冻保存的患病肺成纤维细胞对血管性血友病因子 VIII、细胞角蛋白 18 和 19 以及 α 平滑肌肌动蛋白染色呈阴性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

联系科学支持以获取详细的捐赠者信息,包括诊断年龄、服用的药物和吸烟史。

Benefits

Applications:

  • Gene expression pathways
  • Phenotypic changes to compounds
  • Transfect diseased cells
  • Co-culture with immune cells
  • Airway remodeling studies
  • Cellular interactions

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

lonza PT-5007,原代细胞和干细胞,糖尿病 I 型和 II 型脂肪干细胞 (ADSC)

Catalog #: PT-5007
Human ADSC, cryo amp, ≥1 million cells, Diabetes Type I

Product Overview

糖尿病是一种慢性(终生)疾病,其特点是血液中的糖分含量很高。食用食物时,葡萄糖会进入血液。葡萄糖是身体的燃料来源。胰腺产生胰岛素,将葡萄糖从血液中转移到肌肉、脂肪和肝细胞中,在那里它可以用作燃料。糖尿病患者血糖高,因为胰腺不能产生足够的胰岛素,或者他们的肌肉、脂肪和肝细胞对胰岛素没有正常反应,或者两者兼而有之。

在 I 型糖尿病中,身体产生很少或不产生胰岛素。需要每天注射胰岛素。确切原因尚不清楚;遗传、病毒和自身免疫问题可能起作用。

在 II 型糖尿病中,胰腺不能产生足够的胰岛素来保持血糖水平正常,这通常是因为身体对胰岛素的反应不佳。

PoieticsTM 糖尿病人脂肪源性干细胞 (ADSC) 是从选择性手术吸脂手术期间从 I 型糖尿病和 II 型糖尿病供体收集的成人脂肪抽吸物中分离出来的。来自正常(非糖尿病)供体的人体脂肪干细胞 (ADSC) 作为单独的产品提供。这些细胞表现出与骨髓来源的间充质干细胞非常相似的表型和功能特征。据报道,糖尿病人脂肪源性干细胞 (ADSC) 可分化出许多不同的谱系,包括软骨形成、成骨、脂肪形成和神经细胞。糖尿病人脂肪源性干细胞 (ADSC) 已在初级传代时冷冻保存。联系 ScientificSupport 可获取相关的额外供体信息,包括体重指数 (BMI)、胰岛素依赖和诊断年龄。

冷冻保存的 PoieticsTM 糖尿病人脂肪衍生干细胞 (ADSC) 保证通过 5 次传代,表达 CD13、CD29、CD44、CD73、CD90、CD105 和 CD166,并且不表达 CD14、CD31 和 CD45。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

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Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC)
ADSC Growth Medium BulletKit™ PT-4505
ADSC 生长培养基 BulletKit™ PT-4505

lonza CC-4409,CGM™ 软骨细胞生长培养基 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4409
CGMTM Chondrocyte Growth SingleQuotsTM Kit

Product Overview

CGMTM SingleQuotTM 试剂盒是单独出售的组件,不包括基础培养基。 这些是 CGMTM 软骨细胞生长培养基 BulletKitTM(目录号 CC-3216)的一部分。 由基础培养基和补充剂组成的完整 CGMTM BulletKitTM 可以使用目录号 CC-3216 一起购买。

  • CGMTM Chondrocyte Growth Medium is specially designed for expansion of chondrocytes. The BulletKitTM is conveniently packaged with basal medium and supplements included for the growth of chondrocytes.
  • CDMTM Chondrocyte Differentiation Medium is also available and sold separately for differentiation of human chondrocytes

Content & Storage

Content

1 x Bottle FBS 25.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Yellow Cap Vial with R3-IGF 1.00 mL
1 x Bottle bFGF 2.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Lilac Cap Vial with Insulin 1.00 mL

Related Products
CGM™ Chondrocyte Growth Medium SingleQuots™ Supplements and Growth Factors
NHAC-kn – Human Knee Articular Chondrocytes CC-2550

NHAC-kn – 人膝关节软骨细胞 CC-2550

CGM™ Chondrocyte Growth Medium SingleQuots™ Supplements and Growth Factors
CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225

CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225

CGM™ Chondrocyte Growth Medium SingleQuots™ Supplements and Growth Factors
CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216
 CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

lonza CC-4134,FGM™ 成纤维细胞生长培养基 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4134

停产通知:根据我们为在我们的产品组合中保持高服务水平而进行的产品生命周期管理审查,该产品将在现有库存耗尽后停产。 我们建议 CC-3245 作为 CC-3132 的替代品。

FGMTM SingleQuotsTM 试剂盒、人成纤维细胞培养所需的补充剂和生长因子(基础培养基单独出售)

Product Overview

FGMTM SingleQuotTM 试剂盒是单独出售的组件,不包括基础培养基。 这些是 FGMTM 成纤维细胞生长培养基 BulletKitTM(目录号 CC-3130)的一部分,它由包装在一起的基础培养基和补充剂组成。

lonza 4W-601,原代细胞和干细胞,人 CD19+ B 细胞,人 CD19+ B 细胞4W-600

Catalog #: 4W-601
使用负免疫磁性选择从外周血中分离出人 CD19+ B 细胞,冷冻保存,≥1000 万个细胞

Product Overview

白细胞分离的 CD19+ B 细胞是从白细胞分离的外周血 MNC 中阴性选择的,使用免疫磁性标记系统去除不需要的细胞类型,使 B 细胞不受影响。 细胞在冷冻管中的 Lonza Cryo 培养基中冷冻保存。 解冻后使用 FACS 测定确定细胞纯度。 使用 AO/PI 确定计数和活力。 按照 IRB 协议从健康供体收集细胞。

