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lonza CC-2551,原代细胞和干细胞,HMEC-人乳腺上皮细胞

Catalog #: CC-2551

Cryopreserved ampule of Human Mammary Epithelial Cells (HMEC) containing ≥500,000 cells

Product Overview

Lonza’s Human Mammary Epithelial Cells (HMEC) are isolated from adult female breast tissue. The cells have been primarily utilized to understand the formation of breast cancer. Cryopreserved Human Mammary Epithelial Cells are guaranteed through 15 population doublings, express cytokeratins 14 and 18 and are negative for cytokeratin 19.

Lonza 的人乳腺上皮细胞 (HMEC) 是从成年女性乳腺组织中分离出来的。这些细胞主要用于了解乳腺癌的形成。冷冻保存的人乳腺上皮细胞通过 15 次群体倍增，表达细胞角蛋白 14 和 18，对细胞角蛋白 19 呈阴性。

Select publications highlighting use of HMECs:

In an article, Xinyi Guo et al 2014 New J. Phys. 16 105002, the research team utilized Lonza’s HMEC side-by-side with immortalized HMEC and well-established breast cancer cell lines MCF7 and MDA-MB-231 to understand the effect of neighboring cells on the stiffness of cancerous and non-cancerous human mammary epithelial cells.
In the article, Elenbaas et al. Genes & Development. 2001, HMECs were propagated in MEGM^TM BulletKit^TM. HMECs expressing LT and hTERT were infected with a retrovirus encoding H-rasV12 and were introduced in mice. The resulting data showed that HMECs have successfully been transformed to tumorigenicity through the introduction of a limited set of oncogenes.

Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGM^TM BulletKit^TM Growth Media (catalog no. CC-3150)

Benefits

Guaranteed through 15 population doublings
Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of HMEC containing ≥500,000 cells

Related Products

PrEC – Human Prostate Epithelial Cells CC-2555

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ CC-3150

P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 Primary Cell 4D-Nucleofector^® X Kit L V4XP-3024

P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

HMEC – Human Mammary Epithelial Cells

Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems

Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGM™ BulletKit™ Medium vs. other commercially available systems

lonza,lonza PT-3004,人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM

Catalog #: PT-3004

Culture system containing basal medium and supplements required for maintenance and induction of hMSC adipogenic differentiation

Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including adipocytes. Lonza’s hMSC Adipogenic Differentiation BulletKit^TM Medium is a serum containing media system designed to induce adipogenic differentiation of bone marrow derived hMSC into mature, functionally active adipocytes in as few as 19 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s AdipoRed^TM Adipogenic Assay Reagent which provides a quantitative value related to the amount of adipogenic differentiation.

干细胞具有分化成包括脂肪细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 成脂分化 BulletKitTM 培养基是一种含血清的培养基系统，旨在在短短 19 天内诱导骨髓来源的 hMSC 成脂分化成成熟的、功能活跃的脂肪细胞。然后可以通过多种方法验证分化，包括使用 Lonza 的 AdipoRedTM 脂肪生成分析试剂，该试剂提供与脂肪生成分化量相关的定量值。

Partial List of Cells Differentiated with hMSC Adipogenic Differentiation BulletKit^TM Medium*:

Human bone marrow derived stem cells
C57BL/6 mice bone marrow derived stem cells
human adipose derived stem cells
Human periodontal ligament derived stem cells
Human hamstring tendon derived stem cells
hMSC 成脂分化 BulletKitTM 培养基提供诱导培养基和维持培养基，保证诱导骨髓来源的 hMSC 成脂分化成成熟的、功能活跃的脂肪细胞；每瓶 hMSC 脂肪分化 BulletKitTM 培养基都含有足够的诱导培养基和维持培养基，可以将大约 300 万个骨髓来源的 hMSC [大约 15 个孔，以 6 孔板形式] 完全分化成成熟的功能活跃的脂肪细胞。hMSC 脂肪分化培养基以 BulletKitTM 培养基（目录号 PT-3004）的形式提供，其中包括用于诱导和维持的基础培养基以及用于脂肪形成诱导和维持骨髓来源的 hMSCs 的必要补充剂。只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。
*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。

Content & Storage

Content

1 x hMSC Adipogenic Maintenance Medium (PT-3102A)

1 x hMSC Adipogenic Induction Medium (PT-3102B)

1 x hMSC Adipogenic Maintenance SingelQuot Kit (PT-4122)

1 x hMSC Adipogenic Induction SingleQuot Kit (PT-4135)

Related Products

Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKitTM

Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKit^TM PT-3002

人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002

Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKit^TM PT-3003

人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM PT-3003

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124

用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3

hADSC Human Adipose-Derived Stem Cells PT-5006

hADSC 人体脂肪干细胞 PT-5006

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007

糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5007

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008

糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5008

DPSC – Human Dental Pulp Stem Cells PT-5025

DPSC – 人牙髓干细胞 PT-5025

HprAD – Human Subcutaneous Preadipocyte Cells PT-5001

HprAD – 人皮下前脂肪细胞 PT-5001

Human Visceral Preadipocyte Cells PT-5005

人内脏前脂肪细胞 PT-5005

PGM-2^TM Preadipocyte Growth Medium-2 BulletKit^TM PT-8002

PGM-2TM 前脂肪细胞生长培养基-2 BulletKitTM PT-8002

lonza,CC-7231,DHLF-IPF-患病的人肺成纤维细胞特发性肺纤维化

Catalog #: CC-7231

冷冻保存的患病人肺成纤维细胞、特发性肺纤维化 (DHLF-IPF) 安瓿，含有 ≥ 500,000 个细胞

Product Overview

IPF (Idiopathic Pulmonary Fibrosis) is a fatal disease that usually affects adults between the ages of 50-70. The disease is characterized by progressive decline in lung function resulting from scarring of lung tissue.

IPF varies from person to person where in some, fibrosis happens quickly, while the process can be much slower in others. IPF has no cure yet and both geneticand environmental factors are attributed to the development of the disease. Genetics may play a role in causing IPF, and cigarette smoking is the most accepted risk factor in increasing the chances of developing IPF. Recent research has helped doctors understand the disease more closely so they can more quickly diagnose it than in the past. Efforts are still underway in the research community to understand the pathways of disease progression and subsequently develop cures for IPF. IPF was once thought to be a chronic inflammatory process but recent evidence suggests that the abnormal activation of alveolar epithelial cells (AECs) induce over-activation of fibrotic response. The fibroblast and myofibroblast foci secrete excessive amounts of collagens, leading to scarringand destruction of the lung architecture. The mechanisms that link IPF with ageing and aberrant epithelial activation are unknown.

To understand the progression of IPF disease, Lonza offers cryopreserved lung fibroblasts isolated from donors diagnosed with IPF. Cryopreserved IPF fibroblasts are shipped at passage 2 and stain negative for von Willebrand factor VIII, cytokeratins 14, 18 and 19, and alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

IPF（特发性肺纤维化）是一种致命疾病，通常影响 50-70 岁的成年人。该疾病的特征是肺组织瘢痕形成导致肺功能进行性下降。

IPF 因人而异，在某些人中，纤维化发生得很快，而在另一些人中该过程可能要慢得多。 IPF 还没有治愈方法，遗传和环境因素都归因于疾病的发展。遗传可能在导致 IPF 中起作用，而吸烟是增加 IPF 发生机会的最公认的风险因素。最近的研究帮助医生更深入地了解这种疾病，因此他们可以比过去更快地诊断它。研究界仍在努力了解疾病进展的途径并随后开发 IPF 的治疗方法。 IPF 曾被认为是一种慢性炎症过程，但最近的证据表明，肺泡上皮细胞 (AEC) 的异常激活会导致纤维化反应的过度激活。成纤维细胞和肌成纤维细胞病灶分泌过量的胶原蛋白，导致肺结构的瘢痕形成和破坏。将 IPF 与衰老和异常上皮激活联系起来的机制尚不清楚。

为了了解 IPF 疾病的进展，Lonza 提供了从诊断为 IPF 的供体中分离出来的冷冻保存的肺成纤维细胞。冷冻保存的 IPF 成纤维细胞在第 2 代运输，并且对血管性血友病因子 VIII、细胞角蛋白 14、18 和 19 以及 α 平滑肌肌动蛋白染色呈阴性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Relevant Downloads (Lonza’s Normal Human Lung Fibroblasts for IPF Research):

Lung Epithelium Overexpressed Noncoding RNA (LEON): Role of a Novel Long Intergenic Noncoding RNA in Idiopathic Pulmonary Fibrosis
The IL-33/ST2 Axis Is Upregulated in Fibrotic Lung Disease
Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) promotes lung fibroblast proliferation, survival and differentiation to myofibroblasts

Benefits

Gene expression pathways
Phenotypic changes to compounds
Transfect diseased cells
Co-culture with immune cells
Airway remodeling studies
Cellular interaction

Content & Storage

Content

1 x Cryopreserved ampule of DHLF-IPF containing ≥500,000 cells

Related Products

DHLF-IPF - Diseased Human Lung Fibroblasts, Idiopathic Pulmonary Fibrosis

NHLF – Human Lung Fibroblasts CC-2512

SAEC – Human Small Airway Epithelial Cells CC-2547

SAEC – Small Airway Epithelial Cells for S-ALI^TM Small Airway Air-Liquid-Interface Medium CC-2547S

SAEC – 用于 S-ALITM 小气道气液界面培养基 CC-2547S 的小气道上皮细胞

HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527

D-HLF-As – Diseased Human Lung Fibroblast Cells – Asthma 00194912

D-HLF-As – 患病的人肺成纤维细胞 – 哮喘 00194912

D-HLF-COPD – Diseased Human Lung Fibroblast Cell – COPD 00195277

D-HLF-COPD – 患病的人肺成纤维细胞 – COPD 00195277

DHLF-CF-Diseased Human Lung Fibroblasts – Cystic Fibrosis 00194843

DHLF-CF-患病人肺成纤维细胞 – 囊性纤维化 00194843

lonza,PT-3003,人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM

Culture system containing hMSC Chondrogenic Basal Medium (PT-3925) and hMSC Chondrogenic SingleQuots^TM Kit Supplement (PT-4121) required for hMSC chondrogenic differentiation

lonza,PT-3003,人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM

Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including chondrocytes. Lonza’s hMSC Chondrogenic Differentiation BulletKit^TM Medium is a serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin.

干细胞具有分化成包括软骨细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 软骨分化 BulletKitTM 培养基是一种含有血清的培养基，旨在通过添加 TGF-β3 诱导人骨髓来源的间充质干细胞在短短 14 天内向成熟、功能活跃的软骨细胞分化。然后可以通过多种方法验证分化，包括用爱新蓝或番红染色。

Partial List of Cells Differentiated with hMSC Chondrogenic Differentiation BulletKit^TM Medium*:

Human bone marrow derived stem cells
Human umbilical cord blood derived stem cells
Human umbilical cord matrix derived stem cells
C57BL/6 mice bone marrow derived stem cells
human adipose derived stem cells
Human hamstring tendon derived stem cells

添加 TGF-β3（单独出售）的 hMSC 软骨分化 BulletKitTM 培养基提供了一种分化培养基，可保证诱导骨髓来源的 hMSC 分化为成熟的功能活跃的软骨细胞；每瓶 hMSC 软骨分化 BulletKitTM 培养基都含有足够的分化培养基，可将至少 200 万个骨髓来源的 hMSC [大约 4 个完整的 24 孔板] 完全分化为成熟的功能活跃的软骨细胞。

hMSC 软骨分化培养基以 BulletKitTM 培养基（目录号 PT-3003）的形式提供，其中包括基础培养基和必要的补充剂（添加 TGF-β3 [单独出售，目录号 PT-4124]），用于骨髓来源的 hMSCs 的成软骨分化。基础培养基（Differentiation Basal Medium – Chondrogenic；目录号 PT-3925）和必要的补充剂（HMSC SQ 试剂盒 – 软骨；目录号 PT-4121 和用于 HMSC Chondro diff 的 TGF Beta 3；目录号 PT-4124）也可以单独购买。

只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。

*从客户数据生成的单元格列表。 Lonza 仅常规地将这种培养基与 Lonza 的人骨髓来源的间充质干细胞一起使用，因此只能保证这种培养基与这些细胞一起成功使用。

Content & Storage

Content

1 x hMSC Chondrogenic Basal Medium (PT-3925)

1 x hMSC Chondrogenic SingleQuot Kit (PT-4121)

Related Products

Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124

用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3

Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKit^TM PT-3002

人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002

Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKit^TM PT-3004

人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008

糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5008

DPSC – Human Dental Pulp Stem Cells PT-5025

NHAC-kn – Human Knee Articular Chondrocytes CC-2550

NHAC-kn – 人膝关节软骨细胞 CC-2550

CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216

CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225

CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225

Chondrocyte ReagentPack™ Subculture Reagents CC-3233

软骨细胞 ReagentPack™ 继代培养试剂 CC-3233

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza 00192152,KGM™ Gold 角质形成细胞生长培养基 SingleQuots™ 补充剂和生长因子

Catalog #: 00192152

KGM-GoldTM 角质形成细胞 SingleQuotsTM 试剂盒，人类角质形成细胞培养所需的补充剂和生长因子。

Product Overview

KGM^TM Gold SingleQuot^TM Kit are components sold separately. These are part of the KGM^TM Gold Keratinocyte Growth Medium BulletKit^TM (Catalog Number 192060). The full KGM^TM Gold BulletKit^TM consisting of basal medium and supplements can be purchased together using the catalog number 192060.

KGM^TM Gold BulleKit^TM is a new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures

KGMTM Gold SingleQuotTM 套件是单独出售的组件。这些是 KGMTM Gold 角质形成细胞生长培养基 BulletKitTM（目录号 192060）的一部分。由基础培养基和补充剂组成的完整 KGMTM Gold BulletKitTM 可以使用目录号 192060 一起购买。

KGMTM Gold BulleKitTM 是一种新的稳健且多功能的无血清培养基配方，适用于高密度和低密度 NHEK-正常人表皮角质形成细胞培养

Content & Storage

Content

1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.25 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL

Related Products

KGM™ Gold Keratinocyte Growth Medium SingleQuots™ Supplements and Growth Factors

NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627

NHEK-Ad – 人表皮角质形成细胞，成人，单一供体 00192627

NHEK-Neo – Human Epidermal Keratinocytes – Neonatal, Single Donor 00192907

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled 00192906

NHEK-Neo – 人表皮角质形成细胞，新生儿，汇集 00192906

D-HEK-Ad – Diseased Human Epidermal Keratinocytes, Adult – Diabetes Type II CC-2926

D-HEK-Ad – 患病的人表皮角质形成细胞，成人 – II 型糖尿病 CC-2926

KGM™ Gold Keratinocyte Growth Medium BulletKit™ 00192060

KGM™ 黄金角质形成细胞生长培养基 BulletKit™ 00192060

Data

KGM™ Gold Keratinocyte Growth Medium SingleQuots™ Supplements and Growth Factors

Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.

The growth performance and longevity of Competitor A’s adult keratinocytes grown in Competitor A’s Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.

将竞争对手 A 的成年角质形成细胞在竞争对手 A 的培养基中生长的生长性能和寿命与龙沙在 KGM-Gold™ 生长培养基中生长的成年角质形成细胞进行了比较。尽管竞争对手 A 声称其成年角质形成细胞的总种群倍增 ≥ 25，但实际结果表明，该批次几乎没有获得 19 个 PD。注意竞争对手培养基中第 2 段中存在几个扩大的细胞。在第 4 代（15 PD）结束时，Lonza 的成人角质形成细胞主要由小的增殖细胞组成。

lonza CC-4126,FGM™-2 成纤维细胞生长培养基-2 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4126

FGM^TM-2 Fibroblast SingleQuots^TM Kit

lonza CC-4126,FGM™-2 成纤维细胞生长培养基-2 SingleQuots™ 补充剂和生长因子

Product Overview

FGM^TM -2 SingleQuot^TM Kit are components sold separately and does not include the basal medium. These are part of the FGM^TM -2 Fibroblast Growth Medium BulletKit^TM (Catalog Number CC-3132) which consists of basal medium and supplements packaged together.

FGM^TM-2 Fibroblast Growth Medium-2 BulletKit^TM was developed to support the growth of most primary human fibroblasts including lung and dermal fibroblasts. The kit contains basal medium and supplements packaged together for convenience to culture fibroblasts. FGM^TM-2 Medium contains 2% serum.

Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGM^TM-2 BulletKit^TM Growth Media (catalog no. CC-3132)

FGMTM -2 SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。这些是 FGMTM -2 成纤维细胞生长培养基 BulletKitTM（目录号 CC-3132）的一部分，它由包装在一起的基础培养基和补充剂组成。

FGMTM-2 Fibroblast Growth Medium-2 BulletKitTM 旨在支持大多数原代人类成纤维细胞的生长，包括肺和真皮成纤维细胞。该试剂盒包含包装在一起的基础培养基和补充剂，以方便培养成纤维细胞。 FGMTM-2 培养基含有 2% 血清。

购买我们的肺成纤维细胞培养试剂盒（目录号 CC-2512B）并获得细胞和培养基的折扣价。该试剂盒由冷冻保存的肺成纤维细胞安瓿（货号 CC-2512）和 FGMTM-2 BulletKitTM 生长培养基（货号 CC-3132）组成

Content & Storage

Content

1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 10.00 mL

Related Products

FGM™-2 Fibroblast Growth Medium-2 SingleQuots™ Supplements and Growth Factors

NHDF-Ad – Human Dermal Fibroblasts, Adult CC-2511

NHDF-Neo – Human Dermal Fibroblasts, Neonatal CC-2509

D-HLF-As – Diseased Human Lung Fibroblast Cells – Asthma 00194912

D-HLF-As – 患病的人肺成纤维细胞 – 哮喘 00194912

NHLF – Human Lung Fibroblasts CC-2512

NHLF – 人肺成纤维细胞 CC-2512

D-HLF-COPD – Diseased Human Lung Fibroblast Cell – COPD 00195277

D-HLF-COPD – 患病的人肺成纤维细胞 – COPD 00195277

FGM™-2 Fibroblast Growth Medium-2 BulletKit™ CC-3132

FGM™-2 成纤维细胞生长培养基-2 BulletKit™ CC-3132

lonza2T-110,原代细胞和干细胞,人破骨细胞前体细胞

Catalog #: 2T-110

Osteoclast Precusors, 1 million cells

Product Overview

骨组织的维持和修复主要归因于两种细胞类型：形成新骨组织的成骨细胞和重新吸收骨组织的破骨细胞。骨矿化/骨降解循环中的缺陷会导致许多与骨相关的疾病，例如骨质疏松症。破骨细胞是大的多核细胞，在骨吸收中发挥积极作用。 PoieticsTM 破骨细胞前体细胞系统设计用于高通量应用，以进行骨质疏松症、骨吸收和其他骨相关疾病的研究。来自骨髓或血液的单核细胞充当破骨细胞的前体。传统上，研究人员驱动纯化的单核细胞群成为破骨细胞，然而，在某些情况下，这可能导致低至 10% 的分化效率。相比之下，Lonza 的破骨细胞前体细胞系统在分化时产生高达 50% 的前体向破骨细胞的转化。冷冻保存的 PoieticsTM 人破骨细胞前体细胞 (OCP) 保证每安瓿含有 ≥ 100 万个活细胞，并且在分化后对破骨细胞活性和 TRAP 染色检测呈阳性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。推荐培养基：OCPTMOcteoclast Precursor BulletKitTM 培养基（提供阴性对照/实验培养基，可用作阴性对照[不添加额外的补充剂]或实验培养基[添加研究人员提供的分化补充剂]和阳性对照培养基保证诱导分化为成熟的、功能活跃的破骨细胞；每瓶 OCPTMBulletKitTM 培养基含有足够的分化培养基，可将大约 150 万个破骨细胞前体细胞 [大约 1.5 个完整的 96 孔板] 完全分化成成熟的、功能活跃的破骨细胞）。 OCPTMMedium 以 BulletKitTM 培养基（目录号 PT-8001）的形式提供，其中包括基础培养基和破骨细胞前体的铺板和分化所需的补充剂。基础培养基（OCPTMOsteoclast Precur.Basal Medium；目录号 PT-8201）和必要的补充剂（OCPTMOsteoclast Precur. SingleQuot Kit；目录号 PT-9501）也可以单独购买。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

lonza C2519AS,HUVEC-人脐静脉内皮细胞EGM™-2预先筛选血管生成

Catalog #: C2519AS

Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC) from pooled donors, cultured in EGM^TM-2, pre-screened for Angiogenesis and containing ≥ 500,000 cells

来自汇集供体的人脐静脉内皮细胞 (HUVEC) 的冷冻保存安瓿，在 EGMTM-2 中培养，预先筛选血管生成并含有 ≥ 500,000 个细胞

Product Overview

Lonza 的冷冻保存的 HUVEC 作为冷冻初级产品运输。它们通过 15* 人口倍增得到保证（* HUVEC-XLTM 除外，通过 5 PD 得到保证），表达 CD31/105，von Williebrand 因子 VIII，并且对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

我们的汇集与单一供体选项允许研究人员在他们的实验中研究或排除供体变异性。根据研究，汇总版本通常用于减少供体变异性，而单一供体选项允许研究人员评估特定供体的差异。

HUVEC-XL 大小由 1000 万个细胞/安培组成，通过汇集供体实现。此选项旨在支持需要大量细胞的研究，同时仍为研究人员提供成本效益。

Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?

C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGM^TM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.
C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) – Isolated in EGM^TM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGM^TM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
CC-2935 (single donor) – Isolated in EGM^TM – PlusMedia: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGM^TM Media. No exogenous VEGF.

Benefits

Guaranteed through 15 population doublings
All cells test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis provided for every cell lot

Applications

Would healing
Angiogenesis
Tissue engineering
Inflammation
Oncology
Pharmacology
Vascular modeling
Transfection

Content & Storage

Content

1 x Cryopreserved ampule HUVEC containing ≥ 500,000 cells/vial

Related Products

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis

RAFT™ 24-Well Small Kit, 3D Reagents & 24-Well Plate Absorbers – 12 Reactions 016-1R16

HAEC – Human Aortic Endothelial Cells CC-2535

HCAEC – Human Coronary Artery Endothelial Cells CC-2585

HCAEC – 人冠状动脉内皮细胞 CC-2585

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS

HUVEC – 人脐静脉内皮细胞，单一供体，在 EGM™-2 中，预先筛选血管生成 C2517AS

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517

HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519

HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中

HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935

HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体

lonza00192907,NHEK-Neo-人类表皮角质形成细胞-新生儿单-供体

Catalog #: 00192907

冷冻保存的新生儿正常人表皮角质形成细胞 (NHEK-Neo) 安瓿，单一供体，含有 ≥ 500,000 个细胞

Product Overview

冷冻保存的正常人表皮角质形成细胞新生儿通过 18 个成体细胞的群体倍增得到保证。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

References Citing Lonza’s Keratinocytes:

Protective barrier: Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M, Mlitz V, Gruber F, Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E. Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47.
Skin model: Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207.
Cancer: Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS. EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71.
Human Papillomaviruses (HPV) mediated skin cancer response: An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients. The present study utilized pooled keratinocytes cultured in KGM^{^TM}-Gold Medium from several healthy donors. The data showed that betaPV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165.

