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Product Overview

Natural Killer (NK) cells are lymphocytes of the immune system that are critical in host defense and immune regulation as part of both the innate and adaptive immune response. They are of particular interest to immunotherapy researchers as they hold the promise to specifically target and destroy cancer cells and tumors while leaving healthy cells intact. Cells You Can Count on to Perform Natural Killer cells are traditionally characterized by the presence of the CD56 cell surface marker and absence of CD3. In addition, CD16 is important for NK cell function because CD16 induces cytokine expression and cytotoxic effector activity. Lonza NK cells are isolated using positive (2W-502) or negative (2W-501) immunomagnetic selection for the CD56 marker. Purity is ≥90% based on the presence of CD56+ cells. Both positively and negatively selected NK cells are guaranteed to express CD56 and CD16. However, negative immunomagnetic separation of NK cells results in significantly greater enrichment of CD16+ cells (typically > 80% of total cells), whereas expression of CD16 is variable with the positively selected NK cells. Lonza NK cells are guaranteed to express CD56 and CD16, and contain ≥5 million viable cells per vial. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Choices in Cell Type and Tissue Source Expression of CD56 and CD16 can greatly influence cell function. In addition, selection techniques can also influence function of your target cells. That’s why Lonza offers both positively and negatively selected NK cells and characterizes the expression levels of both CD56 and CD16 in every lot of cells. We also offer choices in tissue source if peripheral blood derived natural killer cells aren’t what you’re looking for. Cord blood MNCs or bone marrow MNCs can be depleted of lineage marker expressing cells in order to isolate NK cells. In addition, cord blood CD34+ cells or bone marrow CD34+ cells can be differentiated into natural killer cells. Supported by Lonza Medium LGM-3^TM Lymphocyte Growth Medium is recommended for optimal performance of NK cells. Additional cytokines may be necessary depending on application. Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

自然杀伤 (NK) 细胞是免疫系统的淋巴细胞，作为先天性和适应性免疫反应的一部分，对宿主防御和免疫调节至关重要。它们对免疫治疗研究人员特别感兴趣，因为它们有望专门针对和破坏癌细胞和肿瘤，同时保持健康细胞完好无损。您可以依靠的细胞来执行自然杀伤细胞的传统特征是存在 CD56 细胞表面标志物且不存在 CD3。此外，CD16 对 NK 细胞功能很重要，因为 CD16 诱导细胞因子表达和细胞毒效应活性。使用 CD56 标记的​​阳性 (2W-502) 或阴性 (2W-501) 免疫磁性选择分离 Lonza NK 细胞。基于 CD56+ 细胞的存在，纯度≥90%。阳性和阴性选择的 NK 细胞都保证表达 CD56 和 CD16。然而，NK 细胞的负免疫磁性分离导致 CD16+ 细胞的富集显着增加（通常 > 总细胞的 80%），而 CD16 的表达随阳性选择的 NK 细胞而变化。 Lonza NK 细胞保证表达 CD56 和 CD16，每瓶含有 ≥500 万个活细胞。并非所有供体和/或细胞批次都检测到 HIV-1、乙型肝炎和丙型肝炎。为购买的每个细胞批次提供分析证书。 CD56和CD16的细胞类型和组织来源表达的选择可以极大地影响细胞功能。此外，选择技术也会影响目标细胞的功能。这就是为什么 Lonza 提供阳性和阴性选择的 NK 细胞，并表征每批细胞中 CD56 和 CD16 的表达水平。如果外周血衍生的自然杀伤细胞不是您想要的，我们还提供组织来源选择。脐带血 MNC 或骨髓 MNC 可以去除谱系标记表达细胞，以分离 NK 细胞。此外，脐带血 CD34+ 细胞或骨髓 CD34+ 细胞可分化为自然杀伤细胞。由 Lonza 培养基支持 LGM-3TM 淋巴细胞生长培养基推荐用于 NK 细胞的最佳性能。根据应用，可能需要额外的细胞因子。只有在专门使用适当的 CloneticsTM/PoieticsTM 培养基和试剂并遵循推荐的储存和使用协议时，Lonza 才能保证 CloneticsTM/PoieticsTM 细胞的性能。对推荐的细胞系统进行的任何修改，包括使用替代培养基、试剂或方案，都将使细胞和培养基性能保证失效。

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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