LI-COR IRDye® 800CW 羧酸盐,IRDye 800CW

Reagents > IRDye® 800CW Carboxylate,IRDye® 800CW 羧酸盐

IRDye® 800CW Carboxylate

Assays that use IRDye 800CW conjugates (such as in vivo imaging and cell binding assays) may require a “dye-only” control for potential effects or retention of the dye.

Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.

使用 IRDye 800CW 偶联物的检测（例如体内成像和细胞结合检测）可能需要“仅染料”对照来控制染料的潜在影响或保留。

IRDye 800CW 的羧酸盐（非反应性）形式是理想的对照。

Note: The carboxylate dye has no reactive group and cannot be used for labeling.

IRDye® 800CW Carboxylate
Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly and was completely cleared after 48 h. Originally published in Kovar, J., et al. (2007) Anal Biochem 367:1-12.
IRDye 800CW 羧酸盐的清除动力学。 给一只 SCID 小鼠注射 1 nmol 的非反应性 IRDye 800CW 染料，并如图所示随时间监测清除率。 伪彩色荧光叠加在白光图像上。 IRDye 800CW 羧酸盐迅速分散，48 小时后完全清除。 最初发表于 Kovar, J. 等人。 (2007) 肛门生物化学 367:1-12。

How is IRDye 800CW Carboxylate Used?

In vitro Cell-based Assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

  • Validation of optical agents for in vivo administration
  • Evaluation of binding specificity

In vivo Imaging

For evaluation of behavior and clearance of the dye itself.

  • Timing of dye clearance from the animal’s body
  • Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted (“free”) dye after IRDye 800CW conjugation and purification.

Residual unreacted dye may cause:

  • Artificially high values when dye/protein (D/P) ratio is calculated
  • Increased background fluorescence in biological assays

Dye Structure

IRDye® 800CW Carboxylate

Properties

  • Chemical Formula: C46H50N2Na4O15S4
  • Molecular Weight: 1091.10 g/mol
  • Exact Mass: 1090.17

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