Benefits

- Assured for experimental use up to 20 population doublings
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
- Characterized by morphological observation throughout serial passage
- Certificate of Analysis is provided for each cell lot

Applications

Skin modeling
Oncology
Protective barrier
Wound healing
Pigmentation

Content & Storage

Content

1 x Cryopreserved ampule of NHEK-Neo (single donor) containing ≥ 500,000 cells

Related Products

NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504

NHEM-Neo – 人表皮黑色素细胞，新生儿 CC-2504

lonza PT-3238,MSCBM™ 间充质干细胞基础培养基

Catalog #: PT-3238

MSCBMTM 基础培养基，用于培养人骨髓间充质干细胞，440 mL

Product Overview

MSCBM Basal Medium is the basal medium component of the MSCGM human Mesenchymal Stem Cell Growth BulletKit^TM (catalog no. PT-3001), a serum containing medium designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state. The serum used in Lonza’s MSCGM^TMhuman Mesenchymal Stem Cell Growth Medium BulletKit^TM Medium is specifically screened to ensure that the serum neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media).

MSCBM 基础培养基是 MSCGM 人间充质干细胞生长 BulletKitTM（目录号 PT-3001）的基础培养基成分，这是一种含血清的培养基，旨在使人骨髓来源的间充质细胞在未分化状态下增殖。 Lonza 的 MSCGMTMhuman 间充质干细胞生长培养基 BulletKitTM 培养基中使用的血清经过专门筛选，以确保血清既不促进细胞的自发分化，也不抑制细胞向成骨、软骨或脂肪谱系的分化（当用适当的分化培养基适当刺激时）。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Related Products

MSCBM™ Mesenchymal Stem Cell Basal Medium

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001

Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKit^TM PT-3003

人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM PT-3003

Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKit^TM PT-3004

人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004

MSCGM™ SingleQuots™ Supplements and Growth Factors PT-4105

MSCGM™ SingleQuots™ 补充剂和生长因子 PT-4105

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124

用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3

hADSC Human Adipose-Derived Stem Cells PT-5006

hADSC 人体脂肪干细胞 PT-5006

DPSC – Human Dental Pulp Stem Cells PT-5025

DPSC – 人牙髓干细胞 PT-5025

lonza CC-2561,CC-2580,原代细胞和干细胞,SkMC-人体骨骼肌细胞

Catalog #: CC-2561

Cryopreserved ampule of Human Skeletal Muscle Cells (SkMC) containing ≥ 500,000 cells

Product Overview

CloneticsTM 人类骨骼肌细胞 (SkMC) 是从正常供体中分离出来的。 SkMC 是从妊娠组织中分离出来的，通常是从股四头肌或腰大肌组织中分离出来的。

CloneticsTM SkMC 保证通过 15 次群体倍增并且对结蛋白染色呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书 (CoA)。

Applications

Cellular development
Differentiation
Diabetes
Insulin uptake or resistance
Tissue repair
Drug development
Myotube development
Co-culture with other cell types

Content & Storage

Content

1 x Cryopreserved ampule of SkMC containing ≥ 500,000 cells

Related Products

HSMM – Human Skeletal Muscle Myoblasts CC-2580

HSMM – 人体骨骼肌成肌细胞 CC-2580

P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4012

P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4024

P4 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-4024

P4 Primary Cell 4D-Nucleofector™ X Kit S V4XP-4032

P4 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-4032

P4 Primary Cell 96-well Nucleofector™ Kit V4SP-4096

P4 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-4960

P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4002

P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4002

P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4010

P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4010

lonza,CC-2530, CC-2913,HPAEC-人肺动脉内皮细胞

Catalog #: CC-2530

含有 ≥ 500,000 个细胞的正常人肺动脉内皮细胞 (HPAEC) 冻存安瓿

Product Overview

Lonza’s Human Pulmonary Artery Endothelial Cells (HPAEC) and media are convenient and easy to use, allowing the researcher to focus on results. Cryopreserved HPAEC cells are shipped in third passage. Proliferating cells are shipped in fourth passage. Cells isolated from Diabetes Type 1 and 2 are also available.

Clonetics^TM cells, medium and reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.

Lonza 的人肺动脉内皮细胞 (HPAEC) 和培养基使用方便且易于使用，使研究人员能够专注于结果。冷冻保存的 HPAEC 细胞在第三代运输。增殖细胞在第四代运输。也可使用从 1 型和 2 型糖尿病中分离的细胞。

CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。

Benefits

Assured for experimental use up to 15 population doublings
All cells test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Characterized by morphological observation throughout serial passage
Certificate of Analysis provided for every cell lot

Applications

Hypertension
Arteriosclerosis
Inflammation
Oncology
Drug screening

Content & Storage

Content

1 x Cryopreserved ampule of HPAEC containing ≥ 500,000 cells

Related Products

HPAEC – Human Pulmonary Artery Endothelial Cells

D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type II CC-2913

D-PASMC – 患病的人肺动脉平滑肌细胞 – II 型糖尿病 CC-2913

D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type I CC-2915

HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527

SAEC – Human Small Airway Epithelial Cells CC-2547

NHLF – Human Lung Fibroblasts CC-2512

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

BSMC – Human Bronchial Smooth Muscle Cells CC-2576

EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ CC-3162

P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012

P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024

P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032

P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096

P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960

P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002

P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010

Data

HPAEC – Human Pulmonary Artery Endothelial Cells

Human pulmonary artery endothelial cells (CC-2530) were immunohistochemistry labeled with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1088 DyLight 488 from (Vector Laboratories). Staining for CD31/PECAM-1 was conducted using antibody from R&D Systems, BBA7 at 1:100. Secondary antibody used to detect CD31/PECAM-1 was VECTDI2594 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst.

HPAEC – Human Pulmonary Artery Endothelial Cells

用 1:200 使用的血管性血友病因子抗体 (Sigma, F3520) 对人肺动脉内皮细胞 (CC-2530) 进行免疫组织化学标记。使用的二抗是来自 (Vector Laboratories) 的 VECTDI1088 DyLight 488。使用来自 R&D Systems 的抗体 BBA7 以 1:100 进行 CD31/PECAM-1 染色。用于检测 CD31/PECAM-1 的二抗是 VECTDI2594 DyLight 594 (Vector Laboratories)。细胞核用赫斯特染色。

lonza CC-3191,CC-2553,REBM™ 肾上皮细胞生长基础培养基

Catalog #: CC-3191

REBM^TM Renal Epithelial Basal Medium, 500 mL

Product Overview

REBM^TM is a renal epithelial cell growth basal medium sold separately. This can be used with 1 kit of CC-4127 (renal cell growth medium SingleQuots^TM Supplements and Growth Factors). The full REGM^TM BulletKit^TM consisting of basal medium and supplements can be purchased together using the catalog number CC-3190.

REGM^TM Renal Epithelial Cell Growth Medium BulleKit^TM is formulated to maintain and expand most renal epithelial cells. REGM^TM contains 0.5% serum.

REBMTM 是一种肾上皮细胞生长基础培养基，单独出售。这可以与 1 套 CC-4127（肾细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。由基础培养基和补充剂组成的完整 REGMTM BulletKitTM 可以使用目录号 CC-3190 一起购买。

REGMTM 肾上皮细胞生长培养基 BulleKitTM 用于维持和扩增大多数肾上皮细胞。 REGMTM 含有 0.5% 的血清。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products

REBM™ Renal Epithelial Cell Growth Basal Medium

HRCE – Human Renal Cortical Epithelial Cells CC-2554

HRE – Human Renal Epithelial Cells CC-2556

RPTEC – Human Renal Proximal Tubule Epithelial Cells CC-2553

RPTEC – 人肾近端小管上皮细胞 CC-2553

D-RPTEC – Diseased Human Renal Proximal Tubule Epithelial Cells – Diabetes Type II CC-2925

D-RPTEC – 患病的人肾近端小管上皮细胞 – II 型糖尿病 CC-2925

REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190

REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

lonza,lonza CC-4133,EGMTM 内皮细胞生长培养基 SingleQuotsTM 试剂盒

Catalog #: CC-4133

Supplements and growth factors required for culture of Endothelial Cells (basal media sold separately

Product Overview

EGM^TM SingleQuot^TM Kit are components sold separately and does not include the basal medium. These are part of the EGM^TM Endothelial Cell Growth Medium BulletKit^TM (Catalog Number CC-3124) which consists of basal medium and supplements packaged together.

Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow Clonetics^TM Primary Cells.

EGMTM SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。这些是 EGMTM 内皮细胞生长培养基 BulletKitTM（目录号 CC-3124）的一部分，它由包装在一起的基础培养基和补充剂组成。

从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。

The following Endothelial Cell Growth Medium BulletKits^TM are available:

EGM^TM BulletKit^TM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
EGM^TM-Plus BulletKit^TM (CC-5035) – Enhanced version of EGM^TM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGM^TMMedium.
EGM^TM-2 BulletKit^TM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
EGM^TM-2 MV BulletKit^TM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.

Lonza guarantees the performance of Clonetics^TM/Poietics^TM Cells only if appropriate Clonetics^TM/Poietics^TM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

Content & Storage

Content

1 x Green Cap Vial with hEGF 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 Bottle BBE 2.00 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL
1 Bottle FBS 10.00 mL

Related Products

EGMTM Endothelial Cell Growth Medium SingleQuotsTM Kit

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517

HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519

HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中

EGM™ Endothelial Cell Growth Medium BulletKit™ CC-3124

EGM™ 内皮细胞生长培养基 BulletKit™ CC-3124

lonza1M-125,未加工人骨髓25 mL

Catalog #: 1M-125

Fresh Unprocessed Human Bone Marrow, 25 mL

Product Overview

We Find the Donor. You Find the Cure.

At Lonza, we are committed to handling the logistics so you can focus on finding the cure. Providing the research community with fresh unprocessed bone marrow while maintaining the well-being of our donors is at the forefront of Lonza’s approved bone marrow donor program. We have been delivering the fresh unprocessed bone marrow you need for over 20 years so you can focus on the important work behind finding the cure.

在 Lonza，我们致力于处理物流，因此您可以专注于寻找治疗方法。为研究界提供新鲜的未加工骨髓，同时维护我们捐赠者的福祉，这是龙沙批准的骨髓捐赠者计划的最前沿。 20 多年来，我们一直在提供您需要的新鲜未加工骨髓，因此您可以专注于寻找治愈方法背后的重要工作。

More Cells

All research-use, fresh unprocessed bone marrow samples contain greater than 15 million nucleated viable cells per mL, giving you more cells for your money. A total of 100 mL per donor can be ordered in 10 or 25 mL quantities. Up to 100 mL can be ordered from each donor. Please inquire about pricing for large volumes of fresh unprocessed bone marrow.

Fresh Delivery

Unprocessed bone marrow samples are never diluted and are shipped at ambient temperature for next day delivery, so your samples arrive fresh and viable.

Unprocessed bone marrow orders are shipped Monday through Thursday and are typically scheduled 1-2 weeks in advance, depending on donor and volume requirements. International shipments are available, and lead times vary.

Relevant Results

Sample representation is important to ensure you are gathering relevant results for your target population. The Lonza bone marrow donor program has been in operation since 1994. That’s over 20 years of maintaining a large and varied donor pool to enable relevant research results. In addition, we also offer whole blood and bone marrow from the same donor.

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Unprocessed Human Bone Marrow, 25 mL

Related Products

Unprocessed Human Bone Marrow, Fresh, 25 mL

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 100,000 2M-101

人骨髓 CD34+ 祖细胞，冷冻保存，100,000 2M-101

Human Bone Marrow Stromal Cells, 5 Million 2M-302

Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells 2C-101A

人脐带血 CD34+ 祖细胞，冷冻保存，500,000 个细胞 2C-101A

hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

lonza CC-3181,CC-2587,CC-2533,SmBM™ 平滑肌细胞生长基础培养基500 mL

Catalog #: CC-3181

Basal media required for growth of Smooth Muscle Cells, 500 mL (supplements and growth factors sold separately).

Product Overview

SmBM^TM -2 is a Smooth Muscle Cell basal medium sold separately. This can be used with 1 kit of CC-4149 (smooth muscle cell growth medium SingleQuots^TM Supplements and Growth Factors). The full SmGM^TM -2 BulletKit^TM consisting of basal medium and supplements can be purchased together using the catalog number CC-3182.

SmGM^TM-2 Smooth Muscle Growth Medium-2 is a robust medium that was developed for the maintenance and expansion of human smooth muscle cells from various tissue origins including cardiac, airway, prostate and more. This kit is also utilized to support the maintenance of intestinal cells. SmGM^TM-2 medium contains 5% FBS.

Clonetic^TM Media Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems.

SmBMTM -2 是一种平滑肌细胞基础培养基，单独出售。这可以与 1 套 CC-4149（平滑肌细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。由基础培养基和补充剂组成的完整 SmGMTM -2 BulletKitTM 可以使用目录号 CC-3182 一起购买。

SmGMTM-2 Smooth Muscle Growth Medium-2 是一种强大的培养基，用于维持和扩增来自各种组织来源的人类平滑肌细胞，包括心脏、气道、前列腺等。该试剂盒还用于支持肠道细胞的维持。 SmGMTM-2 培养基含有 5% FBS。

CloneticTM 培养基产品专为支持人类和动物原代细胞的生长而设计。媒体系统已针对各种细胞系统专门开发和优化。

Content & Storage

Content

1 x Bottle SmBM Basal Medium, 500 mL

Related Products

SmBM™ Smooth Muscle Cell Growth Basal Medium, 500 mL

BdSMC – Human Bladder Smooth Muscle Cells CC-2533

D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma 00194850

D-BSMC-COPD – Diseased Human Bronchial Smooth Muscle Cells – COPD 00195274

UASMC – Human Umbilical Artery Smooth Muscle Cells CC-2579

AoSMC – Human Aortic Smooth Muscle Cells CC-2571

BSMC – Human Bronchial Smooth Muscle Cells CC-2576

UtSMC – Human Uterine Smooth Muscle Cells CC-2562

CASMC – Human Coronary Artery Smooth Muscle Cells CC-2583

PrSMC – Human Prostate Smooth Muscle Cells CC-2587

D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type II CC-2913

InMyoFib – Human Intestinal Myofibroblasts CC-2902

D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type I CC-2914

D-AoSMC – 患病的人主动脉平滑肌细胞 – I 型糖尿病 CC-2914

D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type I CC-2915

D-PASMC – 患病的人肺动脉平滑肌细胞 – I 型糖尿病 CC-2915

D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type II CC-2916

D-AoSMC – 患病的人主动脉平滑肌细胞 – II 型糖尿病 CC-2916

D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type I CC-2917

D-CASMC – 患病的人冠状动脉平滑肌细胞 – I 型糖尿病 CC-2917

D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type II CC-2918

D-CASMC – 患病的人冠状动脉平滑肌细胞 – II 型糖尿病 CC-2918

lonza,HUCLS,原代细胞和干细胞,星状细胞人类冷冻保存第 1 代

Catalog #: HUCLS

Human Cryopreserved Stellate cells derived from liver

Product Overview

Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.

Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state.

Human Cryopreserved Stellate Cells, 100K cells/vial, are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.

Human Stellate Growth Medium (Catalog MCST250) is available to support optimal culture health and growth characteristics of stellate cells.

星状细胞是肝脏的一种常驻细胞类型，主要功能是储存类维生素A。作为对肝损伤的反应，星状细胞迅速失去储存的类维生素A并分化成增殖的成纤维细胞样细胞，开始沉积胶原蛋白基质。这种活动会导致肝脏中胶原蛋白的堆积，最终导致肝硬化。

星状细胞可以从肝组织中分离、富集并放入细胞培养物中。当放入培养物中时，星状细胞开始立即分化成活化状态。

人冷冻保存的星状细胞，100K 细胞/瓶，是从人肝分离物中富集的星状细胞，并生长到 >80% 的汇合度，然后冷冻保存。

人星状生长培养基（目录 MCST250）可用于支持星状细胞的最佳培养健康和生长特性。

Characterization performed:

Post-thaw plating to observe characteristic stellate morphology.
Flow cytometry using antibodies for Vinculin, Vimentin, and α-SMA

Benefits

Low Passage
High activation potential

Applications

ADMETox
Liver Disease
NASH

Content & Storage

Content

Cryo Vial

100,000 cells/ vial

Stellate cells, human cryopreserved, passage 1

lonza CC-5024,CC-5034,原代细胞和干细胞,HEPES 缓冲盐溶液500 mL

Catalog #: CC-5024

HEPES Buffered Saline Solution for use in cell culture, 500 mL

Product Overview

HEPES 缓冲盐水溶液 (BSS) 用于中和生长培养基中的复杂蛋白质，这些蛋白质可能在胰蛋白酶消化过程中使胰蛋白酶失活。

填充体积为 100 mL 的 HEPES BSS 是 ReagentPackTM Subculture 试剂盒（目录号 CC-5024）的一个组件。 100 mL 容量也单独出售（目录号 CC-5022）。

Content & Storage

Content

1 x Bottle HEPES Buffered Solution, 500 mL

Related Products

ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

HEPES Buffered Saline Solution – 100 ml CC-5022

HEPES 缓冲盐溶液 – 100 ml CC-5022

lonza R-CM-561, CC-4516,大鼠新生儿心肌细胞

Catalog #: R-CM-561

Cryopreserved ampule of Rat Neonatal Cardiomyocytes containing ≥4 million cells

Product Overview

新生儿心室大鼠心肌细胞 (P1-3) 是通过标准化方法制备的高质量原代心肌细胞，解冻后即可立即培养。

一瓶心肌细胞含有 ≥ 400 万个活细胞，纯度≥ 85%。这将使用推荐的铺板密度和培养基接种到 24 孔板的 10 个或更多孔和 96 孔板的 26 个或更多孔中。解冻和培养后，您将获得高活力，具有出色的形态和连接性，并且细胞将在培养 24 小时时显示跳动。

每批这些细胞的功能性合胞体形成检测呈阳性，肌动蛋白染色呈阳性。细胞的支原体和细菌检测也呈阴性。原代心肌细胞需要合适的底物才能粘附和存活。优选的底物是硝酸纤维素。

Recommended medium: RCGM^TM BulletKit^TM (CC-4515)*

*RCGMTM 大鼠心肌细胞生长培养基专为培养中的大鼠新生心肌细胞的生长和存活而配制。龙沙为客户提供经过充分验证和优化的心肌细胞培养基和生长因子，以补充我们的 CloneticsTM 大鼠心肌细胞 R-CM-561，作为研究心脏生物学/毒理学的完整系统。

Benefits

Ready-To-Use
No tissue dissection or animal handling required
Increased data reproducibility
Characteristic morphology and staining tests confirm tissue origin and cell type
Negative for mycoplasma, bacteria, yeast and fungi
Certificate of Analysis (CoA) is available for every batch

Applications

Cardiovascular diseases
Hypertension
Inflammation
Cell contraction
Cardiac cell physiology
Ion channel flux
Cardiac lipid metabolism and lipotoxicity
Cardiac ischemia and injury
Cardiac apoptosis
Cardiac cytotoxicity
Tissue engineering

Content & Storage

Content

≥ 4 million cryopreserved cells per ampule

Related Products

RCGM™ Rat Cardiomyocyte Growth Medium SingleQuots™ Supplements and Growth Factors CC-4516

RCGM™ 大鼠心肌细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4516

Rat Cardiomyocyte Growth Medium (rCGM) BulletKit^TM CC-4515

大鼠心肌细胞生长培养基 (rCGM) BulletKitTM CC-4515

rCBM™Rat Cardiomyocyte Basal Medium CC-3275

rCBM™大鼠心肌细胞基础培养基 CC-3275

lonza,CC-2904,原代细胞和干细胞,NHCF-V-人心室心脏成纤维细胞

Catalog #: CC-2904

Cryopreserved ampule of Normal Human Ventricular Cardiac Fibroblasts (NHCF-V) containing ≥ 500,000 cells

Product Overview

Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle. Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2.

Clonetics^TM NHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. Clonetics^TM AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.

人类心脏相关成纤维细胞包括从升主动脉或降主动脉外部的被膜分离的主动脉外膜成纤维细胞 (AoAF) 和从心房或心室分离的心脏成纤维细胞 (NHCF-A 和 -V)。细胞是从正常的成人心脏组织中分离出来的。冷冻保存的细胞在第 2 代运送。

CloneticsTM NHCF-V 和 -A 通过 5 次群体倍增得到保证，解冻后表达 ≥90% 的胶原蛋白 I 和 ≤10% 的 Von Willibrand 因子 VIII。 CloneticsTM AoAF 通过 10 次群体倍增得到保证，并且对 α 平滑肌肌动蛋白呈阴性。所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书 (CoA)。

Benefits

Assured for experimental use up to 15 population doublings
Express ≥90% collagen I and ≤10% Von Willibrand factor VIII
All cells test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis provided for every cell lot

Applications

Vascular diseases
Neointimal formation
Vascular remodeling
Atherosclerosis
Hypertension
Myocardial repair
Extracellular maintenance during cardiac cell / tissue growth
Cardiac Fibrosis Paracrine signalling
Toxicology
Gene expression

Content & Storage

Content

1 x Cryopreserved ampule NHCF-V containing ≥ 500,000 cells/vial

Related Products

NHCF-V – Human Ventricular Cardiac Fibroblasts

AoAF – Human Aortic Adventitial Fibroblasts CC-7014

AoAF – 人主动脉外膜成纤维细胞 CC-7014

NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903

HAEC – Human Aortic Endothelial Cells CC-2535

HAEC – 人主动脉内皮细胞 CC-2535

AoSMC – Human Aortic Smooth Muscle Cells CC-2571

AoSMC – 人主动脉平滑肌细胞 CC-2571

FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™ CC-4526

FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™ CC-4526

lonza,lonza CC-5022,原代细胞和干细胞,HEPES 缓冲盐溶液

Catalog #: CC-5022

HEPES Buffered Saline Solution, 100 mL

Overview

Product Overview

HEPES-Buffered Saline Solution (BSS), is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization.

HEPES-BSS is a component of the ReagentPack^TM Subculture kit (catalog number CC-5034). It is also available in a 500 mL size (catalog number CC-5024)

HEPES 缓冲盐溶液 (BSS) 用于中和生长培养基中的复杂蛋白质，这些蛋白质可能会在胰蛋白酶消化过程中使胰蛋白酶失活。

HEPES-BSS 是 ReagentPackTM Subculture 试剂盒（目录号 CC-5034）的一个组件。它也有 500 mL 规格（目录号 CC-5024）

Content & Storage

Content

1 x Bottle HEPES Buffered Solution 100 mL

lonza,lonza CC-3166,CC-2555,CC-3165,PrEGM™ 前列腺上皮细胞生长培养基 BulletKit™

Catalog #: CC-3166

Culture system containing PrEBM^TM Basal Medium (CC-3165) and PrEGM^TM SingleQuots^TM Supplements (CC-4177) required for growth of Prostate Epithelial Cells

含有前列腺上皮细胞生长所需的 PrEBMTM 基础培养基 (CC-3165) 和 PrEGMTM SingleQuotsTM 补充剂 (CC-4177) 的培养系统

Product Overview

Prostate Epithelial Cell Growth Medium has been specially formulated for the maintenance and expansion of human prostate epithelial cells. The BulletKit^TM consists of basal medium and supplements packaged together for convenience. PrEGM^TM Medium contains no serum.

