荧光强度标准在仪器 QC 程序和生物分析中都有很多应用。我们为这些应用提供两种强度标准套件。我们的 Dragon Green 和 Flash Red Intensity Standard Kit 包含五组约 8 µm 聚苯乙烯基微球，它们各自的荧光团数量不断增加。
不同强度的群体可以作为荧光显微镜应用的相对强度标准，其中，作为内部染色的珠子，它们将经受住成像的严酷考验。珠子也可以作为流式细胞术的明亮相对强度或线性标准；如果您对此感兴趣，请咨询我们的QuickCal ®线性模板。
DRAGON GREEN INTENSITY STANDARD
The Dragon Green Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Dragon Green fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. Dragon Green is an excellent spectral surrogate for fluorescein, and is suitable for use with fluorescein filter sets. View excitation and emission spectra here.
Dragon Green Intensity Standard 是一个试剂盒，由五个单独的约 8 µm 聚苯乙烯基微球群组成，这些微球用越来越多的 Dragon Green 荧光团染色。每个种群在 1mL 瓶中以 ~1.5 x 10 7珠/mL 的价格供应。Dragon Green 是荧光素的极佳光谱替代品，适用于荧光素滤光片组。在此处查看激发和发射光谱。
| Catalog Number | Description | Product Data Sheet | Safety Data Sheet |
|---|---|---|---|
| DG06M | Dragon Green Intensity Standard (5 Intensities) | PDS 704.pdf | SDS FG006.pdf |
REFERENCES
Miao, Q, Rahn, Bryant J, R, Lancaster, C, Tourovskaia, A , Neumann, T ,… Nelson, A (2009) Multimodal three-dimensional imaging with isotropic high resolution using optical projection tomography. Proc. SPIE 7262, Medical Imaging : Biomedical Applications in Molecular, Structural, and Functional Imaging
Choi H, Kim KB, Jun C, Chung TD, Kim HC (2012) A portable microfluidic chip system for cancer diagnosis with simultaneous detection methods. Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85484A
Mao X, Lin SS, Dong C, Huang T (2009) Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. Lab Chip, 2009,9, 1583-1589
FLASH RED INTENSITY STANDARD
The Flash Red Intensity Standard is a kit consisting of five individual populations of ~8µm polystyrene-based microspheres dyed with increasing amounts of the Flash Red fluorophore. Each population is supplied at ~1.5 x 107 beads /mL in a 1mL bottle. As Flash Red is spectrally similar to Cy™5, traditional red fluorophore filter sets (e.g. Cy™5/microscope, PE-Cy™5 or APC/cytometer) may be used with this standard. View excitation and emission spectra here.
Flash Red Intensity Standard 是一个试剂盒，由五个单独的约 8 µm 聚苯乙烯基微球组成，这些微球被越来越多的 Flash Red 荧光团染色。每个种群在 1mL 瓶中以 ~1.5 x 10 7珠/mL 的价格供应。由于 Flash Red 在光谱上与 Cy™5 相似，因此传统的红色荧光团滤光片组（例如 Cy™5/显微镜、PE-Cy™5 或 APC/细胞仪）可以与该标准一起使用。在此处查看激发和发射光谱。
| Catalog Number | Description | Product Data Sheet | Safety Data Sheet |
|---|---|---|---|
| FR06M | Flash Red Intensity Standard (5 Intensities) | PDS 704.pdf | SDS FG006.pdf |
REFERENCES
Meineke, G, Flitsch, D , Lenenbach, A , Noll, R (2013) Fluorescence sensors for parallel measurements in multichannel microfluidic devices covering the full channel cross sections. Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI