Precaution♦ 使用本试剂盒标记的蛋白质的分子量应大于 50,000。♦ 标记过程中，IgG 或荧光素结合的 IgG 始终在过滤管的膜上。♦ 如果 IgG 溶液中含有其他具有分子量的蛋白质 大于 10,000，例如 BSA 或明胶，在使用该试剂盒标记荧光素之前纯化 IgG 溶液。 IgG 溶液可通过 IgG 纯化试剂盒（本试剂盒未包含）进行纯化。♦ 如果 IgG 溶液含有少量不溶性物质，则将溶液离心并使用上清液进行标记。
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Yes, if the molecular weight is greater than 50,000.
If the protein solution does not contain small molecules with an amino group and the concentration of the protein is 10 mg per ml, or about 70 μM, there is no need to use the Filtration tube. Just mix 10 μl of the sample solution with 90 μl of Reaction buffer and add 8 μl NH2-reactive fluorescein (prepared at step 3) to the mixture, and follow the protocol starting at step 4.
If you store at 4ºC, it is stable for over 2 months. For longer storage, add 100% volume of glycerol, aliquot, and store at -20ºC. However, please note that stability depends on the protein itself.
The minumum amount of IgG is 10 μg; simply follow the protocol. The labeling ratio remains the same for 10 μg to 100 μg of IgG.
No. Oligonucleotides and peptides may be too small to retain on the membrane filter of the Filtration tube.