前列腺上皮细胞生长培养基专为人前列腺上皮细胞的维持和扩增而配制。 BulletKitTM 由基础培养基和补充剂组成，方便起见。 PrEGMTM 培养基不含血清。

Content & Storage

Content

1 x PrEBM^TM Basal Medium (CC-3165), 500 mL

1 x PrEGM^TM SingleQuots^TM Supplement Pack (CC-4177) containing:

1 x Orange Cap Vial with BPE, 2.00 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL

Related Products

PrEGM™ Prostate Epithelial Cell Growth Medium BulletKit™

PrEC – Human Prostate Epithelial Cells CC-2555

PrEC – 人前列腺上皮细胞 CC-2555

PrEBM™ Prostate Epithelial Cell Growth Basal Medium CC-3165

PrEBM™ 前列腺上皮细胞生长基础培养基 CC-3165

PrEGM™ Prostate Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors CC-4177

PrEGM™ 前列腺上皮细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4177

lonza,CC-3111,原代细胞和干细胞,KGM™ 角质形成细胞生长培养基 BulletKit™

Catalog #: CC-3111

KGM^TM Keratinocyte Growth Medium BulletKit^TM culture system contains KBM^TM Basal Medium (CC-3101) and KGM^TM SingleQuot supplements (CC-4131) required for growth of human keratinocytes.

KGMTM 角质形成细胞生长培养基 BulletKitTM 培养系统包含人类角质形成细胞生长所需的 KBMTM 基础培养基 (CC-3101) 和 KGMTM SingleQuot 补充剂 (CC-4131)。

Product Overview

KGM^TM Keratinocyte Growth Medium has been optimized for the proliferation of keratinocytes in a serum-free environment.

Did you know our customers have used KGM^{^TM} Media also to culture immortalized corneal epithelial cells? Check out Jolly, et. al. publication that provides some guidelines to do this.

KGMTM 角质形成细胞生长培养基已针对角质形成细胞在无血清环境中的增殖进行了优化。

您知道我们的客户也使用 KGMTM 培养基培养永生化角膜上皮细胞吗？看看 Jolly 等。人。提供了一些指导方针的出版物。

Content & Storage

Content

1 x KBM^TM(CC-3101) Basal Medium, 500 mL

1 x KGM^TM (CC-4131) Singlequot Supplement Pack containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL

lonza,lonza PT-5025,原代细胞和干细胞,DPSC-人类牙髓干细胞

Catalog #: PT-5025

Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells

Product Overview

PoieticsTM 人类牙髓干细胞 (DPSC) 是从捐赠者的“智齿”拔牙过程中收集的成年第三磨牙中分离出来的。这些细胞表现出与骨髓来源的间充质干细胞非常相似的表型和功能特征。据报道，人类牙髓干细胞 (DPSC) 可分化出许多不同的谱系，包括软骨形成、成骨、脂肪形成和神经细胞。

人牙髓干细胞 (DPSC) 已在初级传代时冷冻保存，并且 DPSC 牙髓干细胞 BulletKitTM 培养基已针对细胞维持和扩增进行了优化。

冷冻保存的 PoieticsTM 牙髓干细胞 (DPSC) 保证通过 10 次群体倍增，表达 CD105、CD166、CD29、CD90 和 CD73，并且不表达 CD34、CD45 和 CD133。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Recommended Growth Media and Reagents

DPSC Dental Pulp Stem Cell BulletKit^TM Medium, PT-3005

The DPSC Dental Pulp Stem Cell BulletKit^TM Medium is offered as a BulletKit^TM Medium (catalog no. PT-3005) which includes both the basal media and the necessary supplements for proliferation of human dental pulp derived mesenchymal stem cells. The basal medium (DPSC Basal Medium; catalog no. PT-3927) and the necessary supplements (DPSC SingleQuot Kit; catalog no. PT-4516) can also be purchased individually.

DPSC 牙髓干细胞 BulletKitTM 培养基以 BulletKitTM 培养基（目录号 PT-3005）的形式提供，其中包括基础培养基和人牙髓衍生间充质干细胞增殖所需的补充剂。基础培养基（DPSC 基础培养基；目录号 PT-3927）和必要的补充剂（DPSC SingleQuot 试剂盒；目录号 PT-4516）也可以单独购买。

Applications

Toxicology/drug screening
Regenerative medicine/dentistry/cell therapy
Muscular Dystrophy
Corneal replacement therapy
Cardiovascular disease
Comparative stem cell research

Content & Storage

Content

1 x Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells

Related Products

Human Dental Pulp Stem Cell Basal Medium PT-3927

Human Dental Pulp Stem Cell SingleQuots™ Supplements and Growth Factors PT-4516

人牙髓干细胞 SingleQuots™ 补充剂和生长因子 PT-4516

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001

MSCGM™ 间充质干细胞生长培养基 BulletKit™ PT-3001

Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKit^TM PT-3002

人间充质干细胞 (hMSC) 成骨分化培养基 BulletKitTM PT-3002

Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKit^TM PT-3003

人间充质干细胞 (hMSC) 软骨分化培养基 BulletKitTM PT-3003

Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKit^TM PT-3004

人间充质干细胞 (hMSC) 脂肪分化培养基 BulletKitTM PT-3004

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124

用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3

hADSC Human Adipose-Derived Stem Cells PT-5006

hADSC 人体脂肪干细胞 PT-5006

lonza CC-7030,CC-2535,CC-2585,HMVEC-C-人心脏微血管内皮细胞

Catalog #: CC-7030

Cryopreserved ampule of Human Cardiac Microvascular Endothelial Cells (HMVEC-C) containing ≥ 500,000 cells

含有 ≥ 500,000 个细胞的人心脏微血管内皮细胞 (HMVEC-C) 冻存安瓿

Product Overview

Human Cardiac Microvascular Endothelial Cells (HMVEC-C) are isolated from normal and diabetic donors. Additional donor characteristics including hypertension, or smoking history may be available by contacting our Scientific Support Team.

Clonetics^TM Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system. Cryopreserved Human Cardiac Microvascular Endothelial Cells are shipped in third passage.

Cryopreserved Human Cardiac Microvascular Endothelial Cells (CC-7030) are guaranteed through 10 population doublings, express CD31/105, von Williebrand Factor VIII, and are positive for acetyated low density lipoprotein uptake. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

人心脏微血管内皮细胞 (HMVEC-C) 是从正常和糖尿病供体中分离出来的。联系我们的科学支持团队，可以获得其他捐赠者特征，包括高血压或吸烟史。

CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。冷冻保存的人心脏微血管内皮细胞在第三代运输。

冷冻保存的人类心脏微血管内皮细胞 (CC-7030) 保证通过 10 次群体倍增，表达 CD31/105、von Williebrand 因子 VIII，并且对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Benefits

Guaranteed through 15 population doublings
Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Related Products

HMVEC-C – Human Cardiac Microvascular Endothelial Cells

HAEC – Human Aortic Endothelial Cells CC-2535

HAEC – 人主动脉内皮细胞 CC-2535

HCAEC – Human Coronary Artery Endothelial Cells CC-2585

HCAEC – 人冠状动脉内皮细胞 CC-2585

EGM^TM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit^TM CC-3202

EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM CC-3202

P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012

P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024

P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032

P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032

P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096

P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960

P5 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-5960

P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002

P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5002

P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010

P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5010

lonza 00192906,CC-2504,NHEK-Neo-人表皮角质形成细胞，新生儿，混合

Catalog #: 00192906

Cryopreserved Human Neonatal Epidermal Keratinocytes, Pooled, ≥ 500,000 cells

Product Overview

Cryopreserved Normal Human Epidermal Keratinocytes from pooled donros are guaranteed through 18 population doublings for adult and neonatal cells.

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

References with Lonza’s Keratinocytes:

Protective barrier: Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M, Mlitz V, Gruber F, Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E. Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47.

Skin model: Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207.

Cancer: Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS. EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71.

Human Papillomaviruses (HPV) mediated skin cancer response: An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients. The present study utilized pooled keratinocytes cultured in KGM^{^TM}-Gold Medium from several healthy donors. The data showed that beta PV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165.

Content & Storage

Content

1 x Cryopreserved ampule of Human Neonatal Epidermal Keratinocytes containing ≥ 500,000 cells

Related Products

NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled

NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504

NHEM-Neo – 人表皮黑色素细胞，新生儿 CC-2504

P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 Primary Cell 4D-Nucleofector^® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

Data

Growth Curve of NHEK Culture

The growth curve of the Neonatal Human Epidermal Keratinocyte culture displayed in the image shows the expected doubling time of 1 population doubling per 24 hours in the log phase of within the first 2 days of culture, but significantly slows down after 2.5 days and comes finally to a growth stop.

图中显示的新生儿人表皮角质形成细胞培养物的生长曲线显示，在培养的前 2 天的对数阶段，每 24 小时 1 个种群的预期倍增时间，但在 2.5 天后显着减慢，最终达到增长停止。

lonza,CC-4149,SmGM™-2 平滑肌细胞生长培养基-2 SingleQuots™ 补充剂和生长因子

Catalog #: CC-4149

SmGM^TM-2 Smooth Muscle SingleQuots^TM Kit, Supplements and growth factors required for culture of Human Smooth Muscle Cells (basal media sold separately).

Product Overview

SmGMTM -2 SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。这些是 SmGMTM -2 平滑肌细胞生长培养基 BulletKitTM（目录号 CC-3182）的一部分。由基础培养基和补充剂组成的完整 SmGMTM -2 BulletKitTM 可以使用目录号 CC-3182 一起购买。
SmGMTM-2 Smooth Muscle Growth Medium-2 是一种强大的培养基，用于维持和扩增来自各种组织来源的人类平滑肌细胞，包括心脏、气道、前列腺等。该试剂盒还用于支持肠道细胞的维持。 SmGMTM-2 培养基含有 5% FBS。
CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。媒体系统已针对各种细胞系统专门开发和优化。

Content & Storage

Content

1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 1.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 25.00 mL
1 x Green Cap Vial with hEGF 0.50 mL

Related Products

SmGM™- 2 Smooth Muscle Cell Growth Medium-2 SingleQuots™ Supplements and Growth Factors

D-BSMC-As – Diseased Human Bronchial Smooth Muscle Cells – Asthma 00194850

D-BSMC-COPD – Diseased Human Bronchial Smooth Muscle Cells – COPD 00195274

BdSMC – Human Bladder Smooth Muscle Cells CC-2533

AoSMC – Human Aortic Smooth Muscle Cells CC-2571

UtSMC – Human Uterine Smooth Muscle Cells CC-2562

BSMC – Human Bronchial Smooth Muscle Cells CC-2576

UASMC – Human Umbilical Artery Smooth Muscle Cells CC-2579

PrSMC – Human Prostate Smooth Muscle Cells CC-2587

CASMC – Human Coronary Artery Smooth Muscle Cells CC-2583

InMyoFib – Human Intestinal Myofibroblasts CC-2902

D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type II CC-2913

D-PASMC – 患病的人肺动脉平滑肌细胞 – II 型糖尿病 CC-2913

D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type I CC-2914

D-AoSMC – 患病的人主动脉平滑肌细胞 – I 型糖尿病 CC-2914

D-PASMC – Diseased Human Pulmonary Artery Smooth Muscle Cells – Diabetes Type I CC-2915

D-AoSMC – Diseased Human Aortic Smooth Muscle Cells – Diabetes Type II CC-2916

D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type I CC-2917

D-CASMC – 患病的人冠状动脉平滑肌细胞 – I 型糖尿病 CC-2917

D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type II CC-2918

D-CASMC – 患病的人冠状动脉平滑肌细胞 – II 型糖尿病 CC-2918

lonza,lonza CC-2581,CC-2512,CC-2527,PASMC-人肺动脉平滑肌细胞

Catalog #: CC-2581

Cryopreserved ampule of Pulmonary Artery Smooth Muscle Cells (PASMC) containing ≥ 500,000 cells

含有 ≥ 500,000 个细胞的肺动脉平滑肌细胞 (PASMC) 冻存安瓿

Product Overview

Human Pulmonary Artery Smooth Muscle Cells (PASMC) are shipped in 3rd passage.

Human Pulmonary Artery Smooth Muscle Cells are guaranteed for 15 population doublings and to stain positive for alpha smooth muscle actin and negative for von Willebrand Factor VIII after differentiation.

人肺动脉平滑肌细胞 (PASMC) 在第 3 代运输。

人肺动脉平滑肌细胞保证 15 次群体倍增，分化后 α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 染色呈阴性。

所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书 (CoA)。

Applications

Stroke
Diabetes
Vascular research
Artherosclerosis
Hypertension

Content & Storage

Content

1 x Cryopreserved ampule PASMC containing ≥500,000 cells/vial

Related Products

PASMC - Human Pulmonary Artery Smooth Muscle Cells

HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527

SAEC – Human Small Airway Epithelial Cells CC-2547

NHLF – Human Lung Fibroblasts CC-2512

NHLF – 人肺成纤维细胞 CC-2512

BSMC – Human Bronchial Smooth Muscle Cells CC-2576

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1024

P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1002

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010

PASMC - Human Pulmonary Artery Smooth Muscle Cells

PASMC – Human Pulmonary Artery Smooth Muscle Cells

Human pulmonary artery smooth muscle cells(CC-2581) were stained with 1:1500 dilution of Anti Alpha-Smooth Muscle Actin dilution of (CBL171, Sigma). Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at a 1:200 dilution. Nuclei were stained with Hoechst . In multi-well culture, PAMCSs appeared typically epitheloid (polygonal) forming monolayers of very low-level alpha-actin expressing cells. Rarely, a few intensely alpha-actin positive cells (arrows) were evident. These displayed distinct fusiform-elongated morphology.

PASMC – Human Pulmonary Artery Smooth Muscle Cells

Cells are isolated from human pulmonary artery and expanded prior to freezing (CC-2581). When plated & expanded, the cells express little to no alpha-actin unless first stimulated to differentiate.Cells were stained with Anti Alpha-Smooth Muscle Actin Chemicon (Millipore) CBL171 at 1:1500 dilution. Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at 1:200 dilution. Nuclei were stained with Hoechst.

从人肺动脉中分离细胞并在冷冻前扩增 (CC-2581)。当铺板和扩增时，除非首先刺激分化，否则细胞几乎不表达 α-肌动蛋白。细胞用 1:1500 稀释度的抗 α-平滑肌肌动蛋白化学 (Millipore) CBL171 染色。使用 1:200 稀释度的第二驴多克隆抗体 Alexa Fluor Do 抗 Mo 594 Invitrogen (Thermo Fisher) A21203 进行免疫荧光分析。细胞核用赫斯特染色。

lonza,CC-4461,R-CB-503,CC-3256,PNGM™ 原代神经元生长培养基 BulletKit™

Catalog #: CC-4461

含有 PNBMTM 基础培养基 (CC-3256) 和 PNGMTM SinglequotsTM 生长补充剂 (CC-4462) 的培养系统，是啮齿动物原代神经元生长所需的

Product Overview

Clonetics^TM PNGM^TM Primary Neuron Growth Medium Kit (PNGM^TM) was specifically developed for culturing embryonic primary rat and mouse neurons. The media kit is recommended for use with all of our primary rat and mouse neurons.

CloneticsTM PNGMTM 原代神经元生长培养基试剂盒 (PNGMTM) 专为培养胚胎原代大鼠和小鼠神经元而开发。建议将媒体套件与我们所有的主要大鼠和小鼠神经元一起使用。

Benefits

Serum-free medium
Allows for long-term maintenance and growth of neuronal cells
Promotes development of high quality neuronal networks without the need of an astrocyte feeder layer

Content & Storage

Content

1 x PNBM^TM Basal Medium (CC-3256), 200 mL

1 x PNGM^TM Singlequots^TM Growth Supplement Pack (CC-4462)

Related Products

PNGM™ Primary Neuron Growth Medium BulletKit™

PNBM^TM Primary Neuron Basal Medium, 200 mL CC-3256

PNGM™ Primary Neuron Growth Medium SingleQuots™ Supplements and Growth Factors CC-4462

Mouse C57 Brain Striatum Neurons M-CP-302

Mouse CD1 Brain Striatum Neurons M-CP-402

Mouse C57 Brain Cortex Neurons M-CX-300

Mouse CD1 Brain Cortex Neurons M-CX-400

Mouse Brain CD1 Hippocampus Neurons M-HI-401

Rat Cerebellar Granular Neurons R-CB-503

大鼠小脑颗粒神经元 R-CB-503

Rat Brain Striatum Neurons R-CP-502

Rat Brain Cortex Neurons R-CX-500

Rat Neonatal Dorsal Root Ganglion (DRG) Neurons R-DRG-505

大鼠新生儿背根神经节 (DRG) 神经元 R-DRG-505

Rat Brain Hippocampus Neurons R-HI-501

大鼠脑海马神经元 R-HI-501

Rat Brain Hypothalamus Neurons R-HTH-507

大鼠脑下丘脑神经元 R-HTH-507

PNGM™ Primary Neuron Growth Medium BulletKit™

ViaLightPlus Assay in PNGM™ Media of Rat Cortical Neurons

Comparison of survival of Clonetics™ Primary Rat Cortical Neurons cultured in PNGM™ Medium vs. Company A’s medium. Cryopreserved cells were thawed and plated in laminin-coated, 96 well plates at ~1000 cells per square mm (40% of the recommended seeding density) using either PNGM™ Medium or Company A’s medium. Cells were cultured without feeding for six days, at which time cell viability was assessed using the ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay Kit. Results represent the average of 8 experiments.

在 PNGM™ 培养基与 A 公司培养基中培养的 Clonetics™ 原代大鼠皮质神经元的存活率比较。使用 PNGM™ 培养基或 A 公司的培养基，将冷冻保存的细胞解冻并接种在层粘连蛋白涂层的 96 孔板中，每平方毫米约 1000 个细胞（推荐接种密度的 40%）。细胞在不喂食的情况下培养六天，此时使用 ViaLight™ Plus 细胞增殖和细胞毒性生物测定试剂盒评估细胞活力。结果代表 8 次实验的平均值。

lonza PT-4505,原代细胞和干细胞,ADSC 生长培养基 BulletKit™

Catalog #: PT-4505

含有脂肪干细胞生长所需的 ADSC 基础培养基 (PT-3273) 和 ADSC-GM SinglequotsTM Supplement Kit (PT-4503) 的培养系统

Product Overview

干细胞具有作为未分化细胞类型增殖的独特能力，然后在适当刺激时分化成多个细胞谱系。 Lonza 的 ADSC 人脂肪来源的干细胞生长 BulletKitTM 培养基是一种含有血清的培养基，旨在增殖处于未分化状态的人脂肪来源的间充质干细胞（正常和患病）。

Lonza 的 ADSC 人脂肪衍生干细胞生长 BulletKitTM 培养基中使用的血清经过专门筛选，以确保血清既不促进细胞自发分化，也不抑制细胞分化为成骨、软骨或脂肪谱系（当用适当的分化培养基适当刺激时） .

ADSC 人脂肪源性干细胞生长 BulletKitTM 培养基以 BulletKitTM 培养基（目录号 PT-4505）的形式提供，其中包括基础培养基和人脂肪源性间充质干细胞增殖所需的补充剂。基础培养基（ADSC 基础培养基；目录号 PT-3273）和必要的补充剂（ADSC-GM SQ 试剂盒；目录号 PT-4503）也可以单独购买。

Content & Storage

Content

1 x ADSC Basal Medium (catalog no. PT-3273)
1 x ADSC SingleQuots^TM Growth Supplement Kit (catalog no. PT-4503)

Related Products

hADSC Human Adipose-Derived Stem Cells PT-5006

ADSC – Apidose-Derived Stem Cells Growth Medium SingleQuots™ Supplements and Growth Factors PT-4503

ADSC Basal Medium PT-3273

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008

hMSC – Human Mesenchymal Stem Cells PT-2501

MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001

Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKit^TM PT-3002

Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKit^TM PT-3003

Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKit^TM PT-3004

PGM-2^TM Preadipocyte Growth Medium-2 BulletKit^TM PT-8002

PGM-2TM 前脂肪细胞生长培养基-2 BulletKitTM PT-8002

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit PT-4124

用于 hMSC 软骨分化试剂盒 PT-4124 的 TGF Beta 3

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza,R-DRG-505,CC-4461,R-EDRG-515,大鼠新生儿背根神经节 (DRG) 神经元

Catalog #: R-DRG-505

Cryopreserved ampule of Rat Neonatal DRG Neurons containing ≥ 200,000 cells

Product Overview

即用型 CloneticsTM 大鼠背根神经节神经元是通过标准化方法制备的高质量感觉神经元的悬浮液，可立即进行培养。

每瓶背根神经节细胞在 0.25 ml 悬浮液中含有大约 200,000 个细胞。电镀后最初几天会发生细胞死亡，并观察到碎片。这很正常。培养约 4 天后，细胞将形成神经突网络，到第 7 天，碎片将最少。

在没有有丝分裂抑制剂的情况下，神经元倾向于聚集（神经节化）并从底物上脱离。神经节是形成神经中枢的一组神经细胞，尤其是位于大脑或脊髓外的神经细胞。

背根神经节，也称为脊神经节，是每个脊神经节段神经后根的神经节，包含单极初级感觉神经元的细胞体。背根神经节细胞是假单极细胞。假单极细胞有 2 个轴突，而不是一个轴突和树突。一个轴突向脊髓中央延伸；另一个轴突向皮肤或肌肉延伸。

推荐介质：PNGMTM BulletKitTM(CC-4461)

Benefits

Ready-To-Use
No tissue dissection or animal handling required
Increased data reproducibility
Characteristic morphology and staining tests confirm tissue origin and cell type
Negative for mycoplasma, bacteria, yeast and fungi
Certificate of Analysis (CoA) is available for every batch

Applications

Neurogenesis
Neurotransmitter Function
Gene Expression
Signaling Pathways
Electrophysiology
Neurotoxicity
Drug/Compound Screening
Advanced Cell Culture Models

Content & Storage

Content

≥200,000 cryopreserved cells per ampule

Related Products

Rat Neonatal Dorsal Root Ganglion (DRG) Neurons

PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons R-EDRG-515

大鼠胚胎背根神经节 (eDRG) 神经元 R-EDRG-515

P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012

P3 Primary Cell 4D-Nucleofector^® X Kit L V4XP-3024

P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-2583,lonza CC-2585,CC-2917,CASMC-人冠状动脉平滑肌细胞

Catalog #: CC-2583

CASMC, Coronary Artery Smooth Muscle Cells

Product Overview

人血管平滑肌细胞来自多种组织类型，包括主动脉 (AoSMC)、冠状动脉 (CASMC)、肺动脉 (PASMC) 和脐动脉 (UASMC)。冷冻保存的细胞在第 3 代运输。

CloneticsTM 人主动脉平滑肌细胞保证 15 次群体倍增，分化后 α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 染色呈阴性。所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书 (CoA)。

Applications

Stroke
Diabetes
Vascular research
Artherosclerosis
Hypertension

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products

CASMC – Human Coronary Artery Smooth Muscle Cells

HCAEC – Human Coronary Artery Endothelial Cells CC-2585

HCAEC – 人冠状动脉内皮细胞 CC-2585

D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type II CC-2922

D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type I CC-2917

D-CASMC – 患病的人冠状动脉平滑肌细胞 – I 型糖尿病 CC-2917

D-CASMC – Diseased Human Coronary Artery Smooth Muscle Cells – Diabetes Type II CC-2918

D-HCAEC – Diseased Human Coronary Artery Endothelial Cells – Diabetes Type I CC-2921

D-HCAEC – 患病的人冠状动脉内皮细胞 – I 型糖尿病 CC-2921

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1024

P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1002

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

lonza,lonza PT-8002,PT-5022,PGM-2TM 前脂肪细胞生长培养基-2 BulletKitTM

Catalog #: PT-8002

Culture system containing PGM^TM-2 Basal Medium (PT-8202) and PGM^TM-2 SingleQuots^TM supplements (PT-9502) required for growth of Preadipocytes

