Cryopreserved ampule of Normal Human Lung Fibroblasts (NHLF) containing ≥ 500,000 cells
Product Overview
Lonza’s Human Lung Fibroblasts are isolated from adult lung tissue. Some matched donor sets of lung fibroblasts, bronchial smooth muscle cells, bronchial epithelial cells may be available. Contact Customer Service or Scientific Support for more information.
Lung fibroblasts play a role in airway inflammation and airway remodeling. They can be a target for asthma medications in pharmaceutical development. The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa.
Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. Important research applications includ pulmonary disease, i.e. COPD, asthma, CF, TB, cancer.
Cryopreserved CloneticsTM Normal Human Lung Fibroblasts cells are guaranteed through 15 population doublings, they are tested for negative von Willebrand Factor Expression/Factor VIII, cytokeratins 18 and 19, and alpha smooth muscle actin All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Growth Media (catalog no. CC-3132)
Lonza 的人肺成纤维细胞是从成人肺组织中分离出来的。一些匹配的肺成纤维细胞、支气管平滑肌细胞、支气管上皮细胞的供体组可能是可用的。有关更多信息，请联系客户服务或科学支持。
肺成纤维细胞在气道炎症和气道重塑中发挥作用。它们可以成为药物开发中哮喘药物的目标。肺间质中最丰富的细胞类型是成纤维细胞。它们类似于普通的成纤维细胞，但具有一些显着特征，例如，它们具有长分支过程和间隙连接。它们的主要功能是产生肺泡隔细胞外基质的 III 型胶原蛋白、弹性蛋白和蛋白聚糖。
肺成纤维细胞 (PF) 在损伤后的修复和重塑过程中发挥重要作用。成纤维细胞在炎症部位的受控积累对于损伤后的有效组织修复至关重要。成纤维细胞的不充分或过度积累都可能导致组织功能异常。例如，成纤维细胞的过度增殖导致在缺氧诱导的肺动脉高压发展过程中观察到的外膜增厚。重要的研究应用包括肺部疾病，即 COPD、哮喘、CF、TB、癌症。
冷冻保存的 CloneticsTM 正常人肺成纤维细胞保证通过 15 次群体倍增，它们被测试为阴性 von Willebrand 因子表达/因子 VIII、细胞角蛋白 18 和 19，以及 α 平滑肌肌动蛋白 所有细胞的支原体、细菌、酵母和真菌测试均为阴性.并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。
购买我们的肺成纤维细胞培养试剂盒（目录号 CC-2512B）并获得细胞和培养基的折扣价。该试剂盒由冷冻保存的肺成纤维细胞安瓿（货号 CC-2512）和 FGMTM-2 BulletKitTM 生长培养基（货号 CC-3132）组成
Content & Storage
Content
1 x Cryopreserved ampule of NHLF containing ≥ 500,000 cells
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NHLF – Human Lung Fibroblasts
Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was Horse anti Mouse, DI 2488 VectorLabs, used at 1:200 dilution. Nuclei were stained with Hoechst at 1:10,000.
NHLF – Human Lung Fibroblasts
Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was , Goat anti Chicken Alexa 594, A11042 Invitrogen: Used at 1:200, Horse anti Mouse, DI 2488 VectorLabs: Used at 1:200 Nuclei were stained with Hoechst.
