Reagents > IRDye® 680LT Donkey anti-Goat IgG Secondary Antibody,IRDye® 680LT 驴抗山羊 IgG 二抗
Immunogen
Goat IgG
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using goat IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with human, mouse, rabbit, rat, chicken. guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
使用与琼脂糖共价连接的山羊 IgG 通过亲和层析实现特异性抗体的分离。 基于 ELISA，该抗体与山羊 IgG 和绵羊 IgG 的重链和轻链反应。 该抗体已通过斑点印迹和/或固相吸附测试，以确保与人、小鼠、兔、大鼠、鸡的交叉反应最小。 豚鼠、仓鼠、猪和马血清蛋白，但可能与其他物种的免疫球蛋白发生交叉反应。 该偶联物经过专门测试，可用于蛋白质印迹应用。
Applications
Recommended for:
- Western Blot
- Protein Array
- Immunohistochemistry
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
Not Recommended for:
- Small Animal Imaging
- In-Cell Western Assay
- On-Cell Western Assay
Note: Do not use this secondary antibody in combination with any secondary antibody whose host is goat (for example, IRDye 800CW Goat anti-Rabbit) for two-color Western blot detection.
Formulation
IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
IRDye 680LT 二抗以纯化的免疫球蛋白偶联物形式提供，在磷酸盐缓冲盐水中冻干，pH 7.4。 避光。 在重组前储存在 4°C。
复溶后，每瓶含有 10 mg/mL BSA（不含 IgG 和蛋白酶）作为稳定剂和 0.01% 叠氮化钠作为防腐剂。 按照指示重新配制时，浓度为 1.0 mg/mL。 有关重组的详细信息，请参阅包装插页。
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey Western blot detection | 1:20,000 | 1:20,000 – 1:50,000 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-68024: RRID AB_2814908
- P/N 926-68024: RRID AB_10706168
Example Data