包含前脂肪细胞生长所需的 PGMTM-2 基础培养基 (PT-8202) 和 PGMTM-2 SingleQuotsTM 补充剂 (PT-9502) 的培养系统

Product Overview

PGMTM -2 Predipocyte Growth Medium-2 BulletKitTM 提供增殖培养基和分化培养基，保证诱导分化为成熟的、功能活跃的脂肪细胞。

每瓶 PGM-2TM BulletKitTM 培养基都含有足够的分化培养基，可将 1000 万个前脂肪细胞 [10 个完整的 96 孔板] 完全分化为成熟的功能活跃的脂肪细胞。

PGMTM-2 培养基以 BulletKitTM 培养基（目录号 PT-8002）的形式提供，其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。

基础培养基（PGMTM-2 基础培养基；目录号 PT-8202）和必要的补充剂（PGMTM-2 SingleQuotTM 试剂盒；目录号 PT-9502）也可以单独购买。

Content & Storage

Content

1 x PGM^TM-2 Basal Medium (PT-8202)

1 x PGM^TM-2 SingleQuots^TM Supplement and Growth Factor Pack (PT-9502)

Related Products

PGM-2TM Preadipocyte Growth Medium-2 BulletKitTM

Human Subcutaneous Preadipocyte Cells PT-5020

HprAD – Human Subcutaneous Preadipocyte Cells PT-5001

PBM-2 Preadipocyte Basal Medium-2 PT-8202

PBM-2 前脂肪细胞基础培养基-2 PT-8202

PGM-2 Preadipocyte Growth Medium SingleQuots™- Supplements and Growth Factors PT-9502

PGM-2 前脂肪细胞生长培养基 SingleQuots™- 补充剂和生长因子 PT-9502

Trypsin/EDTA for Mesenchymal Stem Cells CC-3232

AdipoRed™ Assay Reagent, 20 mL PT-7009

hMSC – Human Mesenchymal Stem Cells PT-2501

hADSC Human Adipose-Derived Stem Cells PT-5006

DPSC – Human Dental Pulp Stem Cells PT-5025

Diseased Human Visceral Preadipocyte Cells – Diabetes Type I PT-5023

患病的人类内脏前脂肪细胞 – I 型糖尿病 PT-5023

Diseased Human Visceral Preadipocyte Cells – Diabetes Type II PT-5024

患病的人类内脏前脂肪细胞 – II 型糖尿病 PT-5024

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type I PT-5021

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II PT-5022

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007

糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5007

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008

糖尿病 I 型和 II 型脂肪干细胞 (ADSC) PT-5008

MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ PT-3001

MSCGM™ 间充质干细胞生长培养基 BulletKit™ PT-3001

lonza,PT-5020,人皮下前脂肪细胞,HprAD-人皮下前脂肪细胞,PT-5001

Catalog #: PT-5020

Cryopreserved ampule of Human Subcutaneous Preadipocytes containing ≥1 million cells

Product Overview

PoieticsTM 正常人前脂肪细胞 (HprAD) 是从正常（非糖尿病）供体的皮下或内脏脂肪中分离出来的。来自糖尿病供体的前脂肪细胞可作为单独的产品获得。相关的额外捐赠者信息，包括体重指数 (BMI) 可通过联系科学支持获得。

冷冻保存的 PoieticsTM 正常人前脂肪细胞 (HprAD) 保证每安瓿含有≥100 万或≥400 万（仅皮下）细胞，并且脂肪分化检测呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Recommended Media and Reagents

PGM^TM -2 Preadipocyte Growth Medium-2 BulletKit^TM Medium (provides both a proliferation medium and a differentiation medium guaranteed to induce differentiation into mature, functionally active adipocytes; Each bottle of the PGM-2^TM BulletKit^TM Medium contains enough differentiation medium to fully differentiate 10 million preadipocytes [10 complete 96-well plates] into mature, functionally active adipocytes).

PGMTM-2 培养基以 BulletKitTM 培养基（目录号 PT-8002）的形式提供，其中包括基础培养基和前脂肪细胞增殖和分化所需的补充剂。基础培养基（PGMTM-2 基础培养基；目录号 PT-8202）和必要的补充剂（PGMTM-2 SingleQuotTM 试剂盒；目录号 PT-9502）也可以单独购买。

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Preadipocytes containing ≥1 million cells

Related Products

HprAD – Human Subcutaneous Preadipocyte Cells PT-5001

HprAD – 人皮下前脂肪细胞 PT-5001

AdipoRed™ Assay Reagent, 20 mL PT-7009

Trypsin/EDTA for Mesenchymal Stem Cells CC-3232

PBM-2 Preadipocyte Basal Medium-2 PT-8202

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

Human Visceral Preadipocyte Cells PT-5005

hADSC Human Adipose-Derived Stem Cells PT-5006

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5007

Diabetic Type I & Type II Adipose Derived Stem Cells (ADSC) PT-5008

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type I PT-5021

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II PT-5022

Diseased Human Visceral Preadipocyte Cells – Diabetes Type II PT-5024

患病的人类内脏前脂肪细胞 – II 型糖尿病 PT-5024

PGM-2 Preadipocyte Growth Medium SingleQuots™- Supplements and Growth Factors PT-9502

PGM-2 前脂肪细胞生长培养基 SingleQuots™- 补充剂和生长因子 PT-9502

Diseased Human Visceral Preadipocyte Cells – Diabetes Type I PT-5023

患病的人类内脏前脂肪细胞 – I 型糖尿病 PT-5023

lonza,CC-2540B,支气管上皮细胞培养试剂盒,CC-3171,CC-4175

Catalog #: CC-2540B

完整的细胞培养试剂盒包含一个冷冻保存的支气管上皮细胞安瓿 (CC-2540) 和 BEGMTM 生长培养基 BulletKitTM (CC-3170)，用于培养支气管上皮细胞。

Product Overview

购买我们的支气管上皮细胞培养试剂盒并获得细胞和培养基的折扣价。

该试剂盒由冷冻保存的支气管上皮细胞安瓿（货号 CC-2540）和用于培养这些支气管上皮细胞的 BEGMTM BulletKitTM 生长培养基（货号 CC-3170）组成

Content & Storage

Content

1 x Cryopreserved ampule of Bronchial Epithelial Cells (CC-2540), ≥ 500,000 cells

1 x BEBM^TM Bronchial Epithelial Cell Growth Basal Medium (CC-3171), 500 mL

1 x BEGM^TM SingleQuots^TMSupplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL

Related Products

BEGM^TM Bronchial Epithelial SingleQuots^TM Kit CC-4175

BEGMTM支气管上皮SingleQuotsTM试剂盒CC-4175

BEBM™ Bronchial Epithelial Cell Growth Basal Medium CC-3171

BEBM™ 支气管上皮细胞生长基础培养基 CC-3171

lonza CC-2586,CC-2511,原代细胞和干细胞,NHEM-Ad-成人表皮黑色素细胞

Catalog #: CC-2586

NHEM-Ad, Epidermal Melanocytes, Adult

Overview

Product Overview

成人和新生儿人类表皮黑色素细胞 (NHEM) 是位于所有哺乳动物皮肤表皮层的色素生成细胞。黑色素细胞的功能是产生黑色素，黑色素是赋予皮肤颜色的色素。黑色素保护皮肤免受紫外线伤害。皮肤由2个主要层组成，表皮和真皮。表皮由多个角质形成细胞层组成。黑素细胞位于表皮的基底层。星状或树突状黑素细胞形态允许黑素细胞细胞体驻留在表皮的基底层，而树突与表皮上层的角质形成细胞结合。这种关联允许黑色素细胞将黑色素从细胞体通过树突转运到角质形成细胞中，从而导致皮肤着色。

黑素细胞研究有两个主要领域——化妆品和医学。化妆品研究中心专注于改变皮肤颜色的能力。医学研究通常集中在黑色素瘤以及各种皮肤病或相关疾病，如白化病和白癜风、激素治疗、甲状腺疾病、牛皮癣、烧伤和疤痕。

Our Cryopreserved Normal Human Epidermal Melanocytes are guaranteed through 8 population doublings, express Mel-5 and L-Dopa conversion to melanin. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Note: Melanocytes are extremely sensitive to over trypsinization. Use Lonza’s Trypsin/EDTA Solution (CC-5012) and dilute 1:1 with Versene^{^®}(17-711E) prior to use.

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products

NHEM-Ad – Human Epidermal Melanocytes, Adult

NHDF-Ad – Human Dermal Fibroblasts, Adult CC-2511

NHDF-Ad – 人皮肤成纤维细胞，成人 CC-2511

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543

HMVEC-dAd – 人真皮微血管内皮细胞 – 成人 CC-2543

NHEK-Ad – Human Epidermal Keratinocytes, Adult, Single Donor 00192627

NHEK-Ad – 人表皮角质形成细胞，成人，单一供体 00192627

lonza C2517AS,HUVEC-人脐静脉内皮细胞，单一供体，在 EGM™-2 中，预先筛选血管生成

Catalog #: C2517AS

HUVEC, single donor, in EGM^TM-2, Pre-screened for angiogenesis

Overview

Product Overview

Lonza’s Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed through 15* population doublings (*except HUVEC-XL^TM guaranteed through 5 PD), express CD31/105, von Williebrand Factor VIII, and are positive for acetylated low density lipoprotein uptake. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled version is typically utilized to reduce donor variability while single donor option allows researchers to assess donor-specific differences.

HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.

Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?

C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGM^TM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.
C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) – Isolated in EGM^TM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGM^TM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
CC-2935 (single donor) – Isolated in EGM^TM – PlusMedia: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGM^TM Media. No exogenous VEGF.

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis

RAFT™ 24-Well Small Kit, 3D Reagents & 24-Well Plate Absorbers – 12 Reactions 016-1R16

RAFT™ 24 孔小试剂盒、3D 试剂和 24 孔板吸收器 – 12 种反应 016-1R16

HAEC – Human Aortic Endothelial Cells CC-2535

HAEC – 人主动脉内皮细胞 CC-2535

HCAEC – Human Coronary Artery Endothelial Cells CC-2585

HCAEC – 人冠状动脉内皮细胞 CC-2585

lonza CC-4542,EGM™-Plus 内皮细胞生长培养基-加上 SingleQuot™ 试剂盒和生长补充剂

Catalog #: CC-4542

EGM^TM Plus Endothelial SingleQuot^TM Kit

Product Overview

EGMTM -Plus SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。这些是 EGMTM -Plus 内皮细胞生长培养基 BulletKitTM（目录号 CC-5035）的一部分，它由包装在一起的基础培养基和补充剂组成。

从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。
The following Endothelial Cell Growth Medium BulletKits^TM are available:

EGM^TM-2 BulletKit^TM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
EGM^TM BulletKit^TM – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
EGM^TM-Plus BulletKit^TM – Enhanced version of EGM^TM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGM^TM Medium.
EGM^TM-2MV BulletKit^TM – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.

Content & Storage

Content

1 x Bottle FBS 10.00 mL
1 x Vial Hydrocortisone 0.50 mL
1 x Bottle L-Glutamine 25.00 mL
1 x Vial EnGS 1.00 mL
1 x Vial Ascorbic Acid 0.50 mL
1 x Vial rhEGF 0.50 mL
1 x Vial GA-1000 0.50 mL
1 x Vial Heparin 0.50 mL

Related Products

EGM™-Plus Endothelial Cell Growth Medium - Plus SingleQuot™ Kits and Growth Supplements

ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935

HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体

lonza CC-2556 HRE-人肾上皮细胞,REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

Catalog #: CC-2556

HRE, Renal Epithelial Cells

Overview

Product Overview

Lonza 的人肾细胞包括从对每种细胞类型特异的各种正常人肾组织中分离出来的细胞。人肾近端小管细胞从近端小管中分离出来，是药物转运蛋白研究的理想选择。人肾上皮细胞 (HRE) 由来自皮质和肾小球区域的细胞组成。人肾皮质细胞 (HRCE) 来自近端和远端小管。

选择大量在没有庆大霉素-两性霉素的情况下分离的肾近端小管上皮细胞是可用的。这些批次在强力霉素中分离。请联系您当地的客户代表购买这些批次。

肾上皮细胞表现出极化形态，这对其功能至关重要。它们重新吸收肾小球滤液中几乎所有的葡萄糖和氨基酸，同时允许其他没有营养价值的物质从尿液中排出。渗透调节和排泄等生理过程可以在体外用 HRE 进行研究。人肾上皮细胞也能够内化细菌，并且位于各种先天性、代谢和炎症性疾病的损伤部位附近。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products

InEpC – Human Intestinal Epithelial Cells CC-2931

InEpC – 人肠上皮细胞 CC-2931

H-RPE – Human Retinal Pigment Epithelial Cells 00194987

H-RPE – 人视网膜色素上皮细胞 00194987

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190

REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

lonza CC-4526,原代细胞和干细胞,FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™

Catalog #: CC-4526

含有成纤维细胞生长所需的 FBMTM 基础培养基 (CC-3131) 和 FGMTM-3 SingleQuot 补充剂 (CC-4525) 的培养系统

Product Overview

FGM^TM-3 Fibroblast Growth Medium-3 was developed specifically for culturing primary cardiac fibroblasts. The kit contains basal medium and supplements packaged together for convenience to culture fibroblasts. FGM^TM-3 Medium contains contains 10% serum.

FGMTM-3 Fibroblast Growth Medium-3 专为培养原代心脏成纤维细胞而开发。该试剂盒包含包装在一起的基础培养基和补充剂，以方便培养成纤维细胞。 FGMTM-3 培养基含有 10% 血清。

Content & Storage

Content

1 x FBM^TM Basal Medium (CC-3131), 500 mL

1 x FGM^TM-3 SingleQuots^TM Supplement Pack (CC-4525) containing:

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Gray Cap Vial with hFGF-B, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

1 x Bottle FBS, 50.00 mL

Related Products

FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™

NHCF-A – Human Atrial Cardiac Fibroblasts CC-2903

NHCF-A – 人心房心脏成纤维细胞 CC-2903

FBM™ Fibroblast Growth Basal Medium, 500 mL CC-3131

FBM™ 成纤维细胞生长基础培养基，500 mL CC-3131

NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904

NHCF-V – 人心室心脏成纤维细胞 CC-2904

FGM™3 Cardiac Fibroblast Growth Medium-3 SingleQuots™ Supplements and Growth Factors CC-4525

FGM™3 心脏成纤维细胞生长培养基-3 SingleQuots™ 补充剂和生长因子 CC-4525

lonza,lonza CC-3249,MGM™-4 黑色素细胞生长培养基-4 BulletKit™

Catalog #: CC-3249

含有黑色素细胞生长所需的 MBMTM-4 基础培养基 (CC-3250) 和 MGMTM-4 SingleQuotsTM 补充剂 (CC-4435) 的培养系统

Product Overview

MGMTM-4 黑色素细胞生长培养基-4 是推荐用于黑色素细胞在培养中最佳生长和存活的培养基。该介质以我们传统的 BulletKitTM 格式提供，可根据研究需要灵活地添加或省略组件。

ET-3 生长补充剂是成人黑色素细胞生长所需的额外生长因子，单独出售，以补充 MGMTM-4 BulletKitTM 的完整配方，专门设计用于生长成人黑色素细胞。除了限制培养中的其他细胞类型污染外，ET-3 还提供增强的生长和铺板优势。

Content & Storage

Content

1 x MBM^TM-4 Basal Medium (CC-3250), 500 mL

1 x MGM^TM-4 SingleQuots^TM Supplement Pack (CC-4435) containing:

1 x Natural Cap Vial with CaCl2, 1.00 mL
1 x Amber Vial with PMA, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 1.00 mL
1 x Lilac Cap Vial with Insulin, 1.00 mL
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Bottle FBS, 2.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL

Related Products

MGM™-4 Melanocyte Growth Medium-4 BulletKit™

MBM^TM-4 Melanocyte Growth Basal Medium-4 CC-3250

MBMTM-4 黑色素细胞生长基础培养基-4 CC-3250

MGM™- 4 Melanocyte Growth Medium-4 SingleQuots™ Supplements and Growth Factors CC-4435

MGM™- 4 黑色素细胞生长培养基-4 SingleQuots™ 补充剂和生长因子 CC-4435

lonza 1M-105,原代细胞和干细胞,未加工的人骨髓10 mL

Catalog #: 1M-105

Fresh Unprocessed Human Bone Marrow, 10 mL

Overview

Product Overview

We Find the Donor. You Find the Cure.

More Cells

所有用于研究的、未经处理的新鲜骨髓样本每毫升含有超过 1500 万个有核活细胞，为您提供更多细胞，物有所值。可以以 10 或 25 mL 的数量订购每个供体总共 100 mL。每个供体最多可订购 100 mL。请咨询大量新鲜未加工骨髓的定价。

Fresh Delivery

未经处理的骨髓样本永远不会被稀释，并在环境温度下运输，以便第二天交付，因此您的样本新鲜且有活力。未处理的骨髓订单在周一至周四发货，通常提前 1-2 周安排，具体取决于供体和数量要求。可提供国际运输，交货时间各不相同。

Relevant Results

样本表示对于确保您为目标人群收集相关结果非常重要。

Lonza 骨髓捐献者计划自 1994 年开始运作。20 多年来，它一直维持着庞大而多样的捐献者库，以实现相关研究成果。此外，我们还提供来自同一供体的全血和骨髓。

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis (CoA) is provided for each cell lot purchased

Content & Storage

Content

1 x Unprocessed Human Bone Marrow, 10 mL

Related Products

Unprocessed Human Bone Marrow, Fresh, 10 mL

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 100,000 2M-101

人骨髓 CD34+ 祖细胞，冷冻保存，100,000 2M-101

Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞，500 万个 2M-302

Human Cord Blood CD34+ Progenitor Cells, Cryopreserved, 500,000 cells 2C-101A

人脐带血 CD34+ 祖细胞，冷冻保存，500,000 个细胞 2C-101A

hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

lonza CC-5036,CC-5034,原代细胞和干细胞,EBM™- Plus 内皮细胞生长基础培养基-Plus

Catalog #: CC-5036

EBM^TM Plus Endothelial Basal Medium

Product Overview

EBM^TM Plus is an endothelial basal medium sold separately. This can be used with 1 kit of CC-4542 (endothelial cell growth medium SingleQuots^TM Supplements and Growth Factors for use with HUVEC cells). The full BulletKit^TM consisting of basal medium and supplements can be purchased together using the catalog number CC-5035. Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow Clonetics^TM Primary Cells.

EBMTM Plus 是一种内皮基础培养基，单独出售。这可以与 1 套 CC-4542（内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子用于 HUVEC 细胞）一起使用。由基础培养基和补充剂组成的完整 BulletKitTM 可以使用目录号 CC-5035 一起购买。从多种内皮细胞生长培养基中进行选择，以轻松培养原代内皮细胞。每种内皮细胞生长培养基都有其特殊的配方，并针对某些细胞类型进行了优化。每种类型的内皮细胞生长培养基在用于培养 CloneticsTM 原代细胞时都能保证发挥作用。

The following Endothelial Cell Growth Medium BulletKits^TM are available:

EGM^TM-2 BulletKit^TM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
EGM^TM^TM BulletKit^TM – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
EGM^TM-Plus BulletKit^TM – Enhanced version of EGM^TM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGM^TM Medium.
EGM^TM-2 MV BulletKit^TM – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
EGM^TM-MV BulletKit^TM – Bovine Microvascular Cells, contains 5% FBS and BBE. Does not contain exogenous VEGF.

Content & Storage

Content

1 x Bottle Basal Medium 475 mL

Related Products

EBM™- Plus Endothelial Cell Growth Basal Medium-Plus

ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935

HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体

lonza CC-4107,原代细胞和干细胞,hEGF-人表皮生长因子

Catalog #: CC-4107

hEGF, Human Epidermal Growth Factor, 3 µg/mL, 0.5 mL

Product Overview

表皮生长因子 (EGF) 是一种通过与其受体 EGFR 结合来刺激细胞生长、增殖和分化的生长因子。人 EGF 是一种 6045-Da 蛋白质，具有 53 个氨基酸残基和三个分子内二硫键 EGF 导致细胞增殖、分化和存活。

重组表皮生长因子，浓度为 3 µg/ml

Content & Storage

Content

1 x Vial Containing 3 ug/mL hEGF 0.5 mL

lonza CC-4124,SAGM™ 小气道上皮细胞生长培养基 SingleQuots™ 添加剂和生长因子

Catalog #: CC-4124

SAGM^TM Small Airway Epithelial SingleQuots^TM Kit

Product Overview

SAGMTM SingleQuotTM 试剂盒是单独出售的组件，不包括基础培养基。这些是完整 SAGMTM 小气道上皮细胞生长培养基 BulletKitTM（目录号 CC-3118）的一部分，该培养基由包装在一起的基础培养基和补充剂组成。 SAGMTM BulletKitTM

SAGMTM BulletKitTM 不含血清，经过优化可培养人小气道上皮细胞 (SAEC)。

Content & Storage

Content

1 x Orange Cap Vial with BPE 2.0 mL
1 x Lilac Cap Vial with Insulin 0.5 mL
1 x Natural Cap Vial with Hydrocortisone 0.5 mL
1 x Red Cap Vial with GA-1000 0.5 mL
1 x Amber Vial with Retinoic Acid 0.5 mL
1 x Bottle with Fatty Acid Free BSA 5 mL
1 x Natural Cap Vial with Transferrin 0.5 mL
1 x Amber Vial with Triiodothyronine 0.5 mL
1 x Amber Vial with Epinephrine 0.5 mL
1 x Green Cap Vial with hEGF 0.5 mL

Related Products

SAGM™ Small Airway Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors

HMEC – Human Mammary Epithelial Cells CC-2551

HMEC – 人乳腺上皮细胞 CC-2551

SAEC – Human Small Airway Epithelial Cells CC-2547

SAEC – 人类小气道上皮细胞 CC-2547

D-SAEC-As – Diseased Small Airway Epithelial Cells – Asthma CC-2932

D-SAEC-As – 患病的小气道上皮细胞 – 哮喘 CC-2932

D-SAEC-COPD – Diseased Small Airway Epithelial Cells – COPD CC-2934

D-SAEC-COPD – 患病小气道上皮细胞 – COPD CC-2934

D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis CC-2933

D-SAEC-CF – 患病小气道上皮细胞 – 囊性纤维化 CC-2933

SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit™ CC-3118

SAGM™ 小气道上皮细胞生长培养基 BulletKit™ CC-3118

Data

SAGM™ Small Airway Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors

Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media

数据由 Furukawa 等人提供。 Lonza 的 SAEC 细胞与原代正常乳腺上皮细胞、乳腺癌细胞系 MCF-7 和 MDA-MB-231 在 Lonza 的 SAGM™ BulletKit™ 生长培养基中共培养。乳腺癌细胞系在存在小气道上皮细胞的情况下表现出形态和增殖率的差异。上图显示了 MCF-7 细胞系的荧光（红色）覆盖的相差成像。 MCF-7 细胞系在 SAGM™ 培养基中以紧密簇的形式存活。当与 Lonza 的原代 SAEC 共培养时，MCF-7 细胞在保持聚集的同时表现出增强的生长。

lonza CC-3119,原代细胞和干细胞,SABM™ 小气道上皮细胞生长基础培养基

Catalog #: CC-3119

SABM^TM Small Airway Epithelial Basal Medium

Product Overview

SABMTM 是一种基础培养基，单独出售。这被用作完整 SAGMTM 小气道上皮细胞生长培养基 BulletKitTM（目录号 CC-3118）的一个组件，该培养基由包装在一起的基础和补充剂组成。 SAGMTM BulletKitTM 不含血清，经过优化可培养人小气道上皮细胞 (SAEC)。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products