Applications

  • Generating antibody libraries
  • Co-culture with T helper cells or cytotoxic lymphocytes (CTL)
  • B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
  • Antigen presenting cell (APC) signal transduction and antigen presentation

Content & Storage

Content

1 vial containing ≥ 10 million cryopreserved cells

Related Products

Human CD19+ B Cells (10 Million Cells, Negative Selection)
Human CD19+ B Cells (10 Million Cells, Positive Selection) 4W-600
人 CD19+ B 细胞(1000 万细胞,正选择)4W-600

lonza 00195407,RtEGM™ 视网膜上皮细胞生长培养基 SingleQuots™ 添加剂和生长因子

Catalog #: 00195407

视网膜上皮生长培养基 (RtEGM) SingleQuotsTM 补充剂和生长因子

Product Overview

RtGMTM SingleQuotTM 试剂盒是单独出售的组件,不包括基础培养基。 这些是 RtEGMTM 视网膜上皮细胞生长培养基 BulletKitTM(目录号 195409)的一部分。 可以使用目录号 195409 一起购买由基础培养基和补充剂组成的完整 RtEGMTM BulletKitTM。

RtEGMTMTM 视网膜色素上皮细胞生长培养基针对生长的人类 RPE 细胞进行了优化和性能测试。 它含有2%的血清。

Benefits

 Applications:

  • Age-related macular degeneration
  • Retinitis pigmentosa
  • Diabetic retinopathy
  • Photoreceptor degeneration
  • Gene expression
  • Cellular interactions

Content & Storage

Content

1 x Bottle with L-Glutamine 4.00 mL, 1 x Gray Cap Vial with hFGF-B 1.00 mL, 1 x Bottle FBS 4.00 mL, 1 x Red Cap Vial with GA-1000 0.20 mL

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RtEGM™ Retinal Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors
RtEBM™ Retinal Epithelial Cell Basal Medium 00195406
RtEBM™ 视网膜上皮细胞基础培养基 00195406
RtEGM™ Retinal Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors
H-RPE – Human Retinal Pigment Epithelial Cells 00194987

H-RPE – 人视网膜色素上皮细胞 00194987

RtEGM™ Retinal Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors
RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™ 00195409
 RtEGM™ 视网膜色素上皮细胞生长培养基 BulletKit™ 00195409

lonza PT-5022,原代细胞和干细胞,患病的人类皮下前脂肪细胞-II 型糖尿病

Catalog #: PT-5022
Human Subcutan. Preadipocyte Cells, Diabetes Type II

Product Overview

PoieticsTM 患病人类前脂肪细胞 (D-HprAD) 是从 I 型糖尿病和 II 型糖尿病供体的皮下或内脏脂肪中分离出来的。来自正常(非糖尿病)供体的前脂肪细胞可作为单独的产品获得。相关的额外供体信息,包括体重指数 (BMI)、胰岛素依赖和诊断年龄,可通过联系科学支持获得。冷冻保存的 PoieticsTM 患病人类前脂肪细胞 (D-HprAD) 保证每安瓿含有 ≥ 100 万个细胞,并且脂肪分化检测呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。推荐培养基:PGMTM -2 Preadipocyte Growth Medium-2 BulletKitTM 培养基(提供增殖培养基和分化培养基,保证诱导分化为成熟、功能活跃的脂肪细胞;每瓶 PGM-2TM BulletKitTM 培养基都含有足够的分化培养基以完全分化1000 万个前脂肪细胞 [10 个完整的 96 孔板] 变成成熟的、功能活跃的脂肪细胞)。 PGMTM-2 培养基以 BulletKitTM 培养基(目录号 PT-8002)的形式提供,其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。基础培养基(PGMTM-2 基础培养基;目录号 PT-8202)和必要的补充剂(PGMTM-2 SingleQuotTM 试剂盒;目录号 PT-9502)也可以单独购买。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时,Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改,包括使用替代培养基、试剂或方案,都将使细胞和培养基性能保证失效。

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PBM-2 前脂肪细胞基础培养基-2 PT-8202

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002
Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010
P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

lonza CC-2587,原代细胞和干细胞,PrSMC-人前列腺平滑肌细胞

Catalog #: CC-2587
PrSMC, Prostate Smooth Muscle Cells

Product Overview

前列腺细胞通过产生在生殖中具有多种功能的液体来提供体内的腺体功能。 Lonza 的人类前列腺细胞可从上皮、平滑肌和基质组织中获得。 每种细胞类型都有优化的生长培养基,以获得最佳培养效果。

前列腺平滑肌细胞 (PrSMC) 通过 10 次群体倍增得到保证,α 平滑肌肌动蛋白染色呈阳性,von Willebrand(因子 VIII)抗原染色呈阴性。 冷冻保存的 PrSMC 在第二次或第三次运输中运输。

所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。

Applications

  • Benign prostatic hyperplasia (BPH)
  • Prostitis
  • Prostate cancer
  • Procreation research
  • Drug discovery
  • Male prostate physiology

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

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PrSMC – Human Prostate Smooth Muscle Cells
PrSC – Human Prostate Stromal Cells CC-2508

PrSC – 人类前列腺基质细胞 CC-2508

PrSMC – Human Prostate Smooth Muscle Cells
PrEC – Human Prostate Epithelial Cells CC-2555

PrEC – 人前列腺上皮细胞 CC-2555

PrSMC – Human Prostate Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012
PrSMC – Human Prostate Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024
PrSMC – Human Prostate Smooth Muscle Cells
P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032
PrSMC – Human Prostate Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

PrSMC – Human Prostate Smooth Muscle Cells
P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960
PrSMC – Human Prostate Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1002

PrSMC – Human Prostate Smooth Muscle Cells
P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010
P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010