Reagents > IRDye® 800CW Donkey anti-Goat IgG Secondary Antibody, IRDye® 800CW 驴抗山羊 IgG 二抗
Immunogen
Goat IgG, whole molecule
Purity and Specificity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on electrophoresis, this antibody reacts with the heavy and light chains of goat IgG, and with the light chains common to most goat immunoglobulins. This antibody also reacts with sheep IgG. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with chicken. guinea pig, Syrian hamster, horse, human, mouse, rat, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
使用与琼脂糖珠偶联的抗原，通过免疫亲和层析从抗血清中分离抗体。 基于电泳，该抗体与山羊 IgG 的重链和轻链以及大多数山羊免疫球蛋白共有的轻链发生反应。 该抗体还与绵羊 IgG 发生反应。 未检测到针对非免疫球蛋白血清蛋白的抗体。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与鸡的交叉反应最小。 豚鼠、叙利亚仓鼠、马、人、小鼠、大鼠和兔血清蛋白，但可能与其他物种的免疫球蛋白发生交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。
Applications
Highly recommended for:
- Western Blot
- In-Cell Western™ Assay
- On-Cell Western Assay
- Protein Array
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
Note: Do not use this secondary antibody in combination with any secondary antibody whose host is goat (for example, IRDye 680RD Goat anti-Rabbit) for two-color Western blot detection.
Formulation
IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey® Western blot detection | 1:15,000 | 1:5,000 – 1:25,000 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-32214: RRID AB_2687553
- P/N 926-32214: RRID AB_621846