LI-COR IRDye® 800CW 驴抗豚鼠 IgG 二抗,In-Cell Western™ 检测

Immunogen

Guinea Pig IgG, whole molecule

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.

使用固定在琼脂糖珠上的抗原通过亲和层析实现特异性抗体的分离。 基于免疫电泳和/或 ELISA，该抗体与全分子豚鼠 IgG 以及其他豚鼠免疫球蛋白共有的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的反应性。 该抗体已通过 ELISA 和/或固相吸附测试，以确保与牛、鸡、山羊、叙利亚仓鼠、马、人、小鼠、兔、大鼠和绵羊血清蛋白的交叉反应最小。 该偶联物经过专门测试，可用于蛋白质印迹和 In-Cell Western™ 检测应用。

Applications

Highly recommended for:

• Western Blot
• In-Cell Western Assay
• On-Cell Western Assay
• Protein Array
• Immunohistochemistry
• Small Animal Imaging
• Microscopy
• 2D Gel Detection
• Tissue Section Imaging
• Virus Titration Assay
• 蛋白质印迹
  细胞内西方检测
  On-Cell Western 检测
  蛋白质阵列
  免疫组织化学
  小动物成像
  显微镜
  2D 凝胶检测
  组织切片成像
  病毒滴定法

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

IRDye 800CW 二抗以纯化的免疫球蛋白偶联物形式提供，在 pH 7.4 的磷酸盐缓冲盐水中冻干。 避光。 在重组前储存在 4°C。

复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

RRID

• P/N 925-32411: RRID AB_2814905
• P/N 926-32411: RRID AB_1850024

Example Data