SABM™ Small Airway Epithelial Cell Growth Basal Medium

HMEC – Human Mammary Epithelial Cells CC-2551

HMEC – 人乳腺上皮细胞 CC-2551

B-ALI™ Bronchial Air-Liquid Interface Medium BulletKit™ 00193514

B-ALI™ 支气管气液接口中号 BulletKit™ 00193514

SAEC – Human Small Airway Epithelial Cells CC-2547

D-SAEC-As – Diseased Small Airway Epithelial Cells – Asthma CC-2932

D-SAEC-As – 患病的小气道上皮细胞 – 哮喘 CC-2932

D-SAEC-COPD – Diseased Small Airway Epithelial Cells – COPD CC-2934

D-SAEC-COPD – 患病小气道上皮细胞 – COPD CC-2934

D-SAEC-CF – Diseased Small Airway Epithelial Cells – Cystic Fibrosis CC-2933

D-SAEC-CF – 患病小气道上皮细胞 – 囊性纤维化 CC-2933

SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit™ CC-3118

SAGM™ 小气道上皮细胞生长培养基 BulletKit™ CC-3118

Data

SABM™ Small Airway Epithelial Cell Growth Basal Medium

Co-culture Experiments with Lonza’s Small Airway Epithelial Cells and SAGM™ BulletKit™ Media

lonza 2W-501,原代细胞和干细胞,NK-人类自然杀伤细胞

Catalog #: 2W-501

Crypreserved ampule of Peripheral Blood Human Natural Killer Cells (CD56 negative immunomagnetic selection) containing ≥ 5 million cells

Product Overview

自然杀伤 (NK) 细胞是免疫系统的淋巴细胞，作为先天性和适应性免疫反应的一部分，对宿主防御和免疫调节至关重要。它们对免疫治疗研究人员特别感兴趣，因为它们有望专门针对和破坏癌细胞和肿瘤，同时保持健康细胞完好无损。

Cells You Can Count On

自然杀伤细胞的传统特征是存在 CD56 细胞表面标志物且不存在 CD3。此外，CD16 对 NK 细胞功能很重要，因为 CD16 诱导细胞因子表达和细胞毒效应活性。

使用 CD56 标记的阳性 (2W-502) 或阴性 (2W-501) 免疫磁性选择分离 Lonza NK 细胞。基于 CD56+ 细胞的存在，纯度≥90%。阳性和阴性选择的 NK 细胞都保证表达 CD56 和 CD16。然而，NK 细胞的负免疫磁性分离导致 CD16+ 细胞的富集显着增加（通常 > 总细胞的 80%），而 CD16 的表达随阳性选择的 NK 细胞而变化。

Lonza NK 细胞保证表达 CD56 和 CD16，每瓶含有 ≥500 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Choices in Cell Type and Tissue Source

CD56和CD16的表达可以极大地影响细胞功能。此外，选择技术也会影响目标细胞的功能。这就是为什么 Lonza 提供阳性和阴性选择的 NK 细胞，并表征每批细胞中 CD56 和 CD16 的表达水平。

如果外周血衍生的自然杀伤细胞不是您想要的，我们还提供组织来源选择。脐带血 MNC 或骨髓 MNC 可以去除谱系标记表达细胞，以分离 NK 细胞。此外，脐带血 CD34+ 细胞或骨髓 CD34+ 细胞可分化为自然杀伤细胞。

Recommended Media and Reagents

LGM-3^TM Lymphocyte Growth Medium is recommended for optimal performance of NK cells. Additional cytokines may be necessary depending on application.

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood NK containing ≥ 5 million cells

Related Products

NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million

LGM-3^TM Lymphocyte Growth Medium-3 CC-3211

LGM-3TM 淋巴细胞生长培养基-3 CC-3211

NK – Human Natural Killer Cells 2W-502

NK – 人类自然杀伤细胞 2W-502

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

NHDC – Human Dendritic Cells CC-2701

Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 Million 2W-200

人外周血 CD4+ T 细胞，冷冻保存，1000 万个 2W-200

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 100,000 2M-101

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 300,000 2M-101A

人骨髓 CD34+ 祖细胞，冷冻保存，300,000 2M-101A

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 500,000 2M-101B

人骨髓 CD34+ 祖细胞，冷冻保存，500,000 2M-101B

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 1 Million 2M-101C

人骨髓 CD34+ 祖细胞，冷冻保存，100 万个 2M-101C

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 2 Million 2M-101D

人骨髓 CD34+ 祖细胞，冷冻保存，200 万个 2M-101D

Human Bone Marrow MNCs, Cryopreserved, 5 Million 2S-101D

人骨髓 MNC，冷冻保存，500 万个 2S-101D

Human Bone Marrow Mononuclear Cells (MNCs) (300 Million Cells) 2M-125E

人骨髓单个核细胞 (MNC)（3 亿个细胞）2M-125E

Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

未加工的人骨髓，新鲜，10 mL 1M-105

Unprocessed Human Bone Marrow, Fresh, 25 mL 1M-125

未加工的人骨髓，新鲜，25 mL 1M-125

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza 00195409,原代细胞和干细胞,RtEGM™ 视网膜色素上皮细胞生长培养基 BulletKit™

Catalog #: 00195409

Culture system containing RtEBM^TM Basal Medium (00195406) and RtEGM^TM SingleQuots^TM Supplements (00195407) required for growth of Retinal Pigment Epithelial Cells

含有视网膜色素上皮细胞生长所需的 RtEBMTM 基础培养基 (00195406) 和 RtEGMTM SingleQuotsTM 补充剂 (00195407) 的培养系统

Product Overview

RtEGMTM 视网膜色素上皮细胞生长培养基针对生长的人类 RPE 细胞进行了优化和性能测试。它含有2％的血清。

Applications

Age-related macular degeneration
Retinitis pigmentosa
Diabetic retinopathy
Photoreceptor degeneration
Gene expression
Cellular interactions

Content & Storage

Content

1 x RtEBM^TM Basal Medium (00195406), 200 mL

1 x RtEGM^TM SingleQuots^TM Supplement Pack (00195407) containing:

1 x Bottle L-Glutamine, 4.00 mL
1 x Gray Cap Vial with hFGF-B, 1.00 mL
1 x Bottle FBS, 4.00 mL
1 x Red Cap Vial with GA-1000, 0.20 mL

Related Products

RtEGM™ Retinal Pigment Epithelial Cell Growth Medium BulletKit™

H-RPE – Human Retinal Pigment Epithelial Cells 00194987

H-RPE – 人视网膜色素上皮细胞 00194987

RtEBM™ Retinal Epithelial Cell Basal Medium 00195406

RtEBM™ 视网膜上皮细胞基础培养基 00195406

RtEGM™ Retinal Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors 00195407

RtEGM™ 视网膜上皮细胞生长培养基 SingleQuots™ 添加剂和生长因子 00195407

lonza CC-2512B,原代细胞和干细胞,肺成纤维细胞培养试剂盒

Catalog #: CC-2512B

Lung Fibroblast Cell Culture Kit

肺成纤维细胞培养试剂盒

Product Overview

购买我们的肺成纤维细胞培养试剂盒并获得细胞和培养基的折扣价。该试剂盒包括一个冷冻保存的肺成纤维细胞安瓿（货号 CC-2512）和 FGMTM-2 BulletKitTM 成纤维细胞生长培养基（货号 CC- 第3132章

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial
1 x Bottle Basal Medium 500 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 10.00 mL

lonza CC-2935,HUVEC-EGM™-Plus 中不含 VEGF 的人脐静脉内皮细胞，单一供体

Catalog #: CC-2935

Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC) without VEGF, from a single donor, cultured in EGM^TM Plus and containing ≥ 500,000 cells

来自单一供体的不含 VEGF 的人脐静脉内皮细胞 (HUVEC) 的冷冻保存安瓿，在 EGMTM Plus 中培养并含有 ≥ 500,000 个细胞

Product Overview

HUVECs cultured in EGM^TM-Plus Growth Media offer faster proliferation rates while maintaining the same high quality characterization as HUVECs in EGM^TM Media

Lonza’s Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XL^TM guaranteed through 5 PD).

在 EGMTM-Plus 生长培养基中培养的 HUVEC 可提供更快的增殖速率，同时保持与 EGMTM 培养基中 HUVEC 相同的高质量表征

Lonza 的冷冻保存的 HUVEC 作为冷冻初级产品运输。它们保证表达 CD31/105 并通过 15* 种群倍增在培养中存活（* HUVEC-XLTM 除外，保证通过 5 PD）。

所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

HUVEC-XL 大小由 1000 万个细胞/安培组成，通过汇集供体实现。此选项旨在支持需要大量细胞的研究，同时仍为研究人员提供成本效益。

Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?

CC-2935 (single donor) – Isolated inEGM^TM – PlusMedia: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGM^TM Media. No exogenous VEGF.
CC-2517 (single donor), CC-2519 (pooled donor) – Both isolated in EGM^TM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) – Isolated in EGM^TM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
C2517AS (single donor), C2519AS (pooled donor) – Pre-screened HUVECs in EGM^TM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.

Benefits

Guaranteed through 15 population doublings
Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate of Analysis is provided for each cell lot purchased

Applications

Would healing
Angiogenesis
Tissue engineering
Inflammation
Oncology
Pharmacology
Vascular modeling
Transfection

Content & Storage

Content

1 x Cryopreserved ampule HUVEC containing ≥ 500,000 cells/vial

Related Products

HUVEC - Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus

RAFT™ 24-Well Small Kit, 3D Reagents & 24-Well Plate Absorbers – 12 Reactions 016-1R16

RAFT™ 24 孔小试剂盒、3D 试剂和 24 孔板吸收器 – 12 种反应 016-1R16

HAEC – Human Aortic Endothelial Cells CC-2535

HAEC – 人主动脉内皮细胞 CC-2535

HCAEC – Human Coronary Artery Endothelial Cells CC-2585

HCAEC – 人冠状动脉内皮细胞 CC-2585

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2 C2517A

HUVEC – EGM™-2 C2517A 中的人脐静脉内皮细胞，单一供体

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis C2517AS

HUVEC – 人脐静脉内皮细胞，单一供体，在 EGM™-2 中，预先筛选血管生成 C2517AS

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2 C2519A

HUVEC – 人脐静脉内皮细胞，混合，在 EGM™-2 C2519A 中

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis C2519AS

HUVEC – 人脐静脉内皮细胞，汇集在 EGM™-2 中，预先筛选血管生成 C2519AS

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517

HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519

HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中

HUVEC – Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus CC-2935

HUVEC – EGM™-Plus CC-2935 中不含 VEGF 的人脐静脉内皮细胞，单一供体

lonza CC-2555,原代细胞和干细胞,PrEC-人类前列腺上皮细胞

Catalog #: CC-2555

Cryopreserved ampule of Prostate Epithelial Cells (PrEC) containing ≥500,000 cells

含有≥500,000 个细胞的冷冻保存的前列腺上皮细胞 (PrEC) 安瓿

Product Overview

Prostate cells provide a glandular function in the body by generating fluid which serves several functions in reproduction. Lonza’s Human Prostate Cells are available from epithelial, smooth muscle, and stromal tissue. Each cell type has an optimized growth media for best results in culture.

Prostate Epithelial Cells (PrEC) are guaranteed through 15 population doublings and stain positive for cytokeratin (clone 8.13). Cryopreserved PrEC are shipped in first or second passage.

All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
前列腺细胞通过产生在生殖中具有多种功能的液体来提供体内的腺体功能。 Lonza 的人类前列腺细胞可从上皮、平滑肌和基质组织中获得。每种细胞类型都有优化的生长培养基，以获得最佳培养效果。

前列腺上皮细胞 (PrEC) 通过 15 次群体倍增得到保证，并且细胞角蛋白染色呈阳性（克隆 8.13）。冷冻保存的 PrEC 在第一次或第二次运输中运输。

Benefits

Guaranteed through 15 population doublings
Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Applications

Benign prostatic hyperplasia (BPH)
Prostitis
Prostate cancer
Procreation research
Drug discovery
Male prostate physiology

Content & Storage

Content

1 x Cryopreserved ampule PrEC containing ≥500,000 cells

Related Products

PrSMC – Human Prostate Smooth Muscle Cells CC-2587

PrSMC – 人前列腺平滑肌细胞 CC-2587

PrSC – Human Prostate Stromal Cells CC-2508

PrSC – 人类前列腺基质细胞 CC-2508

PrEGM™ Prostate Epithelial Cell Growth Medium BulletKit™ CC-3166

PrEGM™ 前列腺上皮细胞生长培养基 BulletKit™ CC-3166

P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2012

P2 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-2012

P2 Primary Cell 4D-Nucleofector™ X Kit L V4XP-2024

P2 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-2024

P2 Primary Cell 4D-Nucleofector™ X Kit S V4XP-2032

P2 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-2032

P2 Primary Cell 96-well Nucleofector™ Kit V4SP-2096

P2 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-2960

P2 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-2960

P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2002

P2 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-2002

P2 Primary Cell 384-well Nucleofector™ Kit V5SP-2010

P2 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-2010

lonza CC-3232,原代细胞和干细胞,用于间充质干细胞的胰蛋白酶/EDTA

Catalog #: CC-3232

Trypsin/EDTA for MSC, 100 mL

Product Overview

Trypsin/EDTA (catalog no. CC-3232): is Lonza’s recommended Trypsin/EDTA for all strongly adherent primary cells including normal human articular chondrocytes (NHAC-Kn), human bone marrow derived mesenchymal stem cells (hMSC), and dental pulp stem cells (DPSC).

胰蛋白酶/EDTA（目录号 CC-3232）：是 Lonza 推荐的用于所有强粘附原代细胞的胰蛋白酶/EDTA，包括正常人关节软骨细胞 (NHAC-Kn)、人骨髓间充质干细胞 (hMSC) 和牙髓干细胞细胞（DPSC）。

lonza,lonza HUCS10P,DonorPlexTM 混合供体人肝细胞，10 个供体，混合性别

Catalog #: HUCS10P

汇集 10 个供体、混合性别的人类肝细胞，具有多种 CYP450、UGT、SULT 和转运蛋白活性。药物代谢物鉴定、表征和周转动力学的理想选择。

Product Overview

对于 DMPK 研究而言，一致且可靠的汇集供体人肝细胞来源非常重要。通过我们新的专有算法，我们能够预测 DonorPlexTM 人肝细胞的产量、活力和功能表型，这反过来将支持您的 DMPK 中更一致的结果。每批汇集的 10 位供体、混合性别的人肝细胞的特征在于活产率，以及 CYP1A2、CYP2A6、CYP2B6、CYP2C9、CYP2C19、CYP2E1、CYP3A4、UGT、SULT、OAT1B1/3、OCT 和 NTCP 活性。

凭借我们大量的单供体人类肝细胞库存，并使用我们的专有算法来预测表型，我们还可以定制生产具有表型的混合供体肝细胞批次，以满足您的需求。

DonorPlex™ 肝细胞 – 悬浮合格肝细胞非常适合短期（<4 小时）体外药物清除试验。悬浮合格的肝细胞不适用于需要在粘附的单层中培养肝细胞的应用。

Benefits

Targeted pooling method for predictable metabolism
Precharacterized for 9 CYPs and 4 drug transporters
High viability and yields
Lot sizes < 2000 are possible

Applications

Suspension metabolism studies

Content & Storage

Content

Cryo Vial, >5 million viable cells

Related Products

DonorPlexTM Pooled Donor Human Hepatocytes, 10 Donor, Mixed Gender

Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞，冷冻保存，可电镀 HUCPG

Human Hepatocytes, Cryopreserved Single Donor , Suspension HUCSD

人肝细胞，冷冻保存的单一供体，悬浮 HUCSD

lonza,lonza CC-2516,HMVEC-dNeo-人真皮微血管内皮细胞，新生儿，混合

Catalog #: CC-2516

HMVEC-d Neo, Dermal Microvascular Endothelial, Neonatal, Pooled

Product Overview

真皮微血管内皮细胞包括从成人或新生儿包皮的皮肤组织内的小血管中分离出来的细胞。培养的细胞纯度≥90%，并专门富集真皮 LEC 和 BEC。来自新生儿或成人的真皮微血管细胞 (HMVEC-D) 是血液 (HMVEC-DB1) 和淋巴 (HMVEC-DLy) 内皮细胞的混合群体。

成体细胞用“广告”表示；新生细胞用“Neo”表示。汇集和单一捐助者都可用。通过联系我们的科学支持团队，可以获得更多的捐赠者特征。 CloneticsTM 内皮细胞和培养基方便易用，使研究人员能够专注于结果。 CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。

冷冻保存的细胞在第三代运输。冷冻保存的真皮微血管内皮细胞保证通过 15 次群体倍增（CC-2543、CC-2505 和 CC-2516）和 12 次群体倍增（CC-2810、CC-2811、CC-2812 和 CC-2813），表达 CD31/105 ，von Williebrand 因子 VIII，对乙酰化低密度脂蛋白摄取呈阳性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Related Products

HMVEC-dNeo – Human Dermal Microvascular Endothelial Cells, Neonatal, Pooled

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult CC-2543

HMVEC-dAd – 人真皮微血管内皮细胞 – 成人 CC-2543

NHEM-Neo – Human Epidermal Melanocytes, Neonatal CC-2504

EGM^TM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit^TM CC-3202

EGMTM -2 MV 微血管内皮细胞生长培养基-2 BulletKitTM CC-3202

P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5012

P5 Primary Cell 4D-Nucleofector™ X Kit L V4XP-5024

P5 Primary Cell 4D-Nucleofector™ X Kit S V4XP-5032

P5 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-5032

P5 Primary Cell 96-well Nucleofector™ Kit V4SP-5096

P5 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-5960

P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5002

P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5002

P5 Primary Cell 384-well Nucleofector™ Kit V5SP-5010

P5 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-5010

lonza CC-3216,原代细胞和干细胞,CGM™ 软骨细胞生长培养基 BulletKit™

Catalog #: CC-3216

CGM^TM Chondrocyte Growth BulletKit^TM

Product Overview

Articular cartilage is characterized by high wear resistance and poor regenerative qualities. It is responsible for much of the compressive resistance and load bearing qualities of the knee joint.

CGM^TM Chondrocyte Growth Medium is specially designed for expansion of chondrocytes. The BulletKit^TM is conveniently packaged with basal medium and supplements included for the growth of chondrocytes.
CDM^TM Chondrocyte Differentiation Medium is also available and sold separately for differentiation of human chondrocytes

Benefits

Applications:

Osteoarthritis
Cartilage failure
Cellular matrix linkage rupture
Chondrocyte protein synthesis inhibition
Chondrocyte apoptosis

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Bottle FBS 25.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Yellow Cap Vial with R3-IGF 1.00 mL
1 x Bottle bFGF 2.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Lilac Cap Vial with Insulin 1.00 mL

Related Products

CGM™ Chondrocyte Growth Medium BulletKit™

CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225

CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225

NHAC-kn – Human Knee Articular Chondrocytes CC-2550

NHAC-kn – 人膝关节软骨细胞 CC-2550

CBM™ Chondrocyte Growth Basal Medium CC-3217

CBM™ 软骨细胞生长基础培养基 CC-3217

CGM™ Chondrocyte Growth Medium SingleQuots™ Supplements and Growth Factors CC-4409

CGM™ 软骨细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4409

lonza CC-3125,EGM™-MV 微血管内皮细胞生长培养基 BulletKit™

Catalog #: CC-3125

EGM^TM-MV BulletKit^TM

Product Overview

Media products are supplied in several formats:

Basal Medium -– contains no growth factors, cytokines, or supplements
SingleQuots^TM Kit – growth factors, cytokines, and supplements
BulletKit^TM – basal medium and SingleQuots^TM Kit packaged together

The following Endothelial Cell Growth Medium BulletKits^TM are available:

EGM^TM BulletKit^TM – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.

EGM^TM-Plus BulletKit^TM – Enhanced version of EGM^TM Medium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGM^TM^TM Medium.

EGM^TM-2 BulletKit^TM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.

EGM^TM-MV BulletKit^TM – Bovine Microvascular Cells, contains 5% FBS and BBE. Does not contain exogenous VEGF.

EGM^TM-2 MV BulletKit^TM – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.

Related Products

EGM™-MV Microvascular Endothelial Cell Growth Medium BulletKit™

EBM™ Endothelial Cell Growth Basal Medium, 500 mL CC-3121

EBM™ 内皮细胞生长基础培养基，500 mL CC-3121

EGM^TM-MV Microvascular Endothelial Growth Medium SingleQuots^TM Kit CC-4143

EGMTM-MV 微血管内皮生长培养基 SingleQuotsTM 试剂盒 CC-4143

ReagentPack™ Subculture Reagents, 100 mL CC-5034

ReagentPack™ 继代培养试剂，100 mL CC-5034

lonza CC-7049,原代细胞和干细胞,HPdLF-人牙周膜成纤维细胞

Catalog #: CC-7049

HPdLF, Periodontal Ligament Fibroblasts

Product Overview

人牙周韧带成纤维细胞 (HPdLF) 从将臼齿固定到颌骨的韧带中分离出来。保证人牙周膜成纤维细胞通过 10 倍种群倍增和泛细胞角蛋白染色阴性。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

lonza 2M-302,原代细胞和干细胞,人骨髓基质细胞，500 万

Catalog #: 2M-302

Cryopreserved ampule of Human Bone Marrow Stromal Cells containing ≥5 million cells

Product Overview

当骨髓细胞在特定条件下进行培养时，会形成粘附细胞的基质层。基质层由许多细胞类型组成，包括成纤维细胞、间充质干细胞、脂肪细胞、内皮细胞和巨噬细胞。这些基质细胞充当造血祖细胞和干细胞的饲养层，允许祖细胞和干细胞在不添加外源细胞因子的情况下在这些培养物中继续增殖和分化数周。

这种长期培养系统使研究人员能够研究骨髓微环境的体外模型，包括细胞间相互作用、粘附分子和细胞因子分泌。这些和许多其他因素允许对血细胞产生、祖细胞定型和分化、干细胞更新和 LTC-IC 测定进行严格调节。仅提供非辐照。

并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

推荐培养基：来自 StemCell Technologies, Inc. 的 MyeloCultTM H5100。

骨髓基质细胞经常与骨髓间充质干细胞混淆。如果您需要具有自我更新能力和分化多个谱系（包括成骨谱系、脂肪生成谱系和软骨生成谱系）的纯骨髓衍生多能干细胞群，请访问骨髓间充质干细胞页面。

只有在专门使用适当的培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis (CoA) is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Human Bone Marrow Non-Irradiated Stromal Cells containing ≥ 5 million cells

lonza CC-3129,EBM™ 内皮细胞生长基础培养基500 mL

Catalog #: CC-3129

Basal media required for growth of Endothelial cells, Phenol Red Free, 500 mL (supplements and growth factors sold separately)

内皮细胞生长所需的基础培养基，无酚红，500 mL（补充剂和生长因子单独出售）

Product Overview

EBMTM Phenol Red Free 是一种内皮基础培养基，单独出售。这可以与 1 套 CC-4133（内皮细胞生长培养基 SingleQuotsTM 补充剂和生长因子用于某些 HUVEC 细胞）一起使用。

The following Endothelial Cell Growth Medium BulletKits^TM are available:

- EGM^TM BulletKit^TM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519, contains 2% FBS and BBE. Does not contain exogenous VEGF.
- EGM^TM-Plus BulletKit^TM (CC-5035) – Enhanced version of EGM^TMMedium. Optimized for HUVECs cat. no. CC-2935. Contains 2% FBS and BBE. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGM^TMMedium.
- EGM^TM-2 BulletKit^TM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGM^TM-2 MV BulletKit^TM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.

