LI-COR IRDye® QC-1 NHS 酯,IRDye 800CW 配对用于光声

Reagents > IRDye® QC-1 NHS Ester,IRDye® QC-1 NHS 酯

IRDye® QC-1 NHS Ester

IRDye QC-1 is the first non-fluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500-800 nm). It has the widest available quenching range, so you do not need to carefully match the donor’s fluorescence spectrum with the acceptor’s absorbance. IRDye QC-1 quenches common fluorophores with >97% efficiency1.

IRDye QC-1 是第一个与各种可见光和近红外荧光团（~500-800 nm）兼容的非荧光（暗）猝灭剂。 它具有最宽的可用猝灭范围，因此您无需仔细匹配供体的荧光光谱与受体的吸光度。 IRDye QC-1 以 >97% 的效率淬灭常见的荧光团1。

IRDye® QC-1 NHS Ester
Energy that is transferred from the donor (Dem) can be emitted at the acceptor’s wavelength (Aem), quenching fluorescence.

For more information, see “Spectral Overlap of IRDye QC-1 Absorption with Common Donor Fluorophores” below.

IRDye QC-1 NHS ester can be used for FRET (fluorescence resonance energy transfer) applications such as protease assays. Recently, it has been paired with IRDye 800CW in optoacoustic studies.

有关更多信息，请参阅下面的“IRDye QC-1 吸收与常见供体荧光团的光谱重叠”。

IRDye QC-1 NHS 酯可用于 FRET（荧光共振能量转移）应用，例如蛋白酶分析。 最近，它已与 IRDye 800CW 配对用于光声研究。

IRDye Infrared Dyes Overview

Dye Structure

IRDye® QC-1 NHS Ester

Properties

  • Chemical Formula: C53H62C|N4Na3O16S4
  • Molecular Weight: 1243.75 g/mol
  • Exact Mass: 1242.24

Absorption Spectrum

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima
1X PBS 96,000 737 nm
Water 98,000 788 nm

Absorption Spectrum in 1X PBS

IRDye® QC-1 NHS Ester

Absorption Spectrum in Methanol

IRDye® QC-1 NHS Ester

Spectral Overlap of IRDye QC-1 Absorption with Common Donor Fluorophores

IRDye® QC-1 NHS Ester
Normalized emission spectra for several common donor fluorophores, and the absorption spectrum of IRDye QC-1. All are quenched by IRDye QC-1 with >97% efficiency. Reprinted with permission from Analytical Biochemistry 388 (2009) 220-28.
IRDye® QC-1 NHS Ester
List of fluorophores and spectrally compatible quenchers (shaded box = compatible). Almost all of these common fluorophores are spectrally compatible with IRDye QC-1. No other quencher spans this wide range or is compatible with wavelengths near 800 nm. Reprinted with permission from Analytical Biochemistry 388 (2009) 220-28.

Reference

  1. Peng, X et al. A nonfluorescent, broad range quencher dye for Fõrster resonance energy transfer assays. Anal Biochem. 388(2):220-28 (2009).

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