Reagents > Revert™ 520 Total Protein Stain Kits for Western Blot Normalization,用于蛋白质印迹标准化的 Revert™ 520 总蛋白染色试剂盒
Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment.
使用 Revert 520 Total Protein Stain 使蛋白质印迹标准化更加准确和可靠，这是一种基于膜的（转移后）标准化策略，可对样品中的所有蛋白质进行染色。 Revert 520 Total Protein Stain 适用于您现有的蛋白质印迹工作流程，不需要特殊的凝胶或设备。
Use the Gold Standard for Western Blot Normalization
Total protein staining is considered the gold standard for Western blot normalization. Revert 520 Total Protein Stain provides linear signal over a broad range of sample concentrations and is compatible with subsequent Western blot immunodetection methods.
- Accurate Normalization: With a wide linear range, it’s easy to detect Revert 520 stain and your target in the same linear range for accurate normalization.
- Quick and Compatible: Stain total protein in less than ten minutes on either PVDF or nitrocellulose membranes.
- Reliable Analysis: Unlike housekeeping proteins, biological variation won’t affect total protein normalization with Revert 520 Total Protein Stain.
- 总蛋白染色被认为是蛋白质印迹标准化的金标准。 Revert 520 Total Protein Stain 在广泛的样品浓度范围内提供线性信号，并与随后的蛋白质印迹免疫检测方法兼容。
准确归一化：线性范围宽，很容易检测 Revert 520 染色剂和您的目标在相同的线性范围内进行准确归一化。
快速且兼容：在 PVDF 或硝酸纤维素膜上染色总蛋白不到 10 分钟。
可靠的分析：与管家蛋白质不同，生物变异不会影响使用 Revert 520 Total Protein Stain 的总蛋白质标准化。
Kit Components
The 200 mL Kit (P/N 926-10010) is enough for 20 mini blots and contains:
- 1 x 200 mL Revert 520 Total Protein Stain
- 1 x 400 mL Revert 520 Wash Solution
- 1 x 400 mL Revert Destaining Solution
The 500 mL Kit (P/N 926-10016) is enough for 50 mini blots and contains:
- 1 x 500 mL Revert 520 Total Protein Stain
- 1 x 1 L Revert 520 Wash Solution
- 1 x 1 L Revert Destaining Solution