Content & Storage

Content

1 x Bottle EBM^TM Phenol Free Basal Medium, 500 mL

EBM™ Endothelial Cell Growth Basal Medium, Phenol Red Free, 500 mL

EGM^TM Endothelial Cell Growth Medium SingleQuots^TM Kit CC-4133

EGMTM 内皮细胞生长培养基 SingleQuotsTM 试剂盒 CC-4133

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™ CC-2517

HUVEC – EGM™ CC-2517 中的人脐静脉内皮细胞，单一供体

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™ CC-2519

HUVEC – 人脐静脉内皮细胞，汇集，在 EGM™ CC-2519 中

lonza R-CX-500,原代细胞和干细胞,大鼠脑皮层神经元

Catalog #: R-CX-500

Cryopreserved ampule of Rat Brain Cortex Neurons containing ≥4 million cells

Product Overview

即用型 CloneticsTM 大鼠脑皮层来自大鼠 (E18, 19) 脑的神经元是通过标准化方法制备的高质量原代胚胎脑神经元细胞（包括神经胶质细胞）的细胞悬液，可立即进行培养。

每瓶皮质细胞含有大约 400 万个活细胞。细胞将使用推荐的接种密度和培养基接种到 24 孔板的 10 个或更多孔和 96 孔板的 50 个或更多孔中。接种后的最初几天会发生细胞死亡，并观察到碎片 . 这很正常。培养约 4 天后，细胞将形成神经突网络，到第 7 天，碎片将最少。

Recommended Medium: PNGM^TM BulletKit^TM(CC-4461)

Benefits

Ready-To-Use
No tissue dissection or animal handling required
Increased data reproducibility
Characteristic morphology and staining tests confirm tissue origin and cell type
Negative for mycoplasma, bacteria, yeast and fungi
Certificate of Analysis (CoA) is available for every batch

Applications

Neurogenesis
Neurotransmitter Function
Gene Expression
Signaling Pathways
Electrophysiology
Neurotoxicity
Drug/Compound Screening
Advanced Cell Culture Models

Content & Storage

Content

1 x Cryopreserved ampule of Rat Cortex Neurons containing ≥ 4,000,000 cells

Related Products

PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

Rat Cerebellar Granular Neurons R-CB-503

大鼠小脑颗粒神经元 R-CB-503

Rat Brain Striatum Neurons R-CP-502

Rat Neonatal Dorsal Root Ganglion (DRG) Neurons R-DRG-505

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons R-EDRG-515

大鼠胚胎背根神经节 (eDRG) 神经元 R-EDRG-515

Rat Brain Hippocampus Neurons R-HI-501

Rat Brain Hypothalamus Neurons R-HTH-507

大鼠脑下丘脑神经元 R-HTH-507

P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 Primary Cell 4D-Nucleofector^® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

lonza CC-2576,原代细胞和干细胞,BSMC-人支气管平滑肌细胞

Catalog #: CC-2576

Cryopreserved ampule of Bronchial Smooth Muscle Cells (BSMC) containing ≥ 500,000 cells

Product Overview

人支气管平滑肌细胞 (BMSC) 是从正常组织的主要支气管中分离出来的。细胞在第 2 代中运输。

患病的人支气管平滑肌细胞 (D-BSMC) 也可从被诊断患有哮喘、COPD 和囊性纤维化的捐赠者处获得。这些细胞的详细捐赠者信息可以通过联系我们的科学支持团队获得。

冷冻保存的 CloneticsTM 人支气管平滑肌细胞通过 15 次细胞倍增得到保证，α 平滑肌肌动蛋白染色呈阳性，von Willebrand 因子 VIII 呈阴性。所有细胞的支原体、细菌、酵母和真菌检测均为阴性。HIV-1、乙型肝炎和并非所有供体和/或细胞批次都检测到丙型肝炎。为购买的每个细胞批次提供分析证书。

Benefits

All cells test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis provided for every cell lot

Content & Storage

Content

1 x Cryopreserved ampule BSMC containing ≥ 500,000 cells/vial

Related Products

BSMC – Human Bronchial Smooth Muscle Cells

SAEC – Small Airway Epithelial Cells for S-ALI^TM Small Airway Air-Liquid-Interface Medium CC-2547S

SAEC – 用于 S-ALITM 小气道气液界面培养基 CC-2547S 的小气道上皮细胞

SAEC – Human Small Airway Epithelial Cells CC-2547

SAEC – 人类小气道上皮细胞 CC-2547

HMVEC-L – Human Lung Microvascular Endothelial Cells CC-2527

HMVEC-L – 人肺微血管内皮细胞 CC-2527

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1024

P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032

P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

lonza,CC-3207,OGM™ 成骨细胞生长培养基 BulletKit™

Catalog #: CC-3207

OGM^TM Osteoblasts BulletKit^TM

Product Overview

CloneticsTM OGMTM 成骨细胞生长培养基专为人类成骨细胞的生长和扩增而配制。该试剂盒含有 10% 的血清。该试剂盒包装方便，包括基础培养基和生长补充剂，用于成骨细胞的完全培养

OGMTM 分化补充剂也可单独购买和销售（目录号 CC-4194）。这些推荐用于分化测定。

Benefits

Applications:

In vitro bone models
Mineralization
Osteoporosis
Gene expression
Hormone treatments
Osteopenia
Paget’s Disease

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Bottle FBS 50.00 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid 0.50 mL

Related Products

OGM™ Osteoblast Growth Medium BulletKit™

NHOst – Human Osteoblasts CC-2538

NHOst – 人类成骨细胞 CC-2538

OBM™ Osteoblast Growth and Differentiation Basal Medium CC-3208

OBM™ 成骨细胞生长和分化基础培养基 CC-3208

OGM™ Osteoblast Growth Medium SingleQuots™ Supplements and Growth Factors CC-4193

OGM™ 成骨细胞生长培养基 SingleQuots™ 补充剂和生长因子 CC-4193

OGM™ Osteoblast Differentiation Medium SingleQuots™ Supplements and Growth Factors CC-4194

OGM™ 成骨细胞分化培养基 SingleQuots™ 补充剂和生长因子 CC-4194

lonza HUCSD,原代细胞和干细胞,人肝细胞，冷冻保存的单一供体，悬浮液

Catalog #: HUCSD

冷冻保存的单一供体人肝细胞的特点是在短期悬浮代谢测定中具有高活力和一般的 I 期和 II 期代谢活性。每个安瓿包含 > 500 万个细胞。

Product Overview

冷冻保存的单一供体人肝细胞的特点是在短期悬浮代谢测定中具有高活力和一般的 I 期和 II 期代谢活性。悬浮合格的肝细胞非常适合短期（<4 小时）体外药物清除试验。悬浮合格的肝细胞不适合需要在粘附的单层中培养肝细胞的应用。

Benefits

Precharacterized for general metabolism
Certificate of Analysis (CoA) provided for every cell lot

Applications

Suspension metabolism studies

Content & Storage

Content

1 x Cryopreserved ampule Human Hepatocytes Suspension, Single Donor, containing >5 million viable cells

Related Products

Human Hepatocytes, Cryopreserved Single Donor , Suspension

Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI

人肝细胞，冷冻保存，可电镀和相互作用合格的 HUCPI

Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞，冷冻保存，可电镀 HUCPG

lonza,原代细胞和干细胞,FGM™ 成纤维细胞生长培养基 BulletKit™

Catalog #: CC-3130

停产通知：该产品即将停产。当前库存将被出售，直到库存耗尽。请使用产品 CC-3132 作为替代品。

用于人成纤维细胞培养的成纤维细胞生长培养基 BulletKitTM

Product Overview

FGM^TM Fibroblast Growth Medium was developed to support growth of fibroblasts.

Content & Storage

Content

1 x FBMTM Basal Medium (CC-3131), 500 mL

1 x FGM SingleQuotsTM Supplement Pack (CC-4134) containing:

1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Gray Cap Vial with hFGF-B, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL

lonza,lonza HUCNP,lonza HUCLS,原代细胞和干细胞,人肝混合非实质细胞

Catalog #: HUCNP

Human cryopreserved liver mixed NPCs cells

人冷冻保存的肝脏混合 NPCs 细胞

Product Overview

Human Cryopreserve Liver Mixed NPCs are a native mixture of Non-parenchymal cells from human liver. These are supplied in > 1million cells/vial and can have a varying amount of the different non-parenchymal cells.

This product is most suitable for liver cell co-culture applications

人冷冻保存肝脏混合 NPC 是来自人肝脏的非实质细胞的天然混合物。这些以 > 100 万个细胞/小瓶的形式提供，并且可以具有不同数量的不同非实质细胞。

本产品最适合肝细胞共培养应用

Benefits

Native cell ratios,
Never passaged for close to in vivo function

Applications

ADMETox, Liver Disease, NASH

Content & Storage

Content

Cryo Vial

Related Products

Liver Mixed Non-parenchymal Cells, Human

Stellate cells, human cryopreserved, passage 1 HUCLS

星状细胞，人类冷冻保存，第 1 代 HUCLS

lonza,lonza 2M-125C,原代细胞和干细胞,人骨髓单个核细胞 MNC

货号: 2M-125C

Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing ≥25 million cells

含有≥2500万个细胞的人骨髓单个核细胞的冷冻保存安瓿

Product Overview

Bone marrow mononuclear cells (common abbreviations: BMNC, BM MNC, and BM-MNC) are a mixed population of single nucleus cells including monocytes, lymphocytes, and hematopoietic stem and progenitor cells.

骨髓单个核细胞（常用缩写：BMNC、BM MNC 和 BM-MNC）是单核细胞的混合群体，包括单核细胞、淋巴细胞以及造血干细胞和祖细胞。

BMNCs You Can Count on to Perform

Cryopreserved Lonza Bone Marrow Mononuclear Cells are isolated from whole bone marrow via density gradient separation. Isolating mononuclear cells eliminates erythrocytes and graulocytes, producing a more stable cell product. Lonza BMNCs are available fresh or frozen from a large and varied donor pool. They are guaranteed to contain either ≥5, 25, 100, 200 and 300 million viable cells per ampoule, depending upon product purchased. Fresh cells are suspended in HBSS with 5 mM EDTA and 0.5% BSA and are available in a variety of cells per vial packaging sizes. Fresh BMNC orders require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.

通过密度梯度分离从全骨髓中分离冷冻保存的龙沙骨髓单核细胞。分离单核细胞消除红细胞和粒细胞，产生更稳定的细胞产物。 Lonza BMNCs 可从大量多样的供体库中获得新鲜或冷冻。根据购买的产品，它们保证每个安瓿含有≥5、25、100、200 和 3 亿个活细胞。新鲜细胞悬浮在含有 5 mM EDTA 和 0.5% BSA 的 HBSS 中，每瓶包装尺寸有多种细胞可供选择。新鲜的 BMNC 订单要求每个捐赠者至少订购两瓶。有关新鲜细胞的尺寸和价格信息，请联系客户服务。

Choices in Cell Type and Tissue Source

Lonza offers choices in cell type and tissue source so you can choose the right product for your research and budget needs. Depending upon your ultimate cell of choice, you can choose from unprocessed bone marrow, bone marrow MNCs, bone marrow CD34+ cells, bone marrow stromal cells (for use as feeder cells), or bone marrow mesenchymal stem cells. In addition, if bone marrow is not your preferred tissue source, we also offer cord blood derived cells.

Lonza 提供细胞类型和组织来源的选择，因此您可以选择适合您的研究和预算需求的产品。根据您选择的最终细胞，您可以选择未加工的骨髓、骨髓 MNC、骨髓 CD34+ 细胞、骨髓基质细胞（用作饲养细胞）或骨髓间充质干细胞。此外，如果骨髓不是您首选的组织来源，我们还提供脐带血来源的细胞。

Supported by an Optimized Culture System

HPGM^TM Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application). Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.

建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能（根据应用，可能需要额外的细胞因子）。含有血清的替代培养基也是可以接受的。有关含血清培养基的建议，请联系科学支持。

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased
支原体、细菌、酵母菌和真菌检测呈阴性
并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
为购买的每个细胞批次提供分析证书

Content & Storage

Content

1 x Cryopreserved ampule BMNCs containing ≥ 25 million cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Related Products

Human Bone Marrow Mononuclear Cells MNCs, Cryopreserved, 25 Million Cells

Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

未加工的人骨髓，新鲜，10 mL 1M-105

Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞，500 万个 2M-302

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza,lonza 00195769,KGM™ Gold 角质形成细胞生长培养基 BulletKit™

Catalog #: 00195769

KGM^TM Gold Keratinocyte BulletKit^TM, Calcium Free

KGMTM Gold Keratinocyte BulletKitTM，无钙

Product Overview

A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures.

KGM-Gold^TM Calcium Free Keratinocyte Growth Medium is designed to support both clonal growth and high density keratinocyte proliferation. The medium supports superior growth and morphology of keratinocytes.

Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings.

一种新的稳健且多功能的无血清培养基配方，适用于高密度和低密度 NHEK-Normal 人类表皮角质形成细胞培养物。

KGM-GoldTM 无钙角质形成细胞生长培养基旨在支持克隆生长和高密度角质形成细胞增殖。该培养基支持角质形成细胞的卓越生长和形态。

此外，Lonza 的质量控制要求角质形成细胞通过严格的形态学标准，规定最小存在扩大和空泡细胞，使研究人员能够将 Lonza 角质形成细胞用于≥15 人口倍增。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.25 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – German

SDS – Italian

SDS – Dutch

SDS – French

SDS – Spanish

SDS – English

Related Products

KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free

KBM™ Gold Keratinocyte Growth Basal Medium, Calcium and Phenol Red Free 00195130

KBM™ Gold 角质形成细胞生长基础培养基，不含钙和酚红 00195130

KGM™ Gold Keratinocyte Growth Medium SingleQuots™ Supplements and Growth Factors 00192152

KGM™ Gold 角质形成细胞生长培养基 SingleQuots™ 补充剂和生长因子 00192152

Data

KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free

Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.

lonza,lonza CC-3108,KGM™-2 角质形成细胞生长培养基-2 BulletKit™

货号: CC-3108

KGM^TM 2 Keratinocyte BulletKit^TM, Calcium Free

Overview

Product Overview

KGM-2 Bulletkit^TMis also sold as separate basal medium and supplements

Keratinocyte Growth Medium-2 has been optimized for the proliferation of keratinocytes in a serum-free and calcium-free environment and typically provides higher growth rates than KGM^TM Medium.
Clonetics^TM Media Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems.

KGM-2 BulletkitTM 也作为单独的基础培养基和补充剂出售

角质形成细胞生长培养基 2 已针对角质形成细胞在无血清和无钙环境中的增殖进行了优化，通常比 KGMTM 培养基提供更高的生长速率。
CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。媒体系统已针对各种细胞系统专门开发和优化。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL

Instructions, SDS, CoA

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza,lonza 2W-502,lonza CC-3211,原代细胞和干细胞,NK-人类自然杀伤细胞

货号: 2W-502

Human Natural Killer Cells pos selection, ≥5 million cells

人自然杀伤细胞 pos 选择，≥500 万个细胞

Product Overview

Natural Killer (NK) cells are lymphocytes of the immune system that are critical in host defense and immune regulation as part of both the innate and adaptive immune response. They are of particular interest to immunotherapy researchers as they hold the promise to specifically target and destroy cancer cells and tumors while leaving healthy cells intact. Cells You Can Count on to Perform Natural Killer cells are traditionally characterized by the presence of the CD56 cell surface marker and absence of CD3. In addition, CD16 is important for NK cell function because CD16 induces cytokine expression and cytotoxic effector activity. Lonza NK cells are isolated using positive (2W-502) or negative (2W-501) immunomagnetic selection for the CD56 marker. Purity is ≥90% based on the presence of CD56+ cells. Both positively and negatively selected NK cells are guaranteed to express CD56 and CD16. However, negative immunomagnetic separation of NK cells results in significantly greater enrichment of CD16+ cells (typically > 80% of total cells), whereas expression of CD16 is variable with the positively selected NK cells. Lonza NK cells are guaranteed to express CD56 and CD16, and contain ≥5 million viable cells per vial. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Choices in Cell Type and Tissue Source Expression of CD56 and CD16 can greatly influence cell function. In addition, selection techniques can also influence function of your target cells. That’s why Lonza offers both positively and negatively selected NK cells and characterizes the expression levels of both CD56 and CD16 in every lot of cells. We also offer choices in tissue source if peripheral blood derived natural killer cells aren’t what you’re looking for. Cord blood MNCs or bone marrow MNCs can be depleted of lineage marker expressing cells in order to isolate NK cells. In addition, cord blood CD34+ cells or bone marrow CD34+ cells can be differentiated into natural killer cells. Supported by Lonza Medium LGM-3^TM Lymphocyte Growth Medium is recommended for optimal performance of NK cells. Additional cytokines may be necessary depending on application. Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

自然杀伤 (NK) 细胞是免疫系统的淋巴细胞，作为先天性和适应性免疫反应的一部分，对宿主防御和免疫调节至关重要。它们对免疫治疗研究人员特别感兴趣，因为它们有望专门针对和破坏癌细胞和肿瘤，同时保持健康细胞完好无损。您可以依靠的细胞来执行自然杀伤细胞的传统特征是存在 CD56 细胞表面标志物且不存在 CD3。此外，CD16 对 NK 细胞功能很重要，因为 CD16 诱导细胞因子表达和细胞毒效应活性。使用 CD56 标记的阳性 (2W-502) 或阴性 (2W-501) 免疫磁性选择分离 Lonza NK 细胞。基于 CD56+ 细胞的存在，纯度≥90%。阳性和阴性选择的 NK 细胞都保证表达 CD56 和 CD16。然而，NK 细胞的负免疫磁性分离导致 CD16+ 细胞的富集显着增加（通常 > 总细胞的 80%），而 CD16 的表达随阳性选择的 NK 细胞而变化。 Lonza NK 细胞保证表达 CD56 和 CD16，每瓶含有 ≥500 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。 CD56和CD16的细胞类型和组织来源表达的选择可以极大地影响细胞功能。此外，选择技术也会影响目标细胞的功能。这就是为什么 Lonza 提供阳性和阴性选择的 NK 细胞，并表征每批细胞中 CD56 和 CD16 的表达水平。如果外周血衍生的自然杀伤细胞不是您想要的，我们还提供组织来源选择。脐带血 MNC 或骨髓 MNC 可以去除谱系标记表达细胞，以分离 NK 细胞。此外，脐带血 CD34+ 细胞或骨髓 CD34+ 细胞可分化为自然杀伤细胞。由 Lonza 培养基支持 LGM-3TM 淋巴细胞生长培养基推荐用于 NK 细胞的最佳性能。根据应用，可能需要额外的细胞因子。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Content & Storage

Content

5 million cells/vial

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

LGM-3^TM Lymphocyte Growth Medium-3 CC-3211

LGM-3TM 淋巴细胞生长培养基-3 CC-3211

lonza,lonza 2W-400A,CC-2701,原代细胞和干细胞,人外周血 CD14+ 单核细胞

Catalog #: 2W-400A

Cryopreserved ampule of Human Peripheral Blood CD14+ Monocytes, positive selection, containing ≥40 million cells

人外周血 CD14+ 单核细胞冻存安瓿，阳性选择，含有≥4000 万个细胞

Product Overview

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the total mononuclear cell population. Monocytes are characterized by their phenotypic expression of CD14, and thus, are commonly called CD14+ monocytes. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability.

单核细胞存在于循环外周血中，占总单核细胞群的 10-20%。单核细胞的特征在于它们的 CD14 表型表达，因此，通常称为 CD14+ 单核细胞。它们作为循环单核细胞和分化成组织巨噬细胞在宿主防御中发挥重要作用，并且可以分化成具有强大抗原呈递能力的树突状细胞。

Cells You Can Count on to Perform

Lonza Human Peripheral Blood CD14+ Monocytes are isolated from peripheral blood of screened, healthy, untreated (non-mobilized peripheral blood) donors. Briefly, mononuclear cells (MNCs) are isolated from apheresis derived peripheral blood. The MNC population is then further purified via positive immunomagnetic selection directed against CD14. Cryopreserved CD14+ Monocytes are guaranteed to express CD14 and will contain either ≥10, 20, or 40 million viable cells per ampoule depending upon the product ordered. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Lonza Human Peripheral Blood CD14+ 单核细胞是从经过筛选的、健康的、未经治疗的（未动员的外周血）供体的外周血中分离出来的。简而言之，单核细胞 (MNC) 是从单采术衍生的外周血中分离出来的。然后通过针对 CD14 的阳性免疫磁性选择进一步纯化 MNC 群体。冷冻保存的 CD14+ 单核细胞保证表达 CD14，并且根据订购的产品，每个安瓿将包含 ≥10、20 或 4000 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Choices in Cell Type and Tissue Source

Hematopoietic and immune research requires a variety of donors and cell types. That’s why Lonza offers many different peripheral blood cell types from a variety of donors, including PBMCs and dendritic cells, among others. In addition, if you are interested in exploring the differences between monocytes in different maturation states or from different tissues, we offer bone marrow mononuclear cells.

造血和免疫研究需要多种供体和细胞类型。这就是为什么 Lonza 提供来自各种供体的多种不同外周血细胞类型，包括 PBMC 和树突状细胞等。此外，如果您有兴趣探索不同成熟状态或来自不同组织的单核细胞之间的差异，我们提供骨髓单核细胞。

Supported by Lonza Media

The recommended medium varies by application (see below). Please contact Scientific Support for additional culturing recommendations. Media recommendations for macrophage and dendritic cell differentiation are recommendations only.

Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
Dendritic Cells: LGM-3^TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.

Lonza CD14+ 单核细胞没有针对巨噬细胞或树突状细胞分化进行常规质量控制测试，并且细胞保修不保证分化。产生分化可能需要其他因素，包括细胞外基质或培养条件。

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 40 million cells

Instructions, SDS, CoA

Related Products

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million

NHDC – Human Dendritic Cells CC-2701

NHDC – 人类树突状细胞 CC-2701

P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-3012

P3 Primary Cell 4D-Nucleofector^® X Kit L V4XP-3024

P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-3096

P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

Data

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 40 million

Human CD14+ Monocytes

Human CD14+ monocytes differentiating to macrophages

lonza,lonza CC-2554,原代细胞和干细胞,HRCE – 人肾皮质上皮细胞

货号: CC-2554

冷冻保存的肾皮质上皮细胞 (HRCE) 安瓿，含有 ≥500,000 个细胞，Cryopreserved ampule of Renal Cortical Epithelial Cells (HRCE) containing ≥500,000 cells

Product Overview

Lonza’s Human Renal Cells include cells isolated from various normal human kidney tissue specific to each cell type. Human Renal Proximal Tubule Cells are isolated from the proximal tubule and are ideal for drug transporter studies. Human Renal Epithelial Cells (HRE) are comprised of cells from the cortex and glomerular region. Human Renal Cortical Cells (HRCE) are from proximal and distal tubules. Human Mesangial Cells (HMC) are isolated from the renal glomerulus and modified smooth muscle cells between capillaries.

