Reagents > IRDye® 680LT Goat anti-Mouse IgG2b-Specific Secondary Antibody
Immunogen
Mouse IgG2b paraproteins
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG2b covalently linked to agarose. Based on ELISA and flow cytometry, the antibody reacts with the heavy chain of mouse IgG2b. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.
Applications
Recommended for:
- Western Blot
- Protein Array
- Immunohistochemistry
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
Not Recommended for:
- Small Animal Imaging
- In-Cell Western Assay
- On-Cell Western Assay
Formulation
IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey Western blot detection | 1:20,000 | 1:20,000 – 1:50,000 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 926-68052: RRID AB_2783644
Example Data
