抗HA磁珠

抗HA磁珠是亲和颗粒，可使用手动或自动磁选机免疫沉淀在细菌或哺乳动物细胞或体外系统中表达的重组HA标签蛋白质。 -HA单克隆抗体（克隆2-2.2.14）可实现高产量和高纯度的免疫沉淀。特异性结合-稳定，预封闭的磁珠和特异性抗体可最大程度地减少HA标签IP或co-IP实验的脱靶结合。|•多功能-磁珠与手动和自动工作流程兼容。抗HA抗体，一种高度特异性的小鼠IgG1单克隆抗体，可识别源自人流感血凝素（HA）蛋白的HA表位标签（YPYDVPDYA）。Anti-HA Magnetic Beads既可以在磁力架上手

Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.|Features of Anti-HA Magnetic Beads:|• Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation.|• Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour.|• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments.|• Versatile—beads are compatible with manual and automated workflows.|The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms.|Product Details:|Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0), 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL of HA peptide can be used.
•Do not centrifuge, dry or freeze Anti-HA Magnetic Beads. Centrifuging, drying or freezing will cause thebeads to aggregate and lose binding activity. To ensure good dispersal of beads for optimal antibody binding, it is important to include 0.025% to 0.1
Anti-HA Magnetic Beadsis for immunoprecipitation of recombinant HA-tagged proteins