biolegend流式抗体-APC anti-mouse CD45.2 Antibody

Pricing & Availability

Clone

104 (See other available formats)

Regulatory Status

RUO

Other Names

Ly-5.2, LCA

Isotype

Mouse (SJL) IgG2a, κ

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Product Citations

publications

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Technical data sheet

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC – Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³. 

Application References

(PubMed link indicates BioLegend citation)

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

1. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
2. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
3. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
4. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
5. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
6. Xu X, et al. 2018. Cell. 175:1336. PubMed
7. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
8. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
9. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
10. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
11. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
12. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
13. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
14. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
15. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
16. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
17. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
18. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
19. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
20. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
21. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
22. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
23. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
24. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
25. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
26. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
27. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
28. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
29. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
30. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
31. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
32. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
33. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
34. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
35. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
36. Markey K, et al. 2012. Blood. 119:5918. PubMed
37. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
38. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
39. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
40. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
41. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
42. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
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44. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 – 13080. PubMed
45. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
46. Kondo M, et al. 2016. J Immunol. 196: 563 – 572. PubMed
47. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
48. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
49. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
50. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
51. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
52. Boyd DF, et al. 2020. Nature. 587:466. PubMed
53. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
54. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
55. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
56. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
57. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
58. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
59. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
60. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
61. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
62. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
63. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
64. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
65. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
66. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
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69. Marangoni F, et al. 2021. Cell. . PubMed

RRID

AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules

Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45.2 on UniProt.org

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Other Formats

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