Renal epithelial cells display a polarized morphology, which is essential for their function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. Physiological processes like osmoregulation and excretion can be studied with renal epithelial cells in vitro. Human renal epithelial cells are also capable of internalizing bacteria and are located near injury sites in a variety of congenital, metabolic and inflammatory diseases.

Lonza 的人肾细胞包括从对每种细胞类型特异的各种正常人肾组织中分离出来的细胞。人肾近端小管细胞从近端小管中分离出来，是药物转运蛋白研究的理想选择。人肾上皮细胞 (HRE) 由来自皮质和肾小球区域的细胞组成。人肾皮质细胞 (HRCE) 来自近端和远端小管。人系膜细胞 (HMC) 从肾小球和毛细血管之间的修饰平滑肌细胞中分离出来。

肾上皮细胞表现出极化形态，这对其功能至关重要。它们重新吸收肾小球滤液中几乎所有的葡萄糖和氨基酸，同时允许其他没有营养价值的物质从尿液中排出。可以在体外用肾上皮细胞研究渗透调节和排泄等生理过程。人肾上皮细胞也能够内化细菌，并且位于各种先天性、代谢和炎症性疾病的损伤部位附近。

Benefits

Guaranteed through 15 population doublings
Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased
通过 15 次人口倍增保证
支原体、细菌、酵母菌和真菌检测呈阴性
并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
为购买的每个细胞批次提供分析证书

Content & Storage

Content

1 x Cryopreserved ampule of HRCE containing ≥500,000 cells

Instructions, SDS, CoA

Related Products

HRCE – Human Renal Cortical Epithelial Cells

H-RPE – Human Retinal Pigment Epithelial Cells 00194987

H-RPE – 人视网膜色素上皮细胞 00194987

InEpC – Human Intestinal Epithelial Cells CC-2931

InEpC – 人肠上皮细胞 CC-2931

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

REGM™ Renal Epithelial Cell Growth Medium BulletKit™ CC-3190

REGM™ 肾上皮细胞生长培养基 BulletKit™ CC-3190

lonza,lonza MBCP01,原代细胞和干细胞,小鼠 C57BL/6 肝细胞

Catalog #: MBCP01

Cryopreserved ampule of C57BL/6 Mouse Hepatocytes, in a plateable format, containing > 5 million cells

冷冻保存的 C57BL/6 小鼠肝细胞安瓿，可电镀，含有 > 500 万个细胞

Product Overview

Cryopreserved C57BL/6 Mouse Hepatocytes, Plateable are specifically pre tested for metabolic activity of general Phase I and Phase II metabolism in suspension.

They are tested for healthy morphology lasting 72 hours in monlayer sandwich culture.

冷冻保存的 C57BL/6 小鼠肝细胞，可铺板专门针对悬浮液中一般 I 期和 II 期代谢的代谢活性进行了预测试。

它们在单层夹层培养中经过 72 小时的健康形态测试。

Applications

Drug metabolism in plated monolayer format
Species-species comparisons
Genomics

Content & Storage

Content

1 x Cryopreserved ampule of C57BL/6 Mouse Hepatocytes (plateable format) containing > 5 million viable cells

Instructions, SDS, CoA

Related Products

Mouse C57BL/6 Hepatocytes, Cryopreserved, Plateable

Human Hepatocytes, Cryopreserved, Plateable HUCPG

人肝细胞，冷冻保存，可电镀 HUCPG

Human Hepatocytes, Cryopreserved, Plateable and Interaction Qualified HUCPI

人肝细胞，冷冻保存，可电镀和相互作用合格的 HUCPI

lonza,lonza CC-4098,lonza CC-3121,原代细胞和干细胞,Bovine Brain Extract (BBE)

货号: CC-4098

Bovine Brain Extract contains endothelial cell proliferation growth factors, 9 mg/mL, 5 mL

牛脑提取物含有内皮细胞增殖生长因子，9 mg/mL，5 mL

Product Overview

Bovine Brain Extract is useful in the culture of various endothelial cell types. The product is offered at a concentration of 9 mg/mL.

牛脑提取物可用于培养各种内皮细胞类型。该产品的浓度为 9 mg/mL。

Content & Storage

Content

1 x Bottle containing 9 mg/mL Bovine Brain Extract, 5 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

EBM™ Endothelial Cell Growth Basal Medium, 500 mL CC-3121

EBM™ 内皮细胞生长基础培养基，500 mL CC-3121

Bovine Brain Extract (BBE), 2 ml CC-4092

牛脑提取物 (BBE)，2 ml CC-4092

lonza,lonza CC-3209,lonza PT-2599,NPMM™ 神经祖细胞维护培养基 BulletKit™

Catalog #: CC-3209

Culture system containing PBM^TM Neural Progenitor Basal Medium (CC-3210), NPMM^TMMaintenance Media SingleQuots^TM (CC-4241) and Neural Progenitor Supplement SingleQuots^TM (CC-4242) required for growth and differentiation of neural progenitor cells

包含神经祖细胞生长和分化所需的 PBMTM 神经祖细胞基础培养基 (CC-3210)、NPMMTM 维持培养基 SingleQuotsTM (CC-4241) 和神经祖细胞补充剂 SingleQuotsTM (CC-4242) 的培养系统

Product Overview

Neural Progenitor Maintenance Medium (NPMM) BulletKit^TM contains the necessary supplements and media for optimal normal human neural progenitor cell growth and differentiation.

神经祖细胞维持培养基 (NPMM) BulletKitTM 包含必要的补充剂和培养基，以实现最佳的正常人类神经祖细胞生长和分化。

Content & Storage

Content

1 x NPBM^TM Neural Progenitor Basal Medium (CC-3210), 200 mL

1 x NPMM^TM Neural Progenitor Maintenance Medium SingleQuots^TM Kit (CC-4241) containing:

1 x hEGF, 0.40 mL

1 x hFGF, 0.40 mL

1 x NPMM^TM Neural Progenitor Supplement SingleQuots^TMKit (CC-4242) containing:

1 x NSF-1, 4.0 mL

1 x GA-1000, 0.40 mL

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

NPMM™ Neural Progenitor Maintenance Medium BulletKit™

NHNP – Human Neural Progenitor Cells PT-2599

NHNP – 人类神经祖细胞 PT-2599

lonza,lonza CC-4539,lonza CC-3118,S-ALI™ 小气道气液接口 Medium BulletKit™

Catalog #: CC-4539

Culture system containing S-ALI^TM Basal Media (CC-3281), S-ALI^TM Differentiation Media (CC-3282) and S-ALI^TM SingleQuots^TM supplements (CC-4538) required for air-liquid interface differentiation of small airway epithelial cells.

包含小气道上皮细胞气液界面分化所需的 S-ALITM 基础培养基 (CC-3281)、S-ALITM 分化培养基 (CC-3282) 和 S-ALITM SingleQuotsTM 补充剂 (CC-4538) 的培养系统。

Product Overview

S-ALI^TM BulletKit^TM Small Airway Air-Liquid Interface BulletKit^TM provides scientists with a unique tool to investigate new areas of airway epithelial research in a more physiologically relevant way.

By using an air-liquid interface model, the next generation tool of in vitro respiratory research, scientists can explore small airway epithelial cell differentiation and apply therapies in novel ways. The S-ALI^TM Bullekit^TM is made up of a Growth and Differentiation Medium SingleQuots^TM Kit, Growth Basal Medium and Differentiation Basal Medium.

Our S-ALI^TM BulletKit^TM promotes full differentiation of the small airway epithelium expressed by the formation of a polarized epithelium with good barrier function (transepithelial resistance), secretory phenotype (mucin secretion) and ciliogenesis. When used with our guaranteed small airway epithelial cells (CC-2547S), differentiation will occur by day 22 in culture.

S-ALITM BulletKitTM Small Airway Air-Liquid Interface BulletKitTM 为科学家提供了一种独特的工具，可以以更生理相关的方式研究气道上皮研究的新领域。

通过使用气液界面模型（体外呼吸研究的下一代工具），科学家们可以探索小气道上皮细胞分化并以新的方式应用治疗。 S-ALITM BullekitTM 由生长和分化培养基 SingleQuotsTM 试剂盒、生长基础培养基和分化基础培养基组成。

我们的 S-ALITM BulletKitTM 促进小气道上皮的完全分化，通过形成具有良好屏障功能（跨上皮阻力）、分泌表型（粘蛋白分泌）和纤毛发生的极化上皮表达。当与我们保证的小气道上皮细胞 (CC-2547S) 一起使用时，将在培养的第 22 天发生分化。

Content & Storage

Content

1 x S-ALI^TM Growth Basal Media (CC-3281), 250 mL

1 x S-ALI^TM Differentiation Media (CC-3282), 500 mL

1 x S-ALI^TM SingleQuots^TM Supplement Pack (CC-4538) containing:

1 x Natural Cap Vial with Transferrin, 0.75 mL

1 x Amber Vial with S-ALI-Inducer, 1.00 mL

1 x Orange Cap Vial with BPE, 3.00 mL

1 x Amber Vial with Epinephrine, 0.75 mL

1 x Red Cap Vial with GA-1000, 0.75 mL

1 x Green Cap Vial hEGF, 0.75 mL

1 x Natural Cap Vial with Hydrocortisone, 0.75 mL

1 x Lilac Cap Vial with Insulin, 0.75 mL

1 x Amber Vial with Retinoic Acid, 0.75 mL

1 x Bottle Fatty Acid Free BSA-FAF, 7.50 mL

1 x Amber Vial with Triiodothyronine, 0.75 mL

Related Products

S-ALI™ Small Airway Air-Liquid Interface Medium BulletKit™

SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit™ CC-3118

SAGM™ 小气道上皮细胞生长培养基 BulletKit™ CC-3118

lonza,lonza CC-2902,原代细胞和干细胞,InMyoFib-人类肠道肌成纤维细胞

货号: CC-2902

Cryopreserved ampule of Human Intestinal Myofibroblasts (InMyoFib) containing ≥500,000 cells

Help us improve our primary cell offering. Invest 5 minutes to share your specific needs with us. Fill out our survey and enter the chance to win an amazon voucher.

含有≥500,000 个细胞的人肠道肌成纤维细胞 (InMyoFib) 的冷冻保存安瓿

帮助我们改进我们的主要细胞产品。花 5 分钟时间与我们分享您的特定需求。填写我们的调查表并有机会赢取亚马逊代金券。

Product Overview

Lonza offers Human Intestinal Myofibroblasts (H-InMyoFib), cryopreserved at passage 2 with ≥500,000 cells per vial. H-InMyoFibs share phenotypic characteristics of both fibroblasts and smooth muscle cells but do not express smooth muscle markers such as smoothelin, caldesmon, or (in the normal colon and small intestine) desmin. They play a fundamental role in growth and development of intestine, immunology, inflammation, fibrosis, water and electrolyte transport, contraction and wound healing.

Cryopreserved Clonetics^TM Human Intestinal Myofibroblasts (H-InMyoFib) are guaranteed through 15 population doublings, express alpha SM Actin after thaw ≥70% and Desmin ≤10%. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Recommended Medium: SMGM^TM-2 BulletKit^TM

Lonza 提供人类肠道肌成纤维细胞 (H-InMyoFib)，在第 2 代冷冻保存，每瓶 ≥500,000 个细胞。 H-InMyoFibs 具有成纤维细胞和平滑肌细胞的表型特征，但不表达平滑肌标志物，如平滑素、caldesmon 或（在正常结肠和小肠中）结蛋白。它们在肠道的生长和发育、免疫学、炎症、纤维化、水和电解质运输、收缩和伤口愈合中发挥着重要作用。

冷冻保存的 CloneticsTM 人肠道肌成纤维细胞 (H-InMyoFib) 保证通过 15 次群体倍增，解冻后表达 α SM 肌动蛋白≥70% 和 Desmin ≤10%。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

推荐培养基：SMGMTM-2 BulletKitTM

Benefits

Absorption studies & toxicology pertaining to oral medications
Inflammation
Fibrosis
Water & electrolyte transport
Contraction & peristalsi
Wound healing
Neoplasia & intestinal polyp formation
Crohn’s disease
Toxicology and cytotoxicity
Gene expression

Content & Storage

Content

1 x Cryopreserved ampule InMyoFib containing ≥500,000 cells

Instructions, SDS, CoA

Related Products

InMyoFib – Human Intestinal Myofibroblasts

RPTEC – Human Renal Proximal Tubule Epithelial Cells CC-2553

RPTEC – 人肾近端小管上皮细胞 CC-2553

InEpC – Human Intestinal Epithelial Cells CC-2931

InEpC – 人肠上皮细胞 CC-2931

Human Intestinal Epithelial and Myofibroblast Cell Combo CC-4540

人肠上皮和肌成纤维细胞组合 CC-4540

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

lonza,lonza 2W-400C,lonza CC-2702,原代细胞和干细胞,人外周血 CD14+ 单核细胞

Catalog #: 2W-400C

Cryopreserved ampule of Human Peripheral Blood CD14+ Monocytes, positive selection, containing ≥10 million cells

人外周血 CD14+ 单核细胞冻存安瓿，阳性选择，含有≥1000 万个细胞

Product Overview

Cells You Can Count on to Perform

Choices in Cell Type and Tissue Source

Supported by Lonza Media

The recommended medium varies by application (see below). Please contact Scientific Support for additional culturing recommendations. Please note, media listed for macrophage and dendritic cell differentiation are recommendations only.

Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
Dendritic Cells: LGM-3^TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis (CoA) is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of CD14+ Monocytes containing ≥ 10 million cells

Instructions, SDS, CoA

Related Products

Human Peripheral Blood CD14+ Monocytes, Cryopreserved, Positive Selection, 10 Million

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 50 million CC-2702

人外周血单个核细胞 (hPBMC)，冷冻保存，5000 万个 CC-2702

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

Data

CC-3208,lonza CC-3207,原代细胞和干细胞,OBM™ 成骨细胞生长和分化基础培养基

Catalog #: CC-3208

OBM^TM Osteoblasts Basal Medium

Product Overview

OBMTM 是一种成骨细胞生长基础培养基，单独出售。这可以与 1 套 CC-4193（成骨细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。由基础培养基和补充剂组成的完整 OGMTM BulletKitTM 可以使用目录号 CC-3207 一起购买。

CloneticsTM OGMTM 成骨细胞生长培养基专为人类成骨细胞的生长和扩增而配制。该试剂盒含有 10% 的血清。 OGMTM 分化补充剂也包括在内，建议用于分化测定。该试剂盒包装方便，包括含有生长和分化补充剂的基础培养基，用于成骨细胞的完全培养

Benefits

Applications:

In vitro bone models
Mineralization
Osteoporosis
Gene expression
Hormone treatments
Osteopenia
Paget’s Disease

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products

OBM™ Osteoblast Growth and Differentiation Basal Medium

NHOst – Human Osteoblasts CC-2538

NHOst – 人类成骨细胞 CC-2538

OGM™ Osteoblast Growth Medium BulletKit™ CC-3207

OGM™ 成骨细胞生长培养基 BulletKit™ CC-3207

lonza PT-4124,原代细胞和干细胞,用于 hMSC 软骨分化试剂盒的 TGF Beta 3

Catalog #: PT-4124

软骨分化所需的 TGF Beta-3，2 µg。用于 hMSC 软骨分化 BulletKitTM，PT-3003（单独出售）

Product Overview

TGF Beta 3 required for use with the hMSC Chondrogenic Differentiation BulletKit^TM (PT-3003).

Content & Storage

Content

1 x Lyophilized TGF Beta 3, 2 µg

Related Products

TGF Beta 3 for hMSC Chondrogenic Differentiation Kit

NHAC-kn – Human Knee Articular Chondrocytes CC-2550

NHAC-kn – 人膝关节软骨细胞 CC-2550

CGM™ Chondrocyte Growth Medium BulletKit™ CC-3216

CGM™ 软骨细胞生长培养基 BulletKit™ CC-3216

CDM™ Chondrocyte Differentiation Medium BulletKit™ CC-3225

CDM™ 软骨细胞分化培养基 BulletKit™ CC-3225

Chondrocyte ReagentPack™ Subculture Reagents CC-3233

软骨细胞 ReagentPack™ 继代培养试剂 CC-3233

DPSC – Human Dental Pulp Stem Cells PT-5025

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza CC-3101,lonza原代细胞和干细胞,KBM™ 角质形成细胞基础培养基

Catalog #: CC-3101

推荐用于人角质形成细胞生长的 KBMTM 角质形成细胞基础培养基（仅基础培养基），500 mL。所需的生长因子和补充剂单独出售 (CC-4131)。

Product Overview

KBMTM 是一种单独出售的基础培养基，用于在无血清环境中增殖角质形成细胞。这可以与 CC-4131（角质形成细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。或者，这两种成分可以作为 KGMTM 角质形成细胞生长培养基 BulletKitTM 一起购买。

Content & Storage

Content

1 x Bottle KBM^TM Basal Medium, 500 mL

lonza M-FB-481,原代细胞和干细胞,小鼠胚胎成纤维细胞 (MEF)

Catalog #: M-FB-481

Cryopreserved ampule of Mouse Embryonic Fibroblasts containing ≥2 million cells

Product Overview

冷冻保存的小鼠原代胚胎成纤维细胞 (MEF) 从 CD-1 小鼠胚胎中分离出来，通过培养扩增，然后冷冻保存。

细胞可保证 5 次群体倍增，并显示出与新鲜细胞相当的形态和生长特性。 CloneticsTM MEF 方便易用，使研究人员能够专注于结果。

推荐培养基：Lonza’s DMEM High Glucose w/ L-Gln，500 mL（第 12-604F 部分），含 10% FBS（Sigma-Aldrich 第 F6178 部分）

Benefits

Ready-To-Use
No tissue dissection or animal handling required
Increased data reproducibility
Characteristic morphology and staining tests confirm tissue origin and cell type
Negative for mycoplasma, bacteria, yeast and fungi
Certificate of Analysis (CoA) is available for every batch

Content & Storage

Content

≥ 2 million cryopreserved cells in a 1.0 mL cell suspension

Related Products

P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4012

P4 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-4012

P4 Primary Cell 4D-Nucleofector™ X Kit L V4XP-4024

P4 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-4024

P4 Primary Cell 4D-Nucleofector™ X Kit S V4XP-4032

P4 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-4032

P4 Primary Cell 96-well Nucleofector™ Kit V4SP-4096

P4 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-4960

P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4002

P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4002

P4 Primary Cell 384-well Nucleofector™ Kit V5SP-4010

P4 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-4010

lonza CC-4009,原代细胞和干细胞,牛垂体提取物 (BPE)

Catalog #: CC-4009

Bovine Pituitary Extract (BPE), 13mg/mL, 2 mL

Product Overview

牛垂体提取物可用于培养各种上皮细胞类型。该产品包含在与用于上皮细胞类型的各种培养基系统相关的 SingleQuotsTM 试剂盒中。该产品的浓度为 13 mg/ml。

Content & Storage

Content

1 x Vial Containing 13 mg/mL BPE 2 mL

lonza CC-3246,原代细胞和干细胞,SKBM™- 2 骨骼肌细胞生长基础培养基-2

Catalog #: CC-3246

SkBM^TM-2 Skeletal Muscle Myoblasts Basal Medium for culture of human skleletal muscle mycoblasts, 500 mL

Product Overview

SkBMTM -2 是一种骨骼肌成肌细胞基础培养基，单独出售。这可以与 1 套 CC-3244（骨骼肌成肌细胞生长培养基 SingleQuotsTM 补充剂和生长因子）一起使用。由基础培养基和补充剂组成的完整 SkGMTM -2 BulletKitTM 可以使用目录号 CC-3245 一起购买。我们提供两种骨骼肌生长培养基 – SkGMTM 和 SkGMTM-2。

SkGMTM-2 骨骼肌细胞生长培养基-2（目录号 CC-3245）是 SkGMTM 培养基的增强版。胎球蛋白已被去除，基础培养基也得到了进一步优化。

SkGMTM 培养基经过优化，可用于人体骨骼肌细胞。 SkGMTM -2 专为与正常和糖尿病骨骼肌成肌细胞一起使用而设计

Content & Storage

Content

1 x Bottle Basal Medium 500 mL

Related Products

SKBM™- 2 Skeletal Muscle Cell Growth Basal Medium-2

HSMM – Human Skeletal Muscle Myoblasts CC-2580

HSMM – 人体骨骼肌成肌细胞 CC-2580

D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type I CC-2900

D-HSMM – 患病的人体骨骼肌成肌细胞 – I 型糖尿病 CC-2900

D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type II CC-2901

D-HSMM – 患病的人体骨骼肌成肌细胞 – II 型糖尿病 CC-2901

SkGM™-2 Skeletal Muscle Cell Growth Medium-2 BulletKit™ CC-3245

SkGM™-2 骨骼肌细胞生长培养基-2 BulletKit™ CC-3245

lonza,lonza CC-2551B,原代细胞和干细胞,乳腺上皮细胞培养试剂盒

货号: CC-2551B

Mammary Epithelial Cell Culture Kit

乳腺上皮细胞培养试剂盒

Product Overview

Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGM^TM^TM BulletKit^TM Mammary Epithelial Cell Growth Media (catalog no. CC-3150)

购买我们的乳腺上皮细胞培养试剂盒（目录号 CC-2551B）并获得细胞和培养基的折扣价。该试剂盒由冷冻保存的乳腺上皮细胞安瓿（货号 CC-2551）和 MEGMTMTM BulletKitTM 乳腺上皮细胞生长培养基（货号 CC-3150）组成

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial
1 x Bottle Basal Medium 500 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – German

SDS – Italian

SDS – Dutch

SDS – French

SDS – Spanish

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza CC-3205,lonza CC-4181,CC-3204,SCGM™ 基质细胞生长培养基 BulletKit™

货号: CC-3205

SCGM^TM Stromal Cell BulletKit^TM

SCGMTM 基质细胞 BulletKitTM

Product Overview

Stromal Cell Growth Medium was developed to support the maintenance and expansion of prostate stromal cells and certain aortic and dental fibroblasts. SCGM^TMmedium contains 5% FBS.

Clonetics^TM Media Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems.

基质细胞生长培养基用于支持前列腺基质细胞和某些主动脉和牙成纤维细胞的维持和扩增。 SCGMTMmedium 含有 5% FBS。

CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。媒体系统已针对各种细胞系统专门开发和优化。

Content & Storage

Content

1 x Bottle Basal Medium 500 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
1 x Gray Cap Vial with hFGF-B 0.50 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Bottle FBS 25.00 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – German

SDS – Italian

SDS – Dutch

SDS – French

SDS – Spanish

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

SCGM™ Stromal Cell Growth Medium BulletKit™

AoAF – Human Aortic Adventitial Fibroblasts CC-7014

AoAF – 人主动脉外膜成纤维细胞 CC-7014

PrSC – Human Prostate Stromal Cells CC-2508

PrSC – 人类前列腺基质细胞 CC-2508

HPdLF – Human Periodontal Ligament Fibroblasts CC-7049

HPdLF – 人牙周膜成纤维细胞 CC-7049

SCBM™ Stromal Cell Growth Basal Medium CC-3204

SCBM™ 基质细胞生长基础培养基 CC-3204

SCGM™ Stromal Cell Growth Medium SingleQuots™ Supplements and Growth Factors CC-4181

SCGM™ 基质细胞生长培养基 SingleQuots™ 添加剂和生长因子 CC-4181

lonza,lonza 2M-125E,原代细胞和干细胞,人骨髓单个核细胞 (MNC)

货号: 2M-125E

Cryopreserved human bone marrow mononuclear cells, 300 million cells

冷冻保存的人骨髓单个核细胞，3亿个细胞

Product Overview

Bone marrow mononuclear cells are a mixed population of single nucleus cells including monocytes, lymphocytes, and hematopoietic stem and progenitor cells.

骨髓单核细胞是单核细胞的混合群体，包括单核细胞、淋巴细胞以及造血干细胞和祖细胞。

BMNCs You Can Count on to Perform

Fresh BMNC orders require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.

Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. Minimum order quantity of 2 vials per donor is required for fresh cells. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.’

通过密度梯度分离从全骨髓中分离冷冻保存的龙沙骨髓单核细胞。分离单核细胞消除红细胞和粒细胞，产生更稳定的细胞产物。 Lonza BMNCs 可从大量多样的供体库中获得。根据购买的产品，它们保证每个安瓿含有 ≥5、25、100 或 2 亿个活细胞。

新鲜的 BMNC 订单要求每个捐赠者至少订购两瓶。有关新鲜细胞的尺寸和价格信息，请联系客户服务。

只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。如果您在选择合适的培养基、试剂或方案方面需要帮助，请联系 Lonza Scientific Support。对于新鲜细胞，每个供体最少需要订购 2 瓶。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。

Content & Storage

Content

1 cryopreserved ampule containing ≥ 300 million cells

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – Dutch

SDS – German

SDS – Italian

SDS – Spanish

SDS – French

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

lonza CC-3244,SKGM™ – 2 种骨骼肌细胞生长培养基-2 SingleQuots™ 补充剂和生长因子

货号: CC-3244

SkGM^TM-2 Skeletal Muscle Myoblasts SingleQuots^TM Kits, Supplements and growth factors required for culture of Human Skeletal Muscle Myoblasts (SkBM-2^TM Basla Medium (CC-3246) sold separately)

人骨骼肌成肌细胞培养所需的 SkGMTM-2 骨骼肌成肌细胞 SingleQuotsTM 试剂盒、补充剂和生长因子（SkBM-2TM Basla 培养基 (CC-3246) 单独出售）

Product Overview

SkGM^TM -2 SingleQuot^TM Kit are components sold separately and does not include the basal medium. These are part of the SkGM^TM -2 Skeletal Muscle Myoblast Medium BulletKit^TM (Catalog Number CC-3245). The full SkGM^TM -2 BulletKit^TM consisting of basal medium and supplements can be purchased together using the catalog number CC-3245. We offers two varieties of Skeletal Muscle Growth Medium – SkGM^TM and SkGM^TM-2.

SkGM^TM-2 Skeletal Muscle Cell Growth Medium-2 is an enhancement of SkGM^TM Medium. Fetuin has been removed and there has been further optimization of the basal media.

SkGM^TM Medium is optimized for use with human skeletal muscle cells. SkGM^TM -2 is specially designed for use with normal and diabetic skeletal muscle myoblasts.

SkGMTM -2 SingleQuotTM 套件是单独出售的组件，不包括基础培养基。这些是 SkGMTM -2 骨骼肌成肌细胞培养基 BulletKitTM（目录号 CC-3245）的一部分。由基础培养基和补充剂组成的完整 SkGMTM -2 BulletKitTM 可以使用目录号 CC-3245 一起购买。我们提供两种骨骼肌生长培养基 – SkGMTM 和 SkGMTM-2。

SkGMTM-2 骨骼肌细胞生长培养基-2 是 SkGMTM 培养基的增强型。胎球蛋白已被去除，基础培养基也得到了进一步优化。

SkGMTM 培养基经过优化，可用于人体骨骼肌细胞。 SkGMTM -2 专为与正常和糖尿病骨骼肌成肌细胞一起使用而设计。

Content & Storage

Content

1 x Red Cap Vial with GA-1000 0.50 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Natural Cap Vial with Dexamethasone 0.50 mL
1 x Bottle with FBS 50.00 mL
1 x Bottle L-Glutamine 10.00 mL

Instructions, SDS, CoA

Safety Data Sheets (SDS)

View SDS

SDS – German

SDS – Italian

SDS – Dutch

SDS – French

SDS – Spanish

SDS – English

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Related Products

SKGM™ - 2 Skeletal Muscle Cell Growth Medium-2 SingleQuots™ Supplements and Growth Factors

HSMM – Human Skeletal Muscle Myoblasts CC-2580

HSMM – 人体骨骼肌成肌细胞 CC-2580

D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type I CC-2900

D-HSMM – 患病的人体骨骼肌成肌细胞 – I 型糖尿病 CC-2900

D-HSMM – Diseased Human Skeletal Muscle Myoblasts – Diabetes Type II CC-2901

D-HSMM – 患病的人体骨骼肌成肌细胞 – II 型糖尿病 CC-2901

SkGM™-2 Skeletal Muscle Cell Growth Medium-2 BulletKit™ CC-3245

SkGM™-2 骨骼肌细胞生长培养基-2 BulletKit™ CC-3245

lonza CC-2547S,lonza CC-2540,CC-2547,S-ALITM 小气道气液界面培养基的小气道上皮细胞

货号: CC-2547S

SAEC, Small Airway Epithelial Cells, for ALI Culture

Overview

Product Overview

Lonza’s Human Small Airway Epithelial Cells (SAEC) are isolated from normal human lung tissue the distal portion of the lung in the 1mm bronchiole area. Contact our Scientific Support team for additional details.

Cryopreserved Clonetics^TM SAECs are guaranteed through 15 population doublings and stain positive for cytokeratin 19. SAECs labeled CC-2547S are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation, mucin production, andTEER levels. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B andhepatitis C are not detected for all donors and/or cell lots. A Certificate ofAnalysis is provided for each cell lot purchased.

Lonza 的人小气道上皮细胞 (SAEC) 是从正常人肺组织中分离出来的，位于 1mm 细支气管区域的肺远端部分。有关更多详细信息，请联系我们的科学支持团队。

冷冻保存的 CloneticsTM SAEC 保证通过 15 次群体倍增并且对细胞角蛋白 19 染色呈阳性。标记为 CC-2547S 的 SAEC 适用于气液界面研究，并保证以纤毛形成、粘蛋白产生和 TEER 水平为标志的分化。所有细胞的支原体、细菌、酵母和真菌均呈阴性。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为每个购买的细胞批次提供一份分析证书。

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Instructions, SDS, CoA

Related Products

SAEC – Small Airway Epithelial Cells for S-ALITM Small Airway Air-Liquid-Interface Medium

SAEC – Human Small Airway Epithelial Cells CC-2547

SAEC – 人类小气道上皮细胞 CC-2547

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid CC-2540

NHBE – 含有维甲酸 CC-2540 的人支气管/气管上皮细胞

DHLF-IPF – Diseased Human Lung Fibroblasts, Idiopathic Pulmonary Fibrosis CC-7231

DHLF-IPF – 患病的人肺成纤维细胞，特发性肺纤维化 CC-7231

lonza 4W-270,lonza 人外周血单个核细胞 (hPBMC)

货号: 4W-270

Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 10 million cells

含有 ≥ 1000 万个细胞的人外周血单个核细胞 (hPBMC) 冻存安瓿

Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC pools contain, on average, 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. Cells are cryopreserved in Lonza cryopreservation media. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.

Manufactured by AllCells^®

来自白细胞分离术的富含单核细胞 (MNC) 的细胞耗尽了红细胞和血小板。 MNC 池平均含有 50% 的 T 细胞、20% 的单核细胞、10% 的 B 细胞、10% 的 NK 细胞，并且可能含有高达 5% 的粒细胞。细胞在 Lonza 冷冻保存培养基中冷冻保存。使用 AO/PI 确定计数和活力。按照 IRB 协议从健康供体收集细胞。

由 AllCells® 制造

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis (CoA) is provided for each cell lot purchased
支原体、细菌、酵母菌和真菌检测呈阴性
并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎
为购买的每个细胞批次提供分析证书 (CoA)

Applications

ELISPOT
Proliferation assays
Immunogenicity assays
Cell therapy process development
Humanized mice
Biomarker discovery

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 10 million cells

Instructions, SDS, CoA

lonza 4W-270C,lonza 人外周血单个核细胞 (hPBMC)

货号: 4W-270C

Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells

含有 ≥ 1 亿个细胞的人外周血单个核细胞 (hPBMC) 冻存安瓿

Product Overview

Manufactured by AllCells^®

由 AllCells® 制造

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

ELISPOT
Proliferation assays
Immunogenicity assays
Cell therapy process development
Humanized mice
Biomarker discovery

Content & Storage

Content

1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells

Instructions, SDS, CoA

lonza 4W-820,lonza 原代细胞和干细胞,lonza 外周血人血清

Catalog #: 4W-820

Human serum isolated from peripheral blood, frozen

Product Overview

来自健康供体的全血清从血清分离采血管中分离出来。分装成 3mL 标称体积（取决于供体）并在 -80ºC 下速冻。按照批准的 IRB 协议收集血液。由 AllCells® 制造

Content & Storage

Content

– singe tube of frozen human serum, 3mL nominal volume

lonza 2M-101C,lonza 原代细胞和干细胞,人骨髓 CD34+ 祖细胞

Catalog #: 2M-101C

冷冻保存的人骨髓 CD34+ 祖细胞安瓿，含有 ≥ 100 万个细胞。使用阳性免疫磁性选择分离单核细胞。

Product Overview

骨髓 CD34+ 细胞含有造血干细胞和祖细胞，并且已知可分化成所有不同的血细胞类型。

您可以信赖的纯度和活力
Lonza骨髓CD34+细胞通过阳性免疫磁分离从骨髓单个核细胞（MNC）中分离出来，保证纯度≥90%。根据订购的产品，它们保证每个安瓿含有≥100,000、≥300,000、≥500,000、≥100 万或 ≥200 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、丙型肝炎和乙型肝炎。为购买的每个细胞批次提供分析证书。提供各种大小的新鲜骨髓 CD34+ 细胞，每个供体至少需要两瓶。有关新鲜细胞的尺寸和价格信息，请联系客户服务。

由优化的培养系统支持
建议使用 HPGMTM 造血生长培养基以在无血清培养中获得最佳性能（根据应用可能需要额外的细胞因子）也可以接受含有血清的替代培养基。有关含血清培养基的建议，请联系科学支持。

细胞类型和组织来源的选择
复杂的研究通常需要灵活性。这就是为什么我们提供分离和纯化的骨髓 CD34+ 细胞以及未加工的骨髓，以防您需要分离自己的细胞。骨髓 CD34+ 细胞非常适用于需要来自单个供体的大量细胞的研究或调查不同年龄、性别或种族供体的细胞差异的研究。但是，如果您的研究需要更多幼稚细胞，脐带血 CD34+ 细胞可能是更好的选择。

Benefits

Ready to use
High purity with > 90% CD34+ cells
Wide donor variety
Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34⁺ cells)

Content & Storage

Content

1 x Cryopreserved ampule of Human Bone Marrow CD34+ containing ≥1 million cells

Related Products

Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 1 Million

Human Bone Marrow Stromal Cells, 5 Million 2M-302

人骨髓基质细胞，500 万个 2M-302

Unprocessed Human Bone Marrow, Fresh, 10 mL 1M-105

未加工的人骨髓，新鲜，10 mL 1M-105

hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

MycoAlert^® Mycoplasma Detection Kit (25 Tests) LT07-218

MycoAlert® 支原体检测试剂盒（25 次测试）LT07-218

lonza 00192627B,lonza原代细胞和干细胞,成人角质形成细胞培养试剂盒

Catalog #: 00192627B

Adult Keratinocyte Cell Culture Kit

Product Overview

购买我们的成人角质形成细胞培养试剂盒（目录号 192627B）并获得细胞和培养基的折扣价。该试剂盒由冷冻保存的成人角质形成细胞安瓿（目录号 192627）和 KGMTM-Gold BulletKitTM 角质形成细胞生长培养基（目录号 192060）组成

Content & Storage

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial
1 x Bottle Basal Medium 500 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.25 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL

lonza PT-3005,lonza 原代细胞和干细胞,lonza 人牙髓干细胞 BulletKit™

Catalog #: PT-3005

Human Dental Pulp Stem Cell BulletKit^TM

Product Overview

DPSC 牙髓干细胞 BulletKitTM 培养基； DPSC 牙髓干细胞 BulletKitTM 培养基以 BulletKitTM 培养基（目录号 PT-3005）的形式提供，其中包括基础培养基和人牙髓衍生间充质干细胞增殖所需的补充剂。基础培养基（DPSC 基础培养基；目录号 PT-3927）和必要的补充剂（DPSC SingleQuot 试剂盒；目录号 PT-4516）也可以单独购买。

Related Products

DPSC – Human Dental Pulp Stem Cells PT-5025

DPSC – 人牙髓干细胞 PT-5025

lonza PT-3102B,lonza 原代细胞和干细胞,lonza 成脂诱导培养基

Catalog #: PT-3102B

Induction Medium for adipogenic differentiation of mesenchymal stem cells

Product Overview

成脂诱导培养基是 hMSC 成脂分化 BulletKitTM 的一个组成部分，可在此处单独购买。

干细胞具有分化成包括脂肪细胞在内的多种细胞谱系的独特能力。 Lonza 的 hMSC 成脂分化 BulletKitTM 培养基是一种含血清的培养基系统，旨在诱导人骨髓来源的间充质干细胞在短短 19 天内成脂分化成成熟的、功能活跃的脂肪细胞。然后可以通过多种方法验证分化，包括使用 Lonza 的 AdipoRedTM 脂肪生成分析试剂，该试剂提供与脂肪生成分化量相关的定量值。

用 hMSC 脂肪分化 BulletKitTM 培养基分化的部分细胞列表*：

人骨髓来源的干细胞
C57BL/6 小鼠骨髓干细胞
人类脂肪干细胞
人牙周膜来源干细胞
人腘绳肌腱干细胞
hMSC 成脂分化 BulletKitTM 培养基提供诱导培养基和维持培养基，保证诱导人骨髓间充质干细胞成脂分化成成熟的、功能活跃的脂肪细胞；每瓶 hMSC 脂肪分化 BulletKitTM 培养基都含有足够的诱导培养基和维持培养基，可将大约 300 万个人骨髓来源的间充质干细胞 [6 孔板中大约 15 个孔] 完全分化为成熟的功能活跃的脂肪细胞。 hMSC 脂肪分化培养基以 BulletKitTM 培养基（目录号 PT-3004）的形式提供，其中包括用于诱导和维持的基础培养基以及用于脂肪形成诱导和维持人骨髓间充质干细胞的必要补充剂。

lonza CC-4068,原代细胞和干细胞,hFGF-人成纤维细胞生长因子CC-4068

Catalog #: CC-4068

hFGF, Human Fibroblast Growth Factors, 1 mL

Product Overview

成纤维细胞生长因子 (FGFs) 是参与血管生成、伤口愈合、胚胎发育和各种内分泌信号通路的生长因子的组合。 FGF 是肝素结合蛋白，与细胞表面相关的硫酸乙酰肝素蛋白聚糖的相互作用已被证明对 FGF 信号转导至关重要。 FGF 是多种细胞和组织增殖和分化过程中的关键参与者。重组纤维母细胞生长因子的浓度为 1µg/ml

Content & Storage

Content

1 x Vial Containing 1 ug/mL hFGF^TM, 1 mL

lonza PT-5001,原代细胞和干细胞,HprAD-人皮下前脂肪细胞

Catalog #: PT-5001

Cryopreserved ampule of Human Subcutaneous Preadipocytes containing ≥4 million cells

Product Overview

PoieticsTM 正常人前脂肪细胞 (HprAD) 是从正常（非糖尿病）供体的皮下或内脏脂肪中分离出来的。来自糖尿病供体的前脂肪细胞可作为单独的产品获得。

相关的额外捐赠者信息，包括体重指数 (BMI) 可通过联系科学支持获得。

Recommended Media and Reagents

PGM^TM -2 Preadipocyte Growth Medium-2 BulletKit^TM Medium (provides both a proliferation medium and a differentiation medium guaranteed to induce differentiation into mature, functionally active adipocytes; Each bottle of the PGM-2^TM BulletKit^TM Medium contains enough differentiation medium to fully differentiate 10 million preadipocytes [10 complete 96-well plates] into mature, functionally active adipocytes).

Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
A Certificate ofAnalysis is provided for each cell lot purchased

Content & Storage

Content

1 x Cryopreserved ampule of Subcutaneous Preadipocytes containing ≥ 4 million cells

Related Products

HprAD - Human Subcutaneous Preadipocyte Cells

PBM-2 Preadipocyte Basal Medium-2 PT-8202

PBM-2 前脂肪细胞基础培养基-2 PT-8202

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1012

P1 原代细胞 4D-Nucleofector™ X 试剂盒 L V4XP-1012

P1 Primary Cell 4D-Nucleofector™ X Kit L V4XP-1024

P1 Primary Cell 4D-Nucleofector™ X Kit S V4XP-1032

P1 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-1032

P1 Primary Cell 96-well Nucleofector™ Kit V4SP-1096

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 V4SP-1096

P1 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-1960

P1 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-1960

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1002

P1 Primary Cell 384-well Nucleofector™ Kit V5SP-1010

P1 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-1010

Human Visceral Preadipocyte Cells PT-5005

人内脏前脂肪细胞 PT-5005

Human Subcutaneous Preadipocyte Cells PT-5020

人皮下前脂肪细胞 PT-5020

AdipoRed™ Assay Reagent, 20 mL PT-7009

hADSC Human Adipose-Derived Stem Cells PT-5006

hADSC 人体脂肪干细胞 PT-5006

hMSC – Human Mesenchymal Stem Cells PT-2501

hMSC – 人间充质干细胞 PT-2501

Diseased Human Visceral Preadipocyte Cells – Diabetes Type I PT-5023

患病的人类内脏前脂肪细胞 – I 型糖尿病 PT-5023

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type I PT-5021

患病的人类皮下前脂肪细胞 – I 型糖尿病 PT-5021

Diseased Human Subcutaneous Preadipocyte Cells – Diabetes Type II PT-5022

患病的人类皮下前脂肪细胞 – II 型糖尿病 PT-5022

lonza CC-2903,NHCF-A人心房心脏成纤维细胞,NHCF-V人心室心脏成纤维细胞 CC-2904

Catalog #: CC-2903

Cryopreserved ampule of Normal Human Atrial Cardiac Fibroblasts (NHCF-A) containing ≥ 500,000 cells

Product Overview

Applications

Vascular diseases
Neointimal formation
Vascular remodeling
Atherosclerosis
Hypertension
Myocardial repair
Extracellular maintenance during cardiac cell / tissue growth
Cardiac Fibrosis Paracrine signalling
Toxicology
Gene expression

Content & Storage

Content

1 x Cryopreserved ampule of NHCF-A containing ≥ 500,000 cells

Related Products

NHCF-A – Human Atrial Cardiac Fibroblasts

NHCF-V – Human Ventricular Cardiac Fibroblasts CC-2904

NHCF-V – 人心室心脏成纤维细胞 CC-2904

AoAF – Human Aortic Adventitial Fibroblasts CC-7014

HAEC – Human Aortic Endothelial Cells CC-2535

HAEC – 人主动脉内皮细胞 CC-2535

AoSMC – Human Aortic Smooth Muscle Cells CC-2571

AoSMC – 人主动脉平滑肌细胞 CC-2571

FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™ CC-4526

FGM™-3 心脏成纤维细胞生长培养基-3 BulletKit™ CC-4526

lonza CC-4152,KGMTM-2 角质形成细胞生长培养基-2 SingleQuotsTM 补充剂和生长因子

Catalog #: CC-4152

KGM^TM-2 SingleQuots^TM Supplements Kit (KGM^TM-2 Gold Basal Medium (CC-3103) sold separately).

Product Overview

KGM-2 BulletkitTM，也作为单独的基础培养基和补充剂出售

CloneticsTM KGMTM-2 角质形成细胞生长培养基-2 已针对在无血清环境中的角质形成细胞增殖进行了优化，通常提供比 KGMTM 培养基更高的生长速率。

CloneticsTM 培养基产品专为支持人和动物原代细胞的生长而设计。媒体系统已针对各种细胞系统专门开发和优化。媒体以多种格式提供：

基础培养基 – 不含生长因子细胞因子或补充剂
SingleQuotsTM 试剂盒——生长因子、细胞因子和补充剂
BulletKitTM – 基础培养基和 SingleQuotsTM 套件包装在一起

lonza R-EDRG-515,原代细胞和干细胞,大鼠胚胎背根神经节(eDRG)神经元

Catalog #: R-EDRG-515

Cryopreserved Rat Embryonic Dorsal Root Ganglion ampule containing ≥ 1,000,000 cells

Product Overview

即用型大鼠胚胎背根神经节 (DRG) 神经元是通过标准化方法制备的高质量感觉神经元的悬浮液，可立即进行培养。

每瓶胚胎背根神经节神经元在 0.25 ml 悬浮液中含有大约 100 万个细胞。在电镀后的最初几天会发生一些细胞死亡，并且会观察到碎片。这很正常。培养大约 4 天后，细胞将形成神经突网络，到第 7 天，碎片将最少。在没有有丝分裂抑制剂的情况下，神经元倾向于聚集（神经节化）并从基质上分离。

大鼠胚胎背根神经节神经元 Tuj 染色阳性。原代神经元细胞需要合适的基质来粘附和存活。优选的底物是具有层粘连蛋白的聚-D-赖氨酸。聚-D-赖氨酸也可单独用于包被细胞培养塑料器皿或盖玻片。包被的细胞培养板、培养皿或盖玻片可以从供应商处购买，也可以在使用前立即制备。

推荐培养基：PNGMTM BulletKitTM 添加 100 ng/ml 大鼠神经生长因子-β (Sigma No. N2513)*

*重要提示：为了胚胎 DRG 神经元的存活和生长，始终需要向培养基中添加 100 ng/ml 的神经生长因子（Sigma No. N2513）。为了抑制雪旺氏细胞增殖，还需要添加有丝分裂抑制剂。

Benefits

Ready-To-Use
No tissue dissection or animal handling required
Increased data reproducibility
Characteristic morphology and staining tests confirm tissue origin and cell type
Negative for mycoplasma, bacteria, yeast and fungi
Certificate of Analysis (CoA) is available for every batch

Applications

Neurogenesis
Neurotransmitter Function
Gene Expression
Signaling Pathways
Electrophysiology
Neurotoxicity
Drug/Compound Screening
Advanced Cell Culture Models

Content & Storage

Content

≥ 1,000,000 cryopreserved cells per ampule

Related Products

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons

PNGM™ Primary Neuron Growth Medium BulletKit™ CC-4461

PNGM™ 原代神经元生长培养基 BulletKit™ CC-4461

P3 Primary Cell 4D-Nucleofector™ X Kit L V4XP-3012

P3 Primary Cell 4D-Nucleofector^® X Kit L V4XP-3024

P3 原代细胞 4D-Nucleofector® X 试剂盒 L V4XP-3024

P3 Primary Cell 4D-Nucleofector™ X Kit S V4XP-3032

P3 原代细胞 4D-Nucleofector™ X 试剂盒 S V4XP-3032

P3 Primary Cell 96-well Nucleofector™ Kit V4SP-3096

P3 Primary Cell 96-well Nucleofector™ Kit (960 RCT) V4SP-3960

P3 原代细胞 96 孔 Nucleofector™ 试剂盒 (960 RCT) V4SP-3960

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3002

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3002

P3 Primary Cell 384-well Nucleofector™ Kit V5SP-3010

P3 原代细胞 384 孔 Nucleofector™ 试剂盒 V5SP-3010

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