含有 ≥ 500,000 个细胞的正常人肺动脉内皮细胞 (HPAEC) 冻存安瓿
Product Overview
Lonza’s Human Pulmonary Artery Endothelial Cells (HPAEC) and media are convenient and easy to use, allowing the researcher to focus on results. Cryopreserved HPAEC cells are shipped in third passage. Proliferating cells are shipped in fourth passage. Cells isolated from Diabetes Type 1 and 2 are also available.
CloneticsTM cells, medium and reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.
Lonza 的人肺动脉内皮细胞 (HPAEC) 和培养基使用方便且易于使用，使研究人员能够专注于结果。 冷冻保存的 HPAEC 细胞在第三代运输。 增殖细胞在第四代运输。 也可使用从 1 型和 2 型糖尿病中分离的细胞。
CloneticsTM 细胞、培养基和试剂一起经过质量测试，保证作为一个完整的细胞系统提供最佳性能。
Benefits
- Assured for experimental use up to 15 population doublings
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Characterized by morphological observation throughout serial passage
- Certificate of Analysis provided for every cell lot
Applications
- Hypertension
- Arteriosclerosis
- Inflammation
- Oncology
- Drug screening
Content & Storage
Content
1 x Cryopreserved ampule of HPAEC containing ≥ 500,000 cells
D-PASMC – 患病的人肺动脉平滑肌细胞 – II 型糖尿病 CC-2913
HPAEC – Human Pulmonary Artery Endothelial Cells
Human pulmonary artery endothelial cells (CC-2530) were immunohistochemistry labeled with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1088 DyLight 488 from (Vector Laboratories). Staining for CD31/PECAM-1 was conducted using antibody from R&D Systems, BBA7 at 1:100. Secondary antibody used to detect CD31/PECAM-1 was VECTDI2594 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst.
HPAEC – Human Pulmonary Artery Endothelial Cells
Human pulmonary artery endothelial cells (CC-2530) were immunohistochemistry labeled with von Willebrand Factor antibody (Sigma, F3520) used at 1:200. Secondary antibody used was VECTDI1088 DyLight 488 from (Vector Laboratories). Staining for CD31/PECAM-1 was conducted using antibody from R&D Systems, BBA7 at 1:100. Secondary antibody used to detect CD31/PECAM-1 was VECTDI2594 DyLight 594 (Vector Laboratories). Nuclei were stained with Hoechst.
用 1:200 使用的血管性血友病因子抗体 (Sigma, F3520) 对人肺动脉内皮细胞 (CC-2530) 进行免疫组织化学标记。 使用的二抗是来自 (Vector Laboratories) 的 VECTDI1088 DyLight 488。 使用来自 R&D Systems 的抗体 BBA7 以 1:100 进行 CD31/PECAM-1 染色。 用于检测 CD31/PECAM-1 的二抗是 VECTDI2594 DyLight 594 (Vector Laboratories)。 细胞核用赫斯特染色。