Cryopreserved ampule of Normal Human Ventricular Cardiac Fibroblasts (NHCF-V) containing ≥ 500,000 cells
Product Overview
Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle. Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2.
CloneticsTM NHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. CloneticsTM AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
人类心脏相关成纤维细胞包括从升主动脉或降主动脉外部的被膜分离的主动脉外膜成纤维细胞 (AoAF) 和从心房或心室分离的心脏成纤维细胞 (NHCF-A 和 -V)。 细胞是从正常的成人心脏组织中分离出来的。 冷冻保存的细胞在第 2 代运送。
CloneticsTM NHCF-V 和 -A 通过 5 次群体倍增得到保证,解冻后表达 ≥90% 的胶原蛋白 I 和 ≤10% 的 Von Willibrand 因子 VIII。 CloneticsTM AoAF 通过 10 次群体倍增得到保证,并且对 α 平滑肌肌动蛋白呈阴性。 所有细胞的支原体、细菌、酵母菌和真菌均呈阴性。 并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。 为购买的每个细胞批次提供分析证书 (CoA)。
Benefits
- Assured for experimental use up to 15 population doublings
- Express ≥90% collagen I and ≤10% Von Willibrand factor VIII
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Applications
- Vascular diseases
- Neointimal formation
- Vascular remodeling
- Atherosclerosis
- Hypertension
- Myocardial repair
- Extracellular maintenance during cardiac cell / tissue growth
- Cardiac Fibrosis Paracrine signalling
- Toxicology
- Gene expression
Content & Storage
Content
1 x Cryopreserved ampule NHCF-V containing ≥ 500,000 cells/vial
HAEC – 人主动脉内皮细胞 CC-2535
AoSMC – 人主动脉平滑肌细胞 CC-2571