Reagents > VRDye™ 549 Streptavidin,VRDye™ 549 链霉亲和素
VRDye 549 Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 mM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
VRDye 549 链霉亲和素以液体形式提供，缓冲液中含有 10 mM 磷酸盐、183 mM NaCl、2.7 mM KCl，pH 7.4，叠氮化钠 0.005% (w/v) 作为防腐剂。
要使用，请在使用前短暂离心以消除可能在溶液中形成的聚集体。 这将减少非特异性背景染色。
对于基于膜的应用和 In-Gel Western，建议在检测孵育步骤中添加 SDS（0.02% 至 0.1% 最终浓度）和 Tween® 20（0.1% 至 0.2% 最终浓度）以减少非 特定背景染色。
Recommended Dilutions
| Application | Suggested Range | Tween 20* | SDS* |
|---|---|---|---|
| Western blot detection | 1:2,000 – 1:5,000 | 0.1 – 0.2% (v/v) | 0.02 – 0.1% (v/v) |
| Other | User optimized | User optimized | User optimized |
Optimum dilutions will vary and should be determined empirically.
* Added to reduce non-specific background staining.