Pricing & Availability
- Clone
- 10.1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI MA36
- Other Names
- FcγRI, FcR I
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats See APC/Cyanine7 spectral data
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Cat # | Size | Price | Quantity Check Availability | |||
---|---|---|---|---|---|---|
305025 | 25 tests | $125.00 | ||||
305026 | 100 tests | $305.00 |
DescriptionCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
CD64 是一种 72 kD 的单链 I 型糖蛋白,也称为 FcγRI 和 FcR I。CD64 是免疫球蛋白超家族的成员,在单核细胞/巨噬细胞、树突状细胞和活化的粒细胞上表达。 表达可以通过干扰素γ 刺激上调。 CD64 结合 IgG 免疫复合物。 它在抗原捕获、IgG/抗原复合物的吞噬作用和抗体依赖性细胞毒性 (ADCC) 中发挥作用。
Product Details
Technical data sheet
Product Details
- Reactivity
- Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- FC – Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
- Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact if you have any questions.
- 应用笔记
克隆 10.1 识别 CD64 的 EC3 表位。 虽然两者都包含 EC3 结构域,但内部测试表明克隆 10.1 优先结合 CD64A (FcγRIA),而不是 CD64B (FcγRIB)。 其他报告的应用(针对相关格式)包括:阻断人 IgG3 和鼠 IgG2a 与 FcγRI2、5、6、11 的结合以及丙酮固定冷冻组织切片的免疫组织化学染色 12。附加产品说明
BioLegend 正在将名称 APC/Cy7 转换为 APC/Cyanine7。 染料分子保持不变,因此您应该期望我们的 APC/Cyanine7 产品具有相同的质量和性能。 如果您有任何问题,请联系。 - Application References(PubMed link indicates BioLegend citation)
-
- McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Holl V, et al. 2004. J. Immunol. 173:6274.
- Hober D, et al. 2002. J. Gen. Virol. 83:2169.
- Cho HJ, et al. 2007. Physiol Genomics 149:60.
- van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
- Bruhns P, et al. 2008. Blood 113:3716. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Carter DL, et al. 1999. Cytometry 37:41. (FC)
- Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
- Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
- Product Citations
-
- Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
- Santra S, et al. 2015. PLoS Pathog. 11: 1005042. PubMed
- Quast I, et al. 2015. Neurol Neuroimmunol Neuroinflamm. 2: e148. PubMed
- RRID
- AB_2561587 (BioLegend Cat. No. 305025)
AB_2561588 (BioLegend Cat. No. 305026)
Antigen Details
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
- Monocytes, macrophages, dendritic cells, activated granulocytes
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG receptor
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
- 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215. - Gene ID
- 2209 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
Related FAQs
- Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
- Yes
Other Formats
View All CD64 Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
Biotin anti-human CD64 | 10.1 | FC |
FITC anti-human CD64 | 10.1 | FC |
PE anti-human CD64 | 10.1 | FC |
Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
Alexa Fluor® 488 anti-human CD64 | 10.1 | FC |
Alexa Fluor® 647 anti-human CD64 | 10.1 | FC |
APC anti-human CD64 | 10.1 | FC |
Pacific Blue™ anti-human CD64 | 10.1 | FC |
Brilliant Violet 421™ anti-human CD64 | 10.1 | FC |
PE/Cyanine7 anti-human CD64 | 10.1 | FC |
PerCP/Cyanine5.5 anti-human CD64 | 10.1 | FC |
APC/Cyanine7 anti-human CD64 | 10.1 | FC |
Brilliant Violet 510™ anti-human CD64 | 10.1 | FC |
Purified anti-human CD64 (Maxpar® Ready) | 10.1 | FC, CyTOF® |
PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
Brilliant Violet 605™ anti-human CD64 | 10.1 | FC |
APC/Fire™ 750 anti-human CD64 | 10.1 | FC |
PE anti-human CD64 | 10.1 | FC |
PE/Dazzle™ 594 anti-human CD64 | 10.1 | FC |
FITC anti-human CD64 | 10.1 | FC |
TotalSeq™-A0162 anti-human CD64 | 10.1 | PG |
Brilliant Violet 711™ anti-human CD64 | 10.1 | FC |
Alexa Fluor® 700 anti-human CD64 | 10.1 | FC |
Brilliant Violet 785™ anti-human CD64 | 10.1 | FC |
TotalSeq™-C0162 anti-human CD64 | 10.1 | PG |
Ultra-LEAF™ Purified anti-human CD64 | 10.1 | FC, IHC-F, Block |
TotalSeq™-B0162 anti-human CD64 | 10.1 | PG |
TotalSeq™-D0162 anti-human CD64 | 10.1 | PG |
GMP PE anti-human CD64 | 10.1 | FC |
GMP FITC anti-human CD64 | 10.1 | FC |