BioMag® & BioMag®Plus Binding Proteins

BioMag ®具有亲和结合蛋白的磁性颗粒可以通过直接孵育而被一抗或感兴趣的生物素化分子包被。它们也适用于现成的应用,例如免疫沉淀或 PCR 产物净化。

BIOMAG ®链霉亲和素 / 生物素

目录编号 描述 浓度(毫克/毫升) 体积(毫升)
BM551 BioMag® Streptavidin 5 5, 50
BP628 BioMag® Plus Streptavidin 5 10
BM568 BioMag® Streptavidin Nuclease-free 1 10, 25
BP621 BioMag® Plus Streptavidin/Biotin Binding Kit 5 5

参考

Sroka-Bartnicka A, Karlsson I, Ndreu L, Quaranta A, Pijnappel M, Thorsén G (2017)使用非糖基化结合剂对生物样品中选定蛋白质的基于粒子的 N-连接聚糖分析。J Pharm Biomed Anal, V.132 p125-132(BioMag 链霉亲和素)

磁粉样品包

我们的许多磁性颗粒产品独特地解决了生命科学中的广泛应用,包括细胞分离、亲和纯化、免疫测定、分子测定。对于开发工作,我们的新磁性粒子样品包允许您测试不同的粒子,以找到在您的特定系统中产生最佳性能特征的粒子。有关详细信息,请参阅PDS 749。

目录编号 描述
21940 Carboxyl Magnetic Sample Pack

MFY0002, Magnefy – 5mL (5% solids) 50mg/mL
PMC1N, ProMag Series 1 COOH – 5mL (2.5% solids) 25mg/mL
PMC3HP, ProMag HP 3μm COOH – 5mL (2.5% solids) 25mg/mL
BP618, BioMagPlus COOH – 5mL (2% solids) 20mg/mL

21950 Streptavidin Magnetic Sample Pack

MFYS1N, Magnefy 1µm SA – 1mL (1% solids)
PMS1N, ProMag 1 Series SA – 1mL (1% solids) 10mg/mL
PMS3HP, ProMag HP 3μm SA – 1mL (1% solids) 10mg/mL
BP628, BioMagPlus SA – 2mL (0.5% solids) 5mg/mL

BIOMAG ®蛋白质 A 和 G

Catalog Number Description Concentration (mg/mL) Volume (mL)
BM554 BioMag® Protein A 5 2, 10
BP620 BioMag® Plus Protein A 5 2, 10
BP614 BioMag® Plus Protein A Antibody Isolation Kit 5 2.5
BM553 BioMag® Protein G 5 2, 10
BP627 BioMag® Plus Protein G 5 2, 10
BP626 BioMag® Plus Protein G Antibody Isolation Kit 5 2.5

参考

Barnard, GC, Kull, AR, Sharkey, NS 等人 (2010)表达单克隆抗体的酵母细胞系的高通量筛选和选择J Ind Microbiol Biotechnol Vol 37(9)pp 961–971

BIOMAG ®二级抗体

BioMag ®二抗颗粒广泛用于捕获用于免疫沉淀的免疫复合物和用于间接细胞分离的抗体标记细胞。二抗涂层还提供了一种将特异性一抗与 BioMag ®颗粒结合的便捷方法。

Catalog Number Description Concentration (mg / mL) Volume (mL)
BM563 BioMag® Goat anti-Human IgG 1 50
BM562 BioMag® Goat anti-Human IgG (Fc Specific) 5 50
BM561 BioMag® Goat anti-Human IgM 1 50
BM549 BioMag® Goat anti-Mouse IgG 1 50 & 500
BM550 BioMag® Goat anti-Mouse IgG (Fc Specific) 5 50 & 500
BP619 BioMag® Plus Goat anti-Mouse IgG 1 50
BP612 BioMag® Plus Goat anti-Mouse IgG Antibody Coupling Kit 1 25
BM558 BioMag® Goat anti-Mouse IgM 1 50 & 500
BM559 BioMag® Goat anti-Rabbit IgG 1 50 & 500
BM560 BioMag® Goat anti-Rat IgG 1 50 & 500
BM548 BioMag® Goat anti-Rat IgG (Fc specific) 5 50 & 500
BM557 BioMag® Goat anti-Rat IgM 5 50 & 500

BIOMAG ®凝集素

目录编号 描述 浓度(毫克/毫升) 体积(毫升)
BP530 BioMag® Plus Wheat Germ Agglutinin 5 3, 10
BP531 BioMag® Plus Concanavalin A 5 3, 10

参考

杨,港龙,等。从肝细胞癌血清中选择性分离和分析糖蛋白组分及其糖组。蛋白质组学 13.9 (2013): 1481-1498。

Skene、彼得 J. 和史蒂文 Henikoff。“ CUT&RUN:针对低细胞数量的高效原位全基因组分析。 ” bioRxiv (2017): 193219. (BP531, BioMagPlus Con A)