biolegend流式抗体-Ultra-LEAF™ Purified anti-human CD64 Antibody

Pricing & Availability

Clone

10.1 (See other available formats)

Regulatory Status

RUO

Workshop

VI MA36

Other Names

FcγRI, FcR I

Isotype

Mouse IgG1, κ

Ave. Rating

Submit a Review

Product Citations

publications

• 

Compare all formats

Technical data sheet

Product Details

Reactivity

Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.

Storage & Handling

Application

FC – Quality tested
IHC-F, Block – Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis

Application Notes

Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².

Application References

(PubMed link indicates BioLegend citation)

1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. Holl V, et al. 2004. J. Immunol. 173:6274.
5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)

Product Citations

1. Gruber CN, et al. 2020. Cell. 183:982. PubMed

RRID

AB_2810455 (BioLegend Cat. No. 305047)
AB_2810456 (BioLegend Cat. No. 305048)

Antigen Details

Structure

Ig superfamily, type I glycoprotein, 72 kD

Distribution

Monocytes, macrophages, dendritic cells, activated granulocytes

Function

Phagocytosis, ADCC

Ligand/Receptor

IgG receptor

Cell Type

Dendritic cells, Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, Fc Receptors

Antigen References

1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.

Gene ID

2209 View all products for this Gene ID

UniProt

View information about CD64 on UniProt.org

Related Products

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Do you guarantee that your antibodies are totally pathogen free?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD64 Reagents Request Custom Conjugation