LI-COR P/N 926-42215,Vinculin 兔单克隆抗体,LI-COR Vinculin 一抗

Reagents > Vinculin Rabbit Monoclonal Antibody for Normalization,用于标准化的 Vinculin 兔单克隆抗体

Vinculin Rabbit Monoclonal Antibody for Normalization

Vinculin is a highly conserved cytoskeletal protein. The Vinculin primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting high molecular weight targets.

The expression of Vinculin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, Vinculin primary antibodies can be used for the detection of Vinculin when performing multiplex Western blot detection.

Detect Vinculin Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

Vinculin 是一种高度保守的细胞骨架蛋白。 Vinculin 一抗可用作标准化的内部上样对照，在检测高分子量目标时特别有效。

应验证 Vinculin 或任何管家蛋白 (HKP) 的表达，以确保其表达在实验条件下不会改变。

一旦通过验证，在进行多重蛋白质印迹检测时，Vinculin 一抗可用于检测 Vinculin。

使用 IRDye® Goat anti-Rabbit 或 IRDye Donkey anti-Rabbit 二抗检测 Vinculin Rabbit Monoclonal Antibody。

管家蛋白质标准化的其他选择

Reactivity and Specificity

Vinculin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties Vinculin Rabbit Monoclonal Antibody (P/N 926-42215)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 124 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total vinculin protein and metavinculin, a 145 kDa splice variant of vinculin
Immunogen A recombinant protein that is specific to the amino terminus of human vinculin protein
Tested Application Western blot (WB), Immunohistochemistry (IHC), Flow Cytometry (F)

RRID

  • P/N 926-42215: RRID AB_2814925

Vinculin Rabbit Monoclonal Antibody Detected in C32 Cells

Vinculin Rabbit Monoclonal Antibody for Normalization
Vinculin Rabbit Monoclonal Antibody detected in C32 cells. C32 lysates were diluted from 2.5 μg to 156 ng. Lysates were separated on 4-12% Bis-Tris gels, electrophoresed at 200V for 45 minutes in MES Running Buffer, and transferred to nitrocellulose membranes in Tris-Glycine buffer at 100V for 65 minutes. Blots were blocked in Intercept® (PBS) Blocking Buffer (P/N 927-70001), probed with Vinculin Rabbit Monoclonal Antibody, and detected on an Odyssey® CLx Imager.
在 C32 细胞中检测到 Vinculin Rabbit Monoclonal Antibody。 C32 裂解物从 2.5 μg 稀释至 156 ng。 在 4-12% Bis-Tris 凝胶上分离裂解物，在 MES Running Buffer 中以 200V 电泳 45 分钟，然后在 100V 的 Tris-Glycine 缓冲液中转移至硝酸纤维素膜 65 分钟。 在 Intercept® (PBS) 封闭缓冲液（P/N 927-70001）中封闭印迹，用 Vinculin 兔单克隆抗体探测，并在 Odyssey® CLx Imager 上